Bacterial persistence is recognized as a major cause of antibiotic therapy failure,causing biofilms and chronic intractable infections.The emergence of persisters in K.pneumoniae isolates has become a worldwide public...Bacterial persistence is recognized as a major cause of antibiotic therapy failure,causing biofilms and chronic intractable infections.The emergence of persisters in K.pneumoniae isolates has become a worldwide public health concern.Despite this clinical threat,currently,there are no viable means for eradicating K.pneumoniae persisters.In this project,chromium oxide(Cr_(2)O_(3))nanoparticles were synthesized by the photochemical method.The morphology,topographic and physical properties of nano-synthesized were described by transmission electron microscopy(TEM),atomic force microscopy(AFM),X-ray powder diffraction(XRD),and ultraviolet-visible spectroscopy(UV.vis)measurements.The obtained average size of Cr_(2)O_(3)-NPs was to be ranging from 11 to 30 nm.The activities of the Cr_(2)O_(3)-NPs for antibacterial and antibiofilm formation against persistent K.pneumoniae were assessed.The result showed a significant inhibitory effect of Cr_(2)O_(3)-NPs against K.pneumoniae.A,where the zones of inhibition were 12-18 mm,and the minimum inhibitory concentration(MIC)was 625μg/mL.The concentration of Cr_(2)O_(3)-NPs of 10 mg/mL demonstrated the highest inhibition activity against biofilm formation(73.95±2.17%),indicating the lowest inhibition(19.08±1.32%)at a level of 0.625 mg/mL.Cr_(2)O_(3)-NPs therefore had a positive impact on biofilms that were produced by persistence isolates of K.pneumoniae.展开更多
文摘Bacterial persistence is recognized as a major cause of antibiotic therapy failure,causing biofilms and chronic intractable infections.The emergence of persisters in K.pneumoniae isolates has become a worldwide public health concern.Despite this clinical threat,currently,there are no viable means for eradicating K.pneumoniae persisters.In this project,chromium oxide(Cr_(2)O_(3))nanoparticles were synthesized by the photochemical method.The morphology,topographic and physical properties of nano-synthesized were described by transmission electron microscopy(TEM),atomic force microscopy(AFM),X-ray powder diffraction(XRD),and ultraviolet-visible spectroscopy(UV.vis)measurements.The obtained average size of Cr_(2)O_(3)-NPs was to be ranging from 11 to 30 nm.The activities of the Cr_(2)O_(3)-NPs for antibacterial and antibiofilm formation against persistent K.pneumoniae were assessed.The result showed a significant inhibitory effect of Cr_(2)O_(3)-NPs against K.pneumoniae.A,where the zones of inhibition were 12-18 mm,and the minimum inhibitory concentration(MIC)was 625μg/mL.The concentration of Cr_(2)O_(3)-NPs of 10 mg/mL demonstrated the highest inhibition activity against biofilm formation(73.95±2.17%),indicating the lowest inhibition(19.08±1.32%)at a level of 0.625 mg/mL.Cr_(2)O_(3)-NPs therefore had a positive impact on biofilms that were produced by persistence isolates of K.pneumoniae.
