fter the alcoholic group of serine located in the binding site of Anti-HumanIgM(Fcμ) (Rabbit IgG) was selectively activated by phenylmethylsulfonyl fluoride(PMSF) and displaced with hydrogen selenide(H2Se) , the seri...fter the alcoholic group of serine located in the binding site of Anti-HumanIgM(Fcμ) (Rabbit IgG) was selectively activated by phenylmethylsulfonyl fluoride(PMSF) and displaced with hydrogen selenide(H2Se) , the serine residue was con-verted to selenocysteine(SeCys). Because SeCys is a catalytic group of glutathioneperoxidase (GPX), the mutated antibody has the GPX activity which is seventytimes more than that of PZ51, the best GPX mimic in the world. Moreover, themutated antibody remains its original antibody titer.展开更多
文摘fter the alcoholic group of serine located in the binding site of Anti-HumanIgM(Fcμ) (Rabbit IgG) was selectively activated by phenylmethylsulfonyl fluoride(PMSF) and displaced with hydrogen selenide(H2Se) , the serine residue was con-verted to selenocysteine(SeCys). Because SeCys is a catalytic group of glutathioneperoxidase (GPX), the mutated antibody has the GPX activity which is seventytimes more than that of PZ51, the best GPX mimic in the world. Moreover, themutated antibody remains its original antibody titer.
