Soybean rust caused by Phakopsora pachyrhizy is an important disease of soybean, and yield losses are very common in humid growing regions. Most commercial cultivars are susceptible and the disease is usually...Soybean rust caused by Phakopsora pachyrhizy is an important disease of soybean, and yield losses are very common in humid growing regions. Most commercial cultivars are susceptible and the disease is usually managed with fungicides. Resistance breeding is the most effective in controlling the disease. In this study, detached - leaf assay and greenhouse inoculation were used to screen 200 Glycine soja accessions for resistance to Phakopsora pachyrhizi in China. Most of the accessions were susceptible, and W8214 was the only accession that demonstrated RB resistance to Phakopsora pachyrhizy. The further repeated tests confirmed the resistance in W8214.展开更多
Asian soybean rust,caused by Phakopsora pachyrhizi,is a devastating fungal disease threatening global soybean production,particularly in tropical regions where chemical control is increasingly unsustainable.This study...Asian soybean rust,caused by Phakopsora pachyrhizi,is a devastating fungal disease threatening global soybean production,particularly in tropical regions where chemical control is increasingly unsustainable.This study employed cutting-edge 4D-DIA proteomics to investigate molecular defense mechanisms in resistant(SX6907)and susceptible(Tianlong 1)soybean cultivars during early infection(12 hpi and 3 dpi).We identified 12,852 proteins,with 1,510 differentially expressed proteins(DEPs)revealing genotype-specific responses.Resistant plants exhibited sustained upregulation of immune receptors(CRKs,LRR-RLKs),MAPK signaling components,and cell wall reinforcement proteins(peroxidases,XTHs),alongside dynamic modulation of calcium signaling and ROS homeostasis.These patterns suggest key pathways enriched in resistance may include phenylpropanoid biosynthesis,isoflavonoid production,and ER stress responses,while susceptible plants showed suppression of photosynthesis and defense pathways.Weighted Protein Co-expression Network Analysis(WPCNA)highlighted co-expression modules linked to resistance,potentially including NLR-mediated effector-triggered immunity.Crucially,DIR proteins and organelle-specific defense hubs(e.g.,chloroplasts,nuclei)were implicated in rust resistance.Validation by qPCR confirmed concordance for 84%of tested DEPs.Our findings provide a protein-level blueprint of soybean rust resistance,identifying candidate targets for marker-assisted breeding and genetic engineering to develop durable resistant varieties,reducing reliance on fungicides.展开更多
文摘Soybean rust caused by Phakopsora pachyrhizy is an important disease of soybean, and yield losses are very common in humid growing regions. Most commercial cultivars are susceptible and the disease is usually managed with fungicides. Resistance breeding is the most effective in controlling the disease. In this study, detached - leaf assay and greenhouse inoculation were used to screen 200 Glycine soja accessions for resistance to Phakopsora pachyrhizi in China. Most of the accessions were susceptible, and W8214 was the only accession that demonstrated RB resistance to Phakopsora pachyrhizy. The further repeated tests confirmed the resistance in W8214.
基金supported by the National Key Research and Development Program of China(2022YFF1001504)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2022-OCRI)+1 种基金the Basic research fund of Oil Crop Research Institute,Chinese Academy of Agricultural Sciences(1610172022002)Hubei Province Technological Innovation Program(2024BBB003).
文摘Asian soybean rust,caused by Phakopsora pachyrhizi,is a devastating fungal disease threatening global soybean production,particularly in tropical regions where chemical control is increasingly unsustainable.This study employed cutting-edge 4D-DIA proteomics to investigate molecular defense mechanisms in resistant(SX6907)and susceptible(Tianlong 1)soybean cultivars during early infection(12 hpi and 3 dpi).We identified 12,852 proteins,with 1,510 differentially expressed proteins(DEPs)revealing genotype-specific responses.Resistant plants exhibited sustained upregulation of immune receptors(CRKs,LRR-RLKs),MAPK signaling components,and cell wall reinforcement proteins(peroxidases,XTHs),alongside dynamic modulation of calcium signaling and ROS homeostasis.These patterns suggest key pathways enriched in resistance may include phenylpropanoid biosynthesis,isoflavonoid production,and ER stress responses,while susceptible plants showed suppression of photosynthesis and defense pathways.Weighted Protein Co-expression Network Analysis(WPCNA)highlighted co-expression modules linked to resistance,potentially including NLR-mediated effector-triggered immunity.Crucially,DIR proteins and organelle-specific defense hubs(e.g.,chloroplasts,nuclei)were implicated in rust resistance.Validation by qPCR confirmed concordance for 84%of tested DEPs.Our findings provide a protein-level blueprint of soybean rust resistance,identifying candidate targets for marker-assisted breeding and genetic engineering to develop durable resistant varieties,reducing reliance on fungicides.
