During a study of fungal diversity in the Puruogangri Glacier ecosystem on the Qinghai-Xizang Plateau,a strain was isolated from soil samples at the glacier forefields using the dilution plate method,and identified as...During a study of fungal diversity in the Puruogangri Glacier ecosystem on the Qinghai-Xizang Plateau,a strain was isolated from soil samples at the glacier forefields using the dilution plate method,and identified as Sinophaeosphaeria xizangensis gen.et sp.nov.Morphological analysis revealed that the fungus produced olive to olive-brown conidia on PDA medium.The conidia consist of two or more cells,arranged in curved or irregular chains.Some cells slightly swollen,forming distinctly prominent bulging regions or aggregating into dense,hammer-shaped structures.Phylogenetic analyses based on five genetic loci,including internal transcribed spacer(ITS),large subunit ribosomal DNA(LSU),small subunit ribosomal DNA(SSU),RNA polymeraseⅡsecond largest subunit(rpb2),and translation elongation factor 1-α(tef1-α),indicate that this fungus represents a previously undescribed lineage within the family Phaeosphaeriaceae.This genus is located at the base of a branch that includes Paraloratospora,Loratospora,Wingfieldomyces and Sulcispora,revealing its unique evolutionary position.展开更多
基金supported by the Second Xizang Plateau Scientific Expedition and Research Program(2024QZKK02010303)the Second Qinghai-Xizang Plateau Scientific Expedition and Research(STEP)Program(2019QZKK0503).
文摘During a study of fungal diversity in the Puruogangri Glacier ecosystem on the Qinghai-Xizang Plateau,a strain was isolated from soil samples at the glacier forefields using the dilution plate method,and identified as Sinophaeosphaeria xizangensis gen.et sp.nov.Morphological analysis revealed that the fungus produced olive to olive-brown conidia on PDA medium.The conidia consist of two or more cells,arranged in curved or irregular chains.Some cells slightly swollen,forming distinctly prominent bulging regions or aggregating into dense,hammer-shaped structures.Phylogenetic analyses based on five genetic loci,including internal transcribed spacer(ITS),large subunit ribosomal DNA(LSU),small subunit ribosomal DNA(SSU),RNA polymeraseⅡsecond largest subunit(rpb2),and translation elongation factor 1-α(tef1-α),indicate that this fungus represents a previously undescribed lineage within the family Phaeosphaeriaceae.This genus is located at the base of a branch that includes Paraloratospora,Loratospora,Wingfieldomyces and Sulcispora,revealing its unique evolutionary position.