Objectives:Petasites japonicus(PJ)is a traditional medicinal herb widely used in East Asia for treating diverse ailments.However,its anticancer properties and underlying mechanisms have not been elucidated.This study ...Objectives:Petasites japonicus(PJ)is a traditional medicinal herb widely used in East Asia for treating diverse ailments.However,its anticancer properties and underlying mechanisms have not been elucidated.This study investigated the anticancer potential and molecular mechanisms of the methanol extract of Petasites japonicus(PJE)in human adenocarcinoma gastric stomach(AGS)cells.Methods:AGS cells were treated with various concentrations of PJE,and cell viability was measured using MTT and CCK-8 assays.Apoptotic cell death was evaluated by the cell cycle,caspase-3 and-9 activity assays,and western blotting.To elucidate the underlying signaling mechanisms,we also examined the generation of reactive oxygen species(ROS)and the activation of mitogen-activated protein kinase(MAPK).Results:PJE significantly decreased AGS cell viability and increased the sub-G1 population,indicating apoptosis.PJE upregulated Bcl-2-associated X protein(Bax)expression while downregulating B-cell lymphoma 2(Bcl-2)and surviving.Increased cleavage of caspase-3,caspase-9,and poly(ADP-ribose)polymerase(PARP)-1 confirmed the activation of the intrinsic apoptotic pathway.Moreover,PJE induced phosphorylation of MAPKs and induced a dose-dependent increase in ROS generation.Conclusions:PJE triggers apoptosis in gastric cancer cells through ROS-dependent mitochondrial and MAPK signaling,leading to potent anticancer effects.These findings highlight PJ as a promising natural source for developing new therapeutic agents for gastric cancer.展开更多
A new bakkenolide-type compound, bakkenolide-Va（1）, was isolated from the rhizome of Petasites tricholobus. The structure was characterized on the basis of various NMR （1H, 13C, 1H-1H COSY, HMQC, HMBC and NOESY） a...A new bakkenolide-type compound, bakkenolide-Va（1）, was isolated from the rhizome of Petasites tricholobus. The structure was characterized on the basis of various NMR （1H, 13C, 1H-1H COSY, HMQC, HMBC and NOESY） and mass spectrometry data. The neuroprotective activity of bakkenolide-Va was investigated in primarily cultured neurons treated by hypoxia-ghicose deprivation and various types of oxidative insults.展开更多
A novel pentacyclic triterpenoid, petatrichol B, was isolated from the rhizome of Petasites tricholobus. Its structure was established by means of spectroscopic analysis （EIMS, HRSIMS, IR, 1D NMR and 2D NMR）.
A novel triterpenoid, D:B-friedoursane-3α, 16α-dihydroxy-7α, 8α-epoxy-5(10)-ene, named petatrichol A, was isolated from the roots of Petasites tricholobus Franch.. Its structure was elucidated by spectroscopic met...A novel triterpenoid, D:B-friedoursane-3α, 16α-dihydroxy-7α, 8α-epoxy-5(10)-ene, named petatrichol A, was isolated from the roots of Petasites tricholobus Franch.. Its structure was elucidated by spectroscopic methods, especially 2DNMR techniques.展开更多
A high-performance liquid chromatography coupled with variable wavelength detection(HPLC–UV)was developed to evaluate the quality of Petasites tatewakianus Kitam through a simultaneous determination of eight bakkenol...A high-performance liquid chromatography coupled with variable wavelength detection(HPLC–UV)was developed to evaluate the quality of Petasites tatewakianus Kitam through a simultaneous determination of eight bakkenolides:bakkenolide-L,bakkenolide-D,bakkenolide-B,bakkenolide-Ⅰa,bakkenolide-Ⅶa,bakkenolide-Ⅳa,bakkenolide-Ⅲa and homofukinolide.With a C18 analytical column,the eight analytes were efficiently separated using tetrahydrofuran–acetonitrile–water as the mobile phase in a constant program.The limits of detection and limits of quantitation of the method ranged 0.42–2.56μg/mL and 1.22–8.40μg/mL,respectively.The intra-and inter-day precisions of the method were all less than 1.83%.All the recoveries for the spiked analytes ranged 97.8%–102.4%.There were statistically significant differences among the contents of the eight bakkenolides in different parts and origins of P.tatewakianus(Po0.01).The content of total bakkenolides in rhizome was higher than that in other parts of the plant.The content of total bakkenolides in rhizome from Baishan was higher than those in other localities.The result suggested that rhizome may be the most valuable part of P.tatewakianus,and P.tatewakianus from Baishan may be the best plant resource.Our results might serve as a sound foundation for further study and application of this plant.展开更多
基金supported by a 2-Year Research Grant of Pusan National University。
文摘Objectives:Petasites japonicus(PJ)is a traditional medicinal herb widely used in East Asia for treating diverse ailments.However,its anticancer properties and underlying mechanisms have not been elucidated.This study investigated the anticancer potential and molecular mechanisms of the methanol extract of Petasites japonicus(PJE)in human adenocarcinoma gastric stomach(AGS)cells.Methods:AGS cells were treated with various concentrations of PJE,and cell viability was measured using MTT and CCK-8 assays.Apoptotic cell death was evaluated by the cell cycle,caspase-3 and-9 activity assays,and western blotting.To elucidate the underlying signaling mechanisms,we also examined the generation of reactive oxygen species(ROS)and the activation of mitogen-activated protein kinase(MAPK).Results:PJE significantly decreased AGS cell viability and increased the sub-G1 population,indicating apoptosis.PJE upregulated Bcl-2-associated X protein(Bax)expression while downregulating B-cell lymphoma 2(Bcl-2)and surviving.Increased cleavage of caspase-3,caspase-9,and poly(ADP-ribose)polymerase(PARP)-1 confirmed the activation of the intrinsic apoptotic pathway.Moreover,PJE induced phosphorylation of MAPKs and induced a dose-dependent increase in ROS generation.Conclusions:PJE triggers apoptosis in gastric cancer cells through ROS-dependent mitochondrial and MAPK signaling,leading to potent anticancer effects.These findings highlight PJ as a promising natural source for developing new therapeutic agents for gastric cancer.
文摘A new bakkenolide-type compound, bakkenolide-Va（1）, was isolated from the rhizome of Petasites tricholobus. The structure was characterized on the basis of various NMR （1H, 13C, 1H-1H COSY, HMQC, HMBC and NOESY） and mass spectrometry data. The neuroprotective activity of bakkenolide-Va was investigated in primarily cultured neurons treated by hypoxia-ghicose deprivation and various types of oxidative insults.
基金This work was financed by the National Natural Science Foundation of China(No．29972017)
文摘A novel pentacyclic triterpenoid, petatrichol B, was isolated from the rhizome of Petasites tricholobus. Its structure was established by means of spectroscopic analysis （EIMS, HRSIMS, IR, 1D NMR and 2D NMR）.
基金This project was suppoaed by the NNSFC(No．2997201)
文摘A novel triterpenoid, D:B-friedoursane-3α, 16α-dihydroxy-7α, 8α-epoxy-5(10)-ene, named petatrichol A, was isolated from the roots of Petasites tricholobus Franch.. Its structure was elucidated by spectroscopic methods, especially 2DNMR techniques.
文摘A high-performance liquid chromatography coupled with variable wavelength detection(HPLC–UV)was developed to evaluate the quality of Petasites tatewakianus Kitam through a simultaneous determination of eight bakkenolides:bakkenolide-L,bakkenolide-D,bakkenolide-B,bakkenolide-Ⅰa,bakkenolide-Ⅶa,bakkenolide-Ⅳa,bakkenolide-Ⅲa and homofukinolide.With a C18 analytical column,the eight analytes were efficiently separated using tetrahydrofuran–acetonitrile–water as the mobile phase in a constant program.The limits of detection and limits of quantitation of the method ranged 0.42–2.56μg/mL and 1.22–8.40μg/mL,respectively.The intra-and inter-day precisions of the method were all less than 1.83%.All the recoveries for the spiked analytes ranged 97.8%–102.4%.There were statistically significant differences among the contents of the eight bakkenolides in different parts and origins of P.tatewakianus(Po0.01).The content of total bakkenolides in rhizome was higher than that in other parts of the plant.The content of total bakkenolides in rhizome from Baishan was higher than those in other localities.The result suggested that rhizome may be the most valuable part of P.tatewakianus,and P.tatewakianus from Baishan may be the best plant resource.Our results might serve as a sound foundation for further study and application of this plant.
