[Objective] This study aimed to explore the molecular mechanism of senescence in ethylene-insensitive flowers. [Method] The dahlia petals were used as matedal, and the senescence-associated proteins were isolated and ...[Objective] This study aimed to explore the molecular mechanism of senescence in ethylene-insensitive flowers. [Method] The dahlia petals were used as matedal, and the senescence-associated proteins were isolated and identified using two-dimensional electrophoresis, mass spectrometry and an encoding gene was cloned using molecular biology techniques. [Result] In the two-dimensional elec- trophorogram of proteins from dahlia petals at building color, full flowering and flow- er senescence pedods, a total of 44 protein spots with differences in expression level more than two times were detected. From the 44 protein spots, xyloglucan （XTHs）, a senescence-associated protein, was iso- lated and identified and its expression level was increased continuously with the senescence process of dahlia petals. By using the total RNA of dahlia petals as matedal and a pair of degenerate pdmers, the cDNA sequence of XTH gene was cloned by RT-PCR. The encoding region of XTH gene has a full length of 882 bp, encoding 293 amino acid residues, and is named as DpXTH1 （Accession number： HM053613.1）. The cluster analysis showed that the amino acid sequence of DpXTH1 has high homology with those of XTHs in other plants. [Conclusion] The isolated and identified DpXTH1 from dahlia petals belonged to the XTH family in plants, and its biological function was associated with the senescence process and regulation of dahlia petals.展开更多
The aim of the present work was to investigate the synergetic effect of microwave and ultrasound treatment on the production, chemical composition and antioxidant activity of rose essential oil. The rose essential oil...The aim of the present work was to investigate the synergetic effect of microwave and ultrasound treatment on the production, chemical composition and antioxidant activity of rose essential oil. The rose essential oil was extracted from fresh Rosa x damciscena Mill. petals by four methods, hydrodistillation, steam distillation, organic solvent extraction and ultrasounds followed by microwave hydrodistillation. The chemical composition of the extracts was analysed by GC-MS, and the antioxidant capacity by DPPH. It was found that both chemical composition and the antioxidant activity of the extracts depend on the extraction method. Overall it was found that microwaves coupled with ultrasonic treatment can be used effectively for the intensification of the extraction of monoterpenes and sesquiterpenes--fragrance bearing molecules--and equally, for increased antioxidant activity while using about 4 time shorter extraction time. The scale-up of the method was also evaluated. The results obtained in this research support the possible use of the US/MW method for the extraction of rose essential oil for the pharmaceutical and fragrance industry.展开更多
From yellow petals of Blue Mahoe, besides the known flavonoids gossypetin and gossypitrin, a new one gossypetin derivative was isolated from ethanolic extracts after Soxhlet extraction. Hence, in this study, we presen...From yellow petals of Blue Mahoe, besides the known flavonoids gossypetin and gossypitrin, a new one gossypetin derivative was isolated from ethanolic extracts after Soxhlet extraction. Hence, in this study, we present a validated, sensitive and reliable NMR method for the simultaneous identification of flavonoids in this flower drug. Structure analyses of this flavonoid, revealed the identical glycoside moiety attached to a flavonol skeleton like gossypitrin, for which the structure of gossypetin-3'-O-β-glucoside was deduced from extensive NMR experiments.展开更多
Flower petals have not only diverse macroscopic morphologies but are rich in microscopic surface patterns, which are crucial to their biological functions. Both experimental measurements and theoretical analysis are c...Flower petals have not only diverse macroscopic morphologies but are rich in microscopic surface patterns, which are crucial to their biological functions. Both experimental measurements and theoretical analysis are conducted to reveal the physical mechanisms underlying the formation of minute wrinkles on flower petals. Three representative flowers, daisy, kalanchoe blossfeldiana, and Eustoma grandiflorurn, are investigated as examples. A surface wrinkling model, incorporating the measured mechanical properties and growth ratio, is used to elucidate the difference in their surface morphologies. The mismatch between the anisotropic epidermal cell growth and the isotropic secretion of surficial wax is found to dictate the surface patterns.展开更多
In this study, we provide the first evidence of two pairs of petals of the rapidly expanded and short-lived nocturnal flowers of Capparis spinosa L. （caper） during the prolonged drought period in Eastern Mediterrane...In this study, we provide the first evidence of two pairs of petals of the rapidly expanded and short-lived nocturnal flowers of Capparis spinosa L. （caper） during the prolonged drought period in Eastern Mediterranean region. The corolla of the winter-deciduous, perennial C. spinosa consists of two pairs of petals： a pair of white dis- tinct petals and a pair of connate variegated petals with green basal parts. The results indicated the presence of substantially different amounts of chlorophyll in the two pairs of petals, while their carbohydrates＇ content is com- parable with that of the green sepals. High resolution imaging of petal surfaces of short-lived flowers of C. spinosa, obtained by using scanning electron microscopy, revealed stomata on the adaxial epidermis on both the white and the green parts of the variegated petals; while dense hairs were found on the surface ofthe abaxial green parts of the variegated petals. Adaxial, epidermal cells of the variegated petals, viewed using atomic force microscopy, pos- sess a submicron, cuticular microfolding that differs between the white and the green parts of the petals. It appears that microridges on the adaxial, white parts of petals of C. spinosa compensate for an increase in cell surface area of the short lived petals, while the roughness of the green parts of petals was found to be higher than that of the white parts. Thus, the micromorphology of surfaces of epidermal cells is expected to affect optical properties and wettability of the floral tissues. These findings may be particularly important for understanding the performance of the short-lived petals of C. spinosa, which are exposed to dryland environments.展开更多
Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ...Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.展开更多
Windmills rotate gracefully,European-style buildings stand prominently and vast fields of wildly colored tulips extend to the horizon...Such a picturesque scene can not only be spotted in the Netherlands but also in D...Windmills rotate gracefully,European-style buildings stand prominently and vast fields of wildly colored tulips extend to the horizon...Such a picturesque scene can not only be spotted in the Netherlands but also in Dafeng District,Y ancheng City of Jiangsu Province in east China,at a place called-Holland Flower Par.展开更多
For planar Newtonian 4-body problems with equal masses, we use variational methods to prove the existence of a non-collision periodic choreography solution such that all bodies move on a rose-type curve with three pet...For planar Newtonian 4-body problems with equal masses, we use variational methods to prove the existence of a non-collision periodic choreography solution such that all bodies move on a rose-type curve with three petals.展开更多
Iris sanguinea Donn ex Horn.is a common perennial ornamental herb in Northeast China due to its strong cold-resistance capacity.In this study,integrative analyses of metabolomics and transcriptomics data were performe...Iris sanguinea Donn ex Horn.is a common perennial ornamental herb in Northeast China due to its strong cold-resistance capacity.In this study,integrative analyses of metabolomics and transcriptomics data were performed on five cultivars of I.sanguinea with varying petal colors to elucidate the molecular basis underlying petal pigmentation.The metabolomics data revealed that a total of five major anthocyanins were responsible for petal pigmentation in I.sanguinea.While two delphinidin-3-O-glucoside derivatives contributed to blue coloration in petals,two petunidin-3-O-glucoside and cyanidin-3-O-rutinoside derivatives exhibited significantly higher abundances in the deep pink petals.In addition,transcriptomics revealed a gradual increase in the expression of a large set of genes involved in flavonoid and anthocyanin biosynthesis pathways,corresponding to the varying levels of anthocyanin content among cultivar petals.Integrated metabolomics and transcriptomics analysis further led to the discovery of molecular modules controlling petal pigmentation,in which an R2R3 MYB transcription factor IsMYBL1 was found to be associated with the accumulation of key anthocyanin derivatives,it localized to the nucleus.The regulatory role of IsMYBL1 in anthocyanin accumulation has been validated through overexpressing in tobacco and knockdown in I.sanguinea using an improved virusinduced gene silencing(VIGS)approach.Finally,BiFC and Y2H showed that IsMYBL1 interacts with IsEGL3 and IsTTG1 to activate the expression of genes involved in anthocyanin biosynthesis,Yeast one-hybrid,dual-luciferase assays,showed that IsMYBL1 significantly increased anthocyanin contents in I.sanguinea by promoting the expression of IsANS,thereby promoting anthocyanin accumulation.Taken together,our study has provided a comprehensive molecular profiling of petal coloration in I.sanguinea and revealed key gene/metabolite networks that determine petal pigmentation.展开更多
We propose a method to measure the flatness of an object with a petal-like pattern generated by the interference of the measured orbital angular momentum(OAM)beam and the reference OAM beam which carries the opposite ...We propose a method to measure the flatness of an object with a petal-like pattern generated by the interference of the measured orbital angular momentum(OAM)beam and the reference OAM beam which carries the opposite OAM state.By calculating the difference between the petal rotation angle without/with the object,the thickness information of the object,and then the flatness information,can be evaluated.Furthermore,the direction of the object’s flatness can be determined by the petal’s clockwise/counterclockwise rotation.We theoretically analyze the relationship between the object’s thickness and petal rotation angle,and verify the proposed method by experiment.The experimental results show that the proposed method is a high precision flatness measurement and can obtain the convex/concave property of the flatness.For the 1.02 mm glass sample,the mean deviation of the flatness is 1.357×10^(-8) and the variance is 0.242×10^(-16).For the 0.50 mm glass sample,the mean deviation of the flatness is 1.931×10^(-8) and the variance is 2.405×10^(-16).Two different topological charges are adopted for the 2.00 mm glass sample,and their flatness deviations are 0.239×10^(-8)(ℓ=1)and 0.246×10^(-8)(ℓ=2),where their variances are 0.799×10^(-18)(ℓ=1)and 0.775×10^(-18)(ℓ=2),respectively.It is shown that the flatness measured by the proposed method is the same for the same sample when different topological charges are used.All results indicate that the proposed method may provide a high flatness measurement,and will be a promising way to measure the flatness.展开更多
Poor resolution for the identification of endopeptidase(EP) activity in activity gel assays is a critical issue in the analysis of the postharvest physiology of rose petals. In this paper, major factors influencing EP...Poor resolution for the identification of endopeptidase(EP) activity in activity gel assays is a critical issue in the analysis of the postharvest physiology of rose petals. In this paper, major factors influencing EP activity gel assays were evaluated. The results showed that a phosphate(NaH_2PO_4/Na_2HPO_4) buffer favors the detection of clear EP bands, as compared to Tris–HCl buffer. Removal of salts and pigments with Sephadex G-25 columns was vital to the measurement of EP activity in rose petal extracts. For optimal resolution of bands, we show that before electrophoresis,samples should be treated for 10 min at 40 °C. Additionally, electrophoresis should be done in 12% SDS–PAGE co-polymerized with 0.15%(w/v) gelatin. After electrophoresis, the optimal incubation temperature and pH are 42 °C and 7.0, respectively. Using our optimized assay, Rh-EP1,Rh-EP2, Rh-EP3, three proteases with molecular masses of 200, 123.5, and 97.4 kD, respectively, were detected in rose petals. Experiments using EP class-specific inhibitors revealed that Rh-EP2 and Rh-EP3 were both serine proteases, while Rh-EP1 was a metalloprotease. In this study, we also measured changes in EP activity during flower opening, senescence, and water deficit stress(WDS) using our optimized activity gel assay,and found that Rh-EP3 may be more relevant to senescence in roses compared with Rh-EP1 or Rh-EP2. Changes occurring to EPs after WDS were similar to those during the period from flower opening to senescence, and Rh-EP3 activities were greatly increased by WDS treatment. Collectively, our results suggest that significant increases in Rh-EPs activities, especially increases in Rh-EP3 activity, may contribute to the flower senescence induced under WDS treatment.展开更多
Sunflower petal is one of the parts of the sunflower which has drawn attention and has several applications these days.These applications justify getting information about physical properties,mechanical properties,dry...Sunflower petal is one of the parts of the sunflower which has drawn attention and has several applications these days.These applications justify getting information about physical properties,mechanical properties,drying trends,etc.in order to design new machines and use new methods to harvest or dry the sunflower petals.For three varieties of sunflower,picking force of petals was measured;number of petals of each head was counted;unit mass and 1000-unit mass of fresh petals were measured and length,width,and projected area of fresh petals were calculated based on image processing technique;frequency distributions of these parameters were modeled using statistical distribution models namely Gamma,Generalized Extreme Value(G.E.V),Lognormal,and Weibull.Results of picking force showed that with increasing number of days after appearing the first petal on each head from 5 to 14 and decreasing loading rate from 150 gmin^-1 to 50 g min^-1 values of picking force were decreased for three varieties,but diameter of sunflower head had different effects on picking force for each variety.Length,width,and number of petals of Dorsefid variety ranged from 38.52 to 95.44 mm,3.80 to 9.28mm and 29 to 89,respectively.The corresponding values ranged from 34.19 to 88.18 mm,4.28 to 10.60 mm and 21 to 89,respectively for Shamshiri variety and ranged from 44.47 to 114.63 mm,7.03 to 20.31 mm and 29 to 89 for Sirena variety.Results of frequency distribution modeling indicated that in most cases,G.E.V and Weibull distributions had better performance than other distributions.展开更多
The double flower developmental process is regulated via a complex transcriptional regulatory network.To understand this highly dynamic and complex developmental process of Dianthus spp.,we performed a comparative ana...The double flower developmental process is regulated via a complex transcriptional regulatory network.To understand this highly dynamic and complex developmental process of Dianthus spp.,we performed a comparative analysis of floral morphology and transcriptome dynamics in simple flowers and double flowers.We found that the primordium of double flowers of‘X’was larger in size compared to that of simple flowers of‘L’in Dianthus chinensis.RNA-seq and Weighted Gene Co-expression Network Analysis(WGCNA)during flower development,identified stage-specific gene network modules.Expression analysis by RNA-seq indicated that a group of genes related to floral meristem identity,primordia position and polarity were highly expressed in double flowers genotypes compared to simple flowers genotypes,suggesting their roles in double-petal formation.A total of 21 DEGs related to petal number were identified between simple and double flowers.The experiments of in situ hybridization revealed that DcaAP2L,DcaLFY and DcaUFO genes were expressed in the intra-sepal boundary and petal boundary.We proposed a potential transcriptional regulatory network for simple and double flower development.This study provides novel insights into the molecular mechanism underlying double flower formation in Dianthus spp.展开更多
Pigmentation patterns are ubiquitous in nature.Visually striking pigmentation patterns are not only aesthetically appealing,but also crucial to pollinator interaction and plant fitness.The formation of complex floral ...Pigmentation patterns are ubiquitous in nature.Visually striking pigmentation patterns are not only aesthetically appealing,but also crucial to pollinator interaction and plant fitness.The formation of complex floral pigmentation patterns mainly relies on the spatiotemporal expression of R2R3-MYB transcription factors and is often associated with certain floral development programs,such as floral organ identity,symmetry,which likely provide key information to initiate the patterning.For a complex pigmentation pattern to form,at least a pair of activator and inhibitor is required,despite their interaction might vary depending on the system being investigated.The regulation of pigmentation pattern involves multiple molecular mechanisms,such as transcriptional regulation,small RNA,transposon-mediated gene silencing,and methylation of gene body.Identifying these regulators can be facilitated by using single-cell and spatial transcriptomics as well as innovative plant transformation technologies.Moreover,plant organ development and pigmentation patterns are often interdependent,but current methods of describing patterns are static.Therefore,more precise and quantitative measurements are needed to elucidate the developmental mechanisms underlying complex pigmentation patterns in flowers.展开更多
The red petals of the flowers of Talipariti elatum, commonly named majagua is used as antitussive, expectorant and antasthmatic in phytotherapy, although the plants’ composition has not been determined in detail so f...The red petals of the flowers of Talipariti elatum, commonly named majagua is used as antitussive, expectorant and antasthmatic in phytotherapy, although the plants’ composition has not been determined in detail so far. Hence, in this study, we present a validated, sensitive, reliable, and cheap narrow-bore LC-UV-ESI-MS/MS coupled to PDA (photodiode array detectors) method for the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, the structures of two compounds have been elucidated by LC-MS experiments after isolation. Structure analyses allow proposing the presence of gossypitrin (gossypetin-7-O-glucoside) or gossypetin-3'-O-glucoside, a quercetin derivative, possibly quercetin-3-O-glucoside and an unidentified compound with an impair number of m/z, probably an alkaloid.展开更多
[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemu...[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemum rnorifolium petal using Trizol reagent, and the cDNA fragment of XTH gene was cloned by RT-PCR and T/A cloning. [Result] The sequencing result showed that the cloned cDNA sequence was 911 bp. It was predicted to encode a polypeptide of 293 amino acids and had seven active sites of XTH family, and then named as CmXTH （gene accession number HM752243）. In addition, The BLAST analysis showed that the deduced amino acid sequence of CmXTH showed high homology with other 19 chosen plant XTHs. Among of these, CmXTH shared closer genetic relationship with Gerbera jamesonii, Solanum lycopersicum, whereas had relatively distant relationship with Populus euparatica, Fragaria ananassa, Actinidia deliciosa, etc. [Conclusion] The cloned fragment was certainly the cDNA of XTH gene, which was associated with the petal growth and senescence in Chrysanthemum morifolium.展开更多
The study of micromycetes infestation on flower buds, flower petals and seeds from 10 taxa of Rhododendron sp. was conducted during 2010-2012. Mycological analysis was comprised of 1,500 specimens of buds and petals a...The study of micromycetes infestation on flower buds, flower petals and seeds from 10 taxa of Rhododendron sp. was conducted during 2010-2012. Mycological analysis was comprised of 1,500 specimens of buds and petals and 500 seeds of Rhododendron sp.. It was shown that the necrosis and dieback of buds were caused by complex micromycetes （43 species）, with dominants Pestalotiopsis sydowiana, Alternaria alternata, Truncatella truncata and Epicoccum nigrum. Watery brown spots on the flower petals, resulting in the dieback of flowers, were caused by 38 species, including the most common P. sydowiana, A. alternata and Trichoderma viride. The seeds were contaminated by 18 species, and in addition to the above, the following species were associated： Oidiodendron tennuissimum, Davidiella macrocarpa and Phoma leveillei. The results of the mycological analysis confirmed the diversity spectrum of micromycetes species that inhabit the infested Rhododendron buds, flowers and seeds. The research revealed which taxa attracted the largest number of colonies and species of fungi.展开更多
Genus Talipariti has long history of use in various traditional medicine therapeutic applications in Cuba, especially intreatment of bronchial asthma and flu. Petals of genus Talipariti are rich source of secondary me...Genus Talipariti has long history of use in various traditional medicine therapeutic applications in Cuba, especially intreatment of bronchial asthma and flu. Petals of genus Talipariti are rich source of secondary metabolites and most of these metabolitesare reported to possess expectorant, antasthmatic, appetitive, antioxidant, and antibacterial properties. Hence, in our study, we present avalidated, sensitive, reliable, and cheap narrow-bore UHPLC-UV-ESI-MS/MS coupled to PDA （photodiode array detectors） methodfor the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, abouttwelve compounds were identified in this specie based on chromatography retention time （tR）, UV and MS/MS spectra and comparedwith those of isolated authentic compound and literature data. About eighth constituents were reported for the first time from Taliparitielatum. Our results demonstrated the developed method could be employed as a rapid and versatile analytical technique foridentification of chemical constituents and quality control of Talipariti elatum.展开更多
基金Supported by Applied Basic Research Project of Suzhou City(SYN201405)National College Students’Innovative Entrepreneurial Training Program(201410285049)~~
文摘[Objective] This study aimed to explore the molecular mechanism of senescence in ethylene-insensitive flowers. [Method] The dahlia petals were used as matedal, and the senescence-associated proteins were isolated and identified using two-dimensional electrophoresis, mass spectrometry and an encoding gene was cloned using molecular biology techniques. [Result] In the two-dimensional elec- trophorogram of proteins from dahlia petals at building color, full flowering and flow- er senescence pedods, a total of 44 protein spots with differences in expression level more than two times were detected. From the 44 protein spots, xyloglucan （XTHs）, a senescence-associated protein, was iso- lated and identified and its expression level was increased continuously with the senescence process of dahlia petals. By using the total RNA of dahlia petals as matedal and a pair of degenerate pdmers, the cDNA sequence of XTH gene was cloned by RT-PCR. The encoding region of XTH gene has a full length of 882 bp, encoding 293 amino acid residues, and is named as DpXTH1 （Accession number： HM053613.1）. The cluster analysis showed that the amino acid sequence of DpXTH1 has high homology with those of XTHs in other plants. [Conclusion] The isolated and identified DpXTH1 from dahlia petals belonged to the XTH family in plants, and its biological function was associated with the senescence process and regulation of dahlia petals.
文摘The aim of the present work was to investigate the synergetic effect of microwave and ultrasound treatment on the production, chemical composition and antioxidant activity of rose essential oil. The rose essential oil was extracted from fresh Rosa x damciscena Mill. petals by four methods, hydrodistillation, steam distillation, organic solvent extraction and ultrasounds followed by microwave hydrodistillation. The chemical composition of the extracts was analysed by GC-MS, and the antioxidant capacity by DPPH. It was found that both chemical composition and the antioxidant activity of the extracts depend on the extraction method. Overall it was found that microwaves coupled with ultrasonic treatment can be used effectively for the intensification of the extraction of monoterpenes and sesquiterpenes--fragrance bearing molecules--and equally, for increased antioxidant activity while using about 4 time shorter extraction time. The scale-up of the method was also evaluated. The results obtained in this research support the possible use of the US/MW method for the extraction of rose essential oil for the pharmaceutical and fragrance industry.
文摘From yellow petals of Blue Mahoe, besides the known flavonoids gossypetin and gossypitrin, a new one gossypetin derivative was isolated from ethanolic extracts after Soxhlet extraction. Hence, in this study, we present a validated, sensitive and reliable NMR method for the simultaneous identification of flavonoids in this flower drug. Structure analyses of this flavonoid, revealed the identical glycoside moiety attached to a flavonol skeleton like gossypitrin, for which the structure of gossypetin-3'-O-β-glucoside was deduced from extensive NMR experiments.
基金Supports from the National Natural Science Foundation of China(11602027)the National Science Foundation for Post-doctoral Scientists of China(2016M600969)
文摘Flower petals have not only diverse macroscopic morphologies but are rich in microscopic surface patterns, which are crucial to their biological functions. Both experimental measurements and theoretical analysis are conducted to reveal the physical mechanisms underlying the formation of minute wrinkles on flower petals. Three representative flowers, daisy, kalanchoe blossfeldiana, and Eustoma grandiflorurn, are investigated as examples. A surface wrinkling model, incorporating the measured mechanical properties and growth ratio, is used to elucidate the difference in their surface morphologies. The mismatch between the anisotropic epidermal cell growth and the isotropic secretion of surficial wax is found to dictate the surface patterns.
基金supported by the research grant PENED 03174co-funded by the European Union(75%) and the Greek General Secretary of Research and Technology and Private Sectors (25%)
文摘In this study, we provide the first evidence of two pairs of petals of the rapidly expanded and short-lived nocturnal flowers of Capparis spinosa L. （caper） during the prolonged drought period in Eastern Mediterranean region. The corolla of the winter-deciduous, perennial C. spinosa consists of two pairs of petals： a pair of white dis- tinct petals and a pair of connate variegated petals with green basal parts. The results indicated the presence of substantially different amounts of chlorophyll in the two pairs of petals, while their carbohydrates＇ content is com- parable with that of the green sepals. High resolution imaging of petal surfaces of short-lived flowers of C. spinosa, obtained by using scanning electron microscopy, revealed stomata on the adaxial epidermis on both the white and the green parts of the variegated petals; while dense hairs were found on the surface ofthe abaxial green parts of the variegated petals. Adaxial, epidermal cells of the variegated petals, viewed using atomic force microscopy, pos- sess a submicron, cuticular microfolding that differs between the white and the green parts of the petals. It appears that microridges on the adaxial, white parts of petals of C. spinosa compensate for an increase in cell surface area of the short lived petals, while the roughness of the green parts of petals was found to be higher than that of the white parts. Thus, the micromorphology of surfaces of epidermal cells is expected to affect optical properties and wettability of the floral tissues. These findings may be particularly important for understanding the performance of the short-lived petals of C. spinosa, which are exposed to dryland environments.
基金Supported by Heilongjiang Province Science and Technology Research Projects (GB06B112-5)
文摘Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.
文摘Windmills rotate gracefully,European-style buildings stand prominently and vast fields of wildly colored tulips extend to the horizon...Such a picturesque scene can not only be spotted in the Netherlands but also in Dafeng District,Y ancheng City of Jiangsu Province in east China,at a place called-Holland Flower Par.
基金supported by National Natural Science Foundation of China,a grant for Ph.D. of Chinese Educational Committeethe Scientific Research Foundation of Huaiyin Institute of Technology (Grant Nos. HGB0913, HGC0928)
文摘For planar Newtonian 4-body problems with equal masses, we use variational methods to prove the existence of a non-collision periodic choreography solution such that all bodies move on a rose-type curve with three petals.
基金supported by the National Science Foundation(Grant No.31670344)the Fundamental Research Funds for the Central Universities(Grant No.2572023CT18)the Natural Fund Project of Heilongjiang Province(Grant No.LH2020C044).
文摘Iris sanguinea Donn ex Horn.is a common perennial ornamental herb in Northeast China due to its strong cold-resistance capacity.In this study,integrative analyses of metabolomics and transcriptomics data were performed on five cultivars of I.sanguinea with varying petal colors to elucidate the molecular basis underlying petal pigmentation.The metabolomics data revealed that a total of five major anthocyanins were responsible for petal pigmentation in I.sanguinea.While two delphinidin-3-O-glucoside derivatives contributed to blue coloration in petals,two petunidin-3-O-glucoside and cyanidin-3-O-rutinoside derivatives exhibited significantly higher abundances in the deep pink petals.In addition,transcriptomics revealed a gradual increase in the expression of a large set of genes involved in flavonoid and anthocyanin biosynthesis pathways,corresponding to the varying levels of anthocyanin content among cultivar petals.Integrated metabolomics and transcriptomics analysis further led to the discovery of molecular modules controlling petal pigmentation,in which an R2R3 MYB transcription factor IsMYBL1 was found to be associated with the accumulation of key anthocyanin derivatives,it localized to the nucleus.The regulatory role of IsMYBL1 in anthocyanin accumulation has been validated through overexpressing in tobacco and knockdown in I.sanguinea using an improved virusinduced gene silencing(VIGS)approach.Finally,BiFC and Y2H showed that IsMYBL1 interacts with IsEGL3 and IsTTG1 to activate the expression of genes involved in anthocyanin biosynthesis,Yeast one-hybrid,dual-luciferase assays,showed that IsMYBL1 significantly increased anthocyanin contents in I.sanguinea by promoting the expression of IsANS,thereby promoting anthocyanin accumulation.Taken together,our study has provided a comprehensive molecular profiling of petal coloration in I.sanguinea and revealed key gene/metabolite networks that determine petal pigmentation.
基金supported by the National Natural Science Foundation of China(Grant No.62375140)the Open Research Fund of National Laboratory of Solid State Microstructures(Grant No.M36055).
文摘We propose a method to measure the flatness of an object with a petal-like pattern generated by the interference of the measured orbital angular momentum(OAM)beam and the reference OAM beam which carries the opposite OAM state.By calculating the difference between the petal rotation angle without/with the object,the thickness information of the object,and then the flatness information,can be evaluated.Furthermore,the direction of the object’s flatness can be determined by the petal’s clockwise/counterclockwise rotation.We theoretically analyze the relationship between the object’s thickness and petal rotation angle,and verify the proposed method by experiment.The experimental results show that the proposed method is a high precision flatness measurement and can obtain the convex/concave property of the flatness.For the 1.02 mm glass sample,the mean deviation of the flatness is 1.357×10^(-8) and the variance is 0.242×10^(-16).For the 0.50 mm glass sample,the mean deviation of the flatness is 1.931×10^(-8) and the variance is 2.405×10^(-16).Two different topological charges are adopted for the 2.00 mm glass sample,and their flatness deviations are 0.239×10^(-8)(ℓ=1)and 0.246×10^(-8)(ℓ=2),where their variances are 0.799×10^(-18)(ℓ=1)and 0.775×10^(-18)(ℓ=2),respectively.It is shown that the flatness measured by the proposed method is the same for the same sample when different topological charges are used.All results indicate that the proposed method may provide a high flatness measurement,and will be a promising way to measure the flatness.
基金supported by grants from the National Natural Science Foundation of China(30972025,31372105)
文摘Poor resolution for the identification of endopeptidase(EP) activity in activity gel assays is a critical issue in the analysis of the postharvest physiology of rose petals. In this paper, major factors influencing EP activity gel assays were evaluated. The results showed that a phosphate(NaH_2PO_4/Na_2HPO_4) buffer favors the detection of clear EP bands, as compared to Tris–HCl buffer. Removal of salts and pigments with Sephadex G-25 columns was vital to the measurement of EP activity in rose petal extracts. For optimal resolution of bands, we show that before electrophoresis,samples should be treated for 10 min at 40 °C. Additionally, electrophoresis should be done in 12% SDS–PAGE co-polymerized with 0.15%(w/v) gelatin. After electrophoresis, the optimal incubation temperature and pH are 42 °C and 7.0, respectively. Using our optimized assay, Rh-EP1,Rh-EP2, Rh-EP3, three proteases with molecular masses of 200, 123.5, and 97.4 kD, respectively, were detected in rose petals. Experiments using EP class-specific inhibitors revealed that Rh-EP2 and Rh-EP3 were both serine proteases, while Rh-EP1 was a metalloprotease. In this study, we also measured changes in EP activity during flower opening, senescence, and water deficit stress(WDS) using our optimized activity gel assay,and found that Rh-EP3 may be more relevant to senescence in roses compared with Rh-EP1 or Rh-EP2. Changes occurring to EPs after WDS were similar to those during the period from flower opening to senescence, and Rh-EP3 activities were greatly increased by WDS treatment. Collectively, our results suggest that significant increases in Rh-EPs activities, especially increases in Rh-EP3 activity, may contribute to the flower senescence induced under WDS treatment.
文摘Sunflower petal is one of the parts of the sunflower which has drawn attention and has several applications these days.These applications justify getting information about physical properties,mechanical properties,drying trends,etc.in order to design new machines and use new methods to harvest or dry the sunflower petals.For three varieties of sunflower,picking force of petals was measured;number of petals of each head was counted;unit mass and 1000-unit mass of fresh petals were measured and length,width,and projected area of fresh petals were calculated based on image processing technique;frequency distributions of these parameters were modeled using statistical distribution models namely Gamma,Generalized Extreme Value(G.E.V),Lognormal,and Weibull.Results of picking force showed that with increasing number of days after appearing the first petal on each head from 5 to 14 and decreasing loading rate from 150 gmin^-1 to 50 g min^-1 values of picking force were decreased for three varieties,but diameter of sunflower head had different effects on picking force for each variety.Length,width,and number of petals of Dorsefid variety ranged from 38.52 to 95.44 mm,3.80 to 9.28mm and 29 to 89,respectively.The corresponding values ranged from 34.19 to 88.18 mm,4.28 to 10.60 mm and 21 to 89,respectively for Shamshiri variety and ranged from 44.47 to 114.63 mm,7.03 to 20.31 mm and 29 to 89 for Sirena variety.Results of frequency distribution modeling indicated that in most cases,G.E.V and Weibull distributions had better performance than other distributions.
基金supported by funding from National Natural Science Foundation of China(Grant Nos.32002074 and 31872135)China Postdoctoral Science Foundation(Grant No.2021M693445)。
文摘The double flower developmental process is regulated via a complex transcriptional regulatory network.To understand this highly dynamic and complex developmental process of Dianthus spp.,we performed a comparative analysis of floral morphology and transcriptome dynamics in simple flowers and double flowers.We found that the primordium of double flowers of‘X’was larger in size compared to that of simple flowers of‘L’in Dianthus chinensis.RNA-seq and Weighted Gene Co-expression Network Analysis(WGCNA)during flower development,identified stage-specific gene network modules.Expression analysis by RNA-seq indicated that a group of genes related to floral meristem identity,primordia position and polarity were highly expressed in double flowers genotypes compared to simple flowers genotypes,suggesting their roles in double-petal formation.A total of 21 DEGs related to petal number were identified between simple and double flowers.The experiments of in situ hybridization revealed that DcaAP2L,DcaLFY and DcaUFO genes were expressed in the intra-sepal boundary and petal boundary.We proposed a potential transcriptional regulatory network for simple and double flower development.This study provides novel insights into the molecular mechanism underlying double flower formation in Dianthus spp.
基金financially supported by grants from the National Natural Science Foundation of China(Grant No.32122078)the Fundamental Research Funds for the Central Universities(Grant No.YDZX2023018+1 种基金Grant No.KJYQ2022002)Nanjing Agricultural University start-up funds。
文摘Pigmentation patterns are ubiquitous in nature.Visually striking pigmentation patterns are not only aesthetically appealing,but also crucial to pollinator interaction and plant fitness.The formation of complex floral pigmentation patterns mainly relies on the spatiotemporal expression of R2R3-MYB transcription factors and is often associated with certain floral development programs,such as floral organ identity,symmetry,which likely provide key information to initiate the patterning.For a complex pigmentation pattern to form,at least a pair of activator and inhibitor is required,despite their interaction might vary depending on the system being investigated.The regulation of pigmentation pattern involves multiple molecular mechanisms,such as transcriptional regulation,small RNA,transposon-mediated gene silencing,and methylation of gene body.Identifying these regulators can be facilitated by using single-cell and spatial transcriptomics as well as innovative plant transformation technologies.Moreover,plant organ development and pigmentation patterns are often interdependent,but current methods of describing patterns are static.Therefore,more precise and quantitative measurements are needed to elucidate the developmental mechanisms underlying complex pigmentation patterns in flowers.
文摘The red petals of the flowers of Talipariti elatum, commonly named majagua is used as antitussive, expectorant and antasthmatic in phytotherapy, although the plants’ composition has not been determined in detail so far. Hence, in this study, we present a validated, sensitive, reliable, and cheap narrow-bore LC-UV-ESI-MS/MS coupled to PDA (photodiode array detectors) method for the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, the structures of two compounds have been elucidated by LC-MS experiments after isolation. Structure analyses allow proposing the presence of gossypitrin (gossypetin-7-O-glucoside) or gossypetin-3'-O-glucoside, a quercetin derivative, possibly quercetin-3-O-glucoside and an unidentified compound with an impair number of m/z, probably an alkaloid.
基金Supported by the Applied Basic Research Program of Suzhou City(SYN201205)the University Innovation Research and Training Program(101028537)~~
文摘[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemum rnorifolium petal using Trizol reagent, and the cDNA fragment of XTH gene was cloned by RT-PCR and T/A cloning. [Result] The sequencing result showed that the cloned cDNA sequence was 911 bp. It was predicted to encode a polypeptide of 293 amino acids and had seven active sites of XTH family, and then named as CmXTH （gene accession number HM752243）. In addition, The BLAST analysis showed that the deduced amino acid sequence of CmXTH showed high homology with other 19 chosen plant XTHs. Among of these, CmXTH shared closer genetic relationship with Gerbera jamesonii, Solanum lycopersicum, whereas had relatively distant relationship with Populus euparatica, Fragaria ananassa, Actinidia deliciosa, etc. [Conclusion] The cloned fragment was certainly the cDNA of XTH gene, which was associated with the petal growth and senescence in Chrysanthemum morifolium.
文摘The study of micromycetes infestation on flower buds, flower petals and seeds from 10 taxa of Rhododendron sp. was conducted during 2010-2012. Mycological analysis was comprised of 1,500 specimens of buds and petals and 500 seeds of Rhododendron sp.. It was shown that the necrosis and dieback of buds were caused by complex micromycetes （43 species）, with dominants Pestalotiopsis sydowiana, Alternaria alternata, Truncatella truncata and Epicoccum nigrum. Watery brown spots on the flower petals, resulting in the dieback of flowers, were caused by 38 species, including the most common P. sydowiana, A. alternata and Trichoderma viride. The seeds were contaminated by 18 species, and in addition to the above, the following species were associated： Oidiodendron tennuissimum, Davidiella macrocarpa and Phoma leveillei. The results of the mycological analysis confirmed the diversity spectrum of micromycetes species that inhabit the infested Rhododendron buds, flowers and seeds. The research revealed which taxa attracted the largest number of colonies and species of fungi.
文摘Genus Talipariti has long history of use in various traditional medicine therapeutic applications in Cuba, especially intreatment of bronchial asthma and flu. Petals of genus Talipariti are rich source of secondary metabolites and most of these metabolitesare reported to possess expectorant, antasthmatic, appetitive, antioxidant, and antibacterial properties. Hence, in our study, we present avalidated, sensitive, reliable, and cheap narrow-bore UHPLC-UV-ESI-MS/MS coupled to PDA （photodiode array detectors） methodfor the simultaneous isolation and identification of flavonoids and their glycosidic derivatives in this flower drug. In addition, abouttwelve compounds were identified in this specie based on chromatography retention time （tR）, UV and MS/MS spectra and comparedwith those of isolated authentic compound and literature data. About eighth constituents were reported for the first time from Taliparitielatum. Our results demonstrated the developed method could be employed as a rapid and versatile analytical technique foridentification of chemical constituents and quality control of Talipariti elatum.
