This review explores the pivotal role of circadian rhythm regulators,particularly the PER genes,in Oral Squamous Cell Carcinoma(OSCC).As key constituents of the biological clock,PERs exhibit a downregulated expression...This review explores the pivotal role of circadian rhythm regulators,particularly the PER genes,in Oral Squamous Cell Carcinoma(OSCC).As key constituents of the biological clock,PERs exhibit a downregulated expression pattern in OSCC,and the expression levels of PERs in OSCC patients are correlated with a favorable prognosis.PERs impact the occurrence and development of OSCC through multiple pathways.In the regulation of cell proliferation,they can function not only through cell cycle regultion but also via metabolic pathways.For example,PER1 can interact with receptors for activated C kinase 1(RACK1)and phosphatidylinositol 3-kinase(PI3K)through its PAS domain to inhibit glycolysis and thereby reduce cell proliferation.Regarding the regulation of cell death,PERs mediate various types of cell death in OSCC cells,such as p53-dependent apoptosis,protein kinase B(AKT)/mammalian target of rapamycin(mTOR)dependent autophagy,or hypoxia-inducible factor l-alpha(HIF-1a)mediated ferroptosis.In regulating epithelia-mesenchymal transition(EMT),PERs can lead to the downregulation of EMT related genes,such as zinc finger E-box binding homeobox 1/2(ZEBI/2),twist family BHLH transcription factor 1/2(TWIST1/2),and Vimentin,thereby influencing the migration and invasion capabilities of OSCC cells.In tumor angiogenesis,PERs exert regulatory effects on related factors,such as methionyl aminopeptidase 2(MetAP2)and vascular endothelial growth factor(VEGF).In the tumor immune microenvironment,PERs can inhibit the inhibitor of kappa B kinase(IKK)/nuclear factor kappa B(NF-kB)pathway and programmed cell death ligand 1(PD-L1)expression,thereby enhancing the cytotoxic effect of CD8+T cells on OSCC cells.In-depth studies focusing on elucidating the precise regulatory mechanisms of PERs can facilitate the development of therapeutic strategies targeting PERs,including restoration of PERs expression/activity,targeting PERs-regulated pathways,combination therapies,and chronotherapy.These furnish a theoretical foundation for formulating individualized treatment plans to achieve precise treatment for patients with OSCC.展开更多
调查瓜列当对新疆甜瓜的危害,探索瓜列当化学防控方法,为瓜列当防治措施制定及实施提供技术支撑。以新疆伽师县4个甜瓜主产乡镇为代表,调查瓜列当对甜瓜的危害程度及规律。结合新疆甜瓜栽培模式,进行2种除草剂各2种浓度对瓜列当的防治...调查瓜列当对新疆甜瓜的危害,探索瓜列当化学防控方法,为瓜列当防治措施制定及实施提供技术支撑。以新疆伽师县4个甜瓜主产乡镇为代表,调查瓜列当对甜瓜的危害程度及规律。结合新疆甜瓜栽培模式,进行2种除草剂各2种浓度对瓜列当的防治试验。调查的4个乡镇瓜列当都有不同程度的发生。甜瓜播种后50 d直到收获,都有瓜列当寄生危害。75%磺酰磺隆和甲基咪草烟都可用于对甜瓜列当的防治,磺酰磺隆效果优于甲基咪草烟。结合浇水冲施37.5 g a.i.·hm-275%磺酰磺隆,是最有效的新疆甜瓜瓜列当化学防治的施用方式和浓度。展开更多
[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tiss...[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tissue segregation and purification cultivation. Subsequently, using PDA medium plate culture method, the effect of various culture conditions on mycelium growth and sporulation of ginseng B. cinerea was detected. [ Result] The optimum temperature for mycelium growth and sporulation of B. cinerea was 25 ℃. The appropriate temperature for conidia germination ranged from 20 to 25 ℃. And the optimum pH value for mycelium growth and conidia germination was 6.0. The optimum carbon source was sucrose, followed by glucose and fructose. The optimum nitrogen source was peptone, fol- lowed by beef extract, yeast extract, alanine and ammonium nitrate. Among the media, the growth of mycelium cultured on PDA medium was the fastest with the production of gray mycelium and dense colonies. Lethal temperatures for sclerotia, mycelium and conidia were 60, 55 and 50 ℃, respectively. [ Conclusion] The study provided the scientific basis for the research on the incidence law of B. cinerea and its control.展开更多
基金supported by the following funding:National Natural Science Foundations of China(82002888,82272899 and 82370974)Sichuan Science and Technology Program(2022YFS0207 and 2023YFS0127)+1 种基金Scientific Research Foundation,WestChinaHospital of Stomatology SichuanUniversity(RCDWJS2021-8)the CAMS Innovation Fund for Medical Sciences(CIFMS,2019-I2M-5-004).
文摘This review explores the pivotal role of circadian rhythm regulators,particularly the PER genes,in Oral Squamous Cell Carcinoma(OSCC).As key constituents of the biological clock,PERs exhibit a downregulated expression pattern in OSCC,and the expression levels of PERs in OSCC patients are correlated with a favorable prognosis.PERs impact the occurrence and development of OSCC through multiple pathways.In the regulation of cell proliferation,they can function not only through cell cycle regultion but also via metabolic pathways.For example,PER1 can interact with receptors for activated C kinase 1(RACK1)and phosphatidylinositol 3-kinase(PI3K)through its PAS domain to inhibit glycolysis and thereby reduce cell proliferation.Regarding the regulation of cell death,PERs mediate various types of cell death in OSCC cells,such as p53-dependent apoptosis,protein kinase B(AKT)/mammalian target of rapamycin(mTOR)dependent autophagy,or hypoxia-inducible factor l-alpha(HIF-1a)mediated ferroptosis.In regulating epithelia-mesenchymal transition(EMT),PERs can lead to the downregulation of EMT related genes,such as zinc finger E-box binding homeobox 1/2(ZEBI/2),twist family BHLH transcription factor 1/2(TWIST1/2),and Vimentin,thereby influencing the migration and invasion capabilities of OSCC cells.In tumor angiogenesis,PERs exert regulatory effects on related factors,such as methionyl aminopeptidase 2(MetAP2)and vascular endothelial growth factor(VEGF).In the tumor immune microenvironment,PERs can inhibit the inhibitor of kappa B kinase(IKK)/nuclear factor kappa B(NF-kB)pathway and programmed cell death ligand 1(PD-L1)expression,thereby enhancing the cytotoxic effect of CD8+T cells on OSCC cells.In-depth studies focusing on elucidating the precise regulatory mechanisms of PERs can facilitate the development of therapeutic strategies targeting PERs,including restoration of PERs expression/activity,targeting PERs-regulated pathways,combination therapies,and chronotherapy.These furnish a theoretical foundation for formulating individualized treatment plans to achieve precise treatment for patients with OSCC.
文摘调查瓜列当对新疆甜瓜的危害,探索瓜列当化学防控方法,为瓜列当防治措施制定及实施提供技术支撑。以新疆伽师县4个甜瓜主产乡镇为代表,调查瓜列当对甜瓜的危害程度及规律。结合新疆甜瓜栽培模式,进行2种除草剂各2种浓度对瓜列当的防治试验。调查的4个乡镇瓜列当都有不同程度的发生。甜瓜播种后50 d直到收获,都有瓜列当寄生危害。75%磺酰磺隆和甲基咪草烟都可用于对甜瓜列当的防治,磺酰磺隆效果优于甲基咪草烟。结合浇水冲施37.5 g a.i.·hm-275%磺酰磺隆,是最有效的新疆甜瓜瓜列当化学防治的施用方式和浓度。
基金Supported by State Foreign Experts Bureau Projects(SFEBPS2005#0023)Technology Development Plan Project in Yanbian University(200802)~~
文摘[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tissue segregation and purification cultivation. Subsequently, using PDA medium plate culture method, the effect of various culture conditions on mycelium growth and sporulation of ginseng B. cinerea was detected. [ Result] The optimum temperature for mycelium growth and sporulation of B. cinerea was 25 ℃. The appropriate temperature for conidia germination ranged from 20 to 25 ℃. And the optimum pH value for mycelium growth and conidia germination was 6.0. The optimum carbon source was sucrose, followed by glucose and fructose. The optimum nitrogen source was peptone, fol- lowed by beef extract, yeast extract, alanine and ammonium nitrate. Among the media, the growth of mycelium cultured on PDA medium was the fastest with the production of gray mycelium and dense colonies. Lethal temperatures for sclerotia, mycelium and conidia were 60, 55 and 50 ℃, respectively. [ Conclusion] The study provided the scientific basis for the research on the incidence law of B. cinerea and its control.
