Complement C3 plays a critical role in periodontitis.However,its source,role and underlying mechanisms remain unclear.In our study,by analyzing single-cell sequencing data from mouse model of periodontitis,we identifi...Complement C3 plays a critical role in periodontitis.However,its source,role and underlying mechanisms remain unclear.In our study,by analyzing single-cell sequencing data from mouse model of periodontitis,we identified that C3 is primarily derived from periodontal fibroblasts.Subsequently,we demonstrated that C3a has a detrimental effect in ligature-induced periodontitis.C3ar−/−mice exhibited significantly less destruction of periodontal support tissues compared to wild-type mice,characterized by mild gingival tissue damage and reduced alveolar bone loss.This reduction was associated with decreased production of proinflammatory mediators and reduced osteoclast infiltration in the periodontal tissues.Mechanistic studies suggested that C3a could promote macrophage polarization and osteoclast differentiation.Finally,by analyzing single-cell sequencing data from the periodontal tissues of patients with periodontitis,we found that the results observed in mice were consistent with human data.Therefore,our findings clearly demonstrate the destructive role of fibroblast-derived C3 in ligature-induced periodontitis,driven by macrophage M1 polarization and osteoclast differentiation.These data strongly support the feasibility of C3a-targeted interventions for the treatment of human periodontitis.展开更多
Optimal health during pregnancy is crucial for ensuring the well-being of the mother and the developing fetus.This article is focused on the impact of oral health and the role of personalized oral hygiene management i...Optimal health during pregnancy is crucial for ensuring the well-being of the mother and the developing fetus.This article is focused on the impact of oral health and the role of personalized oral hygiene management in addressing prevalent dental issues among pregnant women,with particular emphasis on periodontal disease and dental caries.Despite the high prevalence of these dental problems and their association with obstetric complications such as pre-term birth and low birth weight,many pregnant women do not receive adequate dental care.This gap in care is often due to misconceptions about the safety of dental treat-ments during pregnancy and lack of awareness on the part of healthcare profes-sionals.Appreciations of the impacts of oral health and personalization of oral hygiene strategies such as tailored education and support,have proven effective in improving oral health in this population.Significant reductions in the incidence of caries and periodontal disease may be achieved by adapting care to the specific needs of each patient,thereby enhancing maternal and fetal health outcomes.Integration of personalized oral hygiene management into maternal health pro-grams and enhancement of ongoing education for pregnant women and healthcare professionals are essential steps in the reduction of pregnancy-related risks and improvement of maternal and neonatal well-being.Core Tip:In this article,we reviewed a recent study on the effects of personalized oral hygiene management on the oral health of pregnant women,as discussed in the article by Men et al.The study demonstrated that personalized oral hygiene interventions significantly improved oral health outcomes during pregnancy by reducing the prevalence of dental caries and periodontal disease.We emphasized the importance of individualized oral care programs that integrate education and tailored support,and we highlighted their significance in enhancing maternal and fetal health.This approach underscores the need for incorporating personalized oral hygiene management into routine prenatal care in order to optimize health outcomes.CONCLUSION This article emphasizes the critical role of personalized oral hygiene management in improving oral health during pregnancy.By tailoring oral care strategies to individual needs,significant improvements in dental health may be achieved,as evidenced by the reduced CAT scores observed in the experimental group in the study by Men et al[25].This personalized approach not only addresses common oral issues such as dental caries and periodontal disease but also underscores the broader implications for maternal and fetal health.Despite the positive results,there remains a gap in consistency in the application of oral health practices during pregnancy,partly due to misconceptions and lack of awareness among patients and healthcare providers.Future research should aim at validating these findings across diverse populations,investigating the impact of oral hygiene interventions at various stages of pregnancy,and evaluating their long-term effects on maternal and fetal health.Integrating personalized oral hygiene management into maternal health programs and promoting continuous education for pregnant women and healthcare professionals are essential steps toward enhancing overall health outcomes.By proactively managing oral health,the risks associated with pregnancy may be reduced while improving maternal and neonatal well-being.展开更多
BACKGROUND Periodontitis,when exacerbated by diabetes,is characterized by increased M1 macrophage polarization and decreased M2 polarization.O-linkedβ-N-acetylglucosamine(O-GlcNAcylation),catalyzed by O-GlcNAc transf...BACKGROUND Periodontitis,when exacerbated by diabetes,is characterized by increased M1 macrophage polarization and decreased M2 polarization.O-linkedβ-N-acetylglucosamine(O-GlcNAcylation),catalyzed by O-GlcNAc transferase(OGT),promotes inflammatory responses in diabetic periodontitis(DP).Additionally,p38 mitogen-activated protein kinase regulates macrophage polarization.However,the interplay between OGT,macrophage polarization,and p38 signaling in the progression of DP remains unexplored.AIM To investigate the effect of OGT on macrophage polarization in DP and its role in mediating O-GlcNAcylation of p38.METHODS For in vivo experiments,mice were divided into four groups:Control,DP model,model+short hairpin(sh)RNAnegative control,and model+sh-OGT.Diabetes was induced by streptozotocin,followed by ligation and lipopolysaccharide(LPS)administration to induce periodontitis.The impact of OGT was assessed by injecting sh-OGT lentivirus.Maxillary bone destruction was evaluated using micro-computed tomography analysis and tartrateresistant acid phosphatase staining,while macrophage polarization was determined through quantitative real-time polymerase chain reaction(qPCR)and immunohistochemistry.For in vitro experiments,RAW264.7 cells were treated with LPS and high glucose(HG)(25 mmol/L D-glucose)to establish a cell model of DP.OGT was inhibited by OGT inhibitor(OSMI4)treatment and knocked down by sh-OGT transfection.M1/M2 polarization was analyzed using qPCR,immunofluorescence,and flow cytometry.Levels of O-GlcNAcylation were measured using immunoprecipitation and western blotting.RESULTS Our results demonstrated that M1 macrophage polarization led to maxillary bone loss in DP mice,associated with elevated O-GlcNAcylation and OGT levels.Knockdown of OGT promoted the shift from M1 to M2 macrophage polarization in both mouse periodontal tissues and LPS+HG-induced RAW264.7 cells.Furthermore,LPS+HG enhanced the O-GlcNAcylation of p38 in RAW264.7 cells.OGT interacted with p38 to promote its O-GlcNAcylation at residues A28,T241,and T347,as well as its phosphorylation at residue Y221.CONCLUSION Inhibition of OGT-mediated p38 O-GlcNAcylation deactivates the p38 pathway by suppressing its self-phosphorylation,thereby promoting M1 to M2 macrophage polarization and mitigating DP.These findings suggested that modulating macrophage polarization through regulation of O-GlcNAcylation may represent a novel therapeutic strategy for treating DP.展开更多
BACKGROUND Lower salivary uric acid concentrations,the most abundant antioxidant agent in saliva,have been observed in patients with periodontitis compared to individuals with periodontal health.However,the independen...BACKGROUND Lower salivary uric acid concentrations,the most abundant antioxidant agent in saliva,have been observed in patients with periodontitis compared to individuals with periodontal health.However,the independent association of salivary uric acid levels with periodontitis,accounting for other risk factors,as well as its association with periodontitis severity,has not been investigated.AIM To the independent association of salivary uric acid levels with periodontitis and the association of salivary uric acid levels with the severity of periodontitis.METHODS This observational and prospective study measured salivary uric acid levels in subjects with periodontitis(characterized by periodontal loss of tissue)and in subjects without periodontitis(periodontal health or localized gingivitis in<30%of sites).Multivariate regression analysis was performed to determine independent factors associated with periodontitis.Spearman’s rho correlation coefficient was used to assess the relationship between salivary uric acid levels and periodontitis severity.A receiver operating characteristic analysis was carried out to evaluate the diagnostic performance of salivary uric acid levels in periodontitis,reporting the area under curve(AUC)and its 95%CI.RESULTS We included 121 subjects,61 of them with periodontitis and 60 without periodontitis(39 with periodontal health and 21 with local gingivitis).Subjects with periodontitis compared to those without periodontitis were older(P<0.001),had higher salivary uric acid levels(P=0.002),higher rate of arterial hypertension history(P=0.001)and higher rate of never-smoker history(P<0.001).The AUC for periodontitis diagnosis by salivary uric acid levels was 66%(95%CI:57%-75%;P<0.001)and the better cut-off point was 111 nmol/mL.Multiple logistic regression analysis showed an independent association of salivary uric acid levels<111 nmol/mL(OR=6.14;95%CI:2.015-18.721;P=0.001)with periodontitis after controlling for age,never-smoker history and arterial hypertension.A negative correlation of salivary uric acid levels and periodontitis severity was observed(rho=-0.32;P<0.001).CONCLUSION The two novel findings of our research were,first,that low salivary uric acid concentrations are independently associated with periodontitis,even after accounting for established risk factors.Second,salivary uric acid levels show a negative correlation with periodontitis severity.展开更多
BACKGROUND Dental follicle stem cell(DFSC)sheets demonstrate strong extracellular secretion capabilities and efficacy in periodontal regeneration.However,existing methods for producing DFSC sheets lack a comprehensive...BACKGROUND Dental follicle stem cell(DFSC)sheets demonstrate strong extracellular secretion capabilities and efficacy in periodontal regeneration.However,existing methods for producing DFSC sheets lack a comprehensive discussion on the most efficient and cost-effective approaches at the good manufacturing practice(GMP)level.AIM To investigate the culture condition of GMP-compliant DFSC sheets and to compare the properties of DFSC sheets and cell suspensions.METHODS This study explored the optimal conditions for culturing GMP-compliant DFSC sheets,focusing on four key factors:Cell passage,cell concentration,L-ascorbic acid content,and culture duration.We evaluated the characteristics of the cell sheets under varying culture conditions,including cell viability,cell count,appearance,osteogenesis,chondrogenesis,odontogenesis,aging,relative telomere length,and extracellular matrix secretion.A comparison was also made between the periodontal regeneration,osteogenesis,and paracrine capacity of cell sheets cultured under optimal conditions and those of the cell suspensions.RESULTS The GMP-compliant DFSC sheets cultured from passage 4 cells exhibited the highest viability(≥99%,P<0.05)and optimal osteogenic differentiation capacity(optical density≥0.126,P<0.05).When cultured for 10 days,DFSC sheets demonstrated maximal expression of osteogenic,chondrogenic and periostin genes[alkaline phosphatase,Runt-related transcription factor 2,collagen type I,osteopontin,cartilage associated protein,and PERIOSTN(P<0.001);osteocalcin(P<0.01)].Concurrently,they showed the lowest senescent cell count(P<0.01)with no progression to late-stage senescence.At a seeding density of 2500 cells/cm^(2),GMP-compliant DFSC sheets achieved better osteogenic differentiation(P<0.01)and maximal osteogenic,chondrogenic and periostin gene expression(P<0.001),coupled with the highest hydroxyproline secretion(P<0.001)and moderate sulfated glycosaminoglycan production.No statistically significant difference in senescent cell count was observed compared to DFSC sheets at a seeding density of 5000 cells/cm^(2).Supplementation with 25μg/mL L-ascorbic acid significantly enhanced osteogenic gene expression(P<0.001)and elevated hydroxyproline(P<0.01)and sulfated glycosaminoglycan secretion to high ranges.Compared with the cell suspension,the cell sheet demonstrated improved osteogenic,paracrine,and periodontal regenerative capacities in Sprague-Dawley rats.The optimized DFSC sheets demonstrated significantly higher levels of vascular endothelial growth factor and angiopoietin-1(P<0.001)compared to DFSC suspensions,along with enhanced osteogenic induction outcomes(optical density=0.1333±0.01270 vs 0.1007±0.0005774 in suspensions,P<0.05).Following implantation into the rat periodontal defect model,micro-computed tomography analysis revealed superior bone regeneration metrics in the cell sheet group compared to both the cell suspension group and control group(percent bone volume,trabecular thickness,trabecular number),while trabecular spacing exhibited an inverse pattern.CONCLUSION Optimized DFSC sheets cultured under the identified conditions outperform DFSC suspensions.This study contributes to the industrial-scale production of DFSC sheets and establishes a foundation for cell therapy applications.展开更多
Periodontitis has emerged as one of the most critical oral diseases, and research on this condition holds great importance for the advancement of stomatology. As the most authoritative national scientific research fun...Periodontitis has emerged as one of the most critical oral diseases, and research on this condition holds great importance for the advancement of stomatology. As the most authoritative national scientific research funding institution in China, the National Natural Science Foundation of China (NSFC) has played a pivotal role in driving the progress of periodontal science by supporting research on periodontitis. This article provides a comprehensive review of the research and development progress related to periodontitis in China from 2014 to 2023, highlighting the significant contributions of the NSFC to this field. We have summarized the detailed funding information from the NSFC, including the number of applicant codes, funded programs and the distribution of funded scholars. These data illustrate the efforts of the NSFC in cultivating young scientists and building research groups to address key challenges in national scientific research. This study offers an overview of the current hot topics, recent breakthroughs and future research prospects related to periodontitis in China.展开更多
Inflammation represents a critical immune response triggered by cellular activities and inflammatory mediators following tissue damage.It plays a central role in the pathological progression of diverse diseases,includ...Inflammation represents a critical immune response triggered by cellular activities and inflammatory mediators following tissue damage.It plays a central role in the pathological progression of diverse diseases,including psychiatric disorders,cancer,and immunological conditions,rendering it an essential target for therapeutic intervention.Periodontitis,a prevalent oral inflammatory disease,is a leading cause of tooth loss and poses significant health challenges globally.Traditionally,inflammatory diseases such as periodontitis have been treated with systemic administration of synthetic chemicals.However,recent years have witnessed challenges,including drug resistance and microbial dysbiosis associated with these treatments.In contrast,natural products derived from Chinese medicine offer numerous benefits,such as high safety profiles,minimal side effects,innovative pharmacological mechanisms,ease of extraction,and multiple targets,rendering them viable alternatives to conventional antibiotics for treating inflammatory conditions.Numerous effective anti-inflammatory natural products have been identified in traditional Chinese medicine(TCM),including alkaloids,flavonoids,terpenoids,lignans,and other natural products that exhibit inhibitory effects on inflammation and are potential therapeutic agents.Several studies have confirmed the substantial anti-inflammatory and immunomodulatory properties of these compounds.This comprehensive review examines the literature on the anti-inflammatory effects of TCM-derived natural products from databases such as PubMed,Web of Science,and CNKI,focusing on terms like"inflammation","periodontitis","pharmacology",and"traditional Chinese medicine".The analysis systematically summarizes the molecular pharmacology,chemical composition,and biological activities of these compounds in inflammatory responses,alongside their mechanisms of action.This research seeks to deepen understanding of the mechanisms and biological activities of herbal extracts in managing inflammatory diseases,potentially leading to the development of promising new anti-inflammatory drug candidates.Future applications could extend to the treatment of various inflammatory conditions,including periodontitis.展开更多
The prevalence of cardiometabolic syndrome(CMS)and increasing mortality rate play a significant role in the global increase of cardiovascular disease(CVD)in developing countries.A group of metabolic syndromes that are...The prevalence of cardiometabolic syndrome(CMS)and increasing mortality rate play a significant role in the global increase of cardiovascular disease(CVD)in developing countries.A group of metabolic syndromes that are risk factors for CVDs are referred to as the CMS.Although the exact mechanism(s)behind the development of the CMS are not known,but multi-organ insulin resistance,a prevalent characteristic of the syndrome,is probably one of them.The two most prevalent dental diseases i.e.periodontitis(PD)and dental caries have been re-lated to several systemic diseases and disorders,such as CMS.Age,alcohol con-sumption,being obese,possessing diabetes,as well as smoking are risk factors for periodontal diseases,while both CVD and periodontal diseases are linked to systemic inflammation.It has a multifactorial aetiology and is associated with many systemic diseases.When bacteria and their products attack the periodontal tissues,the tissue raises an immune-inflammatory response against the patho-gens.This acute phase response is a result of the pathogen’s systemic attack and contributes to the overall inflammatory burden of the system.CVD and PD are both diseases associated with systemic inflammation and may be related as they share many common risk factors.Hence,the correlation between these conditions might also have an impact on how dentistry and medicine are practised,thus helping to build a working relationship between the dentist and the physician.展开更多
In the fight against bacterial infections,it is critical to effectively disrupt biofilms.However,disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents.Herein,we prese...In the fight against bacterial infections,it is critical to effectively disrupt biofilms.However,disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents.Herein,we present a self-propelled nanovesicle(PCL-PLG@CHX)strategy for eliminating biofilms and further expediting the healing of wounds.PCL-PLG@CHX is synthesized by assembling vesicles from amphiphilic polymers,which incorporate both poly-ε-caprolactone and guanidinated-poly-ε-lysine(PCL-PLG)and are infused with chlorhexidine(CHX).Upon application to sites of bacterial infection,PCL-PLG@CHX,abundant in guanidinium structures,effectively accumulates on the negatively charged surface of biofilms.It interacts with reactive oxygen species(ROS)within the biofilm,leading to nitric oxide(NO)production.The generated NO cannot only propel the nanovesicle to penetrate deeper into the biofilm,but also act as a signaling molecule to disperse the biofilm,working in conjunction with the subsequent release of CHX for an enhanced antibacterial impact.Following the eradication of bacteria,the residual guanidine component continues to produce small quantities of NO,facilitating angiogenesis and epithelial growth,thereby accelerating the healing of wounds.Together,our study shows that PCL-PLG@CHX utilizes the potential of guanidine moieties to efficiently break down biofilms and support tissue restoration,tackling the pivotal challenge of biofilm-related diseases.展开更多
BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves ...BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves mechanical debridement and plaque control,but localized antimicrobial therapy offers site-specific advantages.While antibiotics such as metronidazole and doxycycline are commonly used,green tea extract,which is rich in epigallocatechin gallate,has been proposed as a promising alternative for local drug delivery due to its anti-inflammatory and antimicrobial properties.AIM To compare the clinical efficacy of green tea extract gel and ornidazole gel as adjuncts to scaling and root planing(SRP)in patients with periodontitis.METHODS Teeth with probing pocket depths(PPD)of 4-7 mm were selected.Participants’baseline oral hygiene index-simplified,plaque index,clinical attachment loss,and PPD were recorded.The participants were randomized into two groups:One received green tea extract gel after SRP,and the other received ornidazole gel.Subgingival drug delivery was performed,and participants refrained from brushing or interproximal cleaning for ten days.Their clinical parameters were re-evaluated after one month.RESULTS The PPD decreased significantly from baseline to one month in both groups.However,the green tea extract gel group exhibited superior outcomes to the ornidazole gel group,with a mean difference in PPD of 0.28±0.78 mm at one month(P<0.007).CONCLUSION As an adjunct to SRP,green tea extract gel showed greater efficacy in improving clinical periodontal parameters than ornidazole gel.展开更多
OBJECTIVE:To investigate the effect and mechanism of Sanqi(Radix Notoginseng)in treating periodontitis.METHODS:The active components and periodontitis targets were analyzed through network pharmacology and molecular d...OBJECTIVE:To investigate the effect and mechanism of Sanqi(Radix Notoginseng)in treating periodontitis.METHODS:The active components and periodontitis targets were analyzed through network pharmacology and molecular docking.A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi(Radix Notoginseng)at different doses for 30 d.The alveolar bone structure was observed by micro-CT,the periodontal tissue structure was observed by hematoxylin-eosin staining,and the related proteins changes was detected by immunohistochemical staining.RESULTS:Sanqi(Radix Notoginseng)and periodontitis had a total of 96 coincident targets that were significantly enriched in the interleukin 17(IL-17),tumor necrosis factor(TNF),and advanced glycation endproducts and the receptor of advanced glycation endproducts signaling pathways.The active compound quercetin had good binding activity with interleukin 6(IL-6),vascular endothelial growth factor A(VEGFA),matrix metallopeptidase 9(MMP9),tumor necrosis factorα(TNF-α),Jun proto-oncogene(JUN),and C-X-C motif chemokine ligand 8(CXCL8)in periodontitis.Compared with normal group,the distance from the cementoenamel junction(CEJ)to the alveolar bone(AB)was increased,alveolar bone absorption was obvious,the periodontal tissue structure was disorganized,and IL-6 and TNF-αwere upregulated in periodontitis group;meanwhile,the distance from CEJ to AB was significantly decreased,alveolar bone resorption was reduced,periodontal tissue structure was improved,the expression of IL-6,TNF-α,IL-17 and retinoid-ralated orphan receptorγt(RORγt)were decreased,Forkhead Box P3(FOXP3)and IL-10 were increased after Sanqi(Radix Notoginseng)treatment.CONCLUSIONS:Sanqi(Radix Notoginseng)improves the structure of alveolar bone and gum,and reduces inflammation;the mechanism involve in inhibiting IL-17 signaling pathway to suppress Th17 and promote Treg cells differentiation.展开更多
This article explores the association between salivary uric acid(UA)and periodontitis,systematically analyzing its dual roles and research progress.Studies indicate that UA acts as a primary antioxidant in saliva unde...This article explores the association between salivary uric acid(UA)and periodontitis,systematically analyzing its dual roles and research progress.Studies indicate that UA acts as a primary antioxidant in saliva under physiological conditions(accounting for 70%),protecting periodontal tissues by scavenging reactive oxygen species.However,when gum disease becomes severe,UA can switch roles and fuel inflammation,worsening tissue damage.Lorente et al’s research found an independent inverse correlation between salivary UA levels and periodontitis severity(odds ratio=6.14,P=0.001),establishing 111 nmol/mL as a diagnostic threshold(area under the curve=66%).Nevertheless,limitations include sample heterogeneity and failure to distinguish between gingivitis and periodontitis.Mechanistically,three hypotheses are proposed:The Antioxidant Depletion Hypothesis(UA oxidation consumption leading to feedback loops),the Microbial Metabolic Hijacking Hypothesis(pathogens utilizing UA as a carbon source to disrupt redox balance),and the Epithelial Barrier Dysfunction Hypothesis(UA deficiency causing downregulation of tight junction proteins).Future research should prioritize longitudinal cohorts to validate predictive value,integrate multi-omics to explore dysregulated signatures,and develop UA supplementation or targeted antioxidant therapies.This study provides novel insights into periodontitis diagnosis and mechanisms,advancing the application of salivary biomarkers in precision periodontics.展开更多
In this letter,we delve into the groundbreaking research by Lorente et al,which sheds light on the intricate relationship between low salivary uric acid levels and periodontitis.The study not only confirms previous ob...In this letter,we delve into the groundbreaking research by Lorente et al,which sheds light on the intricate relationship between low salivary uric acid levels and periodontitis.The study not only confirms previous observations of reduced salivary uric acid concentrations in periodontitis patients but also establishes,for the first time,an independent association between these two factors,even when controlling for traditional risk factors such as age,smoking status,and arterial hypertension.Moreover,the findings reveal a significant negative correlation between salivary uric acid levels and the severity of periodontitis,suggesting that this biomarker may serve as a valuable indicator of disease progression.These discoveries open new avenues for understanding the pathophysiology of period-ontitis and pave the way for innovative diagnostic and therapeutic strategies.The potential clinical applications of salivary uric acid measurement,such as guiding personalized treatment plans and monitoring disease activity,warrant further exploration to enhance patient care and improve outcomes in this prevalent inflammatory condition.展开更多
Rheumatoid arthritis(RA),an autoimmune disease characterized by chronic inflammation of synovial tissue,is divided into two subtypes-anti-citrullinated protein antibody(ACPA)-positive and ACPA-negative RA.While the pa...Rheumatoid arthritis(RA),an autoimmune disease characterized by chronic inflammation of synovial tissue,is divided into two subtypes-anti-citrullinated protein antibody(ACPA)-positive and ACPA-negative RA.While the pathogenic mechanisms of ACPA-positive RA are well-understood,the etiology of ACPA-negative RA remains largely unknown.The association between RA and periodontitis(PD)has been observed since the early 190os,with the two diseases sharing common genetic and environmental risk factors that lead to the progressive destruction of bone and connective tissue.However,the associations between PD and the two subtypes of RA differ.This comprehensive review aims to provide an updated understanding of the epidemiological association between RA and PD,explore potential pathogenic mechanisms linking the two diseases,and highlight the key distinctions between the subtypes of RA and their respective associations with PD.We also discuss the possibility of early intervention or the treatment of the two diseases.Ultimately,this review aims to provide valuable insights for future research in this field.展开更多
Objective To investigate the treatment effect of the histone demethylase inhibitor GSK-J4,a small molecule that inhibits the demethylase activity of Jumonji domain-containing protein 3(JMJD3),in the treatment of perio...Objective To investigate the treatment effect of the histone demethylase inhibitor GSK-J4,a small molecule that inhibits the demethylase activity of Jumonji domain-containing protein 3(JMJD3),in the treatment of periodontitis.Methods Gingival tissues from patients with moderate to severe chronic periodontitis and healthy controls were collected to evaluate JMJD3 expression via real-time quantitative reverse transcription PCR(RT-qPCR)and immunohistochemistry(IHC).Next,Sprague–Dawley(SD)rats were used to investigate the effect of GSK-J4 in vivo.The experimental periodontitis model was induced by upper first molar ligation and gingival sulcus injection of Porphyromonas gingivalis.The rats were divided into a healthy group,a periodontitis group,periodontitis plus GSK-J4 treatment groups(P+GSK-J415 mg/kg or 25 mg/kg),and a periodontitis plus dimethyl sulfoxide(DMSO)group(P+DMSO).After 4 weeks,maxillary molar segments were assessed via micro-computed tomography(CT)and hematoxylin and eosin(HE)staining.Serum tumor necrosis factor-α(TNF-α)levels were measured by enzyme-linked immunosorbent assay(ELISA).Results Higher expression of the Jmjd3 gene and JMJD3 protein was detected in human inflamed gingiva than in healthy gingiva(P<0.05).GSK-J4 administration reversed alveolar bone absorption[i.e.,reduced alveolar bone crest(ABC)-cementoenamel junction(CEJ)distance],reduced inflammatory cell accumulation at the crest of the alveolar bone,and alleviated serum TNF-αlevels in rats with periodontitis.Moreover,the number of H3K27me3-positive nuclei was greater in model rats treated with GSK J4 than in model rats.Conclusions The histone demethylase inhibitor GSK-J4 attenuated periodontal bone loss and inflammation in a rat periodontitis model by targeting JMJD3.展开更多
Periodontitis is an inflammatory infectious disease affecting the periodontal supporting tissues and is the primary cause of tooth loosening and tooth loss in adults.Clinically,supragingival scaling,subgingival scalin...Periodontitis is an inflammatory infectious disease affecting the periodontal supporting tissues and is the primary cause of tooth loosening and tooth loss in adults.Clinically,supragingival scaling,subgingival scaling,root planing,and other basic periodontal treatments,often combined with antibiotic therapy,are commonly employed with moderate efficacy.However,the increasing prevalence of antibiotic resistance and associated adverse reactions has become a growing concern.Recent studies have demonstrated the significant impact of traditional Chinese medicine(TCM)extracts in both the prevention and treatment of periodontitis,exhibiting remarkable effectiveness.This review explores the role and mechanisms of TCM extracts in the prevention and treatment of periodontitis,providing a reference for further elucidation of their mechanisms and a theoretical basis for the development of Chinese herbal medicine-based care products.展开更多
BACKGROUND Periodontitis is a chronic inflammatory disorder influenced by both behavioral and genetic factors.Among epigenetic regulators,the ANRIL gene has been proposed as a risk factor for periodontitis;however,fin...BACKGROUND Periodontitis is a chronic inflammatory disorder influenced by both behavioral and genetic factors.Among epigenetic regulators,the ANRIL gene has been proposed as a risk factor for periodontitis;however,findings on the association between ANRIL polymorphisms and disease susceptibility remain inconsistent.AIM To analyze the association between the rs1333048 genetic polymorphism in the ANRIL gene and periodontitis via meta-analysis.METHODS A literature search was performed for studies published before May 2,2025.The Review Manager statistical program was used in analyses with calculations of heterogeneity index(I^(2))and odds ratio(OR)with 95%of confidence intervals(CI).Begg’s test and the Egger’s linear regression test were used for publication bias evaluation using Comprehensive meta-analysis software.P<0.05 was considered significant.da Silva FRP et al.ANRIL gene and periodontitis:A meta-analysis WJMA https://www.wjgnet.com 2 December 18,2025 Volume 13 Issue 4 RESULTS From 12 studies including 5489 participants across multiple ethnic groups,we observed a statistically significant association between ANRIL gene polymorphisms and periodontitis in the allelic contrast model(OR=1.2495%CI:1.15-1.34,P<0.00001).Conversely,the wild-type allele was significantly associated with the control group(OR=0.8095%CI:0.75-0.87,P<0.00001).Heterogeneity was low(I²=28%,Pheterogeneity=0.17),and no significant risk of publication bias was detected(P>0.05).CONCLUSION In conclusion,this meta-analysis demonstrated a significant association between the rs1333048 polymorphism and periodontitis in the overall analysis and in stratified analyses of Caucasian populations,but in for mixed-race populations.展开更多
Periodontitis is a chronic inflammatory disease caused by oral pathogens,and the osteogenic potential of human periodontal ligament stem cells(hPDLSCs)is severely impaired under the inflammatory environment.Current cl...Periodontitis is a chronic inflammatory disease caused by oral pathogens,and the osteogenic potential of human periodontal ligament stem cells(hPDLSCs)is severely impaired under the inflammatory environment.Current clinical periodontitis treatment strategies such as surgical interventions and antibiotic delivery still suffer from poor antibacterial efficacy,difficulty in ameliorating excessive inflammatory responses and slow periodontal tissue regeneration.Here,we have innovatively developed a non-surgical treatment strategy based on a functional composite hydrogel.A composite hydrogel system(Pt@ZIF-8/ALN-ac/Gel)containing bioactive zeolite imidazolate framework-8(ZIF-8)integrated with platinum nanoparticles(Pt@ZIF-8)and alendronate acrylamide(ALN-ac)was constructed on the basis of gelatin methacryloyl(GelMA)to achieve enhanced antibacterial effect and reactive oxygen species(ROS)scavenging ability while promoting the osteogenic potential of hPDLSCs.We confirmed that Pt@ZIF-8/ALN-ac/Gel was able to continuously release Zn^(2+)and exerted an obvious antibacterial effect against Porphyromonas gingivalis.In vitro experiments proved that Pt@ZIF-8/ALN-ac/Gel had good biocompatibility,while efficiently featuring excellent reactive oxygen species(ROS)scavenging capacity,increasing alkaline phosphatase activity,and promoting extracellular matrix mineralization by hPDLSCs.In vivo,Pt@ZIF-8/ALN-ac/Gel significantly inhibited the alveolar bone deterioration and reduced osteoclast activation and inflammation,thereby promoting the regeneration of damaged tissues.These findings demonstrated superior therapeutic efficacy in the reported clinical periodontitis treatment,exhibiting great potential for application.展开更多
Periodontitis is the commonest oral disease affecting population worldwide. This disease is notorious for the devastation of tooth supporting structures, ensuing in the loss of dentition. The etiology for this disease...Periodontitis is the commonest oral disease affecting population worldwide. This disease is notorious for the devastation of tooth supporting structures, ensuing in the loss of dentition. The etiology for this disease is bacterial biofilm, which accumulates on the teeth as dental plaque. In addition to the biofilm microorganisms, other factors such as environmental, systemic and genetic are also responsible in progression of periodontitis. Diabetes mellitus (DM) is metabolic disorder which has an impact on the global health. DM plays a crucial role in the pathogenesis of periodontitis. Periodontitis is declared as the “sixth” major complication of DM. Evidence based literature has depicted an enhanced incidence and severity of periodontitis in subjects with DM. A “two way” relationship has been purported between periodontitis and DM. Mutual management of both conditions is necessary. Periodontal therapy (PT) may assist to diminish the progression of DM and improve glycemic control. Various advanced technological facilities may be utilized for the purpose of patient education and disease management. The present paper clarifies the etio-pathogenesis of periodontitis, establishing it as a complication of DM and elaborating the various mechanisms involved in the pathogenesis. The role of PT in amelioration of DM and application of digital communication will be discussed. Overall, it is judicious to create an increased patient cognizance of the periodontitis-DM relationship. Conjunctive efforts must be undertaken by the medical and oral health care professionals for the management of periodontitis affected DM patients.展开更多
Objective: To discuss the expression of RUNX2 and MDM21 in rats with periodontitis under the chronic intermittent hypoxia. Methods: A total of 32 SD healthy rats were randomly divided into four groups, with 8 rats in ...Objective: To discuss the expression of RUNX2 and MDM21 in rats with periodontitis under the chronic intermittent hypoxia. Methods: A total of 32 SD healthy rats were randomly divided into four groups, with 8 rats in each group. The molecular biological techniques of immunohistochemistry, RT-PCR and Western blotting were employed to detect the effect of different hypoxia time(0, 6, 12, 24 and 48 h) and different concentrations of hypoxia(0.000, 0.001, 0.010, 0.060 and 0.100 ppm) on the expression of RUNX2 and MDM21 in rats of four groups. Results: The expression of RUNX2 and MDM21 in each group was significantly higher than the one at other concentrations when the concentration was 0.010 ppm, with the statistical difference(P<0.05). The expression of RUNX2 and MDM21 was that normoxic control group > periodontitis group> chronic intermittent hypoxia group > compound group under the action with the concentration of 0.010 ppm for 12 h, but there was no significant difference for the comparison among groups(P>0.05). Conclusions: The condition of chronic intermittent hypoxia can reduce the expression of RUNX2 and MDM21 in rats with periodontitis and aggravate the damage of periodontal bone.展开更多
基金supported by the National Key R&D Program of China(No.2022YFC2504200)the National Natural Science Foundation of China(Nos.82370936,81920108012,82471032).
文摘Complement C3 plays a critical role in periodontitis.However,its source,role and underlying mechanisms remain unclear.In our study,by analyzing single-cell sequencing data from mouse model of periodontitis,we identified that C3 is primarily derived from periodontal fibroblasts.Subsequently,we demonstrated that C3a has a detrimental effect in ligature-induced periodontitis.C3ar−/−mice exhibited significantly less destruction of periodontal support tissues compared to wild-type mice,characterized by mild gingival tissue damage and reduced alveolar bone loss.This reduction was associated with decreased production of proinflammatory mediators and reduced osteoclast infiltration in the periodontal tissues.Mechanistic studies suggested that C3a could promote macrophage polarization and osteoclast differentiation.Finally,by analyzing single-cell sequencing data from the periodontal tissues of patients with periodontitis,we found that the results observed in mice were consistent with human data.Therefore,our findings clearly demonstrate the destructive role of fibroblast-derived C3 in ligature-induced periodontitis,driven by macrophage M1 polarization and osteoclast differentiation.These data strongly support the feasibility of C3a-targeted interventions for the treatment of human periodontitis.
文摘Optimal health during pregnancy is crucial for ensuring the well-being of the mother and the developing fetus.This article is focused on the impact of oral health and the role of personalized oral hygiene management in addressing prevalent dental issues among pregnant women,with particular emphasis on periodontal disease and dental caries.Despite the high prevalence of these dental problems and their association with obstetric complications such as pre-term birth and low birth weight,many pregnant women do not receive adequate dental care.This gap in care is often due to misconceptions about the safety of dental treat-ments during pregnancy and lack of awareness on the part of healthcare profes-sionals.Appreciations of the impacts of oral health and personalization of oral hygiene strategies such as tailored education and support,have proven effective in improving oral health in this population.Significant reductions in the incidence of caries and periodontal disease may be achieved by adapting care to the specific needs of each patient,thereby enhancing maternal and fetal health outcomes.Integration of personalized oral hygiene management into maternal health pro-grams and enhancement of ongoing education for pregnant women and healthcare professionals are essential steps in the reduction of pregnancy-related risks and improvement of maternal and neonatal well-being.Core Tip:In this article,we reviewed a recent study on the effects of personalized oral hygiene management on the oral health of pregnant women,as discussed in the article by Men et al.The study demonstrated that personalized oral hygiene interventions significantly improved oral health outcomes during pregnancy by reducing the prevalence of dental caries and periodontal disease.We emphasized the importance of individualized oral care programs that integrate education and tailored support,and we highlighted their significance in enhancing maternal and fetal health.This approach underscores the need for incorporating personalized oral hygiene management into routine prenatal care in order to optimize health outcomes.CONCLUSION This article emphasizes the critical role of personalized oral hygiene management in improving oral health during pregnancy.By tailoring oral care strategies to individual needs,significant improvements in dental health may be achieved,as evidenced by the reduced CAT scores observed in the experimental group in the study by Men et al[25].This personalized approach not only addresses common oral issues such as dental caries and periodontal disease but also underscores the broader implications for maternal and fetal health.Despite the positive results,there remains a gap in consistency in the application of oral health practices during pregnancy,partly due to misconceptions and lack of awareness among patients and healthcare providers.Future research should aim at validating these findings across diverse populations,investigating the impact of oral hygiene interventions at various stages of pregnancy,and evaluating their long-term effects on maternal and fetal health.Integrating personalized oral hygiene management into maternal health programs and promoting continuous education for pregnant women and healthcare professionals are essential steps toward enhancing overall health outcomes.By proactively managing oral health,the risks associated with pregnancy may be reduced while improving maternal and neonatal well-being.
基金Supported by the National Natural Science Foundation of China,No.81973684Natural Science Foundation of Sichuan Province,No.2023NSFSC1760Youth Talent Fund of Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital,No.2021QN09。
文摘BACKGROUND Periodontitis,when exacerbated by diabetes,is characterized by increased M1 macrophage polarization and decreased M2 polarization.O-linkedβ-N-acetylglucosamine(O-GlcNAcylation),catalyzed by O-GlcNAc transferase(OGT),promotes inflammatory responses in diabetic periodontitis(DP).Additionally,p38 mitogen-activated protein kinase regulates macrophage polarization.However,the interplay between OGT,macrophage polarization,and p38 signaling in the progression of DP remains unexplored.AIM To investigate the effect of OGT on macrophage polarization in DP and its role in mediating O-GlcNAcylation of p38.METHODS For in vivo experiments,mice were divided into four groups:Control,DP model,model+short hairpin(sh)RNAnegative control,and model+sh-OGT.Diabetes was induced by streptozotocin,followed by ligation and lipopolysaccharide(LPS)administration to induce periodontitis.The impact of OGT was assessed by injecting sh-OGT lentivirus.Maxillary bone destruction was evaluated using micro-computed tomography analysis and tartrateresistant acid phosphatase staining,while macrophage polarization was determined through quantitative real-time polymerase chain reaction(qPCR)and immunohistochemistry.For in vitro experiments,RAW264.7 cells were treated with LPS and high glucose(HG)(25 mmol/L D-glucose)to establish a cell model of DP.OGT was inhibited by OGT inhibitor(OSMI4)treatment and knocked down by sh-OGT transfection.M1/M2 polarization was analyzed using qPCR,immunofluorescence,and flow cytometry.Levels of O-GlcNAcylation were measured using immunoprecipitation and western blotting.RESULTS Our results demonstrated that M1 macrophage polarization led to maxillary bone loss in DP mice,associated with elevated O-GlcNAcylation and OGT levels.Knockdown of OGT promoted the shift from M1 to M2 macrophage polarization in both mouse periodontal tissues and LPS+HG-induced RAW264.7 cells.Furthermore,LPS+HG enhanced the O-GlcNAcylation of p38 in RAW264.7 cells.OGT interacted with p38 to promote its O-GlcNAcylation at residues A28,T241,and T347,as well as its phosphorylation at residue Y221.CONCLUSION Inhibition of OGT-mediated p38 O-GlcNAcylation deactivates the p38 pathway by suppressing its self-phosphorylation,thereby promoting M1 to M2 macrophage polarization and mitigating DP.These findings suggested that modulating macrophage polarization through regulation of O-GlcNAcylation may represent a novel therapeutic strategy for treating DP.
文摘BACKGROUND Lower salivary uric acid concentrations,the most abundant antioxidant agent in saliva,have been observed in patients with periodontitis compared to individuals with periodontal health.However,the independent association of salivary uric acid levels with periodontitis,accounting for other risk factors,as well as its association with periodontitis severity,has not been investigated.AIM To the independent association of salivary uric acid levels with periodontitis and the association of salivary uric acid levels with the severity of periodontitis.METHODS This observational and prospective study measured salivary uric acid levels in subjects with periodontitis(characterized by periodontal loss of tissue)and in subjects without periodontitis(periodontal health or localized gingivitis in<30%of sites).Multivariate regression analysis was performed to determine independent factors associated with periodontitis.Spearman’s rho correlation coefficient was used to assess the relationship between salivary uric acid levels and periodontitis severity.A receiver operating characteristic analysis was carried out to evaluate the diagnostic performance of salivary uric acid levels in periodontitis,reporting the area under curve(AUC)and its 95%CI.RESULTS We included 121 subjects,61 of them with periodontitis and 60 without periodontitis(39 with periodontal health and 21 with local gingivitis).Subjects with periodontitis compared to those without periodontitis were older(P<0.001),had higher salivary uric acid levels(P=0.002),higher rate of arterial hypertension history(P=0.001)and higher rate of never-smoker history(P<0.001).The AUC for periodontitis diagnosis by salivary uric acid levels was 66%(95%CI:57%-75%;P<0.001)and the better cut-off point was 111 nmol/mL.Multiple logistic regression analysis showed an independent association of salivary uric acid levels<111 nmol/mL(OR=6.14;95%CI:2.015-18.721;P=0.001)with periodontitis after controlling for age,never-smoker history and arterial hypertension.A negative correlation of salivary uric acid levels and periodontitis severity was observed(rho=-0.32;P<0.001).CONCLUSION The two novel findings of our research were,first,that low salivary uric acid concentrations are independently associated with periodontitis,even after accounting for established risk factors.Second,salivary uric acid levels show a negative correlation with periodontitis severity.
基金Supported by National Key Research and Development Program of China,No.2021YFA1100600 and No.2022YFA1104400.
文摘BACKGROUND Dental follicle stem cell(DFSC)sheets demonstrate strong extracellular secretion capabilities and efficacy in periodontal regeneration.However,existing methods for producing DFSC sheets lack a comprehensive discussion on the most efficient and cost-effective approaches at the good manufacturing practice(GMP)level.AIM To investigate the culture condition of GMP-compliant DFSC sheets and to compare the properties of DFSC sheets and cell suspensions.METHODS This study explored the optimal conditions for culturing GMP-compliant DFSC sheets,focusing on four key factors:Cell passage,cell concentration,L-ascorbic acid content,and culture duration.We evaluated the characteristics of the cell sheets under varying culture conditions,including cell viability,cell count,appearance,osteogenesis,chondrogenesis,odontogenesis,aging,relative telomere length,and extracellular matrix secretion.A comparison was also made between the periodontal regeneration,osteogenesis,and paracrine capacity of cell sheets cultured under optimal conditions and those of the cell suspensions.RESULTS The GMP-compliant DFSC sheets cultured from passage 4 cells exhibited the highest viability(≥99%,P<0.05)and optimal osteogenic differentiation capacity(optical density≥0.126,P<0.05).When cultured for 10 days,DFSC sheets demonstrated maximal expression of osteogenic,chondrogenic and periostin genes[alkaline phosphatase,Runt-related transcription factor 2,collagen type I,osteopontin,cartilage associated protein,and PERIOSTN(P<0.001);osteocalcin(P<0.01)].Concurrently,they showed the lowest senescent cell count(P<0.01)with no progression to late-stage senescence.At a seeding density of 2500 cells/cm^(2),GMP-compliant DFSC sheets achieved better osteogenic differentiation(P<0.01)and maximal osteogenic,chondrogenic and periostin gene expression(P<0.001),coupled with the highest hydroxyproline secretion(P<0.001)and moderate sulfated glycosaminoglycan production.No statistically significant difference in senescent cell count was observed compared to DFSC sheets at a seeding density of 5000 cells/cm^(2).Supplementation with 25μg/mL L-ascorbic acid significantly enhanced osteogenic gene expression(P<0.001)and elevated hydroxyproline(P<0.01)and sulfated glycosaminoglycan secretion to high ranges.Compared with the cell suspension,the cell sheet demonstrated improved osteogenic,paracrine,and periodontal regenerative capacities in Sprague-Dawley rats.The optimized DFSC sheets demonstrated significantly higher levels of vascular endothelial growth factor and angiopoietin-1(P<0.001)compared to DFSC suspensions,along with enhanced osteogenic induction outcomes(optical density=0.1333±0.01270 vs 0.1007±0.0005774 in suspensions,P<0.05).Following implantation into the rat periodontal defect model,micro-computed tomography analysis revealed superior bone regeneration metrics in the cell sheet group compared to both the cell suspension group and control group(percent bone volume,trabecular thickness,trabecular number),while trabecular spacing exhibited an inverse pattern.CONCLUSION Optimized DFSC sheets cultured under the identified conditions outperform DFSC suspensions.This study contributes to the industrial-scale production of DFSC sheets and establishes a foundation for cell therapy applications.
文摘Periodontitis has emerged as one of the most critical oral diseases, and research on this condition holds great importance for the advancement of stomatology. As the most authoritative national scientific research funding institution in China, the National Natural Science Foundation of China (NSFC) has played a pivotal role in driving the progress of periodontal science by supporting research on periodontitis. This article provides a comprehensive review of the research and development progress related to periodontitis in China from 2014 to 2023, highlighting the significant contributions of the NSFC to this field. We have summarized the detailed funding information from the NSFC, including the number of applicant codes, funded programs and the distribution of funded scholars. These data illustrate the efforts of the NSFC in cultivating young scientists and building research groups to address key challenges in national scientific research. This study offers an overview of the current hot topics, recent breakthroughs and future research prospects related to periodontitis in China.
基金supported by the Scientific and Technological Development Program of Jilin Province,China (Nos.20210203090SF and 20200403119SF)
文摘Inflammation represents a critical immune response triggered by cellular activities and inflammatory mediators following tissue damage.It plays a central role in the pathological progression of diverse diseases,including psychiatric disorders,cancer,and immunological conditions,rendering it an essential target for therapeutic intervention.Periodontitis,a prevalent oral inflammatory disease,is a leading cause of tooth loss and poses significant health challenges globally.Traditionally,inflammatory diseases such as periodontitis have been treated with systemic administration of synthetic chemicals.However,recent years have witnessed challenges,including drug resistance and microbial dysbiosis associated with these treatments.In contrast,natural products derived from Chinese medicine offer numerous benefits,such as high safety profiles,minimal side effects,innovative pharmacological mechanisms,ease of extraction,and multiple targets,rendering them viable alternatives to conventional antibiotics for treating inflammatory conditions.Numerous effective anti-inflammatory natural products have been identified in traditional Chinese medicine(TCM),including alkaloids,flavonoids,terpenoids,lignans,and other natural products that exhibit inhibitory effects on inflammation and are potential therapeutic agents.Several studies have confirmed the substantial anti-inflammatory and immunomodulatory properties of these compounds.This comprehensive review examines the literature on the anti-inflammatory effects of TCM-derived natural products from databases such as PubMed,Web of Science,and CNKI,focusing on terms like"inflammation","periodontitis","pharmacology",and"traditional Chinese medicine".The analysis systematically summarizes the molecular pharmacology,chemical composition,and biological activities of these compounds in inflammatory responses,alongside their mechanisms of action.This research seeks to deepen understanding of the mechanisms and biological activities of herbal extracts in managing inflammatory diseases,potentially leading to the development of promising new anti-inflammatory drug candidates.Future applications could extend to the treatment of various inflammatory conditions,including periodontitis.
文摘The prevalence of cardiometabolic syndrome(CMS)and increasing mortality rate play a significant role in the global increase of cardiovascular disease(CVD)in developing countries.A group of metabolic syndromes that are risk factors for CVDs are referred to as the CMS.Although the exact mechanism(s)behind the development of the CMS are not known,but multi-organ insulin resistance,a prevalent characteristic of the syndrome,is probably one of them.The two most prevalent dental diseases i.e.periodontitis(PD)and dental caries have been re-lated to several systemic diseases and disorders,such as CMS.Age,alcohol con-sumption,being obese,possessing diabetes,as well as smoking are risk factors for periodontal diseases,while both CVD and periodontal diseases are linked to systemic inflammation.It has a multifactorial aetiology and is associated with many systemic diseases.When bacteria and their products attack the periodontal tissues,the tissue raises an immune-inflammatory response against the patho-gens.This acute phase response is a result of the pathogen’s systemic attack and contributes to the overall inflammatory burden of the system.CVD and PD are both diseases associated with systemic inflammation and may be related as they share many common risk factors.Hence,the correlation between these conditions might also have an impact on how dentistry and medicine are practised,thus helping to build a working relationship between the dentist and the physician.
基金supported by the Wenzhou Major Scientific and Technological Innovation Project(No.ZY2022020)the Zhejiang Provincial Science and Technology Project for Public Welfare(No.LGF22H140010)the Science and Technology Plan of Wenzhou(No.H20210008)。
文摘In the fight against bacterial infections,it is critical to effectively disrupt biofilms.However,disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents.Herein,we present a self-propelled nanovesicle(PCL-PLG@CHX)strategy for eliminating biofilms and further expediting the healing of wounds.PCL-PLG@CHX is synthesized by assembling vesicles from amphiphilic polymers,which incorporate both poly-ε-caprolactone and guanidinated-poly-ε-lysine(PCL-PLG)and are infused with chlorhexidine(CHX).Upon application to sites of bacterial infection,PCL-PLG@CHX,abundant in guanidinium structures,effectively accumulates on the negatively charged surface of biofilms.It interacts with reactive oxygen species(ROS)within the biofilm,leading to nitric oxide(NO)production.The generated NO cannot only propel the nanovesicle to penetrate deeper into the biofilm,but also act as a signaling molecule to disperse the biofilm,working in conjunction with the subsequent release of CHX for an enhanced antibacterial impact.Following the eradication of bacteria,the residual guanidine component continues to produce small quantities of NO,facilitating angiogenesis and epithelial growth,thereby accelerating the healing of wounds.Together,our study shows that PCL-PLG@CHX utilizes the potential of guanidine moieties to efficiently break down biofilms and support tissue restoration,tackling the pivotal challenge of biofilm-related diseases.
文摘BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves mechanical debridement and plaque control,but localized antimicrobial therapy offers site-specific advantages.While antibiotics such as metronidazole and doxycycline are commonly used,green tea extract,which is rich in epigallocatechin gallate,has been proposed as a promising alternative for local drug delivery due to its anti-inflammatory and antimicrobial properties.AIM To compare the clinical efficacy of green tea extract gel and ornidazole gel as adjuncts to scaling and root planing(SRP)in patients with periodontitis.METHODS Teeth with probing pocket depths(PPD)of 4-7 mm were selected.Participants’baseline oral hygiene index-simplified,plaque index,clinical attachment loss,and PPD were recorded.The participants were randomized into two groups:One received green tea extract gel after SRP,and the other received ornidazole gel.Subgingival drug delivery was performed,and participants refrained from brushing or interproximal cleaning for ten days.Their clinical parameters were re-evaluated after one month.RESULTS The PPD decreased significantly from baseline to one month in both groups.However,the green tea extract gel group exhibited superior outcomes to the ornidazole gel group,with a mean difference in PPD of 0.28±0.78 mm at one month(P<0.007).CONCLUSION As an adjunct to SRP,green tea extract gel showed greater efficacy in improving clinical periodontal parameters than ornidazole gel.
基金Scientific Research Fund of Education Department of Yunnan Province Project:Potential Targets and Molecular Mechanisms of Sanqi(Radix Notoginseng),an Active Component of Yunnan Baiyao,in the Treatment of Periodontitis(No.2022Y204)Special Project for The Selection of High-level Scientific and Technological Talents and Innovation Teams-technical Innovation Talents Training Object Project:Technical Innovation Personnel Training Object Project(202405AD350005)。
文摘OBJECTIVE:To investigate the effect and mechanism of Sanqi(Radix Notoginseng)in treating periodontitis.METHODS:The active components and periodontitis targets were analyzed through network pharmacology and molecular docking.A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi(Radix Notoginseng)at different doses for 30 d.The alveolar bone structure was observed by micro-CT,the periodontal tissue structure was observed by hematoxylin-eosin staining,and the related proteins changes was detected by immunohistochemical staining.RESULTS:Sanqi(Radix Notoginseng)and periodontitis had a total of 96 coincident targets that were significantly enriched in the interleukin 17(IL-17),tumor necrosis factor(TNF),and advanced glycation endproducts and the receptor of advanced glycation endproducts signaling pathways.The active compound quercetin had good binding activity with interleukin 6(IL-6),vascular endothelial growth factor A(VEGFA),matrix metallopeptidase 9(MMP9),tumor necrosis factorα(TNF-α),Jun proto-oncogene(JUN),and C-X-C motif chemokine ligand 8(CXCL8)in periodontitis.Compared with normal group,the distance from the cementoenamel junction(CEJ)to the alveolar bone(AB)was increased,alveolar bone absorption was obvious,the periodontal tissue structure was disorganized,and IL-6 and TNF-αwere upregulated in periodontitis group;meanwhile,the distance from CEJ to AB was significantly decreased,alveolar bone resorption was reduced,periodontal tissue structure was improved,the expression of IL-6,TNF-α,IL-17 and retinoid-ralated orphan receptorγt(RORγt)were decreased,Forkhead Box P3(FOXP3)and IL-10 were increased after Sanqi(Radix Notoginseng)treatment.CONCLUSIONS:Sanqi(Radix Notoginseng)improves the structure of alveolar bone and gum,and reduces inflammation;the mechanism involve in inhibiting IL-17 signaling pathway to suppress Th17 and promote Treg cells differentiation.
文摘This article explores the association between salivary uric acid(UA)and periodontitis,systematically analyzing its dual roles and research progress.Studies indicate that UA acts as a primary antioxidant in saliva under physiological conditions(accounting for 70%),protecting periodontal tissues by scavenging reactive oxygen species.However,when gum disease becomes severe,UA can switch roles and fuel inflammation,worsening tissue damage.Lorente et al’s research found an independent inverse correlation between salivary UA levels and periodontitis severity(odds ratio=6.14,P=0.001),establishing 111 nmol/mL as a diagnostic threshold(area under the curve=66%).Nevertheless,limitations include sample heterogeneity and failure to distinguish between gingivitis and periodontitis.Mechanistically,three hypotheses are proposed:The Antioxidant Depletion Hypothesis(UA oxidation consumption leading to feedback loops),the Microbial Metabolic Hijacking Hypothesis(pathogens utilizing UA as a carbon source to disrupt redox balance),and the Epithelial Barrier Dysfunction Hypothesis(UA deficiency causing downregulation of tight junction proteins).Future research should prioritize longitudinal cohorts to validate predictive value,integrate multi-omics to explore dysregulated signatures,and develop UA supplementation or targeted antioxidant therapies.This study provides novel insights into periodontitis diagnosis and mechanisms,advancing the application of salivary biomarkers in precision periodontics.
文摘In this letter,we delve into the groundbreaking research by Lorente et al,which sheds light on the intricate relationship between low salivary uric acid levels and periodontitis.The study not only confirms previous observations of reduced salivary uric acid concentrations in periodontitis patients but also establishes,for the first time,an independent association between these two factors,even when controlling for traditional risk factors such as age,smoking status,and arterial hypertension.Moreover,the findings reveal a significant negative correlation between salivary uric acid levels and the severity of periodontitis,suggesting that this biomarker may serve as a valuable indicator of disease progression.These discoveries open new avenues for understanding the pathophysiology of period-ontitis and pave the way for innovative diagnostic and therapeutic strategies.The potential clinical applications of salivary uric acid measurement,such as guiding personalized treatment plans and monitoring disease activity,warrant further exploration to enhance patient care and improve outcomes in this prevalent inflammatory condition.
基金supported by the National Natural Science Foundation of China(Nos.82171768,81701600,and 82001048)the National Key Research and Development Program of China(No.2022YFC3602000)the Young Elite Scientist Support Program by CSA(Chinese Stomatological Association)(No.2020PYRC001).
文摘Rheumatoid arthritis(RA),an autoimmune disease characterized by chronic inflammation of synovial tissue,is divided into two subtypes-anti-citrullinated protein antibody(ACPA)-positive and ACPA-negative RA.While the pathogenic mechanisms of ACPA-positive RA are well-understood,the etiology of ACPA-negative RA remains largely unknown.The association between RA and periodontitis(PD)has been observed since the early 190os,with the two diseases sharing common genetic and environmental risk factors that lead to the progressive destruction of bone and connective tissue.However,the associations between PD and the two subtypes of RA differ.This comprehensive review aims to provide an updated understanding of the epidemiological association between RA and PD,explore potential pathogenic mechanisms linking the two diseases,and highlight the key distinctions between the subtypes of RA and their respective associations with PD.We also discuss the possibility of early intervention or the treatment of the two diseases.Ultimately,this review aims to provide valuable insights for future research in this field.
基金supported by the Tianjin Stomatological Hospital MD and PhD Key Program(No.2019BSZD11)Periodontal Key Discipline Project of Tianjin Stomatological Hospital(2022P02)+1 种基金the Science and Technology Project of Tianjin Health Commission(No.ZC20039)the High-level Talents in the Medical/Health Care Industry-Young Medical Elites(No.TJSQNYXXR-D2-114).
文摘Objective To investigate the treatment effect of the histone demethylase inhibitor GSK-J4,a small molecule that inhibits the demethylase activity of Jumonji domain-containing protein 3(JMJD3),in the treatment of periodontitis.Methods Gingival tissues from patients with moderate to severe chronic periodontitis and healthy controls were collected to evaluate JMJD3 expression via real-time quantitative reverse transcription PCR(RT-qPCR)and immunohistochemistry(IHC).Next,Sprague–Dawley(SD)rats were used to investigate the effect of GSK-J4 in vivo.The experimental periodontitis model was induced by upper first molar ligation and gingival sulcus injection of Porphyromonas gingivalis.The rats were divided into a healthy group,a periodontitis group,periodontitis plus GSK-J4 treatment groups(P+GSK-J415 mg/kg or 25 mg/kg),and a periodontitis plus dimethyl sulfoxide(DMSO)group(P+DMSO).After 4 weeks,maxillary molar segments were assessed via micro-computed tomography(CT)and hematoxylin and eosin(HE)staining.Serum tumor necrosis factor-α(TNF-α)levels were measured by enzyme-linked immunosorbent assay(ELISA).Results Higher expression of the Jmjd3 gene and JMJD3 protein was detected in human inflamed gingiva than in healthy gingiva(P<0.05).GSK-J4 administration reversed alveolar bone absorption[i.e.,reduced alveolar bone crest(ABC)-cementoenamel junction(CEJ)distance],reduced inflammatory cell accumulation at the crest of the alveolar bone,and alleviated serum TNF-αlevels in rats with periodontitis.Moreover,the number of H3K27me3-positive nuclei was greater in model rats treated with GSK J4 than in model rats.Conclusions The histone demethylase inhibitor GSK-J4 attenuated periodontal bone loss and inflammation in a rat periodontitis model by targeting JMJD3.
基金supported in part by the Natural Science and Technology Foundation of Guizhou Province(QiankeheJichu-ZK[2022]Yiban606)National Natural Science Foundation of China(82160181)+1 种基金Science and Technology Foundation of Guizhou Health and Health Committee(gzwkj2024-455)Zunyi Oral Disease Immune Prevention and Medical Biomaterials Research and Development Innovation Talent Team(Zunyi Science Talent[2022]No.1).
文摘Periodontitis is an inflammatory infectious disease affecting the periodontal supporting tissues and is the primary cause of tooth loosening and tooth loss in adults.Clinically,supragingival scaling,subgingival scaling,root planing,and other basic periodontal treatments,often combined with antibiotic therapy,are commonly employed with moderate efficacy.However,the increasing prevalence of antibiotic resistance and associated adverse reactions has become a growing concern.Recent studies have demonstrated the significant impact of traditional Chinese medicine(TCM)extracts in both the prevention and treatment of periodontitis,exhibiting remarkable effectiveness.This review explores the role and mechanisms of TCM extracts in the prevention and treatment of periodontitis,providing a reference for further elucidation of their mechanisms and a theoretical basis for the development of Chinese herbal medicine-based care products.
文摘BACKGROUND Periodontitis is a chronic inflammatory disorder influenced by both behavioral and genetic factors.Among epigenetic regulators,the ANRIL gene has been proposed as a risk factor for periodontitis;however,findings on the association between ANRIL polymorphisms and disease susceptibility remain inconsistent.AIM To analyze the association between the rs1333048 genetic polymorphism in the ANRIL gene and periodontitis via meta-analysis.METHODS A literature search was performed for studies published before May 2,2025.The Review Manager statistical program was used in analyses with calculations of heterogeneity index(I^(2))and odds ratio(OR)with 95%of confidence intervals(CI).Begg’s test and the Egger’s linear regression test were used for publication bias evaluation using Comprehensive meta-analysis software.P<0.05 was considered significant.da Silva FRP et al.ANRIL gene and periodontitis:A meta-analysis WJMA https://www.wjgnet.com 2 December 18,2025 Volume 13 Issue 4 RESULTS From 12 studies including 5489 participants across multiple ethnic groups,we observed a statistically significant association between ANRIL gene polymorphisms and periodontitis in the allelic contrast model(OR=1.2495%CI:1.15-1.34,P<0.00001).Conversely,the wild-type allele was significantly associated with the control group(OR=0.8095%CI:0.75-0.87,P<0.00001).Heterogeneity was low(I²=28%,Pheterogeneity=0.17),and no significant risk of publication bias was detected(P>0.05).CONCLUSION In conclusion,this meta-analysis demonstrated a significant association between the rs1333048 polymorphism and periodontitis in the overall analysis and in stratified analyses of Caucasian populations,but in for mixed-race populations.
基金financially supported by the National Natural Science Funds(NSFC,Nos.U21A20417 and 31930067)1·3·5 project for disciplines of excellence,West China Hospital,Sichuan University(No.ZYGD18002)。
文摘Periodontitis is a chronic inflammatory disease caused by oral pathogens,and the osteogenic potential of human periodontal ligament stem cells(hPDLSCs)is severely impaired under the inflammatory environment.Current clinical periodontitis treatment strategies such as surgical interventions and antibiotic delivery still suffer from poor antibacterial efficacy,difficulty in ameliorating excessive inflammatory responses and slow periodontal tissue regeneration.Here,we have innovatively developed a non-surgical treatment strategy based on a functional composite hydrogel.A composite hydrogel system(Pt@ZIF-8/ALN-ac/Gel)containing bioactive zeolite imidazolate framework-8(ZIF-8)integrated with platinum nanoparticles(Pt@ZIF-8)and alendronate acrylamide(ALN-ac)was constructed on the basis of gelatin methacryloyl(GelMA)to achieve enhanced antibacterial effect and reactive oxygen species(ROS)scavenging ability while promoting the osteogenic potential of hPDLSCs.We confirmed that Pt@ZIF-8/ALN-ac/Gel was able to continuously release Zn^(2+)and exerted an obvious antibacterial effect against Porphyromonas gingivalis.In vitro experiments proved that Pt@ZIF-8/ALN-ac/Gel had good biocompatibility,while efficiently featuring excellent reactive oxygen species(ROS)scavenging capacity,increasing alkaline phosphatase activity,and promoting extracellular matrix mineralization by hPDLSCs.In vivo,Pt@ZIF-8/ALN-ac/Gel significantly inhibited the alveolar bone deterioration and reduced osteoclast activation and inflammation,thereby promoting the regeneration of damaged tissues.These findings demonstrated superior therapeutic efficacy in the reported clinical periodontitis treatment,exhibiting great potential for application.
文摘Periodontitis is the commonest oral disease affecting population worldwide. This disease is notorious for the devastation of tooth supporting structures, ensuing in the loss of dentition. The etiology for this disease is bacterial biofilm, which accumulates on the teeth as dental plaque. In addition to the biofilm microorganisms, other factors such as environmental, systemic and genetic are also responsible in progression of periodontitis. Diabetes mellitus (DM) is metabolic disorder which has an impact on the global health. DM plays a crucial role in the pathogenesis of periodontitis. Periodontitis is declared as the “sixth” major complication of DM. Evidence based literature has depicted an enhanced incidence and severity of periodontitis in subjects with DM. A “two way” relationship has been purported between periodontitis and DM. Mutual management of both conditions is necessary. Periodontal therapy (PT) may assist to diminish the progression of DM and improve glycemic control. Various advanced technological facilities may be utilized for the purpose of patient education and disease management. The present paper clarifies the etio-pathogenesis of periodontitis, establishing it as a complication of DM and elaborating the various mechanisms involved in the pathogenesis. The role of PT in amelioration of DM and application of digital communication will be discussed. Overall, it is judicious to create an increased patient cognizance of the periodontitis-DM relationship. Conjunctive efforts must be undertaken by the medical and oral health care professionals for the management of periodontitis affected DM patients.
基金supported by Natural Science Research Program of Henan Education Department(No.201302X63)
文摘Objective: To discuss the expression of RUNX2 and MDM21 in rats with periodontitis under the chronic intermittent hypoxia. Methods: A total of 32 SD healthy rats were randomly divided into four groups, with 8 rats in each group. The molecular biological techniques of immunohistochemistry, RT-PCR and Western blotting were employed to detect the effect of different hypoxia time(0, 6, 12, 24 and 48 h) and different concentrations of hypoxia(0.000, 0.001, 0.010, 0.060 and 0.100 ppm) on the expression of RUNX2 and MDM21 in rats of four groups. Results: The expression of RUNX2 and MDM21 in each group was significantly higher than the one at other concentrations when the concentration was 0.010 ppm, with the statistical difference(P<0.05). The expression of RUNX2 and MDM21 was that normoxic control group > periodontitis group> chronic intermittent hypoxia group > compound group under the action with the concentration of 0.010 ppm for 12 h, but there was no significant difference for the comparison among groups(P>0.05). Conclusions: The condition of chronic intermittent hypoxia can reduce the expression of RUNX2 and MDM21 in rats with periodontitis and aggravate the damage of periodontal bone.
