This study investigated the effects of isomaltooligosaccharide(IMO)and Bacillus in perinatal diets on the duration of farrowing and post-weaning estrus,serum reproductive hormone concentrations,and gut microbiota and ...This study investigated the effects of isomaltooligosaccharide(IMO)and Bacillus in perinatal diets on the duration of farrowing and post-weaning estrus,serum reproductive hormone concentrations,and gut microbiota and its metabolites of sows.Multiparous sows(n=130)were fed diets without IMO(control,CON group),or diets containing only IMO(IMO group),IMO and Bacillus subtilis(IMOS group),IMO and Bacillus licheniformis(IMOL group),and IMO and B.subtilis and B.licheniformis(IMOSL group),respectively.The results indicate that the duration of farrowing and post-weaning estrus was shorter in sows in the IMOS,IMOL,and IMOSL groups,and the weaning-estrous interval was lower in sows in the IMOL greoup.In addition,the lowest fecal score was observed in the IMOL group during d 106 to 112 of gestation.Sows in most of the treatment groups had a higher concentration of serum prolactin and prostaglandin at farrowing,but a lower serum concentration of estradiol,oxytocin,and progesterone on d 18 of lactation than sows in the CON group.The treatment groups had a higher abundance of Candidatus Methanoplasma and Bacillus and a lower abundance of Escherichia-Shigella in their feces at farrowing.Furthermore,the treatment groups had higher concentrations of total short-chain fatty acids(SCFA)in feces at farrowing and a higher concentration of branched fatty acids in feces on d 18 of lactation.Furthermore,the abun-dance of Bacillus in feces was positively correlated with serum prostaglandin concentrations and fecal total SCFA of sows at farrowing,but was negatively correlated with the duration of farrowing.Overall,dietary IMO and Bacillus supplementation affected the concentration of serum reproductive hormones and the duration of farrowing and post-weaning estrus,and the gut microbiota is a key factor.展开更多
The transition period for dairy cows usually refers to the 3 weeks pre-calving to the 3 weeks post-calving.During this period,dairy cows undergo metabolic and physiological adaptations because of their susceptibility ...The transition period for dairy cows usually refers to the 3 weeks pre-calving to the 3 weeks post-calving.During this period,dairy cows undergo metabolic and physiological adaptations because of their susceptibility to metabolic and infectious diseases.Poor feeding management under these circumstances may adversely affect the health and subsequent production performance of the cows.Owing to long-term adaptation and evolution,the rumen has become a unique ecosystem inhabited by a complex microbial community closely associated with its natural host.Dietary components are metabolized by the rumen microbiota,and volatile fatty acids and microbial protein products can be used as precursor substances for synthesizing meat and milk components.The successful transition of perinatal dairy cows includes changes in diet,physiology,and the rumen microbiota.Rumen microbial profiles have been confirmed to be heritable and repairable;however,adverse circumstances affect rumen microbial composition,host digestion and metabolism,as well as postpartum production traits of dairy cows for a certain period.Preliminary evidence indicates a close relationship between the rumen microbiota and animal performance.Therefore,changes in rumen microbes during the transition period and the intrinsic links between the microbiota and host postpartum phenotypic traits need to be better understood to optimize production performance in ruminants.展开更多
Describe the content and current situation of maternal information needs and support,providing a basis for building maternal information needs assessment tools and improving information support systems.Retrieve articl...Describe the content and current situation of maternal information needs and support,providing a basis for building maternal information needs assessment tools and improving information support systems.Retrieve articles related to the topic from domestic and foreign databases,and ultimately include 54 articles.Summarize from the aspects of information demand content,influencing factors,evaluation tools,and information support channels.We found that the information needs of pregnant women are rich in content,but the existing information support content is limited and the form is single.There is an urgent need to establish scientific and effective information needs assessment tools,as well as diverse information support systems.展开更多
Objective:To compare the effects of electroacupuncture(EA)and moxibustion at Zusanli(ST 36)on the lung phenotype of rat offspring exposed to nicotine during the perinatal period.Methods:Pregnant Sprague-Dawley rats we...Objective:To compare the effects of electroacupuncture(EA)and moxibustion at Zusanli(ST 36)on the lung phenotype of rat offspring exposed to nicotine during the perinatal period.Methods:Pregnant Sprague-Dawley rats were randomly divided into 4 groups:the control group(saline only),the model group(nicotine only),the EA group(nicotine+EA at ST 36 acupoints bilaterally),and the moxibustion group(nicotine+moxibustion at ST 36 acupoints bilaterally).n=6 rats per group.On postnatal day 21,the body weight,lung weight,and pulmonary function were determined and lung morphometry was performed.Peroxisome proliferator-activated receptor gamma andβ-catenin levels in the lung tissue of offspring were also determined.Results:Perinatal nicotine exposure(PNE)results in decreased body and lung weights of offspring rats,abnormal lung tissue morphology,and significantly altered pulmonary function,showing an increase in total airway resistance and a decrease in tidal volume,minute ventilation,total airway compliance,and peak expiratory flow.Bilateral EA at ST 36 acupoints could block all of these perinatal nicotine-induced effects.Although moxibustion also had protective effects in nicotine-induced offspring lungs,some of these effects did not reach statistical significance,e.g.,protection against the upregulation ofβ-catenin,the downregulation of PPARγsignaling,and the increase in peak expiratory flow.Conclusion:Maternal EA at ST 36 blocked the PNE-induced changes in key developmental signaling pathways,prevented the PNE-induced changes in lung morphology,and protected pulmonary function.Moxibustion at ST 36 showed similar but weaker protective effects against the PNE-induced changes in the exposed offspring.It is important to note that the mechanism underlying the protective effects of moxibustion at ST 36 may be different from those of EA at ST 36,and further research is needed to understand these differences.展开更多
The aim of this study was to investigate the effects of </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;...The aim of this study was to investigate the effects of </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation products on performance, blood hormone levels and rumen floral composition in peripartum dairy cows. Sixty perinatal cows were selected and allocated to two groups according to parity and expected date of delivery. Each group was supplemented with </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation product 0 or 100 g. The results showed that </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation products could significantly increase the feed intake of peripartum dairy cows (P < 0.01), increase the lactose content after 21 days postpartum (P < 0.01), and tend to increase milk production (P = 0.052). There was no significant effect on other milk components, the apparent digestibility of nutrients. There was a tendency to increase milk production and reduce the number of somatic cells in milk;increase blood levels of glucagon (P < 0.01) and </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-hydroxybutyrate (P = 0.01), reducing the insulin content (P = 0.02).</span></span><span style="font-family:""> </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:""><span style="font-family:Verdana;"> reduced the abundance of rumen microbes in peripartum dairy cows but had no effect on rumen microbial diversity. Compared with the control group, the supplemented group showed reductions in the abundance of genera </span><i><span style="font-family:Verdana;">Bacillus</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Butyrivibrio</span></i><span style="font-family:Verdana;"> (P = 0.01), </span><i><span style="font-family:Verdana;">Denitrobacterium</span></i><span style="font-family:Verdana;"> (P = 0.01), and </span><i><span style="font-family:Verdana;">Mogibacterium</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Porphyromonas</span></i><span style="font-family:Verdana;"> (P = 0.05), </span><i><span style="font-family:Verdana;">Saccharofermentans</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Sphaerochaeta</span></i><span style="font-family:Verdana;"> (P = 0.02), </span><i><span style="font-family:Verdana;">Streptococcus</span></i><span style="font-family:Verdana;"> (P = 0.04) and other genera. There were significant increase in the content of </span><i><span style="font-family:Verdana;">Acidaminococcus</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Allisonella</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Bulleidia</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Corynebacterium</span></i><span style="font-family:Verdana;"> (P = 0.01), </span><i><span style="font-family:Verdana;">Dialister</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Faecalibacterium</span></i><span style="font-family:Verdana;"> (P = 0.02), </span><i><span style="font-family:Verdana;">Faekalitalea</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Fibrobacter</span></i><span style="font-family:Verdana;"> (P = 0.04), </span><i><span style="font-family:Verdana;">Flavobacterium</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Kandleria</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Paraprevotella</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Pyramidobacter</span></i><span style="font-family:Verdana;"> (P = 0.05), </span><i><span style="font-family:Verdana;">Roseburia</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Succinivibrio</span></i><span style="font-family:Verdana;"> (P < 0.01) and other genera.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The main metabolic pathways such as tryptophan metabolism and steroid hormone biosynthesis in perinatal dairy cows were determined for </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation products.展开更多
Objective To observe the toxic effect of the mixture of organophosphorus pesticide (MOP) on maternal rats and on the growth and development of their offspring. Methods Totally 40 Sprague-Dawley pregnant rats were rand...Objective To observe the toxic effect of the mixture of organophosphorus pesticide (MOP) on maternal rats and on the growth and development of their offspring. Methods Totally 40 Sprague-Dawley pregnant rats were randomly divided into three MOP dose groups and one control to which their offspring would be assigned. The experimental dosage of MOP and distilled water were administered orally starting on gestation day 15 and continued for 35 days. The physical development indices and the learning ability of F1 rats were measured during lactation. The pathological changes of uterus and liver of F0 rats were observed after weaning, while the weight ratio of uterus and some viscera to body of the F1 were examined. Results There were obvious changes of uterus and liver in the high-dose group of F0. The body-weight accretion of the F1 in high-dose group was obviously lower than that in control group (P<0.05). Some of the MOP F1 rats development indices delayed significantly (P<0.05), the learning ability decreased obviously, and the time of setting up memory prolonged (P<0.05). The ratio of the uterus weight to body-weight in the F1 MOP groups was significantly higher than that in control group (P<0.05). Conclusion The experiment doses of MOP are proved to have significant reproductive toxicity on perinatal rats.展开更多
Hydroxyproline is the main component of collagen,accounting for 20% or so of collagen,and metabolic changes in bone collagen directly affect the level of hydroxyproline in the blood.So inspecting the content of hydrox...Hydroxyproline is the main component of collagen,accounting for 20% or so of collagen,and metabolic changes in bone collagen directly affect the level of hydroxyproline in the blood.So inspecting the content of hydroxyproline is very important for pathogenesis and prophylactico-therapeutic measures on osteodystrophy of dairy cows.This experiment monitored and studied the change regularity of serum calcium,serum phosphorus and serum free hydroxyproline of perinatal cow in Hebei Province,and discussed a new and early diagnosis way for osteodystrophy.Thus,effective control measures could be implemented before the bone became deformed without any clinical symptom.The paper provided a theoretical foundation for early diagnosis of perinatal cow.展开更多
Objective: to study and analyze the effects of perinatal comprehensive management on glucose and lipid metabolism and maternal and infant outcomes in patients with gestational diabetes. Methods: 60 patients with gesta...Objective: to study and analyze the effects of perinatal comprehensive management on glucose and lipid metabolism and maternal and infant outcomes in patients with gestational diabetes. Methods: 60 patients with gestational diabetes admitted to our hospital from February 2020 to may 2022 were included. They were divided into the observation group and the control group (n 30) by double-blind random method. The control group carried out routine treatment of gestational diabetes, and the observation group carried out comprehensive perinatal management on the basis of routine treatment. Statistics and comparison were made on insulin related indicators, glucose and lipid metabolism levels, and maternal and infant outcomes between the two groups. Results: around 24 hours after delivery, the insulin resistance index of the observation group was lower than that of the control group. After delivery, the fasting blood glucose, glycosylated hemoglobin, total cholesterol and other glycolipid metabolism indexes of the observation group were lower than that of the control group. The incidence of adverse maternal and infant outcomes was lower than that of the control group, and the difference was statistically significant (P 0.05). Conclusion: the application of perinatal comprehensive management in patients with gestational diabetes is helpful to improve insulin resistance index, glucose and lipid metabolism and maternal and infant outcomes.展开更多
Objective: the whole process of pregnant women from pregnancy to delivery is a special period. Because of the influence of pregnancy, many changes will take place in pregnant women's bodies. Although the changes a...Objective: the whole process of pregnant women from pregnancy to delivery is a special period. Because of the influence of pregnancy, many changes will take place in pregnant women's bodies. Although the changes are mainly physiological changes, these changes will gradually become obvious with the pregnant women's pregnancy, and it is easy for pregnant women to have certain psychological pressure. Many hospitals mainly pay attention to the daily health care and the handling of unexpected events during the clinical nursing of pregnant and lying-in women, but pay little attention to health education. Especially the opening of the second-child policy in China, due to the increase of the number of pregnant women, the obstetrics and gynecology department is facing great work pressure. Among these pregnant women, high-collar women and high-risk women occupy a certain number. If we can't give scientific health education to pregnant women, the pregnant women's self-health cognitive ability is insufficient, which will even affect the delivery outcome. In addition, the emotional and psychological changes of pregnant women have obvious changes during pregnancy. It's easy to get too excited because of some small things. If you can't get a good dredge, you may have anxiety, depression and other performances. The existence of these negative factors will have a certain degree of impact on the health care of pregnant women during pregnancy. Therefore, this paper will explore the perinatal health education. Methods: China is a populous country. With the implementation of relevant policies, the number of new pregnant women in China is increasing every year. In order to improve the comfort and safety of pregnant women's care, this time, some pregnant women were selected from the department as the research object, and 76 pregnant women who met the requirements were selected. They were randomly divided into groups by computer and given clinical care according to their conditions. This time, it was determined as routine perinatal care and perinatal health education intervention. Patients in the control group were given the first type of nursing program, while other patients were given health education. After nursing, a comprehensive investigation was made on the patients' knowledge and cognition, living conditions, and whether they had bad psychological conditions. Results: some parturients are multiparas, who have a certain knowledge base about pregnancy and childbirth, and can keep a good psychological state during pregnancy. But for primiparas, because they are the first "mothers", they don't know a lot of health knowledge, and because they don't understand, they are prone to all kinds of unpredictable events during pregnancy. Therefore, this time, nursing intervention was carried out in this respect. The data showed that after intervention, the pregnant women in the observation group knew more about related knowledge, and their cognitive level was obviously improved. The nursing behavior of the department has a great influence on the parturient. If proper nursing can be given, the self-care ability of the parturient can be improved and the quality of life can be guaranteed. This evaluation shows that the living conditions of some parturient women in the control group are not in line with expectations. Pregnant women will have all kinds of discomfort due to pregnancy, such as morning sickness and grumpy temper. If they can't get the understanding of their families, they are more likely to have psychological problems. In order to promote the pregnant women to self-regulate their emotions and keep the sunshine's psychology and emotions, this intervention is made in this respect. Most pregnant women in the observation group have little emotional fluctuation and good psychological state, and the difference of data results is statistically significant (P<0.05). Conclusion: clinical practice shows that the implementation of health education in the perinatal period of pregnant women can make them have a comprehensive grasp and understanding of health knowledge, improve the level of knowledge cognition of pregnant women, and have a positive impact on the mental and psychological aspects of pregnant women through this measure.展开更多
Objectives:To investigate the effects of various complications on the coagulation function of perinatal women.Methods:A total of 210 pregnant women between November 2016 and June 2017 were recruited.The coagulation fu...Objectives:To investigate the effects of various complications on the coagulation function of perinatal women.Methods:A total of 210 pregnant women between November 2016 and June 2017 were recruited.The coagulation function indexes,including prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),and thrombin time(TT),were evaluated in pregnant women with group B Streptococcus(GBS),positive nonstress test(NST),scarred uterus,prenatal fever,macrosomia,gestational hypertension,gestational diabetes,premature rupture of membranes(PROMs),threatened abortion,and oligohydramnios.Results:No significant differences were found in PT,APTT,FIB,and TT in pregnant women with GBS,positive NST,scarred uterus,macrosomia,gestational hypertension,gestational diabetes,PROMs,and oligohydramnios.Moreover,significantly decreased PT and APTT and significantly increased TT were found in pregnant women with prenatal fever compared to normal controls,whereas significantly increased TT was also observed in those with threatened abortion.Conclusions:Among the complications that occur in pregnant women,prenatal fever and threatened abortion have important effects on coagulation function.展开更多
基金funding from Double first-class construction project of Hunan Agricultural University(SYL201802009,SYL201802015)the National Key Research and Development Program of China(2017YFD0500506,2016YFD0501209).
文摘This study investigated the effects of isomaltooligosaccharide(IMO)and Bacillus in perinatal diets on the duration of farrowing and post-weaning estrus,serum reproductive hormone concentrations,and gut microbiota and its metabolites of sows.Multiparous sows(n=130)were fed diets without IMO(control,CON group),or diets containing only IMO(IMO group),IMO and Bacillus subtilis(IMOS group),IMO and Bacillus licheniformis(IMOL group),and IMO and B.subtilis and B.licheniformis(IMOSL group),respectively.The results indicate that the duration of farrowing and post-weaning estrus was shorter in sows in the IMOS,IMOL,and IMOSL groups,and the weaning-estrous interval was lower in sows in the IMOL greoup.In addition,the lowest fecal score was observed in the IMOL group during d 106 to 112 of gestation.Sows in most of the treatment groups had a higher concentration of serum prolactin and prostaglandin at farrowing,but a lower serum concentration of estradiol,oxytocin,and progesterone on d 18 of lactation than sows in the CON group.The treatment groups had a higher abundance of Candidatus Methanoplasma and Bacillus and a lower abundance of Escherichia-Shigella in their feces at farrowing.Furthermore,the treatment groups had higher concentrations of total short-chain fatty acids(SCFA)in feces at farrowing and a higher concentration of branched fatty acids in feces on d 18 of lactation.Furthermore,the abun-dance of Bacillus in feces was positively correlated with serum prostaglandin concentrations and fecal total SCFA of sows at farrowing,but was negatively correlated with the duration of farrowing.Overall,dietary IMO and Bacillus supplementation affected the concentration of serum reproductive hormones and the duration of farrowing and post-weaning estrus,and the gut microbiota is a key factor.
基金upported by the National Key R&D Program of China (22022YFD1301000)the Agnicultural Science and Technology Innovation Program (ASTIP-IAS12)the earmarked fund for CARS (CARS-36).
文摘The transition period for dairy cows usually refers to the 3 weeks pre-calving to the 3 weeks post-calving.During this period,dairy cows undergo metabolic and physiological adaptations because of their susceptibility to metabolic and infectious diseases.Poor feeding management under these circumstances may adversely affect the health and subsequent production performance of the cows.Owing to long-term adaptation and evolution,the rumen has become a unique ecosystem inhabited by a complex microbial community closely associated with its natural host.Dietary components are metabolized by the rumen microbiota,and volatile fatty acids and microbial protein products can be used as precursor substances for synthesizing meat and milk components.The successful transition of perinatal dairy cows includes changes in diet,physiology,and the rumen microbiota.Rumen microbial profiles have been confirmed to be heritable and repairable;however,adverse circumstances affect rumen microbial composition,host digestion and metabolism,as well as postpartum production traits of dairy cows for a certain period.Preliminary evidence indicates a close relationship between the rumen microbiota and animal performance.Therefore,changes in rumen microbes during the transition period and the intrinsic links between the microbiota and host postpartum phenotypic traits need to be better understood to optimize production performance in ruminants.
文摘Describe the content and current situation of maternal information needs and support,providing a basis for building maternal information needs assessment tools and improving information support systems.Retrieve articles related to the topic from domestic and foreign databases,and ultimately include 54 articles.Summarize from the aspects of information demand content,influencing factors,evaluation tools,and information support channels.We found that the information needs of pregnant women are rich in content,but the existing information support content is limited and the form is single.There is an urgent need to establish scientific and effective information needs assessment tools,as well as diverse information support systems.
基金supported by the National Natural Science Foundation of China(81674059 and 82174505)the Postgraduate Project of Beijing University of Chinese Medicine(2018-JYB22-XS116)+1 种基金the Tobacco-Related Disease Research Program(27IP-0050,and T29IR0737)the National Institutes of Health(HL151769)。
文摘Objective:To compare the effects of electroacupuncture(EA)and moxibustion at Zusanli(ST 36)on the lung phenotype of rat offspring exposed to nicotine during the perinatal period.Methods:Pregnant Sprague-Dawley rats were randomly divided into 4 groups:the control group(saline only),the model group(nicotine only),the EA group(nicotine+EA at ST 36 acupoints bilaterally),and the moxibustion group(nicotine+moxibustion at ST 36 acupoints bilaterally).n=6 rats per group.On postnatal day 21,the body weight,lung weight,and pulmonary function were determined and lung morphometry was performed.Peroxisome proliferator-activated receptor gamma andβ-catenin levels in the lung tissue of offspring were also determined.Results:Perinatal nicotine exposure(PNE)results in decreased body and lung weights of offspring rats,abnormal lung tissue morphology,and significantly altered pulmonary function,showing an increase in total airway resistance and a decrease in tidal volume,minute ventilation,total airway compliance,and peak expiratory flow.Bilateral EA at ST 36 acupoints could block all of these perinatal nicotine-induced effects.Although moxibustion also had protective effects in nicotine-induced offspring lungs,some of these effects did not reach statistical significance,e.g.,protection against the upregulation ofβ-catenin,the downregulation of PPARγsignaling,and the increase in peak expiratory flow.Conclusion:Maternal EA at ST 36 blocked the PNE-induced changes in key developmental signaling pathways,prevented the PNE-induced changes in lung morphology,and protected pulmonary function.Moxibustion at ST 36 showed similar but weaker protective effects against the PNE-induced changes in the exposed offspring.It is important to note that the mechanism underlying the protective effects of moxibustion at ST 36 may be different from those of EA at ST 36,and further research is needed to understand these differences.
文摘The aim of this study was to investigate the effects of </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation products on performance, blood hormone levels and rumen floral composition in peripartum dairy cows. Sixty perinatal cows were selected and allocated to two groups according to parity and expected date of delivery. Each group was supplemented with </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation product 0 or 100 g. The results showed that </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation products could significantly increase the feed intake of peripartum dairy cows (P < 0.01), increase the lactose content after 21 days postpartum (P < 0.01), and tend to increase milk production (P = 0.052). There was no significant effect on other milk components, the apparent digestibility of nutrients. There was a tendency to increase milk production and reduce the number of somatic cells in milk;increase blood levels of glucagon (P < 0.01) and </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-hydroxybutyrate (P = 0.01), reducing the insulin content (P = 0.02).</span></span><span style="font-family:""> </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:""><span style="font-family:Verdana;"> reduced the abundance of rumen microbes in peripartum dairy cows but had no effect on rumen microbial diversity. Compared with the control group, the supplemented group showed reductions in the abundance of genera </span><i><span style="font-family:Verdana;">Bacillus</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Butyrivibrio</span></i><span style="font-family:Verdana;"> (P = 0.01), </span><i><span style="font-family:Verdana;">Denitrobacterium</span></i><span style="font-family:Verdana;"> (P = 0.01), and </span><i><span style="font-family:Verdana;">Mogibacterium</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Porphyromonas</span></i><span style="font-family:Verdana;"> (P = 0.05), </span><i><span style="font-family:Verdana;">Saccharofermentans</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Sphaerochaeta</span></i><span style="font-family:Verdana;"> (P = 0.02), </span><i><span style="font-family:Verdana;">Streptococcus</span></i><span style="font-family:Verdana;"> (P = 0.04) and other genera. There were significant increase in the content of </span><i><span style="font-family:Verdana;">Acidaminococcus</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Allisonella</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Bulleidia</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Corynebacterium</span></i><span style="font-family:Verdana;"> (P = 0.01), </span><i><span style="font-family:Verdana;">Dialister</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Faecalibacterium</span></i><span style="font-family:Verdana;"> (P = 0.02), </span><i><span style="font-family:Verdana;">Faekalitalea</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Fibrobacter</span></i><span style="font-family:Verdana;"> (P = 0.04), </span><i><span style="font-family:Verdana;">Flavobacterium</span></i><span style="font-family:Verdana;"> (P = 0.03), </span><i><span style="font-family:Verdana;">Kandleria</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Paraprevotella</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Pyramidobacter</span></i><span style="font-family:Verdana;"> (P = 0.05), </span><i><span style="font-family:Verdana;">Roseburia</span></i><span style="font-family:Verdana;"> (P < 0.01), </span><i><span style="font-family:Verdana;">Succinivibrio</span></i><span style="font-family:Verdana;"> (P < 0.01) and other genera.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The main metabolic pathways such as tryptophan metabolism and steroid hormone biosynthesis in perinatal dairy cows were determined for </span><i><span style="font-family:Verdana;">Saccharomyces cerevisiae</span></i><span style="font-family:Verdana;"> and its fermentation products.
基金This work was supported by the Natural Science Foundation of Xi an Jiaotong University (No573012)
文摘Objective To observe the toxic effect of the mixture of organophosphorus pesticide (MOP) on maternal rats and on the growth and development of their offspring. Methods Totally 40 Sprague-Dawley pregnant rats were randomly divided into three MOP dose groups and one control to which their offspring would be assigned. The experimental dosage of MOP and distilled water were administered orally starting on gestation day 15 and continued for 35 days. The physical development indices and the learning ability of F1 rats were measured during lactation. The pathological changes of uterus and liver of F0 rats were observed after weaning, while the weight ratio of uterus and some viscera to body of the F1 were examined. Results There were obvious changes of uterus and liver in the high-dose group of F0. The body-weight accretion of the F1 in high-dose group was obviously lower than that in control group (P<0.05). Some of the MOP F1 rats development indices delayed significantly (P<0.05), the learning ability decreased obviously, and the time of setting up memory prolonged (P<0.05). The ratio of the uterus weight to body-weight in the F1 MOP groups was significantly higher than that in control group (P<0.05). Conclusion The experiment doses of MOP are proved to have significant reproductive toxicity on perinatal rats.
基金Supported by Natural Science Foundation of Hebei Province(2009)Modern Agricultural Research Program of Hebei Department of Science and Technology(2013)
文摘Hydroxyproline is the main component of collagen,accounting for 20% or so of collagen,and metabolic changes in bone collagen directly affect the level of hydroxyproline in the blood.So inspecting the content of hydroxyproline is very important for pathogenesis and prophylactico-therapeutic measures on osteodystrophy of dairy cows.This experiment monitored and studied the change regularity of serum calcium,serum phosphorus and serum free hydroxyproline of perinatal cow in Hebei Province,and discussed a new and early diagnosis way for osteodystrophy.Thus,effective control measures could be implemented before the bone became deformed without any clinical symptom.The paper provided a theoretical foundation for early diagnosis of perinatal cow.
文摘Objective: to study and analyze the effects of perinatal comprehensive management on glucose and lipid metabolism and maternal and infant outcomes in patients with gestational diabetes. Methods: 60 patients with gestational diabetes admitted to our hospital from February 2020 to may 2022 were included. They were divided into the observation group and the control group (n 30) by double-blind random method. The control group carried out routine treatment of gestational diabetes, and the observation group carried out comprehensive perinatal management on the basis of routine treatment. Statistics and comparison were made on insulin related indicators, glucose and lipid metabolism levels, and maternal and infant outcomes between the two groups. Results: around 24 hours after delivery, the insulin resistance index of the observation group was lower than that of the control group. After delivery, the fasting blood glucose, glycosylated hemoglobin, total cholesterol and other glycolipid metabolism indexes of the observation group were lower than that of the control group. The incidence of adverse maternal and infant outcomes was lower than that of the control group, and the difference was statistically significant (P 0.05). Conclusion: the application of perinatal comprehensive management in patients with gestational diabetes is helpful to improve insulin resistance index, glucose and lipid metabolism and maternal and infant outcomes.
文摘Objective: the whole process of pregnant women from pregnancy to delivery is a special period. Because of the influence of pregnancy, many changes will take place in pregnant women's bodies. Although the changes are mainly physiological changes, these changes will gradually become obvious with the pregnant women's pregnancy, and it is easy for pregnant women to have certain psychological pressure. Many hospitals mainly pay attention to the daily health care and the handling of unexpected events during the clinical nursing of pregnant and lying-in women, but pay little attention to health education. Especially the opening of the second-child policy in China, due to the increase of the number of pregnant women, the obstetrics and gynecology department is facing great work pressure. Among these pregnant women, high-collar women and high-risk women occupy a certain number. If we can't give scientific health education to pregnant women, the pregnant women's self-health cognitive ability is insufficient, which will even affect the delivery outcome. In addition, the emotional and psychological changes of pregnant women have obvious changes during pregnancy. It's easy to get too excited because of some small things. If you can't get a good dredge, you may have anxiety, depression and other performances. The existence of these negative factors will have a certain degree of impact on the health care of pregnant women during pregnancy. Therefore, this paper will explore the perinatal health education. Methods: China is a populous country. With the implementation of relevant policies, the number of new pregnant women in China is increasing every year. In order to improve the comfort and safety of pregnant women's care, this time, some pregnant women were selected from the department as the research object, and 76 pregnant women who met the requirements were selected. They were randomly divided into groups by computer and given clinical care according to their conditions. This time, it was determined as routine perinatal care and perinatal health education intervention. Patients in the control group were given the first type of nursing program, while other patients were given health education. After nursing, a comprehensive investigation was made on the patients' knowledge and cognition, living conditions, and whether they had bad psychological conditions. Results: some parturients are multiparas, who have a certain knowledge base about pregnancy and childbirth, and can keep a good psychological state during pregnancy. But for primiparas, because they are the first "mothers", they don't know a lot of health knowledge, and because they don't understand, they are prone to all kinds of unpredictable events during pregnancy. Therefore, this time, nursing intervention was carried out in this respect. The data showed that after intervention, the pregnant women in the observation group knew more about related knowledge, and their cognitive level was obviously improved. The nursing behavior of the department has a great influence on the parturient. If proper nursing can be given, the self-care ability of the parturient can be improved and the quality of life can be guaranteed. This evaluation shows that the living conditions of some parturient women in the control group are not in line with expectations. Pregnant women will have all kinds of discomfort due to pregnancy, such as morning sickness and grumpy temper. If they can't get the understanding of their families, they are more likely to have psychological problems. In order to promote the pregnant women to self-regulate their emotions and keep the sunshine's psychology and emotions, this intervention is made in this respect. Most pregnant women in the observation group have little emotional fluctuation and good psychological state, and the difference of data results is statistically significant (P<0.05). Conclusion: clinical practice shows that the implementation of health education in the perinatal period of pregnant women can make them have a comprehensive grasp and understanding of health knowledge, improve the level of knowledge cognition of pregnant women, and have a positive impact on the mental and psychological aspects of pregnant women through this measure.
基金This work was financially supported by the Chinese National Nature Sciences Foundation(grant nos.81471416 and 81771524).
文摘Objectives:To investigate the effects of various complications on the coagulation function of perinatal women.Methods:A total of 210 pregnant women between November 2016 and June 2017 were recruited.The coagulation function indexes,including prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),and thrombin time(TT),were evaluated in pregnant women with group B Streptococcus(GBS),positive nonstress test(NST),scarred uterus,prenatal fever,macrosomia,gestational hypertension,gestational diabetes,premature rupture of membranes(PROMs),threatened abortion,and oligohydramnios.Results:No significant differences were found in PT,APTT,FIB,and TT in pregnant women with GBS,positive NST,scarred uterus,macrosomia,gestational hypertension,gestational diabetes,PROMs,and oligohydramnios.Moreover,significantly decreased PT and APTT and significantly increased TT were found in pregnant women with prenatal fever compared to normal controls,whereas significantly increased TT was also observed in those with threatened abortion.Conclusions:Among the complications that occur in pregnant women,prenatal fever and threatened abortion have important effects on coagulation function.
