The migratory locust Locusta migratoria(Orthoptera,Acrididae)is one of the most important pests due to its extensive and severe damage to crops in large parts of Africa and Asia.Biodegradable and ecologically natural ...The migratory locust Locusta migratoria(Orthoptera,Acrididae)is one of the most important pests due to its extensive and severe damage to crops in large parts of Africa and Asia.Biodegradable and ecologically natural products such as bota nical insecticides are emergi ng can didates for replaceme nt of usually applied chemical pesticides.The crude methanolic extract of Pergularia tomentosa(PME)was investigated for their toxicity and physiological aspects on L.migratoria nymphs.Results showed that treatment of newly emerged fourth and fifth instar nymphs resulted in significant mortality and significant repellent activity with an LC50 value of 0.18 and 0.38%,respectively,after seven days of treatment.The PME toxicity was also dem on strated by histopathological cha nges in the alime ntary canal resulting in con siderable disorganization and severe damage of the caeca and proventriculus structure.The extract induced cellular immune reactions which manifested by a significant decrease in the number of the differential haemocyte counts(prohemocytes and plasmatocytes)and important cell lysis.Data of biochemical analyses showed that the PME reduced the activity of acetylcholinesterase and induced the glutathione S-transferases.The neurotoxic effect was confirmed by the histological alterations in the brain structure,particularly in the neurosecretory cells showing typical signs of cell necrosis.展开更多
Objective:To evaluate therapeutic potential of hydroethanolic extract of Pergularia daemia(P.daemia) against anti-tuberculosis drugs(ATDs) induced liver injury.Methods:Wistar albino rats were divided into seven groups...Objective:To evaluate therapeutic potential of hydroethanolic extract of Pergularia daemia(P.daemia) against anti-tuberculosis drugs(ATDs) induced liver injury.Methods:Wistar albino rats were divided into seven groups of six animal in each.The ATDs and P.daemia extract(100,200 and 400 mg/kg,p.o.) were conjointly administered for 8 weeks and various biochemical,histoarchitectural,ultrastructural studies were performed.Results:Administration of ATDs significantly increased aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,triglycerides,cholesterol,bilirubin and decreased glucose and albumin level.Increased lipid peroxidation and reduction in glutathione,superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase and glucose-6-phosphate dehydrogenase were found after ATDs exposure.Administration of P.daemia extract maintained serum biochemical indices as well as antioxidant status similar to control and diminished oxidative stress in dose dependent manner.Histological and ultra-structural observations substantiated biochemical findings.Conclusions:P.daemia has therapeutic potential against ATDs induced liver injury and may be of clinical significance after extensive studies.展开更多
Objective:To investigate the combined therapeutic potential of Gymnema(G.)sylvestre and Pergularia(P.)daemia on letrozole-induced polycystic ovarian syndrome(PCOS)in rats.Methods:Thirty six healthy female Wistar rats ...Objective:To investigate the combined therapeutic potential of Gymnema(G.)sylvestre and Pergularia(P.)daemia on letrozole-induced polycystic ovarian syndrome(PCOS)in rats.Methods:Thirty six healthy female Wistar rats with regular estrus cycles were randomly divided into six groups each of 6.GroupⅠreceived 1 mL of 0.5%carboxyl methyl cellulose orally and served as the vehicle control group,while groupsⅡtoⅥwere treated with letrozole(1 mg/kg body weight p.o.)for 21 days to induce PCOS.After induction of PCOS,groupⅡserved as the PCOS control group,without treatment;groupⅢreceived metformin(20 mg/kg body weight p.o.)as the standard group,and groupsⅣtoⅥreceived G.sylvestre(100 mg/kg body weight p.o.),P.daemia(300 mg/kg body weight p.o.),and the combination of G.sylvestre and P.daemia,respectively,for 28 days.Vaginal smears were collected from all rats daily throughout the study to determine the phases of the estrus cycle.After completing the treatment schedule,oral glucose tolerance test,serum lipid profile and reproductive hormonal analysis were carried out.Subsequently,the rats were sacrificed to collect ovary and uterus for histopathological examination.Results:The PCOS control rats showed a significant irregularity in the estrus cycle,hyperglycemia,and the altered serum lipid profile such as the increased low and very low density lipoprotein,triglyceride,and decreased high density lipoproteins.In addition,the PCOS control rats showed a significant increase in serum luteinizing hormone,testosterone,and estrogen,and decrease in follicle stimulating hormone and progesterone.These changes were significantly revoked in all the treatment groups.The test drugs also significantly reduced the gained ovary weight(P<0.001),and histopathology of the ovary showed almost normal ovary.Among the treatment groups,the group of combination treatment of G.sylvestre and P.daemia showed superior ameliorative results in PCOS parameters.Conclusions:Combination of G.sylvestre and P.daemia presents potent synergistic activity against hyperandrogenism,hyperinsulinemia,anovulation and follicular cysts in letrozole-induced PCOS rats.展开更多
Dear Editor： Pergularia daemia Forsk （Asclepiadaceae） is a perennial twining herb commonly known as veliparuthi in Tamil. The plant has anthelmintic, laxative, antidiabetic, hepatoprotective and anti-inflammatory ...Dear Editor： Pergularia daemia Forsk （Asclepiadaceae） is a perennial twining herb commonly known as veliparuthi in Tamil. The plant has anthelmintic, laxative, antidiabetic, hepatoprotective and anti-inflammatory activities. The pharmacological properties of this plant come from bioactive phytochemicals such as alkaloids, triterpenes, saponins and flavonoids. Phytochemically, the plant has been investigated for the presence of cardenolides, alkaloids, saponins and steroidal compoundst. In the present study, we developed a rapid method for identification and quantitative determination of putative phyto compounds in the crude extracts of ethyl acetate and methanol from whole plant of Pergularia daemia.展开更多
<b><span style="font-family:;" "="">Introduction<span>:</span></span></b><span><i><span style="font-family:;" "=""&g...<b><span style="font-family:;" "="">Introduction<span>:</span></span></b><span><i><span style="font-family:;" "=""> Pergularia tomentosa</span></i></span><span><span style="font-family:;" "=""> is a saharan spontaneous plant belonging to <i>Asclepiadacea </i>family. It has several medicinal properties and uses in the human food as an auxiliary in cheese-making. The purpose of this study is the extraction of the coagulant enzymatic system (C.E.S) from the plant dried leaves and its use in the preparation of fresh cheese. <b>Materials & Methods</b></span></span><span><b><span style="font-family:;" "="">: </span></b></span><span><span style="font-family:;" "="">The determination of the coagulant activity was carried out on a skimmed</span></span><span><span style="font-family:;" "=""> milk <span>powder by Berridge’s method. Cow’s milk used for coagulant strength</span> deter<span>mination and cheese-making. Proteolytic activity</span></span></span><span><span style="font-family:;" "="">. </span></span><span><span style="font-family:;" "="">The chemical </span></span><span><span style="font-family:;" "="">determinations <span>of raw milk were carried out according to AFNOR analysis methods. A</span> descriptive sensory evaluation was performed to determine the organoleptic pro<span>perties of the produced cheese. The proteolytic activity was determined b</span>y measuring the aspartyl-protease units. <b>Results:</b> The main results of the phy<span>sico-chemical characterization show that its coagulant activity (R.U mL<sup>-1</sup>)</span> was 97.19 ± 4.67, coagulant strength (SU) was 230.05 ± 0.37, proteolytic activity (μg<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">·</span>mL<sup>-1</sup>) was 0.33 ± 0.04 and coagulant activity/proteolytic activity ratio was <span>294.51. For the cheese made, it has presented the following results</span></span></span><span><span style="font-family:;" "=""> </span></span><span><span style="font-family:;" "="">as %</span></span><span><span style="font-family:;" "=""> (g/100g): <span>titrable acidity 0.80 ± 0.005, total dry extract 30.48 ± 1.490, fat 2</span></span></span><span><span style="font-family:;" "="">.</span></span><span><span style="font-family:;" "="">32 ± 0.02, fresh </span></span><span><span style="font-family:;" "="">yield 11.5 ± 0</span></span><span><span style="font-family:;" "="">.</span></span><span><span style="font-family:;" "="">09, dry yield 30.06 ± 0.05. Moreover, sensory analysis results<span> showed that fresh cheese prepared from <i>Pergularia tomentosa</i> dried</span> <span>leaves diluted enzymatic extract filtrate was characterized by whitish color</span><span>, smooth texture, with acceptable and satisfying lactic taste. <b>Conclusion:</b> The plant enzymatic system studied could be added to the list of plant rennet substitutes and, thus, contribute</span></span></span><span><span style="font-family:;" "="">s</span></span><span><span style="font-family:;" "=""> to the manufacture of enzymatic coagula</span></span><span><span style="font-family:;" "="">tion cheese.</span></span><span></span><span style="font-family:;" "=""></span>展开更多
文摘The migratory locust Locusta migratoria(Orthoptera,Acrididae)is one of the most important pests due to its extensive and severe damage to crops in large parts of Africa and Asia.Biodegradable and ecologically natural products such as bota nical insecticides are emergi ng can didates for replaceme nt of usually applied chemical pesticides.The crude methanolic extract of Pergularia tomentosa(PME)was investigated for their toxicity and physiological aspects on L.migratoria nymphs.Results showed that treatment of newly emerged fourth and fifth instar nymphs resulted in significant mortality and significant repellent activity with an LC50 value of 0.18 and 0.38%,respectively,after seven days of treatment.The PME toxicity was also dem on strated by histopathological cha nges in the alime ntary canal resulting in con siderable disorganization and severe damage of the caeca and proventriculus structure.The extract induced cellular immune reactions which manifested by a significant decrease in the number of the differential haemocyte counts(prohemocytes and plasmatocytes)and important cell lysis.Data of biochemical analyses showed that the PME reduced the activity of acetylcholinesterase and induced the glutathione S-transferases.The neurotoxic effect was confirmed by the histological alterations in the brain structure,particularly in the neurosecretory cells showing typical signs of cell necrosis.
基金Financial assistance from University Grants Commission,New Delhi(UGC-MRP,F.42-520/2013 SR)Guru Ghasidas University,Bilaspur for providing laboratory facility
文摘Objective:To evaluate therapeutic potential of hydroethanolic extract of Pergularia daemia(P.daemia) against anti-tuberculosis drugs(ATDs) induced liver injury.Methods:Wistar albino rats were divided into seven groups of six animal in each.The ATDs and P.daemia extract(100,200 and 400 mg/kg,p.o.) were conjointly administered for 8 weeks and various biochemical,histoarchitectural,ultrastructural studies were performed.Results:Administration of ATDs significantly increased aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,triglycerides,cholesterol,bilirubin and decreased glucose and albumin level.Increased lipid peroxidation and reduction in glutathione,superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase and glucose-6-phosphate dehydrogenase were found after ATDs exposure.Administration of P.daemia extract maintained serum biochemical indices as well as antioxidant status similar to control and diminished oxidative stress in dose dependent manner.Histological and ultra-structural observations substantiated biochemical findings.Conclusions:P.daemia has therapeutic potential against ATDs induced liver injury and may be of clinical significance after extensive studies.
基金The authors are acknowledged to the Chancellor,Vinayaka Mission’s Research Foundation(Deemed to be University),Salem for providing necessary support for writing this article.
文摘Objective:To investigate the combined therapeutic potential of Gymnema(G.)sylvestre and Pergularia(P.)daemia on letrozole-induced polycystic ovarian syndrome(PCOS)in rats.Methods:Thirty six healthy female Wistar rats with regular estrus cycles were randomly divided into six groups each of 6.GroupⅠreceived 1 mL of 0.5%carboxyl methyl cellulose orally and served as the vehicle control group,while groupsⅡtoⅥwere treated with letrozole(1 mg/kg body weight p.o.)for 21 days to induce PCOS.After induction of PCOS,groupⅡserved as the PCOS control group,without treatment;groupⅢreceived metformin(20 mg/kg body weight p.o.)as the standard group,and groupsⅣtoⅥreceived G.sylvestre(100 mg/kg body weight p.o.),P.daemia(300 mg/kg body weight p.o.),and the combination of G.sylvestre and P.daemia,respectively,for 28 days.Vaginal smears were collected from all rats daily throughout the study to determine the phases of the estrus cycle.After completing the treatment schedule,oral glucose tolerance test,serum lipid profile and reproductive hormonal analysis were carried out.Subsequently,the rats were sacrificed to collect ovary and uterus for histopathological examination.Results:The PCOS control rats showed a significant irregularity in the estrus cycle,hyperglycemia,and the altered serum lipid profile such as the increased low and very low density lipoprotein,triglyceride,and decreased high density lipoproteins.In addition,the PCOS control rats showed a significant increase in serum luteinizing hormone,testosterone,and estrogen,and decrease in follicle stimulating hormone and progesterone.These changes were significantly revoked in all the treatment groups.The test drugs also significantly reduced the gained ovary weight(P<0.001),and histopathology of the ovary showed almost normal ovary.Among the treatment groups,the group of combination treatment of G.sylvestre and P.daemia showed superior ameliorative results in PCOS parameters.Conclusions:Combination of G.sylvestre and P.daemia presents potent synergistic activity against hyperandrogenism,hyperinsulinemia,anovulation and follicular cysts in letrozole-induced PCOS rats.
文摘Dear Editor： Pergularia daemia Forsk （Asclepiadaceae） is a perennial twining herb commonly known as veliparuthi in Tamil. The plant has anthelmintic, laxative, antidiabetic, hepatoprotective and anti-inflammatory activities. The pharmacological properties of this plant come from bioactive phytochemicals such as alkaloids, triterpenes, saponins and flavonoids. Phytochemically, the plant has been investigated for the presence of cardenolides, alkaloids, saponins and steroidal compoundst. In the present study, we developed a rapid method for identification and quantitative determination of putative phyto compounds in the crude extracts of ethyl acetate and methanol from whole plant of Pergularia daemia.
文摘<b><span style="font-family:;" "="">Introduction<span>:</span></span></b><span><i><span style="font-family:;" "=""> Pergularia tomentosa</span></i></span><span><span style="font-family:;" "=""> is a saharan spontaneous plant belonging to <i>Asclepiadacea </i>family. It has several medicinal properties and uses in the human food as an auxiliary in cheese-making. The purpose of this study is the extraction of the coagulant enzymatic system (C.E.S) from the plant dried leaves and its use in the preparation of fresh cheese. <b>Materials & Methods</b></span></span><span><b><span style="font-family:;" "="">: </span></b></span><span><span style="font-family:;" "="">The determination of the coagulant activity was carried out on a skimmed</span></span><span><span style="font-family:;" "=""> milk <span>powder by Berridge’s method. Cow’s milk used for coagulant strength</span> deter<span>mination and cheese-making. Proteolytic activity</span></span></span><span><span style="font-family:;" "="">. </span></span><span><span style="font-family:;" "="">The chemical </span></span><span><span style="font-family:;" "="">determinations <span>of raw milk were carried out according to AFNOR analysis methods. A</span> descriptive sensory evaluation was performed to determine the organoleptic pro<span>perties of the produced cheese. The proteolytic activity was determined b</span>y measuring the aspartyl-protease units. <b>Results:</b> The main results of the phy<span>sico-chemical characterization show that its coagulant activity (R.U mL<sup>-1</sup>)</span> was 97.19 ± 4.67, coagulant strength (SU) was 230.05 ± 0.37, proteolytic activity (μg<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">·</span>mL<sup>-1</sup>) was 0.33 ± 0.04 and coagulant activity/proteolytic activity ratio was <span>294.51. For the cheese made, it has presented the following results</span></span></span><span><span style="font-family:;" "=""> </span></span><span><span style="font-family:;" "="">as %</span></span><span><span style="font-family:;" "=""> (g/100g): <span>titrable acidity 0.80 ± 0.005, total dry extract 30.48 ± 1.490, fat 2</span></span></span><span><span style="font-family:;" "="">.</span></span><span><span style="font-family:;" "="">32 ± 0.02, fresh </span></span><span><span style="font-family:;" "="">yield 11.5 ± 0</span></span><span><span style="font-family:;" "="">.</span></span><span><span style="font-family:;" "="">09, dry yield 30.06 ± 0.05. Moreover, sensory analysis results<span> showed that fresh cheese prepared from <i>Pergularia tomentosa</i> dried</span> <span>leaves diluted enzymatic extract filtrate was characterized by whitish color</span><span>, smooth texture, with acceptable and satisfying lactic taste. <b>Conclusion:</b> The plant enzymatic system studied could be added to the list of plant rennet substitutes and, thus, contribute</span></span></span><span><span style="font-family:;" "="">s</span></span><span><span style="font-family:;" "=""> to the manufacture of enzymatic coagula</span></span><span><span style="font-family:;" "="">tion cheese.</span></span><span></span><span style="font-family:;" "=""></span>
