The two-layer cold storage method (TLM) was f irst reported in 1988, consisting of a perfluorochemical (PFC) and initially Euro-Collins' solution, which was later replaced by University of Wisconsin solution (UW)....The two-layer cold storage method (TLM) was f irst reported in 1988, consisting of a perfluorochemical (PFC) and initially Euro-Collins' solution, which was later replaced by University of Wisconsin solution (UW). PFC is a biologically inert liquid and acts as an oxygen-supplying agent. A pancreas preserved using the TLM is oxygenated through the PFC and substrates are supplied by the UW solution. This allows the pancreas preserved using the TLM to generate adenosine triphosphate during storage, prolonging the preservation time. In a canine model, the TLM was shown to repair and resuscitate warm ischemically damaged pancreata during preservation, improve pancreas graft survival after transplantation, and also improve the islet yield after isolation. Clinical trials using the TLM in pancreas preservation before whole-pancreas transplantation and islet isolation have shown promising outcomes. We describe the role of the TLM in pancreas and islet transplantation.展开更多
The present study was undertaken to determine whether perfluorochemical (PFC)would be useful for long-term rat(Lewis)pancreas preservation.Rat pancreases werestored at 4℃ in Euro-Collin'(E-C) solution, UW solutio...The present study was undertaken to determine whether perfluorochemical (PFC)would be useful for long-term rat(Lewis)pancreas preservation.Rat pancreases werestored at 4℃ in Euro-Collin'(E-C) solution, UW solution or PFC solution solution for 24 h,48h and 72h prior to heterotopic transplantation into syngeneic rats withstretozotocin-induced diabetes,Functional graft success rates following 24h,48h and72h simple cold storage were 60% (3/5),20%(1/5)and 0%(0/5),respectively,in theE-C group,100% (5/5),44%(4/9)and 0%(0/8),respectively,in the UW group and100%(5/5),83%(5/6)and 67%(4/7),respectively,in the PFC group.A significanthigher 2-week success rate was seen in PFC group after 72h cold preservation,as com-pared with that in E-C and UW groups (P<0.05 and P<0.01).The importance ofoxygenation of grafts in PFC during cold preservation has also been investgated.展开更多
Perfluorochemicals(PFCs)are emergent and persistent organic pollutants with widespread application.Their structural similarity and detection in serum raises substantial concerns regarding their toxicological effects.W...Perfluorochemicals(PFCs)are emergent and persistent organic pollutants with widespread application.Their structural similarity and detection in serum raises substantial concerns regarding their toxicological effects.While the endocrine-disrupting effects of PFCs are well-recognized,the structure−activity relationship with respect to vascular function has not been investigated yet.This study addresses this critical gap by investigating the impact of PFCs on endothelial cell function,a key determinant of cardiovascular health.Through a machine learning-based quantitative structure−activity relationship(QSAR)model,we analyzed 16 structural descriptors for 23 environ-mentally prevalent PFCs with respect to their binding affinities to endothelial cell receptors.The eXtreme Gradient Boosting(XGBoost)algorithm suggested short-chain PFCs with strong acid groups may as particularly detrimental to endothelial cells,a finding substantiated by subsequent cell culture experiments.We also integrated computational and experimental approaches,providing a detailed understanding of the structure−activity and dose−response relationships of PFCs.Furthermore,the population health risk assessment,linking in vitro adverse effect with in vivo exposure data,indicated differences in risks across countries due to the global shift in the fluoride industry;the entire Chinese population is at high risk,with risk varying by gender and industrialization level.This study not only elucidates the structure−activity relationship of PFCs on vascular function but also offers a strategic framework for managing toxic PFCs and proposing the development of safer alternatives.展开更多
文摘The two-layer cold storage method (TLM) was f irst reported in 1988, consisting of a perfluorochemical (PFC) and initially Euro-Collins' solution, which was later replaced by University of Wisconsin solution (UW). PFC is a biologically inert liquid and acts as an oxygen-supplying agent. A pancreas preserved using the TLM is oxygenated through the PFC and substrates are supplied by the UW solution. This allows the pancreas preserved using the TLM to generate adenosine triphosphate during storage, prolonging the preservation time. In a canine model, the TLM was shown to repair and resuscitate warm ischemically damaged pancreata during preservation, improve pancreas graft survival after transplantation, and also improve the islet yield after isolation. Clinical trials using the TLM in pancreas preservation before whole-pancreas transplantation and islet isolation have shown promising outcomes. We describe the role of the TLM in pancreas and islet transplantation.
文摘The present study was undertaken to determine whether perfluorochemical (PFC)would be useful for long-term rat(Lewis)pancreas preservation.Rat pancreases werestored at 4℃ in Euro-Collin'(E-C) solution, UW solution or PFC solution solution for 24 h,48h and 72h prior to heterotopic transplantation into syngeneic rats withstretozotocin-induced diabetes,Functional graft success rates following 24h,48h and72h simple cold storage were 60% (3/5),20%(1/5)and 0%(0/5),respectively,in theE-C group,100% (5/5),44%(4/9)and 0%(0/8),respectively,in the UW group and100%(5/5),83%(5/6)and 67%(4/7),respectively,in the PFC group.A significanthigher 2-week success rate was seen in PFC group after 72h cold preservation,as com-pared with that in E-C and UW groups (P<0.05 and P<0.01).The importance ofoxygenation of grafts in PFC during cold preservation has also been investgated.
基金supported by the National Natural Science Foundation of China(82241086,22222610,82473674)Qingdao Natural Science Foundation(24-4-4-zrjj-157-jch).
文摘Perfluorochemicals(PFCs)are emergent and persistent organic pollutants with widespread application.Their structural similarity and detection in serum raises substantial concerns regarding their toxicological effects.While the endocrine-disrupting effects of PFCs are well-recognized,the structure−activity relationship with respect to vascular function has not been investigated yet.This study addresses this critical gap by investigating the impact of PFCs on endothelial cell function,a key determinant of cardiovascular health.Through a machine learning-based quantitative structure−activity relationship(QSAR)model,we analyzed 16 structural descriptors for 23 environ-mentally prevalent PFCs with respect to their binding affinities to endothelial cell receptors.The eXtreme Gradient Boosting(XGBoost)algorithm suggested short-chain PFCs with strong acid groups may as particularly detrimental to endothelial cells,a finding substantiated by subsequent cell culture experiments.We also integrated computational and experimental approaches,providing a detailed understanding of the structure−activity and dose−response relationships of PFCs.Furthermore,the population health risk assessment,linking in vitro adverse effect with in vivo exposure data,indicated differences in risks across countries due to the global shift in the fluoride industry;the entire Chinese population is at high risk,with risk varying by gender and industrialization level.This study not only elucidates the structure−activity relationship of PFCs on vascular function but also offers a strategic framework for managing toxic PFCs and proposing the development of safer alternatives.
