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Embryonic and Postembryonic Development of Percocypris pingi pingi( Tchang)
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作者 Jiansheng LAI Jun DU +3 位作者 Gang ZHAO Xiaochuan DENG Hua LI Xingheng HE 《Agricultural Biotechnology》 CAS 2014年第6期51-56,共6页
This study aimed to investigate the morphological characteristics of Percocypris pingi pingi (Tchang) at the embryonic and postembryonic developmental periods and to demonstrate the early developmental stage of P. p... This study aimed to investigate the morphological characteristics of Percocypris pingi pingi (Tchang) at the embryonic and postembryonic developmental periods and to demonstrate the early developmental stage of P. pingi pingi (Tchang). According to the results, the fully mature eggs were spherical, orange-col- ored, sticky, 2.2 mm in diameter; the maximum outer diameter reached 3.2 -3.8 mm after absorbing water. When water temperature was (18 -+ 0.5) ~C, the first cleavage occun~ at 2h 28 min post-fertilization; organs began to form at 45 h 23 min post-fertilization; larvae were hatched at 126 h 28 min post-fertilization. Newly hatched larvae exhibited a total length of 10.4 mm, with large and compressed yolk sacs. According to the morphological characteristics, the embryonic de- velopmental process of P. pingi pingi (Tchang) could be divided into seven continuous stages, including fertilized egg stage, cleavage stage, blastula stage, early gastrula stage, neurala stage, organ formation stage and hatching stage, which were further divided into 32 phases. When water temperature ranged from 18.8 ~C to 25.0 ~C, the whole developmental process of P. pingi pingi (Tchang) from larvae to juveniles lasted 93 d. According to the morphological and ecological charac- teristics, the pestembryonic developmental process of P. pingi pingi (Tchang) could be divided into three stages, including yolk-sac larva stage, late larva stage and junenile stage. This study laid solid foundation for . 展开更多
关键词 percocypris pingi pingi (Tchang) Fertilized eggs Embryonic development Postembryonic development
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金沙鲈鲤全长转录组测序分析与抗菌肽基因的鉴定
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作者 朱思艺 邓龙君 +4 位作者 李天才 张扬 郭勇垚 罗伟 杜宗君 《湖北农业科学》 2025年第3期167-175,共9页
为进一步丰富金沙鲈鲤(Percocypris pingi)基因信息数据库,采用PacBio测序平台对金沙鲈鲤进行全长转录组测序。共获得499 007个全长非嵌合序列(Full-length non-chimeric read,FLNC),通过聚类和去冗余获得70 321个isoforms,isoforms的... 为进一步丰富金沙鲈鲤(Percocypris pingi)基因信息数据库,采用PacBio测序平台对金沙鲈鲤进行全长转录组测序。共获得499 007个全长非嵌合序列(Full-length non-chimeric read,FLNC),通过聚类和去冗余获得70 321个isoforms,isoforms的平均长度和N50分别为1 882 bp、2 243 bp。将isoforms与NR、GO、KEGG、KOG和Swiss-Prot 5个公共数据库进行比对,成功注释了57 521个isoforms,并鉴定到2 310个TF、16 225个LncRNA和35 314条CDS。鉴定到5个抗菌肽基因,包括2个hepcidin基因和3个hemoglobin基因。金沙鲈鲤的hepcidin-1、hepcidin-2与多鳞白甲鱼(Onychostoma macrolepis)的hepcidin亲缘关系较近;金沙鲈鲤的hemoglobin-1α与斑马鱼(Danio rerio)的hemoglobin α亲缘关系较近;金沙鲈鲤的hemoglobin-1β与斑点叉尾鮰(Ictalurus punctatus)的hemoglobin 1β、hemoglobin 2β亲缘关系较近;hemoglobin-2β与多鳞白甲鱼的hemoglobin 2β亲缘关系较近。通过对金沙鲈鲤全长转录组的注释分析,阐明了金沙鲈鲤基因参与的生物过程、所在的代谢途径或信号通路等,可以更有效地探究抗菌肽在鱼类免疫中的作用。 展开更多
关键词 金沙鲈鲤(percocypris pingi) 全长转录组 测序 抗菌肽基因 鉴定
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