BACKGROUND The unique survival environment of Helicobacter pylori(H.pylori)presents challenges in establishing appropriate in vitro culture conditions.The fastidious nature of H.pylori and its relatively low growth ra...BACKGROUND The unique survival environment of Helicobacter pylori(H.pylori)presents challenges in establishing appropriate in vitro culture conditions.The fastidious nature of H.pylori and its relatively low growth rate emphasize the need for customized culture conditions,which are essential for both research and clinical applications.AIM To identify the optimal liquid culture medium for H.pylori using both reference and clinical strains.METHODS Nine H.pylori strains were incubated in 10 different broth media commonly used for bacterial cultures under microaerophilic conditions.Bacterial concentrations were estimated using serial dilutions and the pour plate method.A range of H.pylori initial inoculum concentrations was tested for each strain.For growth evaluation,optical density at 600 nm was measured at 24,48,and 72 hours.The growth trends of H.pylori were compared among strains and media.RESULTS All H.pylori strains grew successfully over time,regardless of the initial inoculum concentration.The specific growth trends and the broth that yielded the highest optical density value at each time point varied with strain.The reference strains(H.pylori 26695,ATCC 43504,and J99)consistently exhibited robust growth,whereas the clinical strains displayed distinct media preferences.Overall,bacterial growth was higher in chopped meat carbohydrate broth,Columbia broth,and fastidious anaerobe broth than in Brucella broth after 72 hours.CONCLUSION Chopped meat carbohydrate broth was the most effective for H.pylori liquid culture,and Columbia broth and fastidious anaerobe broth also supported greater growth than Brucella broth.展开更多
Objective:To investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour.Methods:Defatted process.inoculums preparation,evaluation of bacterial growth,preparation of cooked a...Objective:To investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour.Methods:Defatted process.inoculums preparation,evaluation of bacterial growth,preparation of cooked and hydrolyzed media and growth turbidity of tested bacteria were determined.Results:Two percent defatted flour was found to be suitable concentration for the growth of pathogenic bacteria:Escherichia coli(ATCC 25922)(E,coli),Pseudomonas aeruginosa(ATCC27853),Salmonella(NCTC 8385)and Shigella flexneri(ATCC 12022)(S.flexneri),while 3%defatted flour was suitable for Staphylococcus aureus(ATCC 25923)(S.aureus).E.coli(93±1)and S.flexneri(524±1)colony count were significantly(P≤0.05)greater in defatted flour without supplement than in supplemented medium.E.coli[(3.72×10~9±2)CFU/mL],S.aureus[(7.4×10~9±2)CFU/mL],S.flexneri[(4.03×10~9±2)CFU/mL]and Salmonella[(2.37×10~9±1)CFU/mL]in non-hydrolyzed sample were statistically(P≤0.05)greater than hydrolyzed one and commercial peptone agar.Colony count of Salmonella[(4.55≤10~9±3)CFU/mL],S.flexneri[(5.40≤10~9±3)CFU/mL]and Lyesria moncytogenes(ATCC 19116)[(5.4×10~9±3)CFU/mL]on raw defatted flour agar was significantly(P≤0.05)greater than cooked defatted flour and commercial peptone agar.Biomass of E.coli,S.aureus.Salmonella and Enterococcus faecalis in non-hydrolyzed defatted flour is highly increased over hydrolyzed defatted flour and commercial peptone broth.Conclusions:The defatted flour agar was found to be better microbial media or comparable with peptone agar.The substances in it can serve as sources of carbon,nitrogen,vitamins and minerals that are essential to support the growth of microorganisms without any supplements.Currently,all supplements of peptone agar are very expensive in the market.展开更多
The experiments were carried out at the Post Graduate Research Center, to study the influence of Gibberellic Acid (50 ppm) and Oxygenated Peptone (1% aqueous solution) on chick pea (Cicer arietinum L. cv. Vijay) durin...The experiments were carried out at the Post Graduate Research Center, to study the influence of Gibberellic Acid (50 ppm) and Oxygenated Peptone (1% aqueous solution) on chick pea (Cicer arietinum L. cv. Vijay) during germination by giving pre-sowing soaking treatment for 6 hours using petriplate method. Both the treatments enhanced the germination process. GA treatment was useful to increase shoot length, mobilization efficiency, emergence index, speed of germination and co-efficient of germination while oxygenated peptone showed an upper hand in root length, shoot/root ratio, biomass and vigour index. GA led to comparatively more synthesis of nucleic acids while oxygenated peptone showed more increase in total carbohydrates and soluble protein content. However, the activity of enzymes like amylase, catalase and protease showed upper hand with oxygenated peptone as compared to GA. In fact GA is costlier and can not be used in organic farming as it enters metabolic pathways of plant and alters them. Hence the use of oxygenated peptone is recommended being less expensive and usable under organic farming condition as it does not enter the plant metabolic pathways and yet brings about significant positive effect.展开更多
This study is aiming at investigating the metabolic characteristics of Ethanoligenens Harbinense YUAN-3.The effects of yeast extract powder and supplemental vitamins on the growth medium were assessed.With yeast powde...This study is aiming at investigating the metabolic characteristics of Ethanoligenens Harbinense YUAN-3.The effects of yeast extract powder and supplemental vitamins on the growth medium were assessed.With yeast powder as the sole nitrogen source,the biomass yield and hydrogen-producing capacity of YUAN-3 from glucose fermentation significantly increased.Adding vitamin in yeast powder can enhance hydrogen yield compared with no adding.Therefore vitamin supplement can be considered as an effective growth factor in this study.Batch culture also produced larger yields of hydrogen.展开更多
基金Supported by the Bio&Medical Technology Development Program of the National Research Foundation Funded by the Korean Government(MSIT),No.RS-2023-00223501Hallym University Research Fund.
文摘BACKGROUND The unique survival environment of Helicobacter pylori(H.pylori)presents challenges in establishing appropriate in vitro culture conditions.The fastidious nature of H.pylori and its relatively low growth rate emphasize the need for customized culture conditions,which are essential for both research and clinical applications.AIM To identify the optimal liquid culture medium for H.pylori using both reference and clinical strains.METHODS Nine H.pylori strains were incubated in 10 different broth media commonly used for bacterial cultures under microaerophilic conditions.Bacterial concentrations were estimated using serial dilutions and the pour plate method.A range of H.pylori initial inoculum concentrations was tested for each strain.For growth evaluation,optical density at 600 nm was measured at 24,48,and 72 hours.The growth trends of H.pylori were compared among strains and media.RESULTS All H.pylori strains grew successfully over time,regardless of the initial inoculum concentration.The specific growth trends and the broth that yielded the highest optical density value at each time point varied with strain.The reference strains(H.pylori 26695,ATCC 43504,and J99)consistently exhibited robust growth,whereas the clinical strains displayed distinct media preferences.Overall,bacterial growth was higher in chopped meat carbohydrate broth,Columbia broth,and fastidious anaerobe broth than in Brucella broth after 72 hours.CONCLUSION Chopped meat carbohydrate broth was the most effective for H.pylori liquid culture,and Columbia broth and fastidious anaerobe broth also supported greater growth than Brucella broth.
基金supported by the University of Gondar:General Director.Research & Community Service Core Process,Grant NO:UOG/Budget/no.6215
文摘Objective:To investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour.Methods:Defatted process.inoculums preparation,evaluation of bacterial growth,preparation of cooked and hydrolyzed media and growth turbidity of tested bacteria were determined.Results:Two percent defatted flour was found to be suitable concentration for the growth of pathogenic bacteria:Escherichia coli(ATCC 25922)(E,coli),Pseudomonas aeruginosa(ATCC27853),Salmonella(NCTC 8385)and Shigella flexneri(ATCC 12022)(S.flexneri),while 3%defatted flour was suitable for Staphylococcus aureus(ATCC 25923)(S.aureus).E.coli(93±1)and S.flexneri(524±1)colony count were significantly(P≤0.05)greater in defatted flour without supplement than in supplemented medium.E.coli[(3.72×10~9±2)CFU/mL],S.aureus[(7.4×10~9±2)CFU/mL],S.flexneri[(4.03×10~9±2)CFU/mL]and Salmonella[(2.37×10~9±1)CFU/mL]in non-hydrolyzed sample were statistically(P≤0.05)greater than hydrolyzed one and commercial peptone agar.Colony count of Salmonella[(4.55≤10~9±3)CFU/mL],S.flexneri[(5.40≤10~9±3)CFU/mL]and Lyesria moncytogenes(ATCC 19116)[(5.4×10~9±3)CFU/mL]on raw defatted flour agar was significantly(P≤0.05)greater than cooked defatted flour and commercial peptone agar.Biomass of E.coli,S.aureus.Salmonella and Enterococcus faecalis in non-hydrolyzed defatted flour is highly increased over hydrolyzed defatted flour and commercial peptone broth.Conclusions:The defatted flour agar was found to be better microbial media or comparable with peptone agar.The substances in it can serve as sources of carbon,nitrogen,vitamins and minerals that are essential to support the growth of microorganisms without any supplements.Currently,all supplements of peptone agar are very expensive in the market.
文摘The experiments were carried out at the Post Graduate Research Center, to study the influence of Gibberellic Acid (50 ppm) and Oxygenated Peptone (1% aqueous solution) on chick pea (Cicer arietinum L. cv. Vijay) during germination by giving pre-sowing soaking treatment for 6 hours using petriplate method. Both the treatments enhanced the germination process. GA treatment was useful to increase shoot length, mobilization efficiency, emergence index, speed of germination and co-efficient of germination while oxygenated peptone showed an upper hand in root length, shoot/root ratio, biomass and vigour index. GA led to comparatively more synthesis of nucleic acids while oxygenated peptone showed more increase in total carbohydrates and soluble protein content. However, the activity of enzymes like amylase, catalase and protease showed upper hand with oxygenated peptone as compared to GA. In fact GA is costlier and can not be used in organic farming as it enters metabolic pathways of plant and alters them. Hence the use of oxygenated peptone is recommended being less expensive and usable under organic farming condition as it does not enter the plant metabolic pathways and yet brings about significant positive effect.
基金Sponsored by the National Natural Science Foundation of China(Grant No. 30870037)the Major Program of National Natural Science Foundation of China(Grant No. 50638020)+1 种基金the National Science Foundation for Post-doctoral Scientists of China(Grant No.AUGA41309045)the Heilongjiang Postdoctoral Grant(Grant No.AUGA41100165)
文摘This study is aiming at investigating the metabolic characteristics of Ethanoligenens Harbinense YUAN-3.The effects of yeast extract powder and supplemental vitamins on the growth medium were assessed.With yeast powder as the sole nitrogen source,the biomass yield and hydrogen-producing capacity of YUAN-3 from glucose fermentation significantly increased.Adding vitamin in yeast powder can enhance hydrogen yield compared with no adding.Therefore vitamin supplement can be considered as an effective growth factor in this study.Batch culture also produced larger yields of hydrogen.
