Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic ...Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic strains with similar characters to that of natural infectious cases.The strains Lb18-01 and Lb18-02 showed strong pathogenicity to healthy P.sinensis in the artificial infection experiment,so they were the pathogenic strain of the disease.According to the morphology,physicochemical characteristics,16 S rDNA sequence analysis and phylogenetic tree clustering,the pathogenic strains Lb18-01 and Lb18-02 were identified as Proteus vulgaris and Chryseobacterium meningosepticum which were tolerant to 20 drugs such as penicillin,tetracycline and ampicillin.Histopathological observation on diseased P.sinensis showed the pathological symptoms of sepsis such as hemorrhage and congestion of liver,spleen and intestine,and glomerular disintegration.展开更多
基金Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals(2018TP1027)Key R&D Projects of Hunan Province(2018NK2074).
文摘Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic strains with similar characters to that of natural infectious cases.The strains Lb18-01 and Lb18-02 showed strong pathogenicity to healthy P.sinensis in the artificial infection experiment,so they were the pathogenic strain of the disease.According to the morphology,physicochemical characteristics,16 S rDNA sequence analysis and phylogenetic tree clustering,the pathogenic strains Lb18-01 and Lb18-02 were identified as Proteus vulgaris and Chryseobacterium meningosepticum which were tolerant to 20 drugs such as penicillin,tetracycline and ampicillin.Histopathological observation on diseased P.sinensis showed the pathological symptoms of sepsis such as hemorrhage and congestion of liver,spleen and intestine,and glomerular disintegration.
