<span style="font-family:Verdana;">Phytochemical investigation of the </span><span style="font-family:Verdana;">seeds of </span><i><span style="font-family:Ver...<span style="font-family:Verdana;">Phytochemical investigation of the </span><span style="font-family:Verdana;">seeds of </span><i><span style="font-family:Verdana;">Pentaclethra </span><span style="font-family:Verdana;">macrophylla</span> </i><span style="font-family:Verdana;">led to the isolation of a mixture of two new aromatic monoterpene glycosides, </span><span style="font-family:Verdana;">pentamacrophylloside</span><span style="font-family:Verdana;"> A (</span><b><span style="font-family:Verdana;">1a</span></b><span style="font-family:Verdana;">) and </span><span style="font-family:Verdana;">pentamacrophylloside</span><span style="font-family:Verdana;"> B (</span><b><span style="font-family:Verdana;">1b</span></b><span style="font-family:Verdana;">), together with </span><span><span style="font-family:Verdana;">six known secondary metabolites: Comososide (</span><b><span style="font-family:Verdana;">2</span></b><span style="font-family:Verdana;">), </span><span style="font-family:Verdana;">secopentaclethroside</span><span style="font-family:Verdana;"> (</span><b><span style="font-family:Verdana;">3</span></b><span style="font-family:Verdana;">), </span><span style="font-family:Verdana;">caffeoylputrescine</span><span style="font-family:Verdana;"> (</span><b><span style="font-family:Verdana;">4</span></b><span style="font-family:Verdana;">), </span></span><i><span style="font-family:Verdana;">β</span></i><span><span style="font-family:Verdana;">-sitosterol-3-</span><i><span style="font-family:Verdana;">O</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-D-glucopyranoside (</span><b><span style="font-family:Verdana;">5</span></b><span style="font-family:Verdana;">), </span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">2-hydroxymethyl-5-(2-</span><span style="font-family:Verdana;">hydroxypropan</span><span style="font-family:Verdana;">-2-</span><span style="font-family:Verdana;">yl</span><span style="font-family:Verdana;">)phenol (</span><b><span style="font-family:Verdana;">6</span></b><span style="font-family:Verdana;">), and sucrose (</span><b><span style="font-family:Verdana;">7</span></b><span style="font-family:Verdana;">). Their structures were elucidated </span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">mainly by extensive</span><span style="font-family:Verdana;"> spectroscopic </span><span style="font-family:Verdana;">analysis </span><span style="font-family:Verdana;">(1D and 2D NMR), </span><span style="font-family:Verdana;">high-resolution </span><span><span style="font-family:Verdana;">mass spectrometry </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> by comparison of their spectral data with those of related compounds. The extracts and compounds </span><b><span style="font-family:Verdana;">3</span></b><span style="font-family:Verdana;"> and </span><b><span style="font-family:Verdana;">4</span></b><span style="font-family:Verdana;"> were screened for their antimicrobial activity. The </span></span><i><span style="font-family:Verdana;">n</span></i><span style="font-family:Verdana;">-BuOH fraction showed a weak effect against three microbial strains: </span><i><span style="font-family:Verdana;">Candida albicans</span></i><span style="font-family:Verdana;"> (MIC: 256 μg/mL), </span><i><span style="font-family:Verdana;">Enterococcus </span><span style="font-family:Verdana;">faecalis</span></i><span style="font-family:Verdana;"> (MIC: 512 μg/mL), and </span><i><span style="font-family:Verdana;">Proteus mirabilis </span></i><span style="font-family:Verdana;">(MIC: 512 μg/mL) while no significant inhibition was observed for pure compounds when compared to </span><span style="font-family:Verdana;">ketoconazole</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">ciprofloxacin</span><span style="font-family:Verdana;"> used as references. Furthermore, the ethnobotany and pharmacology of this plant are reviewed, and </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">chemophenetic</span><span style="font-family:Verdana;"> significance of the isolation of the </span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">above</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">secondary metabolites</span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> is discussed. This is the first report on the isolation of aromatic monoterpenoids from a plant of the genus </span><i><span style="font-family:Verdana;">Pentaclethra.</span></i></span></span></span>展开更多
The present study assessed the proximate composition,in vitro antioxidant activity and GC-MS analyses of fermented Pentaclethra macrophylla seeds extract(FPMSE).The in vitro antioxidant activity was assessed using 2,2...The present study assessed the proximate composition,in vitro antioxidant activity and GC-MS analyses of fermented Pentaclethra macrophylla seeds extract(FPMSE).The in vitro antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazl(DPPH)while Gas Chromatography-Mass Spectrophotometry(GC-MS)technique was used to identify the volatile compounds in FPMSE.FPMSE revealed a dose dependent percentage inhibition of the extract on DPPH assay.Proximate composition of FPMSE constitutes respectively;ash(0.33±0.12%),protein(2.19±0.08%),fats and oil(80.60±0.00%),carbohydrate(16.88±0.00%)while moisture and fibre were not detected.However,the pulverised sample constitute respectively;ash(1.27±0.58%),protein(10.51±0.60%),moisture(8.27±0.12%),fats and oil(32.33±0.12%),fibre(7.27±0.12%)and carbohydrate(40.35±0.00%).GC-MS analyses revealed fourteen(14)fatty acids including Octadecanoic acid(1.95%),Benzyloxymethylimine(43.89%),2-Allylpent-4-enoic acid benzyl ester(26.25%),Hexadecanoic acid,methyl ester(1.09%),Oleic Acid(0.25%),9,12-Octadecadienoic acid,methyl ester(Omega-6-fatty acid)(1.52%).The bioactive compounds identified in FPMSE could contribute to the pharmacological properties of P.macrophylla seeds and hence,could be of considerable interest for the development of new drugs.展开更多
文摘<span style="font-family:Verdana;">Phytochemical investigation of the </span><span style="font-family:Verdana;">seeds of </span><i><span style="font-family:Verdana;">Pentaclethra </span><span style="font-family:Verdana;">macrophylla</span> </i><span style="font-family:Verdana;">led to the isolation of a mixture of two new aromatic monoterpene glycosides, </span><span style="font-family:Verdana;">pentamacrophylloside</span><span style="font-family:Verdana;"> A (</span><b><span style="font-family:Verdana;">1a</span></b><span style="font-family:Verdana;">) and </span><span style="font-family:Verdana;">pentamacrophylloside</span><span style="font-family:Verdana;"> B (</span><b><span style="font-family:Verdana;">1b</span></b><span style="font-family:Verdana;">), together with </span><span><span style="font-family:Verdana;">six known secondary metabolites: Comososide (</span><b><span style="font-family:Verdana;">2</span></b><span style="font-family:Verdana;">), </span><span style="font-family:Verdana;">secopentaclethroside</span><span style="font-family:Verdana;"> (</span><b><span style="font-family:Verdana;">3</span></b><span style="font-family:Verdana;">), </span><span style="font-family:Verdana;">caffeoylputrescine</span><span style="font-family:Verdana;"> (</span><b><span style="font-family:Verdana;">4</span></b><span style="font-family:Verdana;">), </span></span><i><span style="font-family:Verdana;">β</span></i><span><span style="font-family:Verdana;">-sitosterol-3-</span><i><span style="font-family:Verdana;">O</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-D-glucopyranoside (</span><b><span style="font-family:Verdana;">5</span></b><span style="font-family:Verdana;">), </span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">2-hydroxymethyl-5-(2-</span><span style="font-family:Verdana;">hydroxypropan</span><span style="font-family:Verdana;">-2-</span><span style="font-family:Verdana;">yl</span><span style="font-family:Verdana;">)phenol (</span><b><span style="font-family:Verdana;">6</span></b><span style="font-family:Verdana;">), and sucrose (</span><b><span style="font-family:Verdana;">7</span></b><span style="font-family:Verdana;">). Their structures were elucidated </span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">mainly by extensive</span><span style="font-family:Verdana;"> spectroscopic </span><span style="font-family:Verdana;">analysis </span><span style="font-family:Verdana;">(1D and 2D NMR), </span><span style="font-family:Verdana;">high-resolution </span><span><span style="font-family:Verdana;">mass spectrometry </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> by comparison of their spectral data with those of related compounds. The extracts and compounds </span><b><span style="font-family:Verdana;">3</span></b><span style="font-family:Verdana;"> and </span><b><span style="font-family:Verdana;">4</span></b><span style="font-family:Verdana;"> were screened for their antimicrobial activity. The </span></span><i><span style="font-family:Verdana;">n</span></i><span style="font-family:Verdana;">-BuOH fraction showed a weak effect against three microbial strains: </span><i><span style="font-family:Verdana;">Candida albicans</span></i><span style="font-family:Verdana;"> (MIC: 256 μg/mL), </span><i><span style="font-family:Verdana;">Enterococcus </span><span style="font-family:Verdana;">faecalis</span></i><span style="font-family:Verdana;"> (MIC: 512 μg/mL), and </span><i><span style="font-family:Verdana;">Proteus mirabilis </span></i><span style="font-family:Verdana;">(MIC: 512 μg/mL) while no significant inhibition was observed for pure compounds when compared to </span><span style="font-family:Verdana;">ketoconazole</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">ciprofloxacin</span><span style="font-family:Verdana;"> used as references. Furthermore, the ethnobotany and pharmacology of this plant are reviewed, and </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">chemophenetic</span><span style="font-family:Verdana;"> significance of the isolation of the </span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">above</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">secondary metabolites</span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> is discussed. This is the first report on the isolation of aromatic monoterpenoids from a plant of the genus </span><i><span style="font-family:Verdana;">Pentaclethra.</span></i></span></span></span>
文摘The present study assessed the proximate composition,in vitro antioxidant activity and GC-MS analyses of fermented Pentaclethra macrophylla seeds extract(FPMSE).The in vitro antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazl(DPPH)while Gas Chromatography-Mass Spectrophotometry(GC-MS)technique was used to identify the volatile compounds in FPMSE.FPMSE revealed a dose dependent percentage inhibition of the extract on DPPH assay.Proximate composition of FPMSE constitutes respectively;ash(0.33±0.12%),protein(2.19±0.08%),fats and oil(80.60±0.00%),carbohydrate(16.88±0.00%)while moisture and fibre were not detected.However,the pulverised sample constitute respectively;ash(1.27±0.58%),protein(10.51±0.60%),moisture(8.27±0.12%),fats and oil(32.33±0.12%),fibre(7.27±0.12%)and carbohydrate(40.35±0.00%).GC-MS analyses revealed fourteen(14)fatty acids including Octadecanoic acid(1.95%),Benzyloxymethylimine(43.89%),2-Allylpent-4-enoic acid benzyl ester(26.25%),Hexadecanoic acid,methyl ester(1.09%),Oleic Acid(0.25%),9,12-Octadecadienoic acid,methyl ester(Omega-6-fatty acid)(1.52%).The bioactive compounds identified in FPMSE could contribute to the pharmacological properties of P.macrophylla seeds and hence,could be of considerable interest for the development of new drugs.
