Penaeus vannamei, known as the whiteleg shrimp, holds significant economic importance in aquaculture. The intensive culture of P. vannamei poses substantial environmental risks, particularly in fragile ecosystems like...Penaeus vannamei, known as the whiteleg shrimp, holds significant economic importance in aquaculture. The intensive culture of P. vannamei poses substantial environmental risks, particularly in fragile ecosystems like estuarine mangroves. Although there is a consensus on the pollution and harmful effects of microplastics(MPs), study on the pollution and potential risks posed by P. vannamei in estuary mangroves remains scarce. Therefore, the extent of microplastic pollution was evaluated and the correlation between the properties of MPs and the potential risks they pose to P. vannamei was examined.The average MP abundance in P. vannamei from Jiulong River estuary was determined to be 0.46±0.03 n/g in five different polymers, i.e., polypropylene(PP), polyethylene(PE), polystyrene(PS), polyethylene terephthalate(PET), and polyvinyl chloride resin(PVC). The total percentage of PS, PE, and PET polymers was the greatest(73.85%), where PET and PP(26.16%) were highly correlated in size and shape. Small MPs(1-200 μm) come in irregular particles, fragments, films, and large-sized MPs(200-1 000 μm) were mainly foam-shaped. The hazard risk level of MPs in P. vannamei from Jiulong River estuary reached grade Ⅲ, and the overall hazard risk index(H) value was 593.66. Among all samples,PVC polymer accounted for the lowest proportion(5.52%), but the H value was as high as 582.42, which contributed 98.11% to the overall hazard risk index. Regardless of global or Chinese, the microplastic pollution of P. vannamei in the estuary is at a moderate level. The protocol for MPs characteristics and correlation in seafood should be the basis for the risk assessment framework. Further studies are needed to evaluate how the exposure to MPs poses a risk for human health.展开更多
The relationship between microbial communities and their hosts is important to the health and productivity of marine ani-mals in aquaculture.This study investigated the microbiomes in the gut and gill of Penaeus vanna...The relationship between microbial communities and their hosts is important to the health and productivity of marine ani-mals in aquaculture.This study investigated the microbiomes in the gut and gill of Penaeus vannamei using 16S rRNA gene sequencing,comparing the microbial characteristics in the fast-growing trait and in the control group.Fast-growing individuals exhibited sig-nificantly different growth parameters and gut/gill microbiomes.Significantly higher weight gain and condition factors,as well as lower hepatosomatic indices(HSI),were observed in fast-growing individuals.Fast-growing individuals also displayed significantly higher operational taxonomic unit(OTU)counts(in gills)and greater phylogenetic diversity(PD)in both gut and gill microbiomes,while Lactobacillus was significantly more abundant and showed strong network centrality in the gut of this group.Furthermore,beta diversity analysis revealed significant differences in microbial community structure between the groups in both gut and gills.Functional predictions indicated increased activity in metabolic pathways(e.g.,carbon metabolism,protein export)in the fast-growing group,reflecting the adaptations to increased energy utilization.Conversely,other genera such as TM7a were found at significantly lower abundance in the fast-growing group and showed sig-nificant correlations with growth parameters,highlighting potential microbial indicators of different growth strategies or physiological trade-offs.These findings underscore the close relationship between microbial communities and the health and productivity of Penaeus vannamei.Future studies should focus on optimizing growth perfor-mance and balancing it with the immune robustness in P.vannamei.展开更多
The object of this study is to investigate the effects of sodium alginate(SA)and CaCl_(2) on the physicochemical characteristics of shrimp surimi by studying gel strength,water holding capacity(WHC),as well as by text...The object of this study is to investigate the effects of sodium alginate(SA)and CaCl_(2) on the physicochemical characteristics of shrimp surimi by studying gel strength,water holding capacity(WHC),as well as by texture profile analysis(TPA).In addition,the mechanism was analyzed through chemical interactions,protein secondary structure,and microstructure.The results showed that with the addition of different concentrations of SA and CaCl_(2) to the shrimp surimi,the gel quality firstly increased and then decreased with the increase of SA and CaCl_(2) concentrations.The highest values of WHC,breaking force and gel strength were obtained with the addition of 1.2%SA or 0.1%CaCl_(2).When SA and CaCl_(2) were used in concert,the group containing 1.2%SA and 0.15%CaCl_(2) had the highest gel strength with the densest three-dimensional network structure of the gel.In addition,the results of chemical interaction analyses showed that hydrogen and ionic bonds were the main chemical bonds of shrimp surimi sol,while shrimp surimi gel mainly consisted of hydrophobic and disulfide bonds.The incorporation of SA and CaCl_(2) resulted in a significant increase in hydrophobic interactions and disulfide bonding,which could effectively improve the gel properties of shrimp surimi.展开更多
Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus vannamei.However,high temperature and pressure du...Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus vannamei.However,high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their inactivation.Further investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live Ep-M17.Therefore,we evaluated the effects of inactivated L.plantarum Ep-M17 on growth performance,immune response,disease resistance,and gut microbiota in P.vannamei.Results show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in shrimp.Also,histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abundance of B and R cells in the hepatopancreas.Additionally,the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and intestines.Moreover,it stimulated the expression of Lvlec,PEN-3a,Crustin,LGBP,Lysozyme,and proPo genes in both the hepatopancreas and intestines.Furthermore,the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora,facilitating the host organism’s metabolism and immunity to improve the disease resistance of shrimp.Therefore,supplementation of inactivated L.plantarum Ep-M17 in shrimp diets can exert similar effects as live L.plantarum Ep-M17 effectively improving growth performance,gut microbiota,immune response,and disease resistance in P.vannamei.展开更多
Shrimp(Penaeus vannamei)proteins have been shown an allergenic potential;however,little information is available on the sensitizing and eliciting capacity of shrimp protein digestion products.In this study,a BALB/c mi...Shrimp(Penaeus vannamei)proteins have been shown an allergenic potential;however,little information is available on the sensitizing and eliciting capacity of shrimp protein digestion products.In this study,a BALB/c mice model was used to explore the allergenicity of shrimp protein sample(SPS)and their gastric and gastrointestinal digestion products(GDS/GIDS).As compared with the SPS groups,the GDS/GIDS groups caused lower specific immunoglobulins(Ig E/Ig G1)levels(P<0.05),but higher than the control groups,indicating that the digestion products sensitized the mice.Meanwhile,spleen index,mouse mast cell protease-1(m MCP-1)concentration and proportion of degranulated mast cells were significantly reduced in the GDS/GIDS groups(P<0.05);simultaneously,allergic symptoms,vascular permeability and histopathological changes of tissues were alleviated.Nevertheless,the allergenicity of digestion products cannot be eliminated and still cause systemic allergic reactions in mice.The study showed that the digestion products of shrimp still had high sensitizing and eliciting capacity.展开更多
Genetic diversity of 32 individuals of P. chinensis in the Chinese coastal waters of the Huanghai and Bobal Seas was detected by RAPD technique. Twenty decamer primers of OPI were used for DNA amplification for each i...Genetic diversity of 32 individuals of P. chinensis in the Chinese coastal waters of the Huanghai and Bobal Seas was detected by RAPD technique. Twenty decamer primers of OPI were used for DNA amplification for each individual. The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty--nine of 106 loci detected were polymorphic, amounting to 36. 8 %. Mean genetic distance was 0. 094 1 0. 020 6; 68 markers (63. 2 % of the total) showed stable homogeneity in all of the 32 individuals.展开更多
The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus (Fenneropaeneus) chinensis was captured from west coast of the ...The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus (Fenneropaeneus) chinensis was captured from west coast of the Korean peninsula and mated with a "Yellow Sea No. 1" male to produce the first filial generation (F1) 100 F2 full-sib progeny from brother-sister crosses between F1 families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers, and 12 randomly amplified polymorphism (RAPD) markers. Forty-seven linkage groups (LGs) were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci (QTLs) in Chinese shrimp.展开更多
The genetic structure of three stocks (K, H and S) of Penaeus chinensis was detected by RAPD method which useed 41 random oligonucleotide primers with 10 bases. Stock K was from the spawning stock of the western coast...The genetic structure of three stocks (K, H and S) of Penaeus chinensis was detected by RAPD method which useed 41 random oligonucleotide primers with 10 bases. Stock K was from the spawning stock of the western coastal waters of the Korea Peninsula, H was from the Huanghai Sea-Bohai Sea stock and S was from the health cultured stock in Jimo, Shandong Province, China. Three genetic markers, F-02(540), OPN-13(800) and OPN-20(2200), which could differentiate the three different stocks, have been reported. The basic data for marker assisted selection (MAS) on genetic breeding of Penaeus chinensis are provided.展开更多
A kind of baculovirus was isolated from the cephalothorax homogenate of sick or morbid Penaeus chinensis by differential centrifugation and density gradient centrifugation. Electron microscopic examination of ultrathi...A kind of baculovirus was isolated from the cephalothorax homogenate of sick or morbid Penaeus chinensis by differential centrifugation and density gradient centrifugation. Electron microscopic examination of ultrathin section of the gills, stomach and mid-gut tissues also revealed the presence of rod-shaped baculoviral particles with the same size in the affected cell nuclei, where most of the virions arranging in cluster assembled and caused a series of cytopathic changes. The virion covered with bilaminal envelope was 320 ~ 400 nm × 100 ~ 130 nm in size, whereas the nucleocapsid ranged in size of 250~ 300 nm in length and 70 ~ 100 nm in breadth respectively. No nuclear polyhedron or granulin occlusion theies have been found in cells. According to the principle of viral classification, this newly found virus could probably belong to the non-occluded subgroup of insect baculoviridae, i. e., C subgroup baculovirus.展开更多
This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O’sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the auth...This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O’sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, andhepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of theepidemic in the northern regions in 1993.展开更多
The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of hos...The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal importance for improving its farming performance.In this study,the RNA-Seq platform was utilized to investigate transcriptomic changes of the hepatopancreas in pond-cultured P.monodon with HPND symptoms.A total of 62071 genes with an average length of 953 bp were obtained,and a lot of simple sequence repeat(SSR)loci related to these genes were identified.Totally,5204 differentially expressed genes(DEGs)were detected between healthy shrimp and HPND shrimp,with 3399 genes upregulated and 1,805 genes down-regulated.These genes had a wide range of biological functions,and several well-known immunerelated genes including integrin alpha 5,integrin beta 1,C type lectin and catalase were among the DEGs.Cell signaling pathways including the extracellular matrix(ECM)receptor interaction,phagosome and lysosome pathways were significantly upregulated in HPND-infected shrimps,indicating their involvement in the immune responses of shrimp against HPND.The data obtained in this study offers new insights into the molecular mechanisms of shrimp host response to HPND disease,and provides a resource for molecular marker development and genetic breeding studies of P.monodon.展开更多
The effects of rare earths (RE) and its complex with extract from seaweed (Lamiaria japonica) on the growth and immunization of penaeid shrimp (Penaeus vannamei) were investigated. The results show that the survival r...The effects of rare earths (RE) and its complex with extract from seaweed (Lamiaria japonica) on the growth and immunization of penaeid shrimp (Penaeus vannamei) were investigated. The results show that the survival rate, body length and weight of the shrimp treated by RE and its complex with extract from seaweed are higher than those of the control, and that the activities of the phenoloxidase (PO), lysozyme (LSZ), superoxide dismutase (SOD), peroxidase (POD), acid phosphatase (ACP) and alkaline phosphatase (ALP) in the shrimp treated by RE and the complex are also much higher than those from the control.展开更多
Genetic diversity of two wild Penaeus monodon populations sampled from the coastal waters of Qinglan(Hainan Province of China,HN)and Malaysia(KD),and the F1 generation of a Thailand broodstock population(CP)were exami...Genetic diversity of two wild Penaeus monodon populations sampled from the coastal waters of Qinglan(Hainan Province of China,HN)and Malaysia(KD),and the F1 generation of a Thailand broodstock population(CP)were examined by vertical polyacrylamide gel electrophoresis.Of 21 loci encoded by ten enzymes,11 were polymorphic.The mean proportions of polymorphic loci of HN,KD and CP were 36.36%,45.45%and 50.00%,with the average heterozygosities of 0.135,0.181 and 0.191,and the effective numbers of alleles per loci were 1.300,1.330 and 1.329,respectively.The divergent indexes of HN,KD and CP were0.023,0.124,and 0.117,respectively.The genetic distance between the two wild populations was 0.005,and the gene differentiation coefficient was also very low(0.014).The results indicated that the F1 generation population had a higher genetic diversity than the two wild populations,and that the HN population had the lowest one.There was no significant differentiation between HN and KD populations and all populations existed with a slight heterozygote excess.展开更多
Although Vibrio parahaemolyticus has become the most common pathogen in fresh and frozen seafood,its virulence changes have often been ignored during the processing of seafood.To investigate these potential risks,we u...Although Vibrio parahaemolyticus has become the most common pathogen in fresh and frozen seafood,its virulence changes have often been ignored during the processing of seafood.To investigate these potential risks,we used frozen Penaeus chinensis as examples,and the most virulent factors of V.parahaemolyticus,including amounts,viable but nonculturable(VBNC)status,toxins TDH and TRH,and virulence genes tdh and trh,were determined.Bacterial quantities were signifi cantly reduced during drain and sprinkling phases,but caused by different factors.By SYTO9 and PI staining showed that washing was the main reason for the bacterial reduction at the drain phase,while the strain entering VBNC state was another reason at sprinkling phase.Their hemolysis toxicity,produced by TDH and TRH,became stronger after inoculation on shrimp,and could be detected throughout the process.Moreover,tdh and trh also exhibited trends similar to that of the hemolysis toxicity test.tdh was almost to a two-fold expression level during ice-glazing phase,while trh only express at a low level,less than half of the expression level before inoculation.These results demonstrated that the strains were not dead during freezing process,but became VBNC cells,which still produced and accumulated toxins,especially TDH,the most virulent factor.展开更多
Heat shocks effectively produced triploids in Penaeus chinensis . Fertilized eggs heat shocked (28-32℃) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy induction rates of 39%-...Heat shocks effectively produced triploids in Penaeus chinensis . Fertilized eggs heat shocked (28-32℃) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy induction rates of 39%-75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male shrimp, there were no such differences.展开更多
White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles, EcoR I digested fragments of the WSSV genome were cloned; three of thes...White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles, EcoR I digested fragments of the WSSV genome were cloned; three of these fragments were used as non radioactive probes labeled with DIG 11 dUTP. The probes hybridized in situ, with sections located in the nuclei of all WSSV infected tissues. The virus was detected in the gill, stomach, epidermis, and connective tissue and so on, but not detected in healthy shrimp tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.展开更多
基金Supported by the Natural Science Foundation of Xiamen (No. 3502Z20227322)the Natural Science Key Foundation of Fujian Province (No. 2020J02002)+1 种基金the Social Science Foundation of Fujian Province (No. FJ2024BF047)the Financial Research Projects of Fujian Province (No. 2024-48)。
文摘Penaeus vannamei, known as the whiteleg shrimp, holds significant economic importance in aquaculture. The intensive culture of P. vannamei poses substantial environmental risks, particularly in fragile ecosystems like estuarine mangroves. Although there is a consensus on the pollution and harmful effects of microplastics(MPs), study on the pollution and potential risks posed by P. vannamei in estuary mangroves remains scarce. Therefore, the extent of microplastic pollution was evaluated and the correlation between the properties of MPs and the potential risks they pose to P. vannamei was examined.The average MP abundance in P. vannamei from Jiulong River estuary was determined to be 0.46±0.03 n/g in five different polymers, i.e., polypropylene(PP), polyethylene(PE), polystyrene(PS), polyethylene terephthalate(PET), and polyvinyl chloride resin(PVC). The total percentage of PS, PE, and PET polymers was the greatest(73.85%), where PET and PP(26.16%) were highly correlated in size and shape. Small MPs(1-200 μm) come in irregular particles, fragments, films, and large-sized MPs(200-1 000 μm) were mainly foam-shaped. The hazard risk level of MPs in P. vannamei from Jiulong River estuary reached grade Ⅲ, and the overall hazard risk index(H) value was 593.66. Among all samples,PVC polymer accounted for the lowest proportion(5.52%), but the H value was as high as 582.42, which contributed 98.11% to the overall hazard risk index. Regardless of global or Chinese, the microplastic pollution of P. vannamei in the estuary is at a moderate level. The protocol for MPs characteristics and correlation in seafood should be the basis for the risk assessment framework. Further studies are needed to evaluate how the exposure to MPs poses a risk for human health.
基金funded by the Science and Technology Project of Zhanjiang(Nos.2019A03011,2022A01040)the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(No.SML2023SP234).
文摘The relationship between microbial communities and their hosts is important to the health and productivity of marine ani-mals in aquaculture.This study investigated the microbiomes in the gut and gill of Penaeus vannamei using 16S rRNA gene sequencing,comparing the microbial characteristics in the fast-growing trait and in the control group.Fast-growing individuals exhibited sig-nificantly different growth parameters and gut/gill microbiomes.Significantly higher weight gain and condition factors,as well as lower hepatosomatic indices(HSI),were observed in fast-growing individuals.Fast-growing individuals also displayed significantly higher operational taxonomic unit(OTU)counts(in gills)and greater phylogenetic diversity(PD)in both gut and gill microbiomes,while Lactobacillus was significantly more abundant and showed strong network centrality in the gut of this group.Furthermore,beta diversity analysis revealed significant differences in microbial community structure between the groups in both gut and gills.Functional predictions indicated increased activity in metabolic pathways(e.g.,carbon metabolism,protein export)in the fast-growing group,reflecting the adaptations to increased energy utilization.Conversely,other genera such as TM7a were found at significantly lower abundance in the fast-growing group and showed sig-nificant correlations with growth parameters,highlighting potential microbial indicators of different growth strategies or physiological trade-offs.These findings underscore the close relationship between microbial communities and the health and productivity of Penaeus vannamei.Future studies should focus on optimizing growth perfor-mance and balancing it with the immune robustness in P.vannamei.
基金supported by the Public Welfare Project of Ningbo(No.2022S211)the National Key R&D Program of China(No.2020YFD0900903)。
文摘The object of this study is to investigate the effects of sodium alginate(SA)and CaCl_(2) on the physicochemical characteristics of shrimp surimi by studying gel strength,water holding capacity(WHC),as well as by texture profile analysis(TPA).In addition,the mechanism was analyzed through chemical interactions,protein secondary structure,and microstructure.The results showed that with the addition of different concentrations of SA and CaCl_(2) to the shrimp surimi,the gel quality firstly increased and then decreased with the increase of SA and CaCl_(2) concentrations.The highest values of WHC,breaking force and gel strength were obtained with the addition of 1.2%SA or 0.1%CaCl_(2).When SA and CaCl_(2) were used in concert,the group containing 1.2%SA and 0.15%CaCl_(2) had the highest gel strength with the densest three-dimensional network structure of the gel.In addition,the results of chemical interaction analyses showed that hydrogen and ionic bonds were the main chemical bonds of shrimp surimi sol,while shrimp surimi gel mainly consisted of hydrophobic and disulfide bonds.The incorporation of SA and CaCl_(2) resulted in a significant increase in hydrophobic interactions and disulfide bonding,which could effectively improve the gel properties of shrimp surimi.
基金Supported by the Zhejiang Provincial Natural Science Foundation of China(No.LY23D060002)the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(No.2021C02069-5)+1 种基金the Pingyang County Science and Technology Strengthening Agriculture Industry Upgrading Project(No.2023PY003)the National Natural Science Foundation of China(No.41906107)。
文摘Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus vannamei.However,high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their inactivation.Further investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live Ep-M17.Therefore,we evaluated the effects of inactivated L.plantarum Ep-M17 on growth performance,immune response,disease resistance,and gut microbiota in P.vannamei.Results show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in shrimp.Also,histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abundance of B and R cells in the hepatopancreas.Additionally,the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and intestines.Moreover,it stimulated the expression of Lvlec,PEN-3a,Crustin,LGBP,Lysozyme,and proPo genes in both the hepatopancreas and intestines.Furthermore,the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora,facilitating the host organism’s metabolism and immunity to improve the disease resistance of shrimp.Therefore,supplementation of inactivated L.plantarum Ep-M17 in shrimp diets can exert similar effects as live L.plantarum Ep-M17 effectively improving growth performance,gut microbiota,immune response,and disease resistance in P.vannamei.
基金financially supported by the National Natural Science Foundation of China(32022067)the Dalian Sci-Tech Talent Innovation Support Program(2022RY04)。
文摘Shrimp(Penaeus vannamei)proteins have been shown an allergenic potential;however,little information is available on the sensitizing and eliciting capacity of shrimp protein digestion products.In this study,a BALB/c mice model was used to explore the allergenicity of shrimp protein sample(SPS)and their gastric and gastrointestinal digestion products(GDS/GIDS).As compared with the SPS groups,the GDS/GIDS groups caused lower specific immunoglobulins(Ig E/Ig G1)levels(P<0.05),but higher than the control groups,indicating that the digestion products sensitized the mice.Meanwhile,spleen index,mouse mast cell protease-1(m MCP-1)concentration and proportion of degranulated mast cells were significantly reduced in the GDS/GIDS groups(P<0.05);simultaneously,allergic symptoms,vascular permeability and histopathological changes of tissues were alleviated.Nevertheless,the allergenicity of digestion products cannot be eliminated and still cause systemic allergic reactions in mice.The study showed that the digestion products of shrimp still had high sensitizing and eliciting capacity.
基金the National Natural Science Foundation of China finder the Project! No. 39620260.
文摘Genetic diversity of 32 individuals of P. chinensis in the Chinese coastal waters of the Huanghai and Bobal Seas was detected by RAPD technique. Twenty decamer primers of OPI were used for DNA amplification for each individual. The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty--nine of 106 loci detected were polymorphic, amounting to 36. 8 %. Mean genetic distance was 0. 094 1 0. 020 6; 68 markers (63. 2 % of the total) showed stable homogeneity in all of the 32 individuals.
基金Supported by National Key Technology R&D Program(No.2006BAD01A13)National Special Research Fund for Nonprofit Sector(Agriculture)(No.nyhyzx07-042)the Earmarked Fund for Modern Agro-industry Technology Research System(No.nycytx-46)
文摘The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus (Fenneropaeneus) chinensis was captured from west coast of the Korean peninsula and mated with a "Yellow Sea No. 1" male to produce the first filial generation (F1) 100 F2 full-sib progeny from brother-sister crosses between F1 families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers, and 12 randomly amplified polymorphism (RAPD) markers. Forty-seven linkage groups (LGs) were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci (QTLs) in Chinese shrimp.
文摘The genetic structure of three stocks (K, H and S) of Penaeus chinensis was detected by RAPD method which useed 41 random oligonucleotide primers with 10 bases. Stock K was from the spawning stock of the western coastal waters of the Korea Peninsula, H was from the Huanghai Sea-Bohai Sea stock and S was from the health cultured stock in Jimo, Shandong Province, China. Three genetic markers, F-02(540), OPN-13(800) and OPN-20(2200), which could differentiate the three different stocks, have been reported. The basic data for marker assisted selection (MAS) on genetic breeding of Penaeus chinensis are provided.
文摘A kind of baculovirus was isolated from the cephalothorax homogenate of sick or morbid Penaeus chinensis by differential centrifugation and density gradient centrifugation. Electron microscopic examination of ultrathin section of the gills, stomach and mid-gut tissues also revealed the presence of rod-shaped baculoviral particles with the same size in the affected cell nuclei, where most of the virions arranging in cluster assembled and caused a series of cytopathic changes. The virion covered with bilaminal envelope was 320 ~ 400 nm × 100 ~ 130 nm in size, whereas the nucleocapsid ranged in size of 250~ 300 nm in length and 70 ~ 100 nm in breadth respectively. No nuclear polyhedron or granulin occlusion theies have been found in cells. According to the principle of viral classification, this newly found virus could probably belong to the non-occluded subgroup of insect baculoviridae, i. e., C subgroup baculovirus.
文摘This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O’sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, andhepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of theepidemic in the northern regions in 1993.
基金This study was funded in part by the Natural Science Foundation of Guangdong Province(No.2018A030310049)2019 Annual Guangdong Provincial Special fund of Nan-hai Economic Shrimp Breeding and Culture Laboratory(No.2319412525)+2 种基金the Nanhai Scholar Project of Guangdong Ocean University,Fangchenggang Science and Technol-ogy Plan Project(No.AD19008017)the R&D Program of Key Areas in Guangdong Province(No.2020B0202010009)2019 Provincial Financial Special Fund Con-struction Project of Guangdong Ocean University(No.231619003).
文摘The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal importance for improving its farming performance.In this study,the RNA-Seq platform was utilized to investigate transcriptomic changes of the hepatopancreas in pond-cultured P.monodon with HPND symptoms.A total of 62071 genes with an average length of 953 bp were obtained,and a lot of simple sequence repeat(SSR)loci related to these genes were identified.Totally,5204 differentially expressed genes(DEGs)were detected between healthy shrimp and HPND shrimp,with 3399 genes upregulated and 1,805 genes down-regulated.These genes had a wide range of biological functions,and several well-known immunerelated genes including integrin alpha 5,integrin beta 1,C type lectin and catalase were among the DEGs.Cell signaling pathways including the extracellular matrix(ECM)receptor interaction,phagosome and lysosome pathways were significantly upregulated in HPND-infected shrimps,indicating their involvement in the immune responses of shrimp against HPND.The data obtained in this study offers new insights into the molecular mechanisms of shrimp host response to HPND disease,and provides a resource for molecular marker development and genetic breeding studies of P.monodon.
基金Project supported by Qingdao Scientific Research Program (03 2 HH 3) and Shandong Scientific Foundation (2004ZRB01001)
文摘The effects of rare earths (RE) and its complex with extract from seaweed (Lamiaria japonica) on the growth and immunization of penaeid shrimp (Penaeus vannamei) were investigated. The results show that the survival rate, body length and weight of the shrimp treated by RE and its complex with extract from seaweed are higher than those of the control, and that the activities of the phenoloxidase (PO), lysozyme (LSZ), superoxide dismutase (SOD), peroxidase (POD), acid phosphatase (ACP) and alkaline phosphatase (ALP) in the shrimp treated by RE and the complex are also much higher than those from the control.
基金The National Natural Science Foundation of China under contract No.30471322.
文摘Genetic diversity of two wild Penaeus monodon populations sampled from the coastal waters of Qinglan(Hainan Province of China,HN)and Malaysia(KD),and the F1 generation of a Thailand broodstock population(CP)were examined by vertical polyacrylamide gel electrophoresis.Of 21 loci encoded by ten enzymes,11 were polymorphic.The mean proportions of polymorphic loci of HN,KD and CP were 36.36%,45.45%and 50.00%,with the average heterozygosities of 0.135,0.181 and 0.191,and the effective numbers of alleles per loci were 1.300,1.330 and 1.329,respectively.The divergent indexes of HN,KD and CP were0.023,0.124,and 0.117,respectively.The genetic distance between the two wild populations was 0.005,and the gene differentiation coefficient was also very low(0.014).The results indicated that the F1 generation population had a higher genetic diversity than the two wild populations,and that the HN population had the lowest one.There was no significant differentiation between HN and KD populations and all populations existed with a slight heterozygote excess.
基金funded by grants from the National Key Research and Development Program of China[2018YFC1602201]Key Research and Development Program of Shaanxi Province[2021NY-158]+1 种基金the National Natural Science Foundation of China[31671780]the Agricultural Product Quality and Safety Risk Assessment Foundation of the Ministry of Agriculture and Rural Affairs of the People’s Republic of China[GJFP2020002,GJFP2020003].
文摘Although Vibrio parahaemolyticus has become the most common pathogen in fresh and frozen seafood,its virulence changes have often been ignored during the processing of seafood.To investigate these potential risks,we used frozen Penaeus chinensis as examples,and the most virulent factors of V.parahaemolyticus,including amounts,viable but nonculturable(VBNC)status,toxins TDH and TRH,and virulence genes tdh and trh,were determined.Bacterial quantities were signifi cantly reduced during drain and sprinkling phases,but caused by different factors.By SYTO9 and PI staining showed that washing was the main reason for the bacterial reduction at the drain phase,while the strain entering VBNC state was another reason at sprinkling phase.Their hemolysis toxicity,produced by TDH and TRH,became stronger after inoculation on shrimp,and could be detected throughout the process.Moreover,tdh and trh also exhibited trends similar to that of the hemolysis toxicity test.tdh was almost to a two-fold expression level during ice-glazing phase,while trh only express at a low level,less than half of the expression level before inoculation.These results demonstrated that the strains were not dead during freezing process,but became VBNC cells,which still produced and accumulated toxins,especially TDH,the most virulent factor.
文摘Heat shocks effectively produced triploids in Penaeus chinensis . Fertilized eggs heat shocked (28-32℃) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy induction rates of 39%-75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male shrimp, there were no such differences.
文摘White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles, EcoR I digested fragments of the WSSV genome were cloned; three of these fragments were used as non radioactive probes labeled with DIG 11 dUTP. The probes hybridized in situ, with sections located in the nuclei of all WSSV infected tissues. The virus was detected in the gill, stomach, epidermis, and connective tissue and so on, but not detected in healthy shrimp tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.
