Changes in the pattern of organization of microtubules in the meiotic stages of development of pollen (i.e. from pre-meiotic interphase to more or less metaphase I) of a normal (IR36) and a temperature/photoperiod sen...Changes in the pattern of organization of microtubules in the meiotic stages of development of pollen (i.e. from pre-meiotic interphase to more or less metaphase I) of a normal (IR36) and a temperature/photoperiod sensitive male sterile line (Peiai 64S) of rice were studied using immunofluorescence confocal microscopy. In IR36, from pre-meiotic interphase to metaphase I, the pattern of microtubule distribution in the meiocytes underwent a series of changes. Some new organizational patterns of microtubules (that have not been described before) were observed during microsporogenesis, including the existence of a broad band of perinuclear microtubules at the diakinesis stage of development. The pattern of microtubule distribution in the meiocytes of the male sterile line, Peiai 64S, was quite different front that seen in IR36. In Peiai 64S, the microtubules showed abnormal patterns of distribution from pre-meiotic interphase to metaphase I. For example the broad band of perinuclear microtubules seen at diakinesis in IR36 was much disorganized and loosened in Peiai 64S. The spindles formed were also very abnormal and different from the normal spindle. The appearance of abnormal microtubule distribution in the early stages of microsporogenesis may contribute to the malformation and ultimate abortion of pollen in Peiai 64S.展开更多
Peiai64S, an indica male sterile rice with a male fertility alteration under different environments, is selected from the offspring of indica rice crossed with Nongken58S. Nongken58S, a japonica pho-toperiod sensitive...Peiai64S, an indica male sterile rice with a male fertility alteration under different environments, is selected from the offspring of indica rice crossed with Nongken58S. Nongken58S, a japonica pho-toperiod sensitive genie male sterile rice (PGMS), deriving from a natural mutant plant individual of normal japonica rice variety, Nongken58, is used as a male sterile gene donor of Peiai64S. But Peiai64S is not a typical PGMS rice, the male fertility is sensitive to temperature just as thermo-sensitive genie male sterile rice (TGMS). We have selected typical PGMS plants in F2 population of Peiai64S× Nongken58, whose ratio of fertile plants to sterile plants is nearly 3:1. The sterility inheritance conformed to one pair of gene segregation model. The result indicates the main male sterile gene in Peiai64S is not other than the PGMS gene, and comes from Nongken58S. The genetic background affects effective expression of the PGMS gene. This suggests that we ought to focus on optimizing the genetic background of the PGMS gene in PGMS rice breeding, and select an ideal genetic background as a transgenic background in molecular breeding.展开更多
[Objective] The purpose was to study and analyze the difference of Peiai 64 S fertility expression in two different climatic regions, Hainan and Hunan in 2010, and confirm the suitable region for its production of hyb...[Objective] The purpose was to study and analyze the difference of Peiai 64 S fertility expression in two different climatic regions, Hainan and Hunan in 2010, and confirm the suitable region for its production of hybrid seeds. [Method] Eight sowing times were designed, March 20 and 30, April 10 and 30, May 15, June 1, 10 and 20, respectively in Changsha of Hunan. Thirteen sowing times were designed in Hainan, January 28, February 12 and 27, March 14 and 29, April 13 and 28, May 13 and 28, June 12, July 1, 12 and 27, respectively. Peiai 64S was gradually managed and recorded heading stage during cultivation, and the effects of different climate influencing factors, such as daily mean temperature, daily maximum temperature, daily minimum temperature, duration of day at 0-25 d before heading （namely pollen mother cell meiophase）, on pollen fertility were analyzed to confirm sensitive periods of light and temperature. [ResuLt] There was a big difference in sensitive period among the same cytoplasmic male sterile （CMS） in different climatic regions. The sensitive period of Peiai 64S was in first four phases, phases V, VI, VII, VIII （0-13 d） in Changsha of Hunan, which was in last four phases, phases I, II, III, IV （13-25 d） in Haikou of Hainan. Under natural conditions, Peiai 64S pollen sterility rate were all more than 99.5% and seed-setting rates all less than 0.5% in Haikou, so production of hybrid seeds was safe. Its pollen sterility rate was 21.3%- 100.0% in Hunan, so its security coefficient of hybrid seeds production was lower than that in Hainan. [Conclusion] The security coefficient of hybrid seeds production of CMS Peiai 64S is lower than that in Hainan.展开更多
文摘Changes in the pattern of organization of microtubules in the meiotic stages of development of pollen (i.e. from pre-meiotic interphase to more or less metaphase I) of a normal (IR36) and a temperature/photoperiod sensitive male sterile line (Peiai 64S) of rice were studied using immunofluorescence confocal microscopy. In IR36, from pre-meiotic interphase to metaphase I, the pattern of microtubule distribution in the meiocytes underwent a series of changes. Some new organizational patterns of microtubules (that have not been described before) were observed during microsporogenesis, including the existence of a broad band of perinuclear microtubules at the diakinesis stage of development. The pattern of microtubule distribution in the meiocytes of the male sterile line, Peiai 64S, was quite different front that seen in IR36. In Peiai 64S, the microtubules showed abnormal patterns of distribution from pre-meiotic interphase to metaphase I. For example the broad band of perinuclear microtubules seen at diakinesis in IR36 was much disorganized and loosened in Peiai 64S. The spindles formed were also very abnormal and different from the normal spindle. The appearance of abnormal microtubule distribution in the early stages of microsporogenesis may contribute to the malformation and ultimate abortion of pollen in Peiai 64S.
基金supported by the 9th“Five-Year-Plan”key technology projects in the Bio tech field of“863”program.
文摘Peiai64S, an indica male sterile rice with a male fertility alteration under different environments, is selected from the offspring of indica rice crossed with Nongken58S. Nongken58S, a japonica pho-toperiod sensitive genie male sterile rice (PGMS), deriving from a natural mutant plant individual of normal japonica rice variety, Nongken58, is used as a male sterile gene donor of Peiai64S. But Peiai64S is not a typical PGMS rice, the male fertility is sensitive to temperature just as thermo-sensitive genie male sterile rice (TGMS). We have selected typical PGMS plants in F2 population of Peiai64S× Nongken58, whose ratio of fertile plants to sterile plants is nearly 3:1. The sterility inheritance conformed to one pair of gene segregation model. The result indicates the main male sterile gene in Peiai64S is not other than the PGMS gene, and comes from Nongken58S. The genetic background affects effective expression of the PGMS gene. This suggests that we ought to focus on optimizing the genetic background of the PGMS gene in PGMS rice breeding, and select an ideal genetic background as a transgenic background in molecular breeding.
文摘[Objective] The purpose was to study and analyze the difference of Peiai 64 S fertility expression in two different climatic regions, Hainan and Hunan in 2010, and confirm the suitable region for its production of hybrid seeds. [Method] Eight sowing times were designed, March 20 and 30, April 10 and 30, May 15, June 1, 10 and 20, respectively in Changsha of Hunan. Thirteen sowing times were designed in Hainan, January 28, February 12 and 27, March 14 and 29, April 13 and 28, May 13 and 28, June 12, July 1, 12 and 27, respectively. Peiai 64S was gradually managed and recorded heading stage during cultivation, and the effects of different climate influencing factors, such as daily mean temperature, daily maximum temperature, daily minimum temperature, duration of day at 0-25 d before heading （namely pollen mother cell meiophase）, on pollen fertility were analyzed to confirm sensitive periods of light and temperature. [ResuLt] There was a big difference in sensitive period among the same cytoplasmic male sterile （CMS） in different climatic regions. The sensitive period of Peiai 64S was in first four phases, phases V, VI, VII, VIII （0-13 d） in Changsha of Hunan, which was in last four phases, phases I, II, III, IV （13-25 d） in Haikou of Hainan. Under natural conditions, Peiai 64S pollen sterility rate were all more than 99.5% and seed-setting rates all less than 0.5% in Haikou, so production of hybrid seeds was safe. Its pollen sterility rate was 21.3%- 100.0% in Hunan, so its security coefficient of hybrid seeds production was lower than that in Hainan. [Conclusion] The security coefficient of hybrid seeds production of CMS Peiai 64S is lower than that in Hainan.
