The aim of this study was to isolate algicidal bacteria so as to control harmful cyanobacterium Microcystis aeruginosa （M. aeruginosa） blooms using biological methods. Nine bacterial strains were isolated to inhibit...The aim of this study was to isolate algicidal bacteria so as to control harmful cyanobacterium Microcystis aeruginosa （M. aeruginosa） blooms using biological methods. Nine bacterial strains were isolated to inhibit the growth ofM. aeruginosa, among which the Mail 1-5 bacterial strain exhibited remarkable algicidal activity against M. aeruginosa cells during the test. Based on the 16S rDNA analysis, the isolated Mail 1-5 was identified as Pedobacter sp. through morphology and homology research. The results of cocultivation of the cyanobacteria with MaI 11-5 algicidal isolates showed obvious algicidal activity against cyanobacterial cells. The algicidal effect of Mail 1-5 exceeded 50% after two days, exceeded 70% after four days, and reached 80% after seven days. The observation results with a scanning electron microscope showed that the cyanobacterial cells aggregated and produced mucous-like substances when cocultivated with the algicidal bacteria. The results indicated that the Mail 1-5 bacterial strain may possess a novel function for controlling harmful blooms and further studies will provide new insights into its role in water environment.展开更多
基金supported by the Basic Research Program of Jiangsu Province(Grant No.BK2012828)the grant of Greater Nagoya Project in Environmental Sciencethe Open Laboratory Project of Nanjing University of Technology(Grant No.2012-2013-138)
文摘The aim of this study was to isolate algicidal bacteria so as to control harmful cyanobacterium Microcystis aeruginosa （M. aeruginosa） blooms using biological methods. Nine bacterial strains were isolated to inhibit the growth ofM. aeruginosa, among which the Mail 1-5 bacterial strain exhibited remarkable algicidal activity against M. aeruginosa cells during the test. Based on the 16S rDNA analysis, the isolated Mail 1-5 was identified as Pedobacter sp. through morphology and homology research. The results of cocultivation of the cyanobacteria with MaI 11-5 algicidal isolates showed obvious algicidal activity against cyanobacterial cells. The algicidal effect of Mail 1-5 exceeded 50% after two days, exceeded 70% after four days, and reached 80% after seven days. The observation results with a scanning electron microscope showed that the cyanobacterial cells aggregated and produced mucous-like substances when cocultivated with the algicidal bacteria. The results indicated that the Mail 1-5 bacterial strain may possess a novel function for controlling harmful blooms and further studies will provide new insights into its role in water environment.
