This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici str...This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici strains with antibacterial activity(P.acidilactici CCFM28 and CCFM18)were obtained based on the inhibition-zone assay.The produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide treatment.Bacteriocin-producing and non-bacteriocin-producing P.acidilactici strains(P.acidilactici CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal mice.After 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum level.At the genus level,the administration of three P.acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus.Furthermore,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of strain.The results indicated that the intervention of different P.acidilactici strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.展开更多
In this study,α-amylase andα-glucosidase inhibition activities of cell-free supernatant(CFS)of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions.Si...In this study,α-amylase andα-glucosidase inhibition activities of cell-free supernatant(CFS)of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions.Significant inhibition activities were detected in Pediococcus acidilactici FM-Pa-JXM94,L15,GC215 and L31,and their probiotic properties were further assessed.Following the inhibitory activities onα-glucosidase andα-amylase,the viability of simulated gastric juice and adhesive ability,FM-Pa-JXM94 was screened and identified as Pediococcus acidilactici by 16S rDNA nucleotide sequencing,and the potential hypoglycemic mechanism was explored using IR-HepG2.CFS,cell-free extract(CFE)and bacterial cells of FM-Pa-JXM94 demonstrated a strong potential to induce glucose consumption,glycogen synthesis,and pyruvate kinase activity,as well as decrease phosphoenolpyruvate carboxykinase activity of IR-HepG2 cells.Additionally,RT-qPCR results showed that CFS,CFE and bacteria cells upregulated the mRNA expression of IRS1,AKT,GYS2 and PI3K genes and downregulated the mRNA expression of GSK3β,FOXO1,PEPCK and G-6-Pase genes of IRS-1/PI3K/AKT/GSK3β/GYS2 and PI3K/AKT/FOXO1/PEPCK-G6Pase signal pathways.These findings illustrate that the different FM-Pa-JXM94 derivatives could alleviate insulin resistance of IR-HepG2 cells from key enzyme activity and gene levels.This study showcases FM-Pa-JXM94 as a promising probiotic candidate for preparing functional foods and hypoglycemic function supplements.展开更多
Maternal health plays a crucial role in fetal neurodevelopment,with high-fat diet(HFD)consumption before or during pregnancy known to induce neurodevelopmental and behavioral abnormalities in offspring.In this study,w...Maternal health plays a crucial role in fetal neurodevelopment,with high-fat diet(HFD)consumption before or during pregnancy known to induce neurodevelopmental and behavioral abnormalities in offspring.In this study,we assessed whether supplementation with Pediococcus acidilactici CCFM1344(10^(9) CFU/day,oral gavage)during pregnancy in C57BL/6J mice could serve as an effective strategy to counteract neurodevelopmental impairments in offspring induced by maternal HFD.Female mice(8 weeks old)were used for breeding,and both male and female offspring were included in the analyses.16S rRNA gene sequencing revealed that CCFM1344 significantly modulated the maternal gut microbiota composition,reduced the Firmicutes-to-Bacteroidetes(F/B)ratio,and enriched beneficial genera Alloprevotella and Rikenellaceae RC9 gut group.Behavioral assessments,including the open field and three-chamber social interaction tests,demonstrated that CCFM1344 effectively ameliorated hyperactivity and social deficits in juvenile offspring,with these behavioral changes showing partial normalization in adulthood.Gut microbiota analysis of the juvenile offspring indicated that CCFM1344 significantly affected microbial diversity and improved ecological stability.In mothers,fecal metabolomics and serum cytokine profiling showed that CCFM1344 intervention regulated key metabolites,including taurine and Lhistidine,and modulated inflammatory markers such as TNF-α,IFN-γ,and IL-10.In addition,transcriptomic and quantitative real-time PCR analyses of neonatal brain tissue revealed that CCFM1344 reversed the abnormal expression of genes associated with neuroinflammation and myelination—including Gfap,Mmp9,Il-1β,and Mobp—with these changes normalizing by adulthood.In conclusion,pregnancy-specific administration of CCFM1344 modulated the maternal gut microbiota and immunometabolic environment,alleviating HFDinduced impairments in early brain development and behavior in offspring.These findings highlight the critical importance of probiotic interventions during pregnancy in supporting neurodevelopmental health in the next generation.展开更多
The immune system plays a crucial role in maintaining host defense and homeostasis through complex cellular and molecular interactions.This study evaluated the immunostimulatory effects of the paraprobiotic Pediococcu...The immune system plays a crucial role in maintaining host defense and homeostasis through complex cellular and molecular interactions.This study evaluated the immunostimulatory effects of the paraprobiotic Pediococcus acidilactici SY23 in combination with chondroitin sulfate(CS)on RAW 264.7 murine macrophages.Co-treatment significantly enhanced cell viability and upregulated the production of nitric oxide(NO)and prostaglandin E2(PGE2)compared to individual treatments.In addition,phagocytic activity and intracellular reactive oxygen species(ROS)levels were markedly elevated.Co-treatment also induced substantial increases in the mRNA levels of pro-inflammatory cytokines,including interleukin(IL)-1β,IL-6,and tumor necrosis factor-α(TNF-α).The observed immunostimulatory responses were linked to the activation of nuclear factor-kappa B(NF-κB)and mitogen-activated protein kinase(MAPK)signaling cascades,as demonstrated by elevated phosphorylation levels of p65,ERK,JNK,and p38.Furthermore,the expression of inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)was markedly upregulated following co-treatment.These findings demonstrate that paraprobiotic P.acidilactici SY23,in combination with CS,promotes macrophage-mediated immune activation via the NF-κB and MAPK pathways.This suggests potential applicability of the formulation as a safe and effective immunomodulatory agent in the development of functional foods or nutraceuticals.展开更多
Pediococcus acidilactici has demonstrated promising potential as a psychobiotic with positive effects on gut health and depressive behaviors.Prior research has found that the relative levels of lactate in the intestin...Pediococcus acidilactici has demonstrated promising potential as a psychobiotic with positive effects on gut health and depressive behaviors.Prior research has found that the relative levels of lactate in the intestines of both depressed patients and mice are different from normal levels.In this study,we established a chronic unpredictable mild stress mouse model to compare the effects of high-lactate-producing P.acidilactici strain CCFM1344 and low-lactate-producing strain FSDLZ42M3 on depressive symptoms.Our experimental results showed that after four weeks of intervention,CCFM1344 significantly alleviated anxiety-like and depression-like behaviors induced by CUMS.Furthermore,CCFM1344 restored serum LPS levels,as well as the abnormally elevated LPS receptor protein and downstream pro-inflammatory cytokine genes expression in the hippocampus.Conversely,FSDLZ42M3 did not exhibit these effects.Notably,CCFM1344 was found to inhibit the abnormal activation of microglia for the first time.Additionally,16S rDNA V3-V4 sequencing and untargeted metabolomics assays revealed that CCFM1344 intervention improved gut microbiota dysbiosis.Specifically,it increased the relative abundance of the Lachnospiraceae NK4A136 group and regulated the aberrant intestinal metabolites in mice.These findings expand the potential applications of P.acidilactici as a probiotic and provide theoretical guidance for using CCFM1344 to prevent,alleviate,and treat neurological dysfunction.展开更多
Okara is produced in large quantities annually in China,but much of it is discarded due to its high content of indigestible dietary fiber(DF),contributing to significant environmental challenges.Recognizing the undere...Okara is produced in large quantities annually in China,but much of it is discarded due to its high content of indigestible dietary fiber(DF),contributing to significant environmental challenges.Recognizing the underexplored medicinal potential of DF,we developed an efficient fermentation method to enhance the bioavailability of okara fiber.In this study,Pediococcus acidilactici IFJ-1,which has strong enzymatic production capabilities and beneficial effects on gastrointestinal flora modulation,was selected to ferment okara.Results showed decreases in viscosity and particle size,optimized surface structure,improved thermal stability and hydration properties,and a significant increase in soluble DF content from 1.85%to 3.91%.To evaluate the physiological effects,hyperlipidemic mouse models were established and subjected to dietary interventions utilizing okara and fermented okara to measure changes in physicochemical parameters,gut microbiota composition,and lipid metabolism.The dietary intervention was effective,particularly in the fermented okara group,showing a 7.3%weight loss,improved blood lipids(triglycerides:‒39.8%,total cholesterol:‒12.8%,low-density lipoprotein cholesterol:‒34.2%,high-density lipoprotein cholesterol:+26.2%),and a 22.2%lower liver index.Gut microbiota analysis revealed that fermented okara positively modulated the microbial community by increasing the abundance of beneficial bacteria(e.g.,Bacteroidota)and reducing the abundance of obesity-associated bacteria(e.g.,Bacillota).Lipid metabolism profiling further demonstrated that fermented okara downregulated harmful lipids(e.g.,(O-acyl)-ω-hydroxy fatty acids,ceramides,and diacylglycerols)while upregulating beneficial phospholipids(e.g.,phosphatidylinositol,phosphatidylserine,phosphatidylethanolamine,lysophosphatidylinositol and lysophosphatidic acid).This study highlights a novel approach for enhancing DF utilization through fermentation,providing valuable insights into strategies for preventing obesity and metabolic diseases.展开更多
Biodetoxification fungus selectively degrades toxic inhibitors generated from pretreatment of lignocellulose without consuming fermentable sugars.However,one barrier for practical application is the sustained cell via...Biodetoxification fungus selectively degrades toxic inhibitors generated from pretreatment of lignocellulose without consuming fermentable sugars.However,one barrier for practical application is the sustained cell viability in the consequent fermentation step to compete the fermentable sugars with fermenting strains,resulting in sugar loss and reduced target product yield.This study investigated the competitive growth property between the biodetoxification fungus Paecilomyces variotii FN89 and the L-lactic acid bacterium Pediococcus acidilactici ZY271 under varying temperature and lactic acid osmatic stress.The results show that the L-lactic acid bacterium Ped.acidilactici ZY271 showed less thermotolerance to Pae.variotii FN89 at high temperature of 45°C to 50°C in both synthetic medium and wheat straw hydrolysate.In the higher temperature environment,the growth of the biodetoxification strian failed to compete with the lactic acid fermentation strain and was quickly eliminated from the fermentation system.The high temperature fermentation facilitated a fast transition from the detoxification stage to the fermentation stage for higher production of L-lactic acid.展开更多
Microbial fermentation could produce many active substances in green and pollution-free ways.In recent years,studies on the hypoglycemic ability of microorganisms had become a trending topic in the prevention and trea...Microbial fermentation could produce many active substances in green and pollution-free ways.In recent years,studies on the hypoglycemic ability of microorganisms had become a trending topic in the prevention and treatment of diabetes.Garlic is a food containing a variety of biologically active substances and physiological functions,but it is not accepted by the general public because of its strong pungent smell.In our study,the microorganisms with potential hypoglycemic effect were screened from strains resistant to garlic juice.As a result,41 strains of Bacillus,157 strains of lactic acid bacteria,and 23 strains of yeast with tolerance of garlic juice were screened from the selected 568 strains.After primary screening ofα-amylase inhibitor,it was found that 15 Bacillus and 9 lactic acid bacteria had highα-amylase inhibitory activity.Besides,afterα-glucosidase inhibitor re-screening,Pediococcus acidilactici Z1 with highα-amylase inhibitory activity andα-glucosidase inhibitory activity was screened out.The results of fermented garlic showed that the inhibition rate ofα-amylase andα-glucosidase of garlic from different origins were significantly increased from 59.09 to 63.99%(p<0.01)and from 52.71 to 71.93%(p<0.01)after Z1 fermentation,respectively.The improvement rates ofα-amylase andα-glucosidase were 107.69 to 287.32%,and 197.74 to 239.86%,respectively.In a word,this study provides a theoretical and experimental basis for the development of new functional garlic products.展开更多
Germination,elicitation,and fermentation are biotechnological methods that could be applied to improve phytochemical profile and functional quality of edible seeds.This research investigated the combined effects of el...Germination,elicitation,and fermentation are biotechnological methods that could be applied to improve phytochemical profile and functional quality of edible seeds.This research investigated the combined effects of elicitation,germination,and fermentation on the anti-obesity activities of alfalfa seeds.Alfalfa seeds were germinated at 25-27℃ for 3 days while spraying with tap water(control)or 1%of different plant elicitors(sodium chloride,citric acid,or ascorbic acid)solution.Ascorbic acid was the most suitable elicitor to improve phytochemical quality,support growth,and maximize the health-promoting properties of alfalfa sprouts.Treatment with 1%ascorbic acid solution improved lipase(74.57%vs.41.32%),DPPH(76.58%vs.57.21%),and ABTS(71.63%vs.46.5%)inhibitory activities of alfalfa sprouts compared to control.Fermentation of the ascorbic acid-elicited sprouts further enhanced the anti-obesity activities in vitro by improving their inhibitory potential against digestive enzymes,alpha-glucosidase(67.84%vs.81.39%)and pancreatic lipase(74.57%vs.82.07%).The fermented sprout extracts prolonged the lifespan and decreased fat accumulation in the glucose-induced obese Caenorhabditis elegans via mechanisms including suppression of genes involved in fat synthesis and changing the worms’metabolite profiles.Specifically,the extract down-regulated genes involved in fat synthesis(fat-4,fat-6 and fat-7),up-regulated genes for fat lipolysis(hosl-1)and lifespan gene(daf-16),while enhancing amino acids profiles in C.elegans.These findings demonstrate that alfalfa sprouts produced through a combination of ascorbic acid treatment and fermentations could be an effective functional ingredient targeting obesity.展开更多
基金supported by the National Natural Science Foundation of China Program(32021005)the Natural Science Foundation of Jiangsu Province(BK20200084)+2 种基金Projects of Innovation and Development Pillar Program for Key Industries in Southern Xinjiang of Xinjiang Production and Construction Corps(2018DB002)National First Class Discipline Program of Food Science and Technology(JUFSTR20180102)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici strains with antibacterial activity(P.acidilactici CCFM28 and CCFM18)were obtained based on the inhibition-zone assay.The produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide treatment.Bacteriocin-producing and non-bacteriocin-producing P.acidilactici strains(P.acidilactici CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal mice.After 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum level.At the genus level,the administration of three P.acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus.Furthermore,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of strain.The results indicated that the intervention of different P.acidilactici strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.
基金supported by Exploring and Overturning Innovation Program of Jiangsu Academy of Agricultural Sciences(ZX(21)1225)Key R&D Program Project of the Autonomous Region(2023B02028-2)。
文摘In this study,α-amylase andα-glucosidase inhibition activities of cell-free supernatant(CFS)of 216 lactic acid bacteria were measured to screen lactic acid bacteria resources with potential hypoglycemic functions.Significant inhibition activities were detected in Pediococcus acidilactici FM-Pa-JXM94,L15,GC215 and L31,and their probiotic properties were further assessed.Following the inhibitory activities onα-glucosidase andα-amylase,the viability of simulated gastric juice and adhesive ability,FM-Pa-JXM94 was screened and identified as Pediococcus acidilactici by 16S rDNA nucleotide sequencing,and the potential hypoglycemic mechanism was explored using IR-HepG2.CFS,cell-free extract(CFE)and bacterial cells of FM-Pa-JXM94 demonstrated a strong potential to induce glucose consumption,glycogen synthesis,and pyruvate kinase activity,as well as decrease phosphoenolpyruvate carboxykinase activity of IR-HepG2 cells.Additionally,RT-qPCR results showed that CFS,CFE and bacteria cells upregulated the mRNA expression of IRS1,AKT,GYS2 and PI3K genes and downregulated the mRNA expression of GSK3β,FOXO1,PEPCK and G-6-Pase genes of IRS-1/PI3K/AKT/GSK3β/GYS2 and PI3K/AKT/FOXO1/PEPCK-G6Pase signal pathways.These findings illustrate that the different FM-Pa-JXM94 derivatives could alleviate insulin resistance of IR-HepG2 cells from key enzyme activity and gene levels.This study showcases FM-Pa-JXM94 as a promising probiotic candidate for preparing functional foods and hypoglycemic function supplements.
基金financially supported by the National Natural Science Foundation of China(32201988)the China Postdoctoral Science Foundation(2025M772993)+3 种基金the Postdoctoral Fellowship Program of CPSF(GZC20251966)the Jiangsu Funding Program for Excellent Postdoctoral Talent(2025ZB158)the Fundamental Research Funds for the Central Universities(JUSRP123047)the Program of Collabo-rative Innovation Centre of Food Safety and Quality Control in Jiangsu Province.
文摘Maternal health plays a crucial role in fetal neurodevelopment,with high-fat diet(HFD)consumption before or during pregnancy known to induce neurodevelopmental and behavioral abnormalities in offspring.In this study,we assessed whether supplementation with Pediococcus acidilactici CCFM1344(10^(9) CFU/day,oral gavage)during pregnancy in C57BL/6J mice could serve as an effective strategy to counteract neurodevelopmental impairments in offspring induced by maternal HFD.Female mice(8 weeks old)were used for breeding,and both male and female offspring were included in the analyses.16S rRNA gene sequencing revealed that CCFM1344 significantly modulated the maternal gut microbiota composition,reduced the Firmicutes-to-Bacteroidetes(F/B)ratio,and enriched beneficial genera Alloprevotella and Rikenellaceae RC9 gut group.Behavioral assessments,including the open field and three-chamber social interaction tests,demonstrated that CCFM1344 effectively ameliorated hyperactivity and social deficits in juvenile offspring,with these behavioral changes showing partial normalization in adulthood.Gut microbiota analysis of the juvenile offspring indicated that CCFM1344 significantly affected microbial diversity and improved ecological stability.In mothers,fecal metabolomics and serum cytokine profiling showed that CCFM1344 intervention regulated key metabolites,including taurine and Lhistidine,and modulated inflammatory markers such as TNF-α,IFN-γ,and IL-10.In addition,transcriptomic and quantitative real-time PCR analyses of neonatal brain tissue revealed that CCFM1344 reversed the abnormal expression of genes associated with neuroinflammation and myelination—including Gfap,Mmp9,Il-1β,and Mobp—with these changes normalizing by adulthood.In conclusion,pregnancy-specific administration of CCFM1344 modulated the maternal gut microbiota and immunometabolic environment,alleviating HFDinduced impairments in early brain development and behavior in offspring.These findings highlight the critical importance of probiotic interventions during pregnancy in supporting neurodevelopmental health in the next generation.
文摘The immune system plays a crucial role in maintaining host defense and homeostasis through complex cellular and molecular interactions.This study evaluated the immunostimulatory effects of the paraprobiotic Pediococcus acidilactici SY23 in combination with chondroitin sulfate(CS)on RAW 264.7 murine macrophages.Co-treatment significantly enhanced cell viability and upregulated the production of nitric oxide(NO)and prostaglandin E2(PGE2)compared to individual treatments.In addition,phagocytic activity and intracellular reactive oxygen species(ROS)levels were markedly elevated.Co-treatment also induced substantial increases in the mRNA levels of pro-inflammatory cytokines,including interleukin(IL)-1β,IL-6,and tumor necrosis factor-α(TNF-α).The observed immunostimulatory responses were linked to the activation of nuclear factor-kappa B(NF-κB)and mitogen-activated protein kinase(MAPK)signaling cascades,as demonstrated by elevated phosphorylation levels of p65,ERK,JNK,and p38.Furthermore,the expression of inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)was markedly upregulated following co-treatment.These findings demonstrate that paraprobiotic P.acidilactici SY23,in combination with CS,promotes macrophage-mediated immune activation via the NF-κB and MAPK pathways.This suggests potential applicability of the formulation as a safe and effective immunomodulatory agent in the development of functional foods or nutraceuticals.
基金supported by the National Key R&D Program of China(2023YFC2506004)National Natural Science Foundation of China(32201988)the Fundamental Research Funds for the Central Uni-versities(JUSRP123047).
文摘Pediococcus acidilactici has demonstrated promising potential as a psychobiotic with positive effects on gut health and depressive behaviors.Prior research has found that the relative levels of lactate in the intestines of both depressed patients and mice are different from normal levels.In this study,we established a chronic unpredictable mild stress mouse model to compare the effects of high-lactate-producing P.acidilactici strain CCFM1344 and low-lactate-producing strain FSDLZ42M3 on depressive symptoms.Our experimental results showed that after four weeks of intervention,CCFM1344 significantly alleviated anxiety-like and depression-like behaviors induced by CUMS.Furthermore,CCFM1344 restored serum LPS levels,as well as the abnormally elevated LPS receptor protein and downstream pro-inflammatory cytokine genes expression in the hippocampus.Conversely,FSDLZ42M3 did not exhibit these effects.Notably,CCFM1344 was found to inhibit the abnormal activation of microglia for the first time.Additionally,16S rDNA V3-V4 sequencing and untargeted metabolomics assays revealed that CCFM1344 intervention improved gut microbiota dysbiosis.Specifically,it increased the relative abundance of the Lachnospiraceae NK4A136 group and regulated the aberrant intestinal metabolites in mice.These findings expand the potential applications of P.acidilactici as a probiotic and provide theoretical guidance for using CCFM1344 to prevent,alleviate,and treat neurological dysfunction.
基金supported by the Key Special Projects of the Ministry of Science and Technology(SQ2020YFF0404523)the North Anhui Soybean Advantageous Characteristic Industry Cluster Project(2023CYJQ013)+2 种基金the National Natural Science Foundation of China(32172162)the Key Genetic Technologies Research and Development Program of Hefei(2021GJ075)the Young Talents Program of Anhui Academy of Agricultural Science(QNYC-202122).
文摘Okara is produced in large quantities annually in China,but much of it is discarded due to its high content of indigestible dietary fiber(DF),contributing to significant environmental challenges.Recognizing the underexplored medicinal potential of DF,we developed an efficient fermentation method to enhance the bioavailability of okara fiber.In this study,Pediococcus acidilactici IFJ-1,which has strong enzymatic production capabilities and beneficial effects on gastrointestinal flora modulation,was selected to ferment okara.Results showed decreases in viscosity and particle size,optimized surface structure,improved thermal stability and hydration properties,and a significant increase in soluble DF content from 1.85%to 3.91%.To evaluate the physiological effects,hyperlipidemic mouse models were established and subjected to dietary interventions utilizing okara and fermented okara to measure changes in physicochemical parameters,gut microbiota composition,and lipid metabolism.The dietary intervention was effective,particularly in the fermented okara group,showing a 7.3%weight loss,improved blood lipids(triglycerides:‒39.8%,total cholesterol:‒12.8%,low-density lipoprotein cholesterol:‒34.2%,high-density lipoprotein cholesterol:+26.2%),and a 22.2%lower liver index.Gut microbiota analysis revealed that fermented okara positively modulated the microbial community by increasing the abundance of beneficial bacteria(e.g.,Bacteroidota)and reducing the abundance of obesity-associated bacteria(e.g.,Bacillota).Lipid metabolism profiling further demonstrated that fermented okara downregulated harmful lipids(e.g.,(O-acyl)-ω-hydroxy fatty acids,ceramides,and diacylglycerols)while upregulating beneficial phospholipids(e.g.,phosphatidylinositol,phosphatidylserine,phosphatidylethanolamine,lysophosphatidylinositol and lysophosphatidic acid).This study highlights a novel approach for enhancing DF utilization through fermentation,providing valuable insights into strategies for preventing obesity and metabolic diseases.
基金The National Key R&D Program of China(2023YFA0914400)the National Natural Science Foundation of China(32301269)+1 种基金China Postdoctoral Science Foundation(2023M741175)the Yangfan Project of Science and Technology Committee of Shanghai Municipality(23YF1409900).
文摘Biodetoxification fungus selectively degrades toxic inhibitors generated from pretreatment of lignocellulose without consuming fermentable sugars.However,one barrier for practical application is the sustained cell viability in the consequent fermentation step to compete the fermentable sugars with fermenting strains,resulting in sugar loss and reduced target product yield.This study investigated the competitive growth property between the biodetoxification fungus Paecilomyces variotii FN89 and the L-lactic acid bacterium Pediococcus acidilactici ZY271 under varying temperature and lactic acid osmatic stress.The results show that the L-lactic acid bacterium Ped.acidilactici ZY271 showed less thermotolerance to Pae.variotii FN89 at high temperature of 45°C to 50°C in both synthetic medium and wheat straw hydrolysate.In the higher temperature environment,the growth of the biodetoxification strian failed to compete with the lactic acid fermentation strain and was quickly eliminated from the fermentation system.The high temperature fermentation facilitated a fast transition from the detoxification stage to the fermentation stage for higher production of L-lactic acid.
基金supported by the program of Key Laboratory of Industrial Biotechnology of Ministry of Education in Jiangnan University(KLIB-KF202105)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)+1 种基金Program of Introducing Talents of Discipline to Universities(No.111-2-06)the National High Technology Research and Development Program 863(No.2018YFD0400403).
文摘Microbial fermentation could produce many active substances in green and pollution-free ways.In recent years,studies on the hypoglycemic ability of microorganisms had become a trending topic in the prevention and treatment of diabetes.Garlic is a food containing a variety of biologically active substances and physiological functions,but it is not accepted by the general public because of its strong pungent smell.In our study,the microorganisms with potential hypoglycemic effect were screened from strains resistant to garlic juice.As a result,41 strains of Bacillus,157 strains of lactic acid bacteria,and 23 strains of yeast with tolerance of garlic juice were screened from the selected 568 strains.After primary screening ofα-amylase inhibitor,it was found that 15 Bacillus and 9 lactic acid bacteria had highα-amylase inhibitory activity.Besides,afterα-glucosidase inhibitor re-screening,Pediococcus acidilactici Z1 with highα-amylase inhibitory activity andα-glucosidase inhibitory activity was screened out.The results of fermented garlic showed that the inhibition rate ofα-amylase andα-glucosidase of garlic from different origins were significantly increased from 59.09 to 63.99%(p<0.01)and from 52.71 to 71.93%(p<0.01)after Z1 fermentation,respectively.The improvement rates ofα-amylase andα-glucosidase were 107.69 to 287.32%,and 197.74 to 239.86%,respectively.In a word,this study provides a theoretical and experimental basis for the development of new functional garlic products.
基金funded by the Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ0145652021)Rural Development Administration,Korea.
文摘Germination,elicitation,and fermentation are biotechnological methods that could be applied to improve phytochemical profile and functional quality of edible seeds.This research investigated the combined effects of elicitation,germination,and fermentation on the anti-obesity activities of alfalfa seeds.Alfalfa seeds were germinated at 25-27℃ for 3 days while spraying with tap water(control)or 1%of different plant elicitors(sodium chloride,citric acid,or ascorbic acid)solution.Ascorbic acid was the most suitable elicitor to improve phytochemical quality,support growth,and maximize the health-promoting properties of alfalfa sprouts.Treatment with 1%ascorbic acid solution improved lipase(74.57%vs.41.32%),DPPH(76.58%vs.57.21%),and ABTS(71.63%vs.46.5%)inhibitory activities of alfalfa sprouts compared to control.Fermentation of the ascorbic acid-elicited sprouts further enhanced the anti-obesity activities in vitro by improving their inhibitory potential against digestive enzymes,alpha-glucosidase(67.84%vs.81.39%)and pancreatic lipase(74.57%vs.82.07%).The fermented sprout extracts prolonged the lifespan and decreased fat accumulation in the glucose-induced obese Caenorhabditis elegans via mechanisms including suppression of genes involved in fat synthesis and changing the worms’metabolite profiles.Specifically,the extract down-regulated genes involved in fat synthesis(fat-4,fat-6 and fat-7),up-regulated genes for fat lipolysis(hosl-1)and lifespan gene(daf-16),while enhancing amino acids profiles in C.elegans.These findings demonstrate that alfalfa sprouts produced through a combination of ascorbic acid treatment and fermentations could be an effective functional ingredient targeting obesity.
