[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect cal...[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS ＋ 2.0 - 3.0 mg/L 6-BA ＋ 0.2 - 0.3 mg/L NAA, MS ＋ 1.0 - 2.0 mg/L 6-BA ＋ 0.1 - 0.2 mg/L NAA, MS and 1/2MS ＋ 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.展开更多
Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that dir...Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that directly affect fruit appearance and plant management.This study used RNA sequencing technology to analyze the transcriptomes of two parts of the inflorescence branch,namely inflorescence stems with flagellated trichomes and pedicels with both flagellated and glandular trichomes.Comparative transcriptomic analysis showed that many transcription factors(TFs)are potentially involved in the formation and development of trichomes.The accumulation of RsETC1,a TF of the R3-MYB family,was significantly higher in inflorescence stems than in pedicels;quantitative reverse transcription PCR(qRTPCR)verified that its expression was significantly higher in inflorescence stems than in pedicels during the first three development stages,indicating its inhibitory action on the initiation of glandular trichomes in R.sterilis.The mRNA level of RsETC1 accumulated to significantly higher levels in trichomeless tissues than in tissues with trichromes,suggesting that this gene may inhibit the formation of trichomes in R.sterilis.Over-expression of RsETC1 in Arabidopsis resulted in glabrous phenotypes,and the expression of trichome-related endogenous genes,except for TTG1,was markedly reduced.In addition,the contents of the phytohormones jasmonic acid(JA),gibberellin A3(GA_(3)),and cytokinins(CKs)in pedicels were significantly higher than those in inflorescence stems,and the expression patterns of the genes related to hormone biosynthesis and signal transduction presented consistent responses,suggesting that the transduction of these hormones might be crucial for trichome initiation and development.These data provide a new perspective for revealing the molecular mechanism of trichome formation in R.sterilis.展开更多
Objective:To investigate the application value of 3D printing technique in the operation of the lumbar isthmic spondylolisthesis.Methods Totally 48 patients with lumbar isthmic spondylolisthesis treated in our departm...Objective:To investigate the application value of 3D printing technique in the operation of the lumbar isthmic spondylolisthesis.Methods Totally 48 patients with lumbar isthmic spondylolisthesis treated in our department from January 2015 to April 2017 were selected in the research plan.According to the random digital table method,they were divided into the observation group and the control group,with 25 cases in the observation group and 23 cases in control group.All patients were treated with transforaminal lumbar interbody fusion(TLIF).The control group made the observation group made the operation plan with 3D printing technology,and the operation plan according to the 3D CT reconstruction image.The clinical and imaging results were compared between the two groups、including operation time、intraoperative blood loss and X-ray exposure、complications、preoperative and postoperative ODI and JOA score.Results The accuracy of the screw placement in the observation group was 95.33%,which was significantly higher than those of 84.06%in the control group,the differences were statistically significant(P<0.05).Operation time and X-ray exposure and intraoperative blood loss in observation group were less than those in the control group,the differences were statistically significant(P<0.05).There were no significant differences spondylolisthesis reduction degree、ODI and JOA scores at postoperative different point between groups(P>0.05).Conclusions 3D printing technology is helpful to the development of the lumbar isthmic spondylolisthesis surgical plan,with the advantages of shortening the operation time and X-ray exposure,reducing the blood loss,improving the accuracy of the screw placement.展开更多
The results of a comparative study of the structure of some vegetative organs of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdan...The results of a comparative study of the structure of some vegetative organs of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">caspium</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">tschimganicum</span></i><span style="font-family:Verdana;"> growing in natural conditions in Uzbekistan are presented for the first time. During the study of the desert representative </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">caspium</span></i><span style="font-family:Verdana;">, the following diagnostic, structural features were revealed: ribbing of the peduncle and pedicel;thickening of the outer wall of the epidermis and wavy folding of the cuticle of the parenchymal-bundle type of structure</span><span><span style="font-family:Verdana;">;deep submergence of stomata: extensive core: </span><span style="font-family:Verdana;">thinness</span><span style="font-family:Verdana;"> of the crustal parenchyma, the presence of lactic acid in it;the presence of a highly </span><span style="font-family:Verdana;">sclerified</span> <span style="font-family:Verdana;">sclerinchymal</span><span style="font-family:Verdana;"> ring between the crustal parenchyma and the central cylinder: the vastness of the core and the presence of </span><span style="font-family:Verdana;">hydrocytic</span><span style="font-family:Verdana;"> cells in it, as well as the similarity of the structure of </span></span><span style="font-family:Verdana;">the stem and pedicel. In this species, a predominance of xeromorphic characters was noted, due to a tendency indicating </span><span style="font-family:Verdana;">xerophilization</span><span style="font-family:Verdana;"> and adaptation to desert conditions. In </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">tschimganicum</span></i><span style="font-family:Verdana;">, the following mesomorphic diagnostic structural features, opposite to the desert one, were also revealed: </span><span style="font-family:Verdana;">roundness</span><span style="font-family:Verdana;"> of the stem and pedicel, dense folding of the cuticle surface, the presence of lactates in the crustal parenchyma, weak thickening of the crustal parenchyma, and the presence of a weakly </span><span style="font-family:Verdana;">sclerized</span> <span style="font-family:Verdana;">sclerenchymal</span><span style="font-family:Verdana;"> ring in the central color parenchyma: </span><span style="font-family:Verdana;">parenchymal-bundle</span><span style="font-family:Verdana;"> type of structure, weak thickening of the outer wall of the epidermis and slightly waviness of the cuticle surface, the presence of lactic acid in the crustal parenchyma. Weak submergence of stomata, large-cell crustal parenchyma, extensive, large-cell, thin-walled, poorly </span><span style="font-family:Verdana;">sclerified</span><span style="font-family:Verdana;"> central cylinder, also similarity to the structure of the stem with pedicel, but the presence of a cavity in the stem. The predominance of mesomorphic traits in this endemic species indicat</span></span><span style="font-family:Verdana;">ed</span><span style="font-family:Verdana;"> more favorable</span><span style="font-family:""> </span><span style="font-family:Verdana;">mountain humid growing conditions w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> noted.展开更多
Hundreds of leucine-rich repeat receptor-like kinases （LRR-RLKs） play indispensable roles in a wide range of plant developmental and physiological processes. The mechanisms controlling LRR-RLKs at a basal and inacti...Hundreds of leucine-rich repeat receptor-like kinases （LRR-RLKs） play indispensable roles in a wide range of plant developmental and physiological processes. The mechanisms controlling LRR-RLKs at a basal and inactive status are essential but rarely studied. BKI1 is the only reported inhibitor of receptor kinases in Arabidopsis, which negatively regulates BRI1 in the brassinosteroid pathway. In this study, we found that BKI1 can also interact with another important LRR-RLK, ERECTA （ER）. Phenotypic analysis showed that BKI1 and ER together regulate plant architecture, including pedicel orientation, which is a newly reported phenotype in the BR- and ER-mediated developmental processes. Gene expression analysis revealed that BKI1 regulates a subset of ER-responsive genes. Kinase assays demonstrated that BKI1 inhibits ER kinase activity. In addition, the release of BKI1 inhibition on ER signaling relies largely on BRI1 activation. Our data provide significant insights into the regulation and activation of RLKs and suggest that BKI1 functions as a common suppressor of the BRI1 and ER signaling pathways.展开更多
基金Supported by China Agricultural University (Yantai) Project(yt2007.14)
文摘[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS ＋ 2.0 - 3.0 mg/L 6-BA ＋ 0.2 - 0.3 mg/L NAA, MS ＋ 1.0 - 2.0 mg/L 6-BA ＋ 0.1 - 0.2 mg/L NAA, MS and 1/2MS ＋ 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.
基金supported by grants from the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province,China(U1812401)the Talent Project of Guizhou Province,China(20164016)。
文摘Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that directly affect fruit appearance and plant management.This study used RNA sequencing technology to analyze the transcriptomes of two parts of the inflorescence branch,namely inflorescence stems with flagellated trichomes and pedicels with both flagellated and glandular trichomes.Comparative transcriptomic analysis showed that many transcription factors(TFs)are potentially involved in the formation and development of trichomes.The accumulation of RsETC1,a TF of the R3-MYB family,was significantly higher in inflorescence stems than in pedicels;quantitative reverse transcription PCR(qRTPCR)verified that its expression was significantly higher in inflorescence stems than in pedicels during the first three development stages,indicating its inhibitory action on the initiation of glandular trichomes in R.sterilis.The mRNA level of RsETC1 accumulated to significantly higher levels in trichomeless tissues than in tissues with trichromes,suggesting that this gene may inhibit the formation of trichomes in R.sterilis.Over-expression of RsETC1 in Arabidopsis resulted in glabrous phenotypes,and the expression of trichome-related endogenous genes,except for TTG1,was markedly reduced.In addition,the contents of the phytohormones jasmonic acid(JA),gibberellin A3(GA_(3)),and cytokinins(CKs)in pedicels were significantly higher than those in inflorescence stems,and the expression patterns of the genes related to hormone biosynthesis and signal transduction presented consistent responses,suggesting that the transduction of these hormones might be crucial for trichome initiation and development.These data provide a new perspective for revealing the molecular mechanism of trichome formation in R.sterilis.
基金National Natural Science Foundation of China(81641136,81703659).
文摘Objective:To investigate the application value of 3D printing technique in the operation of the lumbar isthmic spondylolisthesis.Methods Totally 48 patients with lumbar isthmic spondylolisthesis treated in our department from January 2015 to April 2017 were selected in the research plan.According to the random digital table method,they were divided into the observation group and the control group,with 25 cases in the observation group and 23 cases in control group.All patients were treated with transforaminal lumbar interbody fusion(TLIF).The control group made the observation group made the operation plan with 3D printing technology,and the operation plan according to the 3D CT reconstruction image.The clinical and imaging results were compared between the two groups、including operation time、intraoperative blood loss and X-ray exposure、complications、preoperative and postoperative ODI and JOA score.Results The accuracy of the screw placement in the observation group was 95.33%,which was significantly higher than those of 84.06%in the control group,the differences were statistically significant(P<0.05).Operation time and X-ray exposure and intraoperative blood loss in observation group were less than those in the control group,the differences were statistically significant(P<0.05).There were no significant differences spondylolisthesis reduction degree、ODI and JOA scores at postoperative different point between groups(P>0.05).Conclusions 3D printing technology is helpful to the development of the lumbar isthmic spondylolisthesis surgical plan,with the advantages of shortening the operation time and X-ray exposure,reducing the blood loss,improving the accuracy of the screw placement.
文摘The results of a comparative study of the structure of some vegetative organs of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">caspium</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">tschimganicum</span></i><span style="font-family:Verdana;"> growing in natural conditions in Uzbekistan are presented for the first time. During the study of the desert representative </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">caspium</span></i><span style="font-family:Verdana;">, the following diagnostic, structural features were revealed: ribbing of the peduncle and pedicel;thickening of the outer wall of the epidermis and wavy folding of the cuticle of the parenchymal-bundle type of structure</span><span><span style="font-family:Verdana;">;deep submergence of stomata: extensive core: </span><span style="font-family:Verdana;">thinness</span><span style="font-family:Verdana;"> of the crustal parenchyma, the presence of lactic acid in it;the presence of a highly </span><span style="font-family:Verdana;">sclerified</span> <span style="font-family:Verdana;">sclerinchymal</span><span style="font-family:Verdana;"> ring between the crustal parenchyma and the central cylinder: the vastness of the core and the presence of </span><span style="font-family:Verdana;">hydrocytic</span><span style="font-family:Verdana;"> cells in it, as well as the similarity of the structure of </span></span><span style="font-family:Verdana;">the stem and pedicel. In this species, a predominance of xeromorphic characters was noted, due to a tendency indicating </span><span style="font-family:Verdana;">xerophilization</span><span style="font-family:Verdana;"> and adaptation to desert conditions. In </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i> <span style="font-family:Verdana;">tschimganicum</span></i><span style="font-family:Verdana;">, the following mesomorphic diagnostic structural features, opposite to the desert one, were also revealed: </span><span style="font-family:Verdana;">roundness</span><span style="font-family:Verdana;"> of the stem and pedicel, dense folding of the cuticle surface, the presence of lactates in the crustal parenchyma, weak thickening of the crustal parenchyma, and the presence of a weakly </span><span style="font-family:Verdana;">sclerized</span> <span style="font-family:Verdana;">sclerenchymal</span><span style="font-family:Verdana;"> ring in the central color parenchyma: </span><span style="font-family:Verdana;">parenchymal-bundle</span><span style="font-family:Verdana;"> type of structure, weak thickening of the outer wall of the epidermis and slightly waviness of the cuticle surface, the presence of lactic acid in the crustal parenchyma. Weak submergence of stomata, large-cell crustal parenchyma, extensive, large-cell, thin-walled, poorly </span><span style="font-family:Verdana;">sclerified</span><span style="font-family:Verdana;"> central cylinder, also similarity to the structure of the stem with pedicel, but the presence of a cavity in the stem. The predominance of mesomorphic traits in this endemic species indicat</span></span><span style="font-family:Verdana;">ed</span><span style="font-family:Verdana;"> more favorable</span><span style="font-family:""> </span><span style="font-family:Verdana;">mountain humid growing conditions w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> noted.
文摘Hundreds of leucine-rich repeat receptor-like kinases （LRR-RLKs） play indispensable roles in a wide range of plant developmental and physiological processes. The mechanisms controlling LRR-RLKs at a basal and inactive status are essential but rarely studied. BKI1 is the only reported inhibitor of receptor kinases in Arabidopsis, which negatively regulates BRI1 in the brassinosteroid pathway. In this study, we found that BKI1 can also interact with another important LRR-RLK, ERECTA （ER）. Phenotypic analysis showed that BKI1 and ER together regulate plant architecture, including pedicel orientation, which is a newly reported phenotype in the BR- and ER-mediated developmental processes. Gene expression analysis revealed that BKI1 regulates a subset of ER-responsive genes. Kinase assays demonstrated that BKI1 inhibits ER kinase activity. In addition, the release of BKI1 inhibition on ER signaling relies largely on BRI1 activation. Our data provide significant insights into the regulation and activation of RLKs and suggest that BKI1 functions as a common suppressor of the BRI1 and ER signaling pathways.
