[Objective]The paper was to screen the effective agents for effectively controlling the occurrence and prevalence of taro soft rot.[Method]The fungicidal effects of 11 fungicides on taro soft rot were determined by in...[Objective]The paper was to screen the effective agents for effectively controlling the occurrence and prevalence of taro soft rot.[Method]The fungicidal effects of 11 fungicides on taro soft rot were determined by inhibition zone method.[Result]0.3%Tetramycin,25%bromothalonil,3%Zhongshengmycin and 30%zinc thiazole had better antifungal effect on Pectobacterium aroidearum at recommended concentration.The indoor toxicity of four fungicides showed that the EC50 of 0.3%tetramycin AS was the smallest of 0.6μg/mL,indicating that tetramycin had the largest toxicity and the best inhibitory effect against P.aroidearum;followed by 25%bromothalonil EC and 3%Zhongshengmycin WP,with the EC_(50) of 2.57 and 97.72μg/mL,respectively;the inhibitory effect of 30%zinc thiazole SC against P.aroidearum was the poorest.[Conclusion]The study provides a reference for screening out new and efficient chemical agents against taro soft rot.展开更多
Konjac(Amorphophallus konjac)is an important food crop and a traditional medicinal herb in China.However,bacterial soft rot poses a serious threat to konjac production and quality.In this study,we identified Pectobact...Konjac(Amorphophallus konjac)is an important food crop and a traditional medicinal herb in China.However,bacterial soft rot poses a serious threat to konjac production and quality.In this study,we identified Pectobacterium aroidearum as the causative pathogen of soft rot in konjac and investigated the antibacterial effects of kasugamycin(KSM)against this bacterium.Our results show that KSM exhibits strong antibacterial activity,significantly reducing the virulence of P.aroidearum and providing both protective and curative effects against konjac soft rot.Further mechanistic analysis reveal that KSM disrupts key gene expression associated with membrane transport in P.aroidearum,leading to inhibited swimming motility,reduced production of virulence factors,and significant alterations in cell morphology.These findings highlight the antibacterial properties of KSM against P.aroidearum,offering valuable insights into its potential application for treating bacterial soft rot in konjac.展开更多
基金Supported by Research and Demonstration of Key Technologies for Rhizome Disease Control in Yimeng Tobacco Area(201906)Science and Technology Innovation Project of Chinese Academy of Agri-cultural Sciences(ASTIP-TRIC04)Demonstration of Green Degradation Technology and Resource Utilization of Tobacco Straw(202013)。
文摘[Objective]The paper was to screen the effective agents for effectively controlling the occurrence and prevalence of taro soft rot.[Method]The fungicidal effects of 11 fungicides on taro soft rot were determined by inhibition zone method.[Result]0.3%Tetramycin,25%bromothalonil,3%Zhongshengmycin and 30%zinc thiazole had better antifungal effect on Pectobacterium aroidearum at recommended concentration.The indoor toxicity of four fungicides showed that the EC50 of 0.3%tetramycin AS was the smallest of 0.6μg/mL,indicating that tetramycin had the largest toxicity and the best inhibitory effect against P.aroidearum;followed by 25%bromothalonil EC and 3%Zhongshengmycin WP,with the EC_(50) of 2.57 and 97.72μg/mL,respectively;the inhibitory effect of 30%zinc thiazole SC against P.aroidearum was the poorest.[Conclusion]The study provides a reference for screening out new and efficient chemical agents against taro soft rot.
基金supported by grants from the National Natural Science Foundation of China(32300164,32460045)Guizhou Provincial Basic Research Program(Natural Science)(Qian Ke He Ji Chu-ZK[2023]General 095)+2 种基金Natural Science Special Research Fund of Guizhou University,Special Post([2022]50)Guizhou Highland Specialty Vegetable Green Production Science and Technology Innovation Talent Team(Qian Ke He Platform Talent-CXTD[2022]003)Plateau Characteristic Vegetable Industry Technology System of Guizhou Province(GZGYTSSCCYJSTX-04).
文摘Konjac(Amorphophallus konjac)is an important food crop and a traditional medicinal herb in China.However,bacterial soft rot poses a serious threat to konjac production and quality.In this study,we identified Pectobacterium aroidearum as the causative pathogen of soft rot in konjac and investigated the antibacterial effects of kasugamycin(KSM)against this bacterium.Our results show that KSM exhibits strong antibacterial activity,significantly reducing the virulence of P.aroidearum and providing both protective and curative effects against konjac soft rot.Further mechanistic analysis reveal that KSM disrupts key gene expression associated with membrane transport in P.aroidearum,leading to inhibited swimming motility,reduced production of virulence factors,and significant alterations in cell morphology.These findings highlight the antibacterial properties of KSM against P.aroidearum,offering valuable insights into its potential application for treating bacterial soft rot in konjac.
