Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharide...Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharides.This study extracts pectic polysaccharides from goji berry,raspberry,and cranberry,examines how HPP influences the pectic polysaccharide structure of three berries,and provides a basis for the extraction and modification of pectic polysaccharides with specific structure and bioactivity.Materials and methods:An orthogonal test was performed to optimize the HPP-assisted alkali method to extract the high yield and high RG-I content pectic polysaccharides from three berries.Structural information of pectic polysaccharides extracted by the HPP method and conventional methods were compared from the perspectives of monosaccharide composition,molecular weight,Fourier transform infrared spectroscopy,andnuclearmagneticresonancespectroscopy.Results:For raspberry,the optimal conditions consisted of a pressure of 50o MPa,a pH of 13,and a pressure-holding time of 12 min,while the optimal conditions for goji berry and cranberry were both 40o MPa,pH 13,and 15 min.Under the optimal conditions,the yields for goji berry,raspberry,and cranberry were 10.49%,16.63%,and 17.52%,respectively,and RG-l contents were 81.85%,83.30%,and 63.22%,respectively.HPP showed an effect to degrade homogalacturonan backbones and side chains and increase the RG-I content to some extent.Conclusion:HPP-assisted alkali method was revealed to be an efficient method to extract high RG-I content pectic polysaccharides,especially for cranberry,and was a potential method to modify pectic polysaccharide structure in a certain way.展开更多
AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar...AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management.展开更多
In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude...In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude polysaccharides(SCP)were extracted using hot water extraction and ethanol precipitation.SCP is composed primarily of eight monosaccharides,including galacturonic acid(46.6%),galactose(10.4%),arabinose(5.6%),and rhamnose(3.8%),that are typical sugar components of pectic polysaccharides.Oral administration of SCP recovered body weight loss and significantly alleviated various ulcerative colitis symptoms such as stool consistency,blood in stool,and blood in the anus.Additionally,the DSS-treated group that exhibited a decrease in colon length,and SCP treatment significantly reduced colon length shortening in a dose-dependent manner.Levels of inflammatory factors such as interleukin-6,tumor necrosis factor-α,and myeloperoxidase were lower in the SCP-treated groups than they were in the DSS-treated group.In contrast,Alcian blue-periodic acid Schiff staining revealed that SCP treatment effectively maintained the amount of mucus and goblet cells in the intestinal lumen compared to that in the DSS-treated group.The short-chain fatty acid content decreased by DSS administration was recovered through oral administration of SCP,and the amount of butyric acid was significantly improved.Consequently,the aforementioned results suggest that SCP isolated from S.chinensis is a potent candidate for use as a new health functional food and pharmaceutical ingredient for colitis treatment.展开更多
基金the Project of National Natural Science Foundation of China(32202071)Zhejiang Basic Public Welfare Research Project(LQ22C200003)+1 种基金Zhejiang Province Key R&D Project(2021C02001)the Key Research and Development Program of Ningxia Province(2022BBF02006),China.
文摘Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharides.This study extracts pectic polysaccharides from goji berry,raspberry,and cranberry,examines how HPP influences the pectic polysaccharide structure of three berries,and provides a basis for the extraction and modification of pectic polysaccharides with specific structure and bioactivity.Materials and methods:An orthogonal test was performed to optimize the HPP-assisted alkali method to extract the high yield and high RG-I content pectic polysaccharides from three berries.Structural information of pectic polysaccharides extracted by the HPP method and conventional methods were compared from the perspectives of monosaccharide composition,molecular weight,Fourier transform infrared spectroscopy,andnuclearmagneticresonancespectroscopy.Results:For raspberry,the optimal conditions consisted of a pressure of 50o MPa,a pH of 13,and a pressure-holding time of 12 min,while the optimal conditions for goji berry and cranberry were both 40o MPa,pH 13,and 15 min.Under the optimal conditions,the yields for goji berry,raspberry,and cranberry were 10.49%,16.63%,and 17.52%,respectively,and RG-l contents were 81.85%,83.30%,and 63.22%,respectively.HPP showed an effect to degrade homogalacturonan backbones and side chains and increase the RG-I content to some extent.Conclusion:HPP-assisted alkali method was revealed to be an efficient method to extract high RG-I content pectic polysaccharides,especially for cranberry,and was a potential method to modify pectic polysaccharide structure in a certain way.
文摘AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management.
基金supported by the PharmacoBio Assistantship 2019supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(NRF-2020R1F1A1070996).
文摘In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude polysaccharides(SCP)were extracted using hot water extraction and ethanol precipitation.SCP is composed primarily of eight monosaccharides,including galacturonic acid(46.6%),galactose(10.4%),arabinose(5.6%),and rhamnose(3.8%),that are typical sugar components of pectic polysaccharides.Oral administration of SCP recovered body weight loss and significantly alleviated various ulcerative colitis symptoms such as stool consistency,blood in stool,and blood in the anus.Additionally,the DSS-treated group that exhibited a decrease in colon length,and SCP treatment significantly reduced colon length shortening in a dose-dependent manner.Levels of inflammatory factors such as interleukin-6,tumor necrosis factor-α,and myeloperoxidase were lower in the SCP-treated groups than they were in the DSS-treated group.In contrast,Alcian blue-periodic acid Schiff staining revealed that SCP treatment effectively maintained the amount of mucus and goblet cells in the intestinal lumen compared to that in the DSS-treated group.The short-chain fatty acid content decreased by DSS administration was recovered through oral administration of SCP,and the amount of butyric acid was significantly improved.Consequently,the aforementioned results suggest that SCP isolated from S.chinensis is a potent candidate for use as a new health functional food and pharmaceutical ingredient for colitis treatment.
