Rhizobium tropici-derived extracellular polymeric substances (EPS) have been used in soils to enhance soil structures and mitigate soil erosions. However, information on their use to improve soil health and fertility ...Rhizobium tropici-derived extracellular polymeric substances (EPS) have been used in soils to enhance soil structures and mitigate soil erosions. However, information on their use to improve soil health and fertility indicators, and plant growth is limited. In a greenhouse study, we investigated their effects on some soil health, soil fertility indices, and the growth of black-eyed peas (Vigna unguiculate). Results showed that soils incubated with EPS significantly increased basal soil respiration, soil microbial biomass, permanganate oxidizable carbon (POC), and potentially mineralizable nitrogen (PMN). The EPS shifted microbial populations from bacteria to fungi and Gram (−ve) to Gram ( ve) bacteria. However, it had little or no effects on soil pH, soil organic matter (SOM), and cation exchange capacity (CEC). The EPS decreased soil moisture loss, increased soil aggregate stability, but delayed blacked-eyed peas germinations in the soils. At 0.1% (w/w) concentrations in soils, there was increase in plant root nodulations and vegetative growth. This study was carried out within 40 days of incubating soils with EPS or growing the black-eyed peas in a greenhouse study. The plant growth parameters were taken before flowering and fruiting. Further studies of the effects of incubating soils with the extracellular polymeric substances on plant growth. Soil microbial biomass, microbial diversities, and other soil fertility indices are deemed necessary.展开更多
The cultivation and management of different legume species and their varieties were tested in South-Western Finland at farmers＇ fields in two years, 2010 and 2011. The studied legumes were peas and lava beans, and in...The cultivation and management of different legume species and their varieties were tested in South-Western Finland at farmers＇ fields in two years, 2010 and 2011. The studied legumes were peas and lava beans, and included also such species which are seldom or not normally cultivated in Finland, namely soybeans and blue and white lupins. Seed samples were collected from legume farms. The quality of different legume species was analysed and compared to each others. The chemical qualities were analysed for crude protein, fat, fibre, ash, nitrogen-free extract and phytic acid contents, and amino acid composition. The quality of the varieties of lupins and soybeans were compared to the quality of pea and fava bean varieties, which are generally cultivated nowadays in Finland. The highest protein contents were in soybean （369 and 379 g kg^-1） and white lupin （382 g kg^-1） and the lowest in pea varieties. Amino acid composition of legumes is good, but lupins have lower lysine content （g 100 g^-1 protein） than other legumes. High fat contents were in soybeans （91 and 100 g kg^-1） and white lupin （101 g kgl）, and moderate in blue lupin （30-49 g kg^-1）. Fuego fava bean variety had lower protein content than Kontu. The highest phytic acid contents were in soybeans （18.9 and 22.6 mg kg^-1） and the lowest in white lupin （6.2 mg kg^-1）. Lupins are interesting protein crops, especially white lupin with high protein and fat and low phytic acid contents. Lupins are not day length sensitive, like soybean, and therefore valuable for long-day cultivation conditions The present research strengthens the good chemical quality of legumes for food and feed purposes.展开更多
The present research work was carried out to investigate the proximate composition and antioxidant capacities of newly invented varieties of dry peas. The proximate composition in terms of moisture （7.00-8.50%）, ash...The present research work was carried out to investigate the proximate composition and antioxidant capacities of newly invented varieties of dry peas. The proximate composition in terms of moisture （7.00-8.50%）, ash （3.85-4.97%）, fiber （3.8-12.3%） and protein （17.3-18.8%） contents varied significantly with respect to various cultivars. Total phenolic contents （TPC）, total flavonoids contents （TFC）, percentage inhibition of peroxidation of linoleic acid system, reducing power and DPPH free radical scavenging capacity of different dry peas extracts were found in range of 0.651-0.684 g/100g of GAE, 0.021-0.041 g/100g of CE, 0.713-0.895, 40.051-84.608%, 18.097-24.591 mg/mL, respectively. The amount of TFC, and percent inhibition of per oxidation in linoleic system of different dry peas extracts varied significantly among various cultivars and solvents. From the results of present investigations it is reasonable to say that 80% methanolic extracts of dry peas have exhibited varying degree of antioxidant activity.展开更多
This study was conducted at Fafan Research Center, Golajo research site to evaluate the effect of Moringa stenopetala and pigeon pea leaf supplementation on growth performance of short-eared Somali goat breed. A total...This study was conducted at Fafan Research Center, Golajo research site to evaluate the effect of Moringa stenopetala and pigeon pea leaf supplementation on growth performance of short-eared Somali goat breed. A total of fifteen yearling indigenous short-eared Somali goat breeds with an initial weight of 15.2 ± 0.30 kg were assigned to three treatment groups using completely randomized design. Pigeon pea (Pp) and Moringa stenopetala (MS) feeds were formulated using 0%, 5%, and 10% inclusion levels of MSLM and PPLM as experimental diets, respectively. The feed of the experiment was prepared in two levels (2 kg of Moringa stenopetala and 2 kg of pigeon pea) and was supplemented to experimental animals in treatments one and two, respectively. The average e initial body weight of selected male goats was 18.82 ± 0.37, 18.8 ± 0.37 and 17.8 ± 0.37 kg under treatment groups T1, T2 and control respectively. Data was analyzed using general linear model (GLM) procedure of SAS computer package Version 9.0 (SAS, 2002). The final weights gain (FWG) of goats on T1 and T2 of experimental group was significantly (P 0.05) the final weight gain of goat supplemented on Moringa stenopetala (T1) and pigeon pea levels (T2). The mean average weight gains (AWG) obtained from the supplemented group in this study were 7.50 ± 0.37 and 7.82 ± 0.37 kg for T1 and T2, whereas mean weight gains for un-supplemented goats were found to be 6.26 ± 0.37 kg. Feeding of dried Moringa stenopetala and pigeon pea leaves mixture improved body weights and average daily body weight gain without affecting feed intake and overall health of Somali goat breed. As Moringa stenopetala and pigeon pea leaves are rich nitrogen/protein source, they can be used effectively as substitute for conventional concentrate in the diet of growing goats at small holder farmer’s level where they can be grown in abundance. Therefore, for higher quality of forage and higher total DM yield for animal feeding, moringa should be harvested at wider harvesting intervals of at least 6th- to 8th-week intervals. Similarly, for pigeon peas, 4- to 6-week harvesting interval can result in optimum forage as well as feed quality and resulted in better growth performances for Somali short-eared goat breeds.展开更多
This paper attempts to analyze the kinetics involved in the drying of green peas in‘with blanching’and‘without blanching’techniques.Blanching by hot water mixed with a solution of citric acid(0.1–0.2 mg/ml)at 70...This paper attempts to analyze the kinetics involved in the drying of green peas in‘with blanching’and‘without blanching’techniques.Blanching by hot water mixed with a solution of citric acid(0.1–0.2 mg/ml)at 70C–100C is the treatment provided to the samples of green peas.Experimental analysis shows that the moisture content in green peas of three different sizes is reduced at different temperatures and demonstrates the effect of the rate of drying of the moisture content.Under different diameters and temperatures,the parameters are analyzed using‘with blanching’and‘without blanching’techniques.It is observed that‘with blanching’process plays a significant role in the reduction of moisture content under different temperatures and diameters in a lesser time as compared to‘without blanching’.The operative activation energy and moisture diffusivity are described by using Fick’s law of diffusion.The calculation of effective moisture diffusivity can be done via the utilization of slope.The drying data is subjected to two models of mathematical nature:Simple Exponential model,and Page model.The performance of these models is examined by means of comparing the coefficient of determination(R2),chi-square(v2)and root mean square error(RMSE)between the experimental and forecasted value of moisture ratio obtained‘with blanching’and‘without blanching’.The experimental data was seen to be in accordance with the Page model.The comparisons of energy consumption,energy efficiency and also the cost of the drying processes for‘with blanching’and‘without blanching’have been accomplished to optimize and reduce process condition and the cost of the process,respectively.展开更多
Pigeon peas, a type of pulse, have immense nutritional potential to improve health in arid and semi-arid regions. However, unlocking this potential relies heavily on understanding their technological properties, such ...Pigeon peas, a type of pulse, have immense nutritional potential to improve health in arid and semi-arid regions. However, unlocking this potential relies heavily on understanding their technological properties, such as hydration rate, volumetric expansion, and cooking time. These properties directly influence processing, accessibility, and acceptability as a food source. However, there is limited information on technological properties of improved varieties. The study aimed to determine technological properties of improved pigeon pea varieties grown in Machakos County. Seven improved pigeon peas varieties namely: KARI Mbaazi 1, KARI Mbaazi 2, ICEAP 00850, KAT 60/8, Mituki, Egerton Mbaazi 1, Egerton Mbaazi 2 and ICEAP 00554 (control variety) were used in this study. These varieties were tested for water absorption capacity (WAC), volumetric expansion, density, cooking time (CT) and total soluble solids (TSS) in the broth. The experiment was arranged in a Completely Randomized Design (CRD) replicated three times. Data analysis was conducted using SAS software version 9.1.3 (SAS, 2006). Means separation was done using Tukey’s honestly significant difference (HSD) at 95% Confidence Level. There were significant differences in water absorption capacity (WAC), volumetric expansion, density, TSS, and CT among the improved varieties (p p < 0.05). KARI Mbaazi 2 exhibited the greatest volumetric expansion after cooking (VEAC) at 11%. Additionally, control variety recorded the highest water absorption capacity (125.48%), which was significantly greater compared to the improved pigeon pea varieties. Cooking time in minutes was shortest for Mituki (46.0) and KAT 60/8 (55.7) and longest for both KARI Mbaazi 1 and ICEAP00850 at 160 minutes. All the varieties showed high TSS ranging from 10.5 to 26.7% indicating the potential to select varieties with desired flavour profiles. Improved pigeon pea varieties (Mituki and KAT60/8) displayed desired technological properties alongside the control variety. These findings inform the specific culinary applications and nutritional needs which enhance utilisation of pigeon peas as food. Further research is needed to determine the impact of the technological properties on the digestibility and glycaemic index of pigeon peas.展开更多
BACKGROUND Cholangiocarcinoma(CCA)is a lethal malignancy with limited treatment options and poor prognosis.The PEA3 subfamily of E26 transformation specific genes:ETV1,ETV4,and ETV5 are known to play significant roles...BACKGROUND Cholangiocarcinoma(CCA)is a lethal malignancy with limited treatment options and poor prognosis.The PEA3 subfamily of E26 transformation specific genes:ETV1,ETV4,and ETV5 are known to play significant roles in various cancers by influencing cell proliferation,invasion,and metastasis.AIM To analyze PEA3 subfamily gene expression levels in CCA and their correlation with clinical parameters to determine their prognostic value for CCA.METHODS The expression levels of PEA3 subfamily genes in pan-cancer and CCA data in the cancer genome atlas and genotype-tissue expression project databases were analyzed with R language software.Survival curve and receiver operating characteristic analyses were performed using the SurvMiner,Survival,and Procr language packages.The gene expression profiling interactive analysis 2.0 database was used to analyze the expression levels of PEA3 subfamily genes in different subtypes and stages of CCA.Web Gestalt was used to perform the gene ontology/Kyoto encyclopedia of genes and genomes(GO/KEGG)analysis,and STRING database analysis was used to determine the genes and proteins related to PEA3 subfamily genes.RESULTS ETV1,ETV4,and ETV5 expression levels were significantly increased in CCA.There were significant differences in ETV1,ETV4,and ETV5 expression levels among the different subtypes of CCA,and predictive analysis revealed that only high ETV1 and ETV4 expression levels were significantly associated with shorter overall survival in patients with CCA.GO/KEGG analysis revealed that PEA3 subfamily genes were closely related to transcriptional misregulation in cancer.In vitro and in vivo experiments revealed that PEA3 silencing inhibited the invasion and metastasis of CCA cells.CONCLUSION The expression level of ETV4 may be a predictive biomarker of survival in patients with CCA.展开更多
Cholangiocarcinoma(CCA),a highly aggressive bile duct cancer,is associated with late-stage diagnosis and limited treatment options,leading to poor patient outcomes.Early detection and personalized treatment strategies...Cholangiocarcinoma(CCA),a highly aggressive bile duct cancer,is associated with late-stage diagnosis and limited treatment options,leading to poor patient outcomes.Early detection and personalized treatment strategies are crucial.The study by Wang et al highlights the prognostic potential of the PEA3 subfamily genes(ETV1,ETV4,and ETV5)in CCA,identifying ETV4 as a particularly promising biomarker.Their bioinformatic analysis revealed that elevated ETV4 expression correlates with poorer survival,positioning it as a strong indicator of disease progression.These findings suggest that ETV4 could enhance prognostic precision and guide personalized therapies,although further validation through large-scale clinical trials is essential.Challenges in clinical application include the need for comprehensive experimental validation and addressing the tumor heterogeneity in CCA.Future research should focus on validating these biomarkers in diverse cohorts and developing targeted therapies,especially in regions where CCA is endemic.展开更多
Grass pea(Lathyrus sativus L.)is an imperative food crop cultured in dryland agricultural ecology.It is a vital source of dietary protein to millions of populaces living in low-income countries in South-East Asia and ...Grass pea(Lathyrus sativus L.)is an imperative food crop cultured in dryland agricultural ecology.It is a vital source of dietary protein to millions of populaces living in low-income countries in South-East Asia and Africa.This study highlights the improvement of genomic properties and their application in marker-trait relationships for 17 yield-related characters in 400 grass pea genotypes from China and Bangladesh.These characters were assessed via 56 polymorphic markers using general linear model(GLM)(P+G+Q)and mixed linear model(MLM)(P+G+Q+K)in the tassel software based on the linkage disequilibrium and population structure analysis.Population structure analysis showed two major groups and one admixed group in the populace.Statistically significant loci pairs of linkage disequilibrium(LD)mean value(D′)was 0.479.A total of 99 and 61 marker-trait associations in GLM and MLM models allied to the 17 traits were accepted at a 5%level of significance.Among these markers,21 markers were associated with more than one trait;12 marker-trait associations passed the Bonferroni correction threshold.Both models found six markers C41936,C39067,C34100,C47146,C47638,and C43047 significantly associated with days to maturity,flower color,plant height,and seed per pod were detected in the Hebei and Liaoyang location(p≤0.01),and the interpretation rate(R^(2)value)11.2%to 43.6%.Conferring to the consequences,the association analysis methodology may operative system for quantitative,qualitative,and biochemical traits related to gene position mapping and support breeders in improving novel approaches for advancing the grass pea quality.展开更多
Chronic thromboembolic pulmonary hypertension(CTEPH)is a chronic,progressive,debilitating,and life-threa-tening complication of pulmonary embolism(PE).Recent technological advances have permitted various treat-ment op...Chronic thromboembolic pulmonary hypertension(CTEPH)is a chronic,progressive,debilitating,and life-threa-tening complication of pulmonary embolism(PE).Recent technological advances have permitted various treat-ment options for the treatment of CTEPH,including surgery,angioplasty,and medical treatment,depending on the location and characteristics of lesions.Pulmonary endarterectomy(PEA)is the treatment of choice for CTEPH,as it offers excellent long-term outcomes and a high probability of recovery.Moreover,various medical and interventional therapies are currently being developed for patients with inoperable CTEPH.This review mainly summarizes the current treatment approaches of CTEPH,offering more options for specialist physicians to,thus,better manage chronic thromboembolic syndromes.展开更多
针对无线传感器网络探测网络环境的自适应休眠算法(Probing Environment and Adaptive Sleeping,PEAS)在节点调度过程中,存在节点能耗不均衡、网络的生命周期较短的问题,提出一种基于加权的优化覆盖算法。该算法对最小频繁项的目标所对...针对无线传感器网络探测网络环境的自适应休眠算法(Probing Environment and Adaptive Sleeping,PEAS)在节点调度过程中,存在节点能耗不均衡、网络的生命周期较短的问题,提出一种基于加权的优化覆盖算法。该算法对最小频繁项的目标所对应的传感节点按能量高低进行划分集合,使各集合能够独立覆盖最小频繁项的目标,以达到局部的优化。考虑到传感节点覆盖目标数和剩余能量对无线传感网络生存周期的影响,对边缘未覆盖的目标节点采用加权的方式进行覆盖。仿真结果表明:该算法能够均衡网络节点的能耗,有效地延长了网络的生命周期。展开更多
文摘Rhizobium tropici-derived extracellular polymeric substances (EPS) have been used in soils to enhance soil structures and mitigate soil erosions. However, information on their use to improve soil health and fertility indicators, and plant growth is limited. In a greenhouse study, we investigated their effects on some soil health, soil fertility indices, and the growth of black-eyed peas (Vigna unguiculate). Results showed that soils incubated with EPS significantly increased basal soil respiration, soil microbial biomass, permanganate oxidizable carbon (POC), and potentially mineralizable nitrogen (PMN). The EPS shifted microbial populations from bacteria to fungi and Gram (−ve) to Gram ( ve) bacteria. However, it had little or no effects on soil pH, soil organic matter (SOM), and cation exchange capacity (CEC). The EPS decreased soil moisture loss, increased soil aggregate stability, but delayed blacked-eyed peas germinations in the soils. At 0.1% (w/w) concentrations in soils, there was increase in plant root nodulations and vegetative growth. This study was carried out within 40 days of incubating soils with EPS or growing the black-eyed peas in a greenhouse study. The plant growth parameters were taken before flowering and fruiting. Further studies of the effects of incubating soils with the extracellular polymeric substances on plant growth. Soil microbial biomass, microbial diversities, and other soil fertility indices are deemed necessary.
文摘The cultivation and management of different legume species and their varieties were tested in South-Western Finland at farmers＇ fields in two years, 2010 and 2011. The studied legumes were peas and lava beans, and included also such species which are seldom or not normally cultivated in Finland, namely soybeans and blue and white lupins. Seed samples were collected from legume farms. The quality of different legume species was analysed and compared to each others. The chemical qualities were analysed for crude protein, fat, fibre, ash, nitrogen-free extract and phytic acid contents, and amino acid composition. The quality of the varieties of lupins and soybeans were compared to the quality of pea and fava bean varieties, which are generally cultivated nowadays in Finland. The highest protein contents were in soybean （369 and 379 g kg^-1） and white lupin （382 g kg^-1） and the lowest in pea varieties. Amino acid composition of legumes is good, but lupins have lower lysine content （g 100 g^-1 protein） than other legumes. High fat contents were in soybeans （91 and 100 g kg^-1） and white lupin （101 g kgl）, and moderate in blue lupin （30-49 g kg^-1）. Fuego fava bean variety had lower protein content than Kontu. The highest phytic acid contents were in soybeans （18.9 and 22.6 mg kg^-1） and the lowest in white lupin （6.2 mg kg^-1）. Lupins are interesting protein crops, especially white lupin with high protein and fat and low phytic acid contents. Lupins are not day length sensitive, like soybean, and therefore valuable for long-day cultivation conditions The present research strengthens the good chemical quality of legumes for food and feed purposes.
文摘The present research work was carried out to investigate the proximate composition and antioxidant capacities of newly invented varieties of dry peas. The proximate composition in terms of moisture （7.00-8.50%）, ash （3.85-4.97%）, fiber （3.8-12.3%） and protein （17.3-18.8%） contents varied significantly with respect to various cultivars. Total phenolic contents （TPC）, total flavonoids contents （TFC）, percentage inhibition of peroxidation of linoleic acid system, reducing power and DPPH free radical scavenging capacity of different dry peas extracts were found in range of 0.651-0.684 g/100g of GAE, 0.021-0.041 g/100g of CE, 0.713-0.895, 40.051-84.608%, 18.097-24.591 mg/mL, respectively. The amount of TFC, and percent inhibition of per oxidation in linoleic system of different dry peas extracts varied significantly among various cultivars and solvents. From the results of present investigations it is reasonable to say that 80% methanolic extracts of dry peas have exhibited varying degree of antioxidant activity.
文摘This study was conducted at Fafan Research Center, Golajo research site to evaluate the effect of Moringa stenopetala and pigeon pea leaf supplementation on growth performance of short-eared Somali goat breed. A total of fifteen yearling indigenous short-eared Somali goat breeds with an initial weight of 15.2 ± 0.30 kg were assigned to three treatment groups using completely randomized design. Pigeon pea (Pp) and Moringa stenopetala (MS) feeds were formulated using 0%, 5%, and 10% inclusion levels of MSLM and PPLM as experimental diets, respectively. The feed of the experiment was prepared in two levels (2 kg of Moringa stenopetala and 2 kg of pigeon pea) and was supplemented to experimental animals in treatments one and two, respectively. The average e initial body weight of selected male goats was 18.82 ± 0.37, 18.8 ± 0.37 and 17.8 ± 0.37 kg under treatment groups T1, T2 and control respectively. Data was analyzed using general linear model (GLM) procedure of SAS computer package Version 9.0 (SAS, 2002). The final weights gain (FWG) of goats on T1 and T2 of experimental group was significantly (P 0.05) the final weight gain of goat supplemented on Moringa stenopetala (T1) and pigeon pea levels (T2). The mean average weight gains (AWG) obtained from the supplemented group in this study were 7.50 ± 0.37 and 7.82 ± 0.37 kg for T1 and T2, whereas mean weight gains for un-supplemented goats were found to be 6.26 ± 0.37 kg. Feeding of dried Moringa stenopetala and pigeon pea leaves mixture improved body weights and average daily body weight gain without affecting feed intake and overall health of Somali goat breed. As Moringa stenopetala and pigeon pea leaves are rich nitrogen/protein source, they can be used effectively as substitute for conventional concentrate in the diet of growing goats at small holder farmer’s level where they can be grown in abundance. Therefore, for higher quality of forage and higher total DM yield for animal feeding, moringa should be harvested at wider harvesting intervals of at least 6th- to 8th-week intervals. Similarly, for pigeon peas, 4- to 6-week harvesting interval can result in optimum forage as well as feed quality and resulted in better growth performances for Somali short-eared goat breeds.
文摘This paper attempts to analyze the kinetics involved in the drying of green peas in‘with blanching’and‘without blanching’techniques.Blanching by hot water mixed with a solution of citric acid(0.1–0.2 mg/ml)at 70C–100C is the treatment provided to the samples of green peas.Experimental analysis shows that the moisture content in green peas of three different sizes is reduced at different temperatures and demonstrates the effect of the rate of drying of the moisture content.Under different diameters and temperatures,the parameters are analyzed using‘with blanching’and‘without blanching’techniques.It is observed that‘with blanching’process plays a significant role in the reduction of moisture content under different temperatures and diameters in a lesser time as compared to‘without blanching’.The operative activation energy and moisture diffusivity are described by using Fick’s law of diffusion.The calculation of effective moisture diffusivity can be done via the utilization of slope.The drying data is subjected to two models of mathematical nature:Simple Exponential model,and Page model.The performance of these models is examined by means of comparing the coefficient of determination(R2),chi-square(v2)and root mean square error(RMSE)between the experimental and forecasted value of moisture ratio obtained‘with blanching’and‘without blanching’.The experimental data was seen to be in accordance with the Page model.The comparisons of energy consumption,energy efficiency and also the cost of the drying processes for‘with blanching’and‘without blanching’have been accomplished to optimize and reduce process condition and the cost of the process,respectively.
文摘Pigeon peas, a type of pulse, have immense nutritional potential to improve health in arid and semi-arid regions. However, unlocking this potential relies heavily on understanding their technological properties, such as hydration rate, volumetric expansion, and cooking time. These properties directly influence processing, accessibility, and acceptability as a food source. However, there is limited information on technological properties of improved varieties. The study aimed to determine technological properties of improved pigeon pea varieties grown in Machakos County. Seven improved pigeon peas varieties namely: KARI Mbaazi 1, KARI Mbaazi 2, ICEAP 00850, KAT 60/8, Mituki, Egerton Mbaazi 1, Egerton Mbaazi 2 and ICEAP 00554 (control variety) were used in this study. These varieties were tested for water absorption capacity (WAC), volumetric expansion, density, cooking time (CT) and total soluble solids (TSS) in the broth. The experiment was arranged in a Completely Randomized Design (CRD) replicated three times. Data analysis was conducted using SAS software version 9.1.3 (SAS, 2006). Means separation was done using Tukey’s honestly significant difference (HSD) at 95% Confidence Level. There were significant differences in water absorption capacity (WAC), volumetric expansion, density, TSS, and CT among the improved varieties (p p < 0.05). KARI Mbaazi 2 exhibited the greatest volumetric expansion after cooking (VEAC) at 11%. Additionally, control variety recorded the highest water absorption capacity (125.48%), which was significantly greater compared to the improved pigeon pea varieties. Cooking time in minutes was shortest for Mituki (46.0) and KAT 60/8 (55.7) and longest for both KARI Mbaazi 1 and ICEAP00850 at 160 minutes. All the varieties showed high TSS ranging from 10.5 to 26.7% indicating the potential to select varieties with desired flavour profiles. Improved pigeon pea varieties (Mituki and KAT60/8) displayed desired technological properties alongside the control variety. These findings inform the specific culinary applications and nutritional needs which enhance utilisation of pigeon peas as food. Further research is needed to determine the impact of the technological properties on the digestibility and glycaemic index of pigeon peas.
基金Science and Technology Development Plan Project of Hangzhou,No.20201203B56.
文摘BACKGROUND Cholangiocarcinoma(CCA)is a lethal malignancy with limited treatment options and poor prognosis.The PEA3 subfamily of E26 transformation specific genes:ETV1,ETV4,and ETV5 are known to play significant roles in various cancers by influencing cell proliferation,invasion,and metastasis.AIM To analyze PEA3 subfamily gene expression levels in CCA and their correlation with clinical parameters to determine their prognostic value for CCA.METHODS The expression levels of PEA3 subfamily genes in pan-cancer and CCA data in the cancer genome atlas and genotype-tissue expression project databases were analyzed with R language software.Survival curve and receiver operating characteristic analyses were performed using the SurvMiner,Survival,and Procr language packages.The gene expression profiling interactive analysis 2.0 database was used to analyze the expression levels of PEA3 subfamily genes in different subtypes and stages of CCA.Web Gestalt was used to perform the gene ontology/Kyoto encyclopedia of genes and genomes(GO/KEGG)analysis,and STRING database analysis was used to determine the genes and proteins related to PEA3 subfamily genes.RESULTS ETV1,ETV4,and ETV5 expression levels were significantly increased in CCA.There were significant differences in ETV1,ETV4,and ETV5 expression levels among the different subtypes of CCA,and predictive analysis revealed that only high ETV1 and ETV4 expression levels were significantly associated with shorter overall survival in patients with CCA.GO/KEGG analysis revealed that PEA3 subfamily genes were closely related to transcriptional misregulation in cancer.In vitro and in vivo experiments revealed that PEA3 silencing inhibited the invasion and metastasis of CCA cells.CONCLUSION The expression level of ETV4 may be a predictive biomarker of survival in patients with CCA.
文摘Cholangiocarcinoma(CCA),a highly aggressive bile duct cancer,is associated with late-stage diagnosis and limited treatment options,leading to poor patient outcomes.Early detection and personalized treatment strategies are crucial.The study by Wang et al highlights the prognostic potential of the PEA3 subfamily genes(ETV1,ETV4,and ETV5)in CCA,identifying ETV4 as a particularly promising biomarker.Their bioinformatic analysis revealed that elevated ETV4 expression correlates with poorer survival,positioning it as a strong indicator of disease progression.These findings suggest that ETV4 could enhance prognostic precision and guide personalized therapies,although further validation through large-scale clinical trials is essential.Challenges in clinical application include the need for comprehensive experimental validation and addressing the tumor heterogeneity in CCA.Future research should focus on validating these biomarkers in diverse cohorts and developing targeted therapies,especially in regions where CCA is endemic.
基金the financial support from the Protection and Utilization of Crop Germplasm Resources project from the Ministry of Agriculture and Rural Affairs of China(2019NWB036-07)China Agriculture Research System of MOF and MARA-Food Legumes(CARS-08)+2 种基金National Infrastructure for Crop Germplasm Resources Project from the Ministry of Science and Technology of China(NICGR2019)Agricultural Science and Technology Innovation Program(ASTIP)in CAAS and Bangladesh-Second Phase of the National Agricultural Technology Program-Phase II Project,Bangladesh Agricultural Research Council(BARC),Bangladesh(P149553)supported by Researchers Supporting Project Number(RSP2025R7),King Saud University,Riyadh,Saudi Arabia.
文摘Grass pea(Lathyrus sativus L.)is an imperative food crop cultured in dryland agricultural ecology.It is a vital source of dietary protein to millions of populaces living in low-income countries in South-East Asia and Africa.This study highlights the improvement of genomic properties and their application in marker-trait relationships for 17 yield-related characters in 400 grass pea genotypes from China and Bangladesh.These characters were assessed via 56 polymorphic markers using general linear model(GLM)(P+G+Q)and mixed linear model(MLM)(P+G+Q+K)in the tassel software based on the linkage disequilibrium and population structure analysis.Population structure analysis showed two major groups and one admixed group in the populace.Statistically significant loci pairs of linkage disequilibrium(LD)mean value(D′)was 0.479.A total of 99 and 61 marker-trait associations in GLM and MLM models allied to the 17 traits were accepted at a 5%level of significance.Among these markers,21 markers were associated with more than one trait;12 marker-trait associations passed the Bonferroni correction threshold.Both models found six markers C41936,C39067,C34100,C47146,C47638,and C43047 significantly associated with days to maturity,flower color,plant height,and seed per pod were detected in the Hebei and Liaoyang location(p≤0.01),and the interpretation rate(R^(2)value)11.2%to 43.6%.Conferring to the consequences,the association analysis methodology may operative system for quantitative,qualitative,and biochemical traits related to gene position mapping and support breeders in improving novel approaches for advancing the grass pea quality.
文摘Chronic thromboembolic pulmonary hypertension(CTEPH)is a chronic,progressive,debilitating,and life-threa-tening complication of pulmonary embolism(PE).Recent technological advances have permitted various treat-ment options for the treatment of CTEPH,including surgery,angioplasty,and medical treatment,depending on the location and characteristics of lesions.Pulmonary endarterectomy(PEA)is the treatment of choice for CTEPH,as it offers excellent long-term outcomes and a high probability of recovery.Moreover,various medical and interventional therapies are currently being developed for patients with inoperable CTEPH.This review mainly summarizes the current treatment approaches of CTEPH,offering more options for specialist physicians to,thus,better manage chronic thromboembolic syndromes.
文摘针对无线传感器网络探测网络环境的自适应休眠算法(Probing Environment and Adaptive Sleeping,PEAS)在节点调度过程中,存在节点能耗不均衡、网络的生命周期较短的问题,提出一种基于加权的优化覆盖算法。该算法对最小频繁项的目标所对应的传感节点按能量高低进行划分集合,使各集合能够独立覆盖最小频繁项的目标,以达到局部的优化。考虑到传感节点覆盖目标数和剩余能量对无线传感网络生存周期的影响,对边缘未覆盖的目标节点采用加权的方式进行覆盖。仿真结果表明:该算法能够均衡网络节点的能耗,有效地延长了网络的生命周期。
