[Objective] The paper was to select different fungicides for field efficacy test against pear scab in summer.[Method] Five treatments were designed in the test,including 10% difenoconazole WDG 6 000 times dilution,50%...[Objective] The paper was to select different fungicides for field efficacy test against pear scab in summer.[Method] Five treatments were designed in the test,including 10% difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole 4 000 times dilution,and water(control).[Result] 10% Difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole SC 4 000 times dilution had good control effects against pear scab,and the efficacies were 89.13%-93.51%.[Conclusion] The results will provide the theoretical basis for field control of pear scab in summer.展开更多
以‘鸭梨’(Pyrusbretschneideri)ב雪青’梨(P.bretschneideri×P.pyrifolia)F1代群体(97株)为试材,采用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术和集群分类法(bulked segregantanalysis,BSA)...以‘鸭梨’(Pyrusbretschneideri)ב雪青’梨(P.bretschneideri×P.pyrifolia)F1代群体(97株)为试材,采用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术和集群分类法(bulked segregantanalysis,BSA)筛选与梨抗黑星病基因连锁的分子标记。通过64对AFLP标记引物在亲本和分离群体中的筛选和验证,获得与梨抗黑星病基因紧密连锁标记两个,即ACA/CAA-179和AAC/CAG-198。它们与抗黑星病基因的遗传距离分别为5.2和8.3cM。对AFLP标记片段的克隆和测序结果显示其长度分别为179和198bp。根据序列信息设计特异引物,在杂交后代群体上的PCR分析表明AFLPACA/CAA-179标记被成功转换成SCAR标记,命名为SCAR-117。本研究结果将有助于对抗黑星病梨品种资源的分子鉴定及杂种后代的早期辅助选择。展开更多
Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population deri...Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.展开更多
为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病...为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病菌后的抗性调查,并进行了AFLP分子标记的研究。结果表明,棚内接种梨黑星病菌后,杂交后代抗感分离规律符合双假测交的遗传规律;研究建立的AFLP分子标记体系可以用于梨黑星病的抗性基因标记,并初步找到了与梨黑星病抗性基因相关的分子标记。展开更多
基金Supported by Youth Fund Project of Hebei Academy of Agriculture and Forestry Sciences(2018020101)Science and Technology Plan of Hebei Province (16246327D)Special Financial Project of Hebei Province (F18R1908,2019-3-5-2)。
文摘[Objective] The paper was to select different fungicides for field efficacy test against pear scab in summer.[Method] Five treatments were designed in the test,including 10% difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole 4 000 times dilution,and water(control).[Result] 10% Difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole SC 4 000 times dilution had good control effects against pear scab,and the efficacies were 89.13%-93.51%.[Conclusion] The results will provide the theoretical basis for field control of pear scab in summer.
文摘以‘鸭梨’(Pyrusbretschneideri)ב雪青’梨(P.bretschneideri×P.pyrifolia)F1代群体(97株)为试材,采用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术和集群分类法(bulked segregantanalysis,BSA)筛选与梨抗黑星病基因连锁的分子标记。通过64对AFLP标记引物在亲本和分离群体中的筛选和验证,获得与梨抗黑星病基因紧密连锁标记两个,即ACA/CAA-179和AAC/CAG-198。它们与抗黑星病基因的遗传距离分别为5.2和8.3cM。对AFLP标记片段的克隆和测序结果显示其长度分别为179和198bp。根据序列信息设计特异引物,在杂交后代群体上的PCR分析表明AFLPACA/CAA-179标记被成功转换成SCAR标记,命名为SCAR-117。本研究结果将有助于对抗黑星病梨品种资源的分子鉴定及杂种后代的早期辅助选择。
文摘Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
文摘为了解梨黑星病抗性遗传规律并为梨黑星病抗性育种早期选择提供理论依据,以新苹梨(梨黑星病抗性品种)×雪花梨(梨黑星病易感品种)的杂交后代为试材,进行了田间自然感病和棚内接种梨黑星病(Venturia nashicolaTanak et Yamamota)病菌后的抗性调查,并进行了AFLP分子标记的研究。结果表明,棚内接种梨黑星病菌后,杂交后代抗感分离规律符合双假测交的遗传规律;研究建立的AFLP分子标记体系可以用于梨黑星病的抗性基因标记,并初步找到了与梨黑星病抗性基因相关的分子标记。
