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Identification of Two Botryosphaeria dothidea Strains Infecting Leaves of Yellow Peach ( Amygdalus persica )
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作者 Siqing YI Guangning PENG +6 位作者 Kaifa GUO Jianhong YAN Yanhong LEI Shiyu CAO Xianzhi NI Chenzhong JIN Hongyan LIU 《Agricultural Biotechnology》 2026年第1期47-49,53,共4页
[Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty lea... [Objectives]This study was conducted to clarify the species composition of pathogens causing leaf spot disease in yellow peach in Loudi area of Hunan Province and to screen effective control agents.[Methods]Twenty leaf spot samples were collected from yellow peach planting bases in Lengshuijiang and Shuangjiang,Loudi City.Two isolates were obtained through tissue isolation and purification.Identification was performed based on morphological characteristics combined with rDNA-ITS and Bt-2a gene sequence analysis and pathogenicity assay.[Results]The pathogen causing yellow peach leaf spot in this area was Botryosphaeria dothidea.[Conclusions]This study provides a reference basis for the prevention and control of yellow peach leaf spot in Loudi area. 展开更多
关键词 Loudi Yellow peach Leaf spot disease Botryosphaeria dothidea
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Peach Code Unlocked The story of the Jinshan flat peach exemplifies China’s efforts to support agricultural heritage protection
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作者 Wang Huiqiong 《China Report ASEAN》 2025年第10期38-40,共3页
In a peach orchard in Lyuxiang Town,Jinshan District,Shanghai,youth delegates from 13 countries,officials from the Food and Agriculture Organization of the United Nations(FAO),and agricultural heritage experts were im... In a peach orchard in Lyuxiang Town,Jinshan District,Shanghai,youth delegates from 13 countries,officials from the Food and Agriculture Organization of the United Nations(FAO),and agricultural heritage experts were impressed by sweet and juicy Jinshan flat peaches.Jinshan has a history of flat peach cultivation that spans hundreds of years dating back to the Yuan Dynasty(1271-1368). 展开更多
关键词 flat peaches flat peach agricultural heritage Shanghai youth delegates China Jinshan District agricultural heritage experts
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Genome-wide identification of the PpSWEET gene family and function characterization of PpSWEET6 associated with endodormancy release in peach bud
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作者 Sen Li Ge Tian +5 位作者 Xiling Fu Wei Xiao Xiude Chen Dongmei Li Qingjie Wang Ling Li 《Journal of Integrative Agriculture》 2025年第11期4255-4270,共16页
Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and si... Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation. 展开更多
关键词 peach bud endodormancy SWEET family PpSWEET6 PpABF2
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Peaches (Prunus persica L.) pulp mitigate type 2 diabetic mice by modulating of glucose metabolism and gut microbiota
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作者 Leyi Zhou Jianfen Ye +4 位作者 Yingying Wei Mouayche Salma Shu Jiang Yi Chen Xingfeng Shao 《Food Science and Human Wellness》 2025年第9期3681-3695,共15页
Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on t... Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes. 展开更多
关键词 peach pulp GLUCONEOGENESIS Glycogen synthesis Short-chain fatty acids Gut microbiota
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Facile Preparation of Robust Peach Gum Polysaccharide with Remarkably Enhanced Antibacterial and Antioxidant Performance
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作者 Mengting Huang Meiting Lu +2 位作者 Li Yang Jiwen Long Li Zhou 《Journal of Renewable Materials》 2025年第10期2077-2090,共14页
Peach gum polysaccharide(PGP),a readily available natural polysaccharide,boasts substantial potential across diverse applications,yet its practical utility is severely limited by its vulnerability to bacterial growth ... Peach gum polysaccharide(PGP),a readily available natural polysaccharide,boasts substantial potential across diverse applications,yet its practical utility is severely limited by its vulnerability to bacterial growth and limited antioxidant activity.Herein,we introduced a simple and effective method to enhance the antibacterial and antioxidant properties of PGP by conjugating it with salicylic acid(SA).Cytotoxicity evaluation results confirmed that the resulting PGP-SA retains the excellent biocompatibility of PGP.Notably,PGP-SA demonstrates outstanding antibacterial activity against both Gram-positive(S.aureus)and Gram-negative(E.coli)bacteria,outperforming non-modified PGP.Its antibacterial mechanism is hypothesized to stem from disrupting bacterial cell membranes and proteins,targeting structures vital to microbial survival.Beyond fighting bacteria,PGP-SA also delivers robust antioxidant activity,efficiently scavenging ABTS radicals.Harnessing these dual enhancements,PGP-SA proves highly effective as a fruit preservation coating.Most impressively,it extends the post-harvest shelf life of mangoes:under identical storage conditions,PGP-SA-coatedmangoes stay fresh for seven extra days compared to uncoated fruit.With its simple synthesis process and standout performance,this work not only overcomes PGP’s key limitations but also opens new avenues for its application in food preservation and related industries. 展开更多
关键词 peach gum polysaccharide salicylic acid antibacterial activity antioxidant activity food preservation
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Effects of Potassium Application Rates on Leaf Growth and Soil Nutrients in Greenhouse Peaches
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作者 Yu WANG Yonghong LI +5 位作者 Jie LI Qihang TIAN Zhendong TIAN Guojian LIU Ruifeng CHANG Zhaoyuan WANG 《Asian Agricultural Research》 2025年第12期27-30,共4页
[Objectives]To clarify the effects of varying potassium application rates on leaf growth and soil mineral nutrients of greenhouse peaches,and to identify the optimal potassium fertilizer dosage.[Methods]Using the gree... [Objectives]To clarify the effects of varying potassium application rates on leaf growth and soil mineral nutrients of greenhouse peaches,and to identify the optimal potassium fertilizer dosage.[Methods]Using the greenhouse peach superior line C26-7-17 as the test material and maintaining a fixed ratio of nitrogen and phosphorus fertilizers,five potassium fertilizer treatment groups were established.The application rates of potassium sulfate were 0,67,135,202,and 269 g/plant,respectively.The regulatory effects of varying potassium application rates were analyzed by measuring leaf area,chlorophyll content,and soil mineral element content.[Results]Applying nitrogen,phosphorus,and potassium fertilizers in specific proportions increased the leaf area of greenhouse peaches,enhanced chlorophyll content,and significantly improved the soil s nutritional status.Considering the overall optimization of soil nutrients,the recommended potassium fertilizer dosage under the experimental conditions was 202 g/plant.[Conclusions]This study offers theoretical insights that may enhance the quality and efficiency of greenhouse peaches,as well as inform nutrient management strategies. 展开更多
关键词 Greenhouse peach Potassium application rate Leaf growth Soil mineral element Fertilization recommendation
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Key Technology of Peach Industry Chain Development in Zunyi City
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作者 Xiong YAN Xiaoxi GUAN +4 位作者 Xiangchun ZHANG Jun CHENG Xingyong WANG Detao YU Wenzhao LI 《Asian Agricultural Research》 2025年第3期12-14,共3页
In order to promote the development of the characteristic industry of fragrant crisp peach in Honghuagang District of Zunyi City,Guizhou Province,and assist the implementation of the rural revitalization strategy,base... In order to promote the development of the characteristic industry of fragrant crisp peach in Honghuagang District of Zunyi City,Guizhou Province,and assist the implementation of the rural revitalization strategy,based on the actual situation of the local forest land,this paper studied the development of under-forest economy and the development of products with high added value.Through the combination of field investigation and laboratory analysis,the technology of interplanting soybean in peach orchard was discussed in depth,and the processing technology of high-quality preserved peach was developed.This paper is intended to expand and extend the industrial chain of fragrant and crisp peaches,and to promote the development of modern mountain characteristic and efficient agriculture. 展开更多
关键词 Fragrant crisp peach Industrial chain Interplanting soybean Preserved fruit processing
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Effects of Soybean Intercropping on Soil N,P and K Elements in High-quality Peach Orchards
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作者 Yinhong WANG Xiong YAN +6 位作者 Shilan LI Wenzhao LI Kai HUANG Dan LIU Pingfan WU Jun CHENG Detao YU 《Agricultural Biotechnology》 2025年第5期19-21,共3页
[Objectives]This study was conducted to investigate the impact of soybean intercropping on soil fertility in the planting areas of high-quality peach orchards in Shenxi Town,Zunyi City,Guizhou Province.[Methods]Soil s... [Objectives]This study was conducted to investigate the impact of soybean intercropping on soil fertility in the planting areas of high-quality peach orchards in Shenxi Town,Zunyi City,Guizhou Province.[Methods]Soil samples were collected during the seedling,growth,and maturity stages of soybeans from both non-intercropped soil(control check group,CK)and soybean-intercropped soil(Qiandou 11,experimental group,EK).Analysis was conducted on soil fertility properties,including alkali-hydrolyzable N,available P,and available K.[Results]The impact of soybean intercropping on soil physicochemical properties was primarily manifested as the increased levels of alkali-hydrolyzable N and available K and the decreased level of available P in the soil during the seedling,growing,and maturity stages.Overall,this approach optimized and enhanced soil fertility in the peach orchards,providing a viable pathway for soil improvement in peach orchards located in karst areas.[Conclusions]The findings of this study provide a scientific basis for the high-quality development of high-quality peach orchards. 展开更多
关键词 Soybean intercropping High-quality peach orchard Soil fertility
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Blossom-Peach Forest in Spring Enlivens THE DEEP VALLEYS IN NYINGCHI
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作者 Zhou Qingqing Zhang Jing(Photos) Huang Wenjuan(Translated) 《China's Tibet》 2025年第2期60-65,共6页
Peach blossoms,long celebrated as harbingers of spring in Chinese poetry for over 2,000 years,reach full bloom around March and April.In harmony with the advent of spring and the flowering season,the tradition of appr... Peach blossoms,long celebrated as harbingers of spring in Chinese poetry for over 2,000 years,reach full bloom around March and April.In harmony with the advent of spring and the flowering season,the tradition of appreciating blossoms has become a hallmark of the spring excursion season in China.Amidst the vast sea of peach blossoms,the Nyingchi Peach Blossom Festival stands out prominently,drawing thousands of tourists to Xizang during the season. 展开更多
关键词 Nyingchi peach blossomslong appreciating blossoms
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无人机作业参数对雾滴在桃树冠层沉积分布的影响
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作者 蒋三生 卜小东 +1 位作者 郭辉 蒋晓 《中国农机化学报》 北大核心 2026年第1期147-152,共6页
为优选出无人机针对桃树喷施作业的参数组合,以双旋翼农业无人机为作业平台,设置三因素三水平的正交试验,通过试验结果和极差分析研究作业高度、作业速度、喷施流量对雾滴沉积密度、均匀性和穿透性的影响规律和影响程度。结果表明:作业... 为优选出无人机针对桃树喷施作业的参数组合,以双旋翼农业无人机为作业平台,设置三因素三水平的正交试验,通过试验结果和极差分析研究作业高度、作业速度、喷施流量对雾滴沉积密度、均匀性和穿透性的影响规律和影响程度。结果表明:作业高度H为3.5 m、作业速度V为2.5 m/s、喷施流量T为72 L/hm^(2)时,雾滴的沉积密度最佳,平均为34.03个/cm^(2),同时具有较好的沉积均匀性和穿透性;桃树上层沉积密度的较优因素水平:H=3.5 m、V=2.5 m/s、T=108 L/hm^(2),沉积分布均匀性的较优因素水平:H=4.0 m、V=2.0 m/s、T=72 L/hm^(2);桃树中、下层沉积密度的较优因素水平:H=4.0 m、V=2.5 m/s、T=72 L/hm^(2),中层沉积分布均匀性的较优因素水平:H=4.0 m、V=1.5 m/s、T=72 L/hm^(2),下层沉积分布均匀性的较优因素水平:H=3.5 m、V=2.5 m/s、T=90 L/hm^(2);雾滴沉积穿透性的较优因素水平:H=3.5 m、V=2.0 m/s、T=108 L/hm^(2);影响雾滴沉积效果的主次顺序在树冠上、中、下3个高度层均呈现不同的结果。综合对比发现,作业高度对喷施雾滴的沉积密度和沉积均匀性影响最大,作业速度次之,喷施流量的影响最小;影响雾滴沉积密度穿透性大小的因素主次顺序为喷施流量、作业高度、作业速度。试验结果可为农业无人机在果树产业中的绿色飞防应用和发展提供理论依据。 展开更多
关键词 无人机 作业参数 雾滴 沉积分布 桃树
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基于广泛靶向代谢组学分析桃果体内代谢物对异常威克汉姆酵母的响应
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作者 李军 周娅丽 +3 位作者 陈夕飞 张晓云 赵利娜 张红印 《现代食品科技》 北大核心 2026年第1期53-60,共8页
该研究主要探究采后桃果实对异常威克汉姆酵母(Wickerhamomyces anomalus)处理后的响应机制,明确桃果实体内的代谢物种类变化,以期为异常威克汉姆酵母处理提高桃果实抗病机制提供理论依据。利用广泛靶向代谢组学技术,结合UPLC-MS/MS方法... 该研究主要探究采后桃果实对异常威克汉姆酵母(Wickerhamomyces anomalus)处理后的响应机制,明确桃果实体内的代谢物种类变化,以期为异常威克汉姆酵母处理提高桃果实抗病机制提供理论依据。利用广泛靶向代谢组学技术,结合UPLC-MS/MS方法,对W.anomalus处理组与对照组的差异代谢物进行定性和定量分析。通过应用正交偏最小二乘-判别分析(OPLS-DA),构建判别模型,并据此筛选出关键的差异代谢物。最后通过绘制聚类热图、KEGG富集分析、主成分分析和相关性分析等,确定异常威克汉姆酵母处理后桃果体内关键差异代谢物质的变化。研究结果显示:W.anomalus处理组共筛选出175个差异代谢物,具体表现为115种代谢物含量增加,60种含量减少。这些差异代谢物涵盖了萜类、黄酮类、酚酸类化合物、生物碱、木脂素以及香豆素等多种类型。与抑菌、抗氧化、抵御生物和非生物胁迫、促进植物生长、抗植物衰老等功能相关的萜类、黄酮类、酚酸类、生物碱类次生代谢物质的积累,提高了桃果实的抗病能力。这些结果为未来拮抗酵母制剂的开发提供了可用信息。 展开更多
关键词 桃果 生物防治 异常威克汉姆酵母 代谢组学 差异代谢物
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晚青蜜桃新品种选育及高效栽培技术
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作者 高静 李田 +3 位作者 肖龙 韩霞 郭文娟 李永红 《落叶果树》 2026年第1期69-71,共3页
晚青蜜桃属极晚熟品种,由青州蜜桃实生选育出。果实近圆形,缝合线两半边对称,平均单果重102g,最大161 g。果面鲜红色,着色面积90%。果肉白色,肉质细腻,汁液中多,可溶性固形物含量18.3%,果实去皮硬度10.9kg/cm^(2)。品质优,抗旱性强,抗... 晚青蜜桃属极晚熟品种,由青州蜜桃实生选育出。果实近圆形,缝合线两半边对称,平均单果重102g,最大161 g。果面鲜红色,着色面积90%。果肉白色,肉质细腻,汁液中多,可溶性固形物含量18.3%,果实去皮硬度10.9kg/cm^(2)。品质优,抗旱性强,抗晚霜危害。在山东潍坊果实成熟期为10月上旬。总结了晚青蜜桃高效栽培技术,包括栽植、土肥水管理、整形修剪、花果管理、病虫害防治。 展开更多
关键词 桃新品种 晚青蜜 极晚熟 栽培技术
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省力化技术在天水市桃园的创新应用与实践
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作者 蒲建霞 陈建军 王耀辉 《果树资源学报》 2026年第1期114-117,共4页
为突破天水市桃园传统栽培中品种单一、劳动力依赖度高、成本攀升等瓶颈,通过构建“良种+良法+良机”技术体系,筛选出7个适宜当地的桃优良品种,创新应用“宽行密植+行内覆盖+行间生草+Y字树形+长枝修剪”栽培模式,集成病虫害绿色防控、... 为突破天水市桃园传统栽培中品种单一、劳动力依赖度高、成本攀升等瓶颈,通过构建“良种+良法+良机”技术体系,筛选出7个适宜当地的桃优良品种,创新应用“宽行密植+行内覆盖+行间生草+Y字树形+长枝修剪”栽培模式,集成病虫害绿色防控、水肥一体化、小微农机具应用等技术。示范推广实践表明,该技术体系使桃树产量增产17.31%,商品果率提升11.5%,亩(667m^(2))管理成本节支1 424.9元,节本率49.29%,同时减少化学农药使用30%以上。该项技术为西北干旱半干旱地区桃产业转型升级提供了可复制范式,也为乡村振兴提供了技术支撑。 展开更多
关键词 天水市 省力化技术 节本增效
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论《温柔乡传奇》及评点之文本重述探析
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作者 杨绪容 袁悦 《湖北师范大学学报(哲学社会科学版)》 2026年第1期55-60,共6页
星堂主人的长篇传奇《温柔乡》取材自余怀短篇笔记小说《板桥杂记》,在体例、情节、人物设置及评点上有模仿孔尚任《桃花扇》的痕迹,但其并非是了无新意的蹈袭之作。在叙事结构上,《温柔乡》受到李渔叙事理论的影响,运用“立主脑、减头... 星堂主人的长篇传奇《温柔乡》取材自余怀短篇笔记小说《板桥杂记》,在体例、情节、人物设置及评点上有模仿孔尚任《桃花扇》的痕迹,但其并非是了无新意的蹈袭之作。在叙事结构上,《温柔乡》受到李渔叙事理论的影响,运用“立主脑、减头绪、密针线”三大叙事策略,增强了剧本的整体连贯性。此外,该本在人物刻画与叙事虚实处理上亦呈现出有别于《板桥杂记》和《桃花扇》的创新,从而提升了其艺术价值。 展开更多
关键词 《温柔乡传奇》 《板桥杂记》 《桃花扇》 文本重述
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晚熟优质抗寒桃新品种秋悦的选育
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作者 田启航 李杰 +6 位作者 田振东 刘国俭 王雨 王召元 常瑞丰 魏静 李永红 《果树学报》 北大核心 2026年第1期215-218,共4页
秋悦是以优质桃品种秋燕与高抗寒野生半栽培品种东合1号为亲本,杂交选育而成的晚熟、抗寒新品种。果实圆形,果顶圆平,缝合线浅,两侧对称,平均单果质量334 g。果实完全成熟时果皮底色呈黄白色,果面90%以上着红色,茸毛较短;果肉白色,硬溶... 秋悦是以优质桃品种秋燕与高抗寒野生半栽培品种东合1号为亲本,杂交选育而成的晚熟、抗寒新品种。果实圆形,果顶圆平,缝合线浅,两侧对称,平均单果质量334 g。果实完全成熟时果皮底色呈黄白色,果面90%以上着红色,茸毛较短;果肉白色,硬溶质,风味甜,汁液含量中等,可溶性固形物含量(w,后同)13.6%果实硬度13.2 kg·cm^(-2),黏核。蔷薇型花,花粉量大,自花结实。树势强健,树姿半开张。在唐山、秦皇岛地区,该品种于4月中旬盛花,9月中下旬果实成熟,果实发育期约150 d。抗寒性强,产量高且稳定,适宜在河北省秦皇岛市昌黎县、青龙县,唐山市滦南县及生态条件类似地区栽培。 展开更多
关键词 新品种 秋悦 抗寒
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水蜜桃片干燥过程中孔隙形成对其力学特性和质构的影响
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作者 张博 赵邯 +6 位作者 赖新竹 王君骁 李大婧 刘春菊 牛丽影 马艳弘 吕春茂 《食品科学技术学报》 北大核心 2026年第1期180-194,共15页
果蔬片干燥过程中会产生孔隙,孔隙在此过程中的变化影响着果蔬片的力学性质和质构。为明确水蜜桃片干燥过程中孔隙形成对其力学特性和质构的影响,探究了水蜜桃片真空冷冻干燥(freeze-drying,FD)和冻干-压差膨化联合干燥(freeze-drying c... 果蔬片干燥过程中会产生孔隙,孔隙在此过程中的变化影响着果蔬片的力学性质和质构。为明确水蜜桃片干燥过程中孔隙形成对其力学特性和质构的影响,探究了水蜜桃片真空冷冻干燥(freeze-drying,FD)和冻干-压差膨化联合干燥(freeze-drying combined expansion puffing drying,FP)过程中,水蜜桃片孔隙微观结构及质构参数的变化规律,分析了孔隙形成与水蜜桃力学性能和质构的关系。结果表明,FD过程中样品孔隙逐渐变大;糖浸渍可以明显改善孔隙结构,促进较小孔隙的形成。FP过程中,随着水分含量降低,未浸渍样品孔隙逐渐变大,干燥后孔隙破裂严重,产生较大孔隙。糖浸渍样品在FP过程中孔隙变化较小,脆片断裂现象得到改善。蔗糖浸渍可以增加水蜜桃片组织刚性、硬度,降低黏弹性;低聚木糖浸渍有利于增加松弛特性与弹性。相关性分析和主成分分析发现,样品孔隙度(三维孔隙参数)对其硬度影响较大;二维孔隙参数中孔隙周长对硬度、弹性模量的影响较大,孔隙圆度对弹性影响较大。研究表明,水蜜桃片干燥过程中形成的孔隙结构直接影响其力学特性和质构,糖浸渍联合FP可调控其孔隙结构、改善水蜜桃片质构。研究旨在为果蔬干燥制品的品质优化提供理论参考和技术支撑。 展开更多
关键词 水蜜桃片 孔隙 干燥 力学特性 质构
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PpC3H37参与ALA提高桃花期子房抗寒性的分子机制
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作者 陈政 王晓俊 +6 位作者 严娟 蔡志翔 张斌斌 许建兰 马瑞娟 俞明亮 沈志军 《园艺学报》 北大核心 2026年第1期41-55,共15页
前期研究发现,5–氨基乙酰丙酸(5-aminolevulinic acid,ALA)能够上调PpC3H37促进PpWRKY表达提升桃花抗寒性。以此为基础,进一步研究完善其提高抗寒性的分子机制,明确了50 mg·L-1ALA预处理能够提高低温下桃花抗氧化酶超氧化物歧化酶... 前期研究发现,5–氨基乙酰丙酸(5-aminolevulinic acid,ALA)能够上调PpC3H37促进PpWRKY表达提升桃花抗寒性。以此为基础,进一步研究完善其提高抗寒性的分子机制,明确了50 mg·L-1ALA预处理能够提高低温下桃花抗氧化酶超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(catalase,CAT)活性以及脯氨酸、可溶性糖和可溶性蛋白含量,降低丙二醛和活性氧物质含量,从而增强桃花抗寒性。以PpC3H37启动子为诱饵进行酵母单杂交筛库,获得响应ALA上调表达转录因子PpC2H2。GUS染色和双荧光素酶实验表明ALA能够上调PpC2H2表达,增加PpC3H37启动子活性。瞬时过表达PpC2H2能够显著提升桃花器官抗寒性。 展开更多
关键词 花器官 ALA 低温胁迫 抗寒性 PpC3H37 PpC2H2
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枯草芽孢杆菌J6-1对桃褐腐病菌的抑菌活性分析
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作者 李晓倩 王卓妮 +5 位作者 史冰柯 王丽 周贝贝 涂洪涛 袁洪波 侯珲 《园艺学报》 北大核心 2026年第1期229-243,共15页
为防治由果生链核盘菌(Monilinia fructicola)引起的桃褐腐病,采用平皿对峙法筛选出拮抗细菌枯草芽孢杆菌J6-1,并分析其对桃褐腐病的防治效果和拮抗机制。结果显示,菌株J6-1对桃褐腐病菌的抑制率达到74%,且具有广谱抑菌活性。菌株J6-1... 为防治由果生链核盘菌(Monilinia fructicola)引起的桃褐腐病,采用平皿对峙法筛选出拮抗细菌枯草芽孢杆菌J6-1,并分析其对桃褐腐病的防治效果和拮抗机制。结果显示,菌株J6-1对桃褐腐病菌的抑制率达到74%,且具有广谱抑菌活性。菌株J6-1能产生蛋白酶、纤维素酶、葡聚糖酶等水解酶,其上清液可以破坏菌丝细胞膜,诱导桃果实病程相关基因(PpPR1)的表达。2%、4%、6%、8%的J6-1上清液对桃褐腐病菌菌丝生长的抑制率分别为64.3%、70.2%、79.75%、85.43%,抑制能力随浓度升高而逐渐增强,且2%的J6-1上清液能引起桃褐腐病菌菌丝变细、消解,原生质外渗,菌丝细胞膜受损。J6-1粗提物中差异幅度最大的前10个差异代谢物主要是氨基酸及其衍生物。桃果实接种M.fructicola 3 d进行菌株J6-1菌悬液、上清液与粗提物处理,抑制效果分别为73.81%、82.48%、92.66%。表明菌株J6-1对桃褐腐病具有良好的防治效果。 展开更多
关键词 褐腐病 枯草芽孢杆菌 生物防治 抑制效果 病程相关基因
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Variation and Probability Grading of Fruit Firmness in Peach Germplasm Resource 被引量:4
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作者 马之胜 王越辉 +2 位作者 贾云云 宣立锋 王建学 《Agricultural Science & Technology》 CAS 2009年第6期91-93,共3页
Objective The aim was to provide basis for further studies on fruit firmness in peach fruits and the standardization and standardization of peach germplasm resource. [ Method] The analysis of fruit firmness of peach g... Objective The aim was to provide basis for further studies on fruit firmness in peach fruits and the standardization and standardization of peach germplasm resource. [ Method] The analysis of fruit firmness of peach germplasm resource was based on the improved firmness measurement, and the probability grading of characteristics was carried out on peach fruit firmness. [Result] The coefficient of variation of peach fruit firmness with skin was less than that of fruit firmness without skin; the fruit firmness with skin and fruit firmness without skin were both fitted the normal distribution; the probability grading of characteristics were divided into five series based on four points of (X-1. 281 8s), (X-0. 524 6s), (X+0. 524 6s) and (X+1.281 8s), so that the probability of 1 -5 were 10%, 20%, 40%, 20% and 10%. [Conclusion] There was more abundant genetic basis in fruit firmness, which held a potential for greater choice. 展开更多
关键词 peach germplasm Fruit firmness VARIATION Probability grading
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河南省桃树流胶病菌的鉴定及其真菌病毒检测
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作者 王彦芬 高许鹏 +5 位作者 陈泽源 翟凤艳 周锋 郭雅双 张猛 余昊 《植物保护》 北大核心 2026年第1期67-78,共12页
为明确河南省桃树流胶病的病原菌组成及其真菌病毒资源,本研究对河南省6个桃园进行病害调查,采用组织分离法获得31个病原菌分离株,经致病力测定和形态学、分子生物学鉴定,确定其分属于4个种:葡萄座腔菌Botryosphaeria dothidea、色二孢D... 为明确河南省桃树流胶病的病原菌组成及其真菌病毒资源,本研究对河南省6个桃园进行病害调查,采用组织分离法获得31个病原菌分离株,经致病力测定和形态学、分子生物学鉴定,确定其分属于4个种:葡萄座腔菌Botryosphaeria dothidea、色二孢Diplodia seriata、可可毛色二孢Lasiodiplodia theobromae和柑橘毛色二孢L.citricola,其中L.citricola为中国桃树新记录种,且B.dothidea为优势病原菌。利用dsRNA和RT-PCR对病原菌携带的真菌病毒进行检测,其中dsRNA检测发现,11个B.dothidea菌株携带0.5~5 kb的dsRNA片段,RT-PCR鉴定出双分体病毒(BdPV1)、多聚真菌病毒(BdRV1)及产黄青霉病毒(BdCV1)。值得注意的是,BdRV1可显著降低寄主真菌的致病力,表明其具有作为桃树流胶病生防制剂的开发潜力。本研究为桃树流胶病的病原学研究和生物防治提供了重要理论基础和资源储备。 展开更多
关键词 桃树流胶病 葡萄座腔菌 鉴定 真菌病毒
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