The molecular basis of orchid flower development involves a specific regulatory program in which MADS-box transcription factors play a central role.The recent‘perianth code’model hypothesizes that two types of highe...The molecular basis of orchid flower development involves a specific regulatory program in which MADS-box transcription factors play a central role.The recent‘perianth code’model hypothesizes that two types of higher-order heterotetrameric complexes,namely SP complex and L complex,play pivotal roles in the orchid perianth organ formation.Therefore,we explored their roles and searched for other components of the regulatory network.Through the combined analysis for transposase-accessible chromatin with high-throughput sequencing and RNA sequencing of the lip-like petal and lip from Phalaenopsis equestris var.trilip,transcription factor-(TF)genes involved in lip development were revealed.PeNAC67 encoding a NAC-type TF and PeSCL23 encoding a GRAS-type TF were differentially expressed between the lip-like petal and the lip.PeNAC67 interacted with and stabilized PeMADS3,which positively regulated the development of lip-like petal to lip.PeSCL23 and PeNAC67 competitively bound with PeKAN2 and positively regulated the development of lip-like petal to petal by affecting the level of PeMADS3.PeKAN2 as an important TF that interacts with PeMADS3 and PeMADS9 can promote lip development.These results extend the‘perianth code’model and shed light on the complex regulation of orchid flower development.展开更多
基金funded by National Key Research and Development Program(grant 2023YFD10005002018YFD000400)Shanghai Engineering Research Center of Plant Germplasm Resources(grant number 17DZ2252700).
文摘The molecular basis of orchid flower development involves a specific regulatory program in which MADS-box transcription factors play a central role.The recent‘perianth code’model hypothesizes that two types of higher-order heterotetrameric complexes,namely SP complex and L complex,play pivotal roles in the orchid perianth organ formation.Therefore,we explored their roles and searched for other components of the regulatory network.Through the combined analysis for transposase-accessible chromatin with high-throughput sequencing and RNA sequencing of the lip-like petal and lip from Phalaenopsis equestris var.trilip,transcription factor-(TF)genes involved in lip development were revealed.PeNAC67 encoding a NAC-type TF and PeSCL23 encoding a GRAS-type TF were differentially expressed between the lip-like petal and the lip.PeNAC67 interacted with and stabilized PeMADS3,which positively regulated the development of lip-like petal to lip.PeSCL23 and PeNAC67 competitively bound with PeKAN2 and positively regulated the development of lip-like petal to petal by affecting the level of PeMADS3.PeKAN2 as an important TF that interacts with PeMADS3 and PeMADS9 can promote lip development.These results extend the‘perianth code’model and shed light on the complex regulation of orchid flower development.
