目的探讨肺腺癌程序性死亡分子-1(programmed death 1,PD-1)、程序性死亡分子配体-1(programmed death ligand 1,PD-L1)蛋白的表达与K-RAS基因突变的相关性。方法采用免疫组化EnVision两步法检测PD-1、PD-L1蛋白的表达,应用实时荧光定量...目的探讨肺腺癌程序性死亡分子-1(programmed death 1,PD-1)、程序性死亡分子配体-1(programmed death ligand 1,PD-L1)蛋白的表达与K-RAS基因突变的相关性。方法采用免疫组化EnVision两步法检测PD-1、PD-L1蛋白的表达,应用实时荧光定量PCR技术检测K-RAS基因的突变类型。结果肺腺癌组织中PD-1、PD-L1的阳性率均高于良性病变肺组织(P<0.01),PD-1、PD-L1蛋白表达与患者性别、年龄、吸烟史、分化程度、淋巴结转移及TNM分期均无相关性(P>0.05)。36例肺腺癌样本中发生K-RAS基因突变者8例(22.2%),K-RAS基因突变与患者性别、年龄、吸烟史、分化程度、淋巴结转移及TNM分期均无关(P>0.05)。相关分析显示PD-1、PD-L1蛋白表达与K-RAS突变无关(P>0.05)。结论 PD-1、PD-L1蛋白在肺腺癌中的表达明显高于良性病变肺组织,但两者表达程度与肺腺癌的临床病理特征及K-RAS基因突变无关。展开更多
The palladium/kieselguhr composites(Pd/K)were prepared by the PdCl_(2) dipping-reducing method.The effects of the preparation conditions on the Pd/K were studied,such as the heat treatment,dipping time,palladium conce...The palladium/kieselguhr composites(Pd/K)were prepared by the PdCl_(2) dipping-reducing method.The effects of the preparation conditions on the Pd/K were studied,such as the heat treatment,dipping time,palladium concentration in solution and number of loading cycles.The pore structure and palladium content of the Pd/K were measured by the Brunauer-Emmett-Teller method and an inductively coupled plasma mass spectrometry.The appearance and palladium element distribution were measured by a scanning electron microscope.It is found that the palladium element is more densely distributed in the irregular and porous parts of the kieselguhr particles,so the kieselguhr is superior to Al_(2)O_(3) as the carrier material.The heat treatment can improve the pore permeability and increase the palladium content for the Pd/K.Increasing the dipping time,palladium concentration in solution and number of loading cycles is beneficial to increase the palladium content of the Pd/K,but more loading cycles may lead to the pore collapse,which obstructs the interaction with the hydrogen isotope gases.A kind of Pd/K was prepared under a set of optimized conditions and was packed in a separation column.This Pd/K was proved to be of high performance and durable by some hydrogen-deuterium separation experiments.展开更多
文摘目的探讨肺腺癌程序性死亡分子-1(programmed death 1,PD-1)、程序性死亡分子配体-1(programmed death ligand 1,PD-L1)蛋白的表达与K-RAS基因突变的相关性。方法采用免疫组化EnVision两步法检测PD-1、PD-L1蛋白的表达,应用实时荧光定量PCR技术检测K-RAS基因的突变类型。结果肺腺癌组织中PD-1、PD-L1的阳性率均高于良性病变肺组织(P<0.01),PD-1、PD-L1蛋白表达与患者性别、年龄、吸烟史、分化程度、淋巴结转移及TNM分期均无相关性(P>0.05)。36例肺腺癌样本中发生K-RAS基因突变者8例(22.2%),K-RAS基因突变与患者性别、年龄、吸烟史、分化程度、淋巴结转移及TNM分期均无关(P>0.05)。相关分析显示PD-1、PD-L1蛋白表达与K-RAS突变无关(P>0.05)。结论 PD-1、PD-L1蛋白在肺腺癌中的表达明显高于良性病变肺组织,但两者表达程度与肺腺癌的临床病理特征及K-RAS基因突变无关。
基金financially supported by the National Natural Science Foundation of China(22109145)the Foundation of Science and Technology on Surface Physics and Chemistry Laboratory(WDZC202202)the Natural Science Foundation of Sichuan Province(2024NSFTD0018)。
文摘The palladium/kieselguhr composites(Pd/K)were prepared by the PdCl_(2) dipping-reducing method.The effects of the preparation conditions on the Pd/K were studied,such as the heat treatment,dipping time,palladium concentration in solution and number of loading cycles.The pore structure and palladium content of the Pd/K were measured by the Brunauer-Emmett-Teller method and an inductively coupled plasma mass spectrometry.The appearance and palladium element distribution were measured by a scanning electron microscope.It is found that the palladium element is more densely distributed in the irregular and porous parts of the kieselguhr particles,so the kieselguhr is superior to Al_(2)O_(3) as the carrier material.The heat treatment can improve the pore permeability and increase the palladium content for the Pd/K.Increasing the dipping time,palladium concentration in solution and number of loading cycles is beneficial to increase the palladium content of the Pd/K,but more loading cycles may lead to the pore collapse,which obstructs the interaction with the hydrogen isotope gases.A kind of Pd/K was prepared under a set of optimized conditions and was packed in a separation column.This Pd/K was proved to be of high performance and durable by some hydrogen-deuterium separation experiments.
