通过激光溅射Pb产生的Pb等离子体与SF_(6)/Ar反应制备了PbF自由基,利用激光诱导荧光光谱测量了PbF分子A^(2)∑^(+)—X^(2)∏_(1/2)跃迁光谱,并精确测得了其A态振动能级v=0和v=1的分子常数T_(0)、B、D、γ和p.此外,还精确测量了PbF分子A^...通过激光溅射Pb产生的Pb等离子体与SF_(6)/Ar反应制备了PbF自由基,利用激光诱导荧光光谱测量了PbF分子A^(2)∑^(+)—X^(2)∏_(1/2)跃迁光谱,并精确测得了其A态振动能级v=0和v=1的分子常数T_(0)、B、D、γ和p.此外,还精确测量了PbF分子A^(2)∑^(+)态v=0振动态的荧光寿命.采用Morse势和RKR(Rydberg-Klein-Rees inversion)/LEVEL方法计算了A^(2)∑^(+)—X^(2)∏_(1/2)跃迁的Franck-Condon因子.计算结果表明,PbF分子不适合进行激光冷却的研究.这不仅丰富了对PbF分子光谱的深入理解,而且为进一步精密测量电子电偶极矩(electronic electric dipole moment,eEDM)提供了重要参考.展开更多
[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring （Triticum aestivum L.）. [Method] Using common wheat Chinese Spring as the experimental material, ...[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring （Triticum aestivum L.）. [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.展开更多
文摘通过激光溅射Pb产生的Pb等离子体与SF_(6)/Ar反应制备了PbF自由基,利用激光诱导荧光光谱测量了PbF分子A^(2)∑^(+)—X^(2)∏_(1/2)跃迁光谱,并精确测得了其A态振动能级v=0和v=1的分子常数T_(0)、B、D、γ和p.此外,还精确测量了PbF分子A^(2)∑^(+)态v=0振动态的荧光寿命.采用Morse势和RKR(Rydberg-Klein-Rees inversion)/LEVEL方法计算了A^(2)∑^(+)—X^(2)∏_(1/2)跃迁的Franck-Condon因子.计算结果表明,PbF分子不适合进行激光冷却的研究.这不仅丰富了对PbF分子光谱的深入理解,而且为进一步精密测量电子电偶极矩(electronic electric dipole moment,eEDM)提供了重要参考.
基金Supported by National Natural Science Foundation of China(30900896)Special Fund for the Construction of Modern Agricultural Technology System(NYCYTX-001)Cyrus Tang Breeding Fund(A212020912)~~
文摘[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring （Triticum aestivum L.）. [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.
