The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make_up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, includi...The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make_up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor_FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber_FISH (EDF_FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3) In situ PCR_based FISH; (4) Bacterial (or yeast) artificial chromosome_FISH (BAC_FISH or YAC_FISH); (5) Tyramide signal amplification_FISH (TSA_FISH); (6) Polypeptide nucleic acid_FISH (PNA_FISH) and (7) padlock_FISH.展开更多
文摘The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make_up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor_FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber_FISH (EDF_FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3) In situ PCR_based FISH; (4) Bacterial (or yeast) artificial chromosome_FISH (BAC_FISH or YAC_FISH); (5) Tyramide signal amplification_FISH (TSA_FISH); (6) Polypeptide nucleic acid_FISH (PNA_FISH) and (7) padlock_FISH.
