Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive com...Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive compounds such as flavonoids and alkaloids,which influence multiple biological pathways.This review aims to summarise and critically analyse recent findings on the pharmacological properties of Passiflora incarnata L.,focusing on its neuropsychiatric,antioxidant,antimicrobial,and anticancer activities.A targeted literature search was conducted in PubMed,Scopus,Web of Science,and Google Scholar for peer-reviewed publications between 2000 to 2025.Relevant articles were screened,and a more appropriate article related to the objective of the review was selected.Some classical papers are also cited as per the requirement of the topic.Passiflora incarnata L.showed multifunctional medicinal properties with various applications in neuropsychiatry,oxidative stress management,antimicrobial agent,and as an anticancer agent.The U.S.Food and Drug Administration categorizes passionflower extracts as“generally recognized as safe”.However,most evidence remains preclinical,with methodological variation limiting generalisation.Standardised formulation,robust clinical trials,and in-depth in vivo studies are essential to establish its therapeutic relevance and safety in modern medicine.展开更多
Based on extensive literature search,the composition,pharmacology,clini cal applications of Passiflora foetida L,ecological characteristics were summarized with the aim to provide scientific data for further research ...Based on extensive literature search,the composition,pharmacology,clini cal applications of Passiflora foetida L,ecological characteristics were summarized with the aim to provide scientific data for further research and exploitation.展开更多
Passiflora species presents a coevolutive relationship with Heliconiini butterflies, their primary herbivores. The Heliconiini caterpillars are able to detoxify toxic compounds produced by Passiflora, thus morphologic...Passiflora species presents a coevolutive relationship with Heliconiini butterflies, their primary herbivores. The Heliconiini caterpillars are able to detoxify toxic compounds produced by Passiflora, thus morphological defense strategies stand out over chemical innovations. In this framework, we highlight the presence of mimetic structures and extrafloral nectaries (EFN) as morphological strategies. Heliconian butterflies oviposit only on leaves that do not possess previous eggs, so the presence of egg mimics could prevent the oviposition. EFN are glands that offer nectar to territorial and aggressive ants, establishing mutualistic relationships. Here, we present a structural and chemical analysis of petiolar EFN and nectar from Passiflora alata and P. edulis in order to have insights about the implications of these features in deterring heliconian caterpillars. P. alata have one to four stipitate-crateriform EFN while P. edulis possess a pair of convex glands. Butterflies lay their eggs isolatedly or in up to three on leaves of both species. Our morphological results suggest that EFN from P. alata may act as egg mimics. Ontogenetic data suggest that the variation in the number of glands observed in this species is a serial homology, wherein the selection pressure for this variation is possibly the oviposition pattern. P. alata retain alkaloids, flavonoids and terpenoids inside nectariferous cells;sugars and flavonoids are found in the nectar of both species, while alkaloids are also detected in P. edulis. There is a selective retention/release of secondary metabolites from the EFN tissues to nectar. Knowing that these compounds can be dissuasive to some herbivores and inoffensive to others, we plotted this relationship in a consumer growth versus secondary metabolite concentration diagram. Our results suggest a more active role in the modulation of the gland defense from plants besides the establishment of a mutualistic relationship with ants, an important response in a coevolutive scenario.展开更多
Objective:To investigate the analgesic and anti-inflammatory activities of ethanol extract of Passiflora foetida(P.foetida) leaves.Methods:Ethanol extract of P.foetida leaf was evaluated for analgesic action by acetic...Objective:To investigate the analgesic and anti-inflammatory activities of ethanol extract of Passiflora foetida(P.foetida) leaves.Methods:Ethanol extract of P.foetida leaf was evaluated for analgesic action by acetic acid-induced writhing and hot plate method in albino mice.The anti-inflammatory property of ethanolic leaf extract was tested by carrageenan induced acute paw edema and histamine induced acute paw edema in rats.Results:The dose 200 mg/kg of P. foetida leaf extract exhibited highest significant analgesic activity[(13.50±0.43) min]at a reaction time of 20 min in hot plate method in mice.The ethanol extract of leaf dose 100 mg/kg produced a highly significant anti inflammatory effect[(1.302±0.079) mL]in rats.Conclusions:It is very clear that P.foetida also has analgesic and anti-inflammatory activities for the pharmaceuticals.展开更多
OBJECTIVE： To investigate analgesic, antidiarrhoeal and cytotoxic activities of the ethanol extract of Passiflora foetida L. （Passifloraceae） by three experimental methods. METHODS： Analgesic activity of the ethan...OBJECTIVE： To investigate analgesic, antidiarrhoeal and cytotoxic activities of the ethanol extract of Passiflora foetida L. （Passifloraceae） by three experimental methods. METHODS： Analgesic activity of the ethanol extract of Passiflora foetida L. （EEPF） was carried out using acetic acid-induced writhing inhibition in mice. The method of castor oil-induced diarrhoea in mice was utilized to evaluate antidiarrhoeal activity. The cytotoxic activity of EEPF was explored with a brine shrimp lethality bioassay. RESULTS： The extract showed 68.75% and 30.00% inhibition of writhe at the doses of 500 and 250 mg/kg body weight, respectively. The extract increased the mean latent period prior to diarrhoeal onset to about 1.55 h and 1.17 h, and decreased the mean number of stools to 4.4 and 5.6 at the doses of 500 and 250 mg/kg body weight. The extract also demonstrated cytotoxic activity in the brine shrimp lethality assay, and the median lethal concentration for brine shrimp nauplii was 80 pg/mL. CONCLUSION： The results suggest that the plant extract has analgesic and antidiarrhoeal activities, supporting its uses in traditional medicine. The results also demonstrate that the plant extract possesses cytotoxic activities.展开更多
Banana passion fruit (P. tripartita var. Mollissima) is one of the most promising tropical fruits giving its antioxidant activity (AOA) to replace synthetic additives. Despite this property, there are no studies about...Banana passion fruit (P. tripartita var. Mollissima) is one of the most promising tropical fruits giving its antioxidant activity (AOA) to replace synthetic additives. Despite this property, there are no studies about the metabolites responsible for its biological function or proposals for the application of technologies, such as microencapsulation by spray drying, to improve its properties and ease its incorporation in several food matrices. The aim of this study is to microencapsulate the pulp of banana passion fruit with several mixtures of encapsulants and identify which one of these mixtures is better to preserve its AOA. The antioxidant activity values for the banana passion fruit pulp were as follows: DPPH: 6630.2 ± 91 μMtrolox/100g;ABTS: 18764.3 ± 270.4 μMtrolox/100g;FRAP: 1703.6± 938.2 mgAA/100g, ORAC: 8105.4 ± 424.2 μmol TEAC/100g of sample;Total phenols: 8862.2 ± 451.4 gallic ac. mg/100g. The concentrations of the bioactive compounds expressed in mg of gallic acid per 100 g of the pulp on a dry base were 13.9 ± 0.004;5.9 ± 0.001 and 126.3 ± 0.004 for caffeic, p-coumaric and ferulic acids, respectively. The best shelf-life followed by ABTS in eight assays was between 28.8 and 31.5 weeks using maltodextrin and modified starch, MD:MS (1/4:3/4) and MD:MS (0:1), respectively. In conclusion, ABTS is the best method to measure the AOA in banana passion fruit because it correlated with the phenolic compounds better than DPPH and FRAP methods. Additionally, two options were found to protect the AOA and to extent the shelf-life of the passion fruit by spray-drying, with mixtures of encapsulants widely used in the food industry.展开更多
A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5 α-cholestane-3 β ,6 a -diol 2. The structure of passionsterone 1 was eluci...A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5 α-cholestane-3 β ,6 a -diol 2. The structure of passionsterone 1 was elucidated as 24R-ethyl-5 α -cholestane-3 β,6 α-diol-23-one by spectroscopic means.展开更多
Objective: To establish a simple, rapid, precise and accurate high performance thin layer chromatography(HPTLC) method with densitometric detection for the determination of vitexin in Passiflora foetida Linn.(P. foeti...Objective: To establish a simple, rapid, precise and accurate high performance thin layer chromatography(HPTLC) method with densitometric detection for the determination of vitexin in Passiflora foetida Linn.(P. foetida).Methods: Ethanolic extract of the plant leaf powder was used for the experimental work.Separation was performed on silica gel 60 F254 HPTLC plates with ethyl acetate:methanol: distilled water: formic acid in the proportion of 50:2:3:6(v/v), as the mobile phase. The determination was carried out using the densitometric absorbance mode at340 nm.Results: Vitexin response was linear over the range of 2.5–17.5 mg/m L with a correlation coefficient of 0.996. Intraday and interday precision studies showed the relative SD was< 3%. Accuracy of the method was determined and the average recovery was 100.3%.The limit of quantitation and limit of detection were 0.879 and 0.290 mg/m L, respectively.The contents of vitexin in P. foetida leaf extracts were within the range of 0.030%–0.310%.Conclusions: The method was evaluated for sensitivity, accuracy, precision and reproducibility. Each analysis by HPTLC is less expensive than current methods. This method is suitable for routine quality control of raw material of the leaves of P. foetida extract and its products.展开更多
Objective:To investigate the effect of administration of Passiflora glandulosa(P.glandulosa) fruit rinds flour on streptozotocin(STZ)-induced diabetic mice.Methods:The preliminary phytochemical screening and parameter...Objective:To investigate the effect of administration of Passiflora glandulosa(P.glandulosa) fruit rinds flour on streptozotocin(STZ)-induced diabetic mice.Methods:The preliminary phytochemical screening and parameters such as centesimal composition and brine shrimp toxicity were evaluated.For in vivo study Swiss female mice were divided into four groups:NC-normal control;DC-diabetic control animals receiving saline;MET-diabetic animals receiving metformin(200 mg/kg);PFRF-diabetic animals receiving P.glandulosa fruit rinds flour(200 mg/kg).All of them were treated for 28 d.STZ was used in a single dose of 120 mg/kg to establish diabetic models.Body weight,water and food intake,fasting blood glucose were measured.Histopathological analysis of pancreas and liver were performed to evaluate STZ-induced tissue injuries.Results:Phytochemical screening showed the presence of flavanones and triterpenoids.The P.glandulosa fruit rinds flour was non-toxic by the brine shrimp test.The fruit rinds flour also reduced the loss of body weight and significantly decreased food intake in the diabetic mice.Additionally,a significant reduction in blood glucose was observed for 15 d and this was maintained on 21 d and 28 d when compared with diabetic mice.Furthermore,the P.glandulosa fruit rinds flour has a favourable effect on the histopathological changes of the pancreas in STZ induced diabetes.Conclusions:It is concluded that P.glandulosa fruit rinds flour is a natural product that contains potent antioxidant compounds and presents good prospects for the improvement of diabetic mellitus by reducing serum glucose levels.展开更多
In recent years,the development trend of passion fruit seedling industry in Qinzhou,Guangxi is good,but on the whole,it is still in its infancy and the development of the industry is fragile.This paper briefly describ...In recent years,the development trend of passion fruit seedling industry in Qinzhou,Guangxi is good,but on the whole,it is still in its infancy and the development of the industry is fragile.This paper briefly described the development status and existing problems of the passion fruit seedling industry in Qinzhou,Guangxi,and put forward reasonable suggestions in order to promote the healthy development of the passion fruit seedling industry,increase farmers’income and prosper rural economy.展开更多
The Scab of purple passion fruit or gulupa (Passiflora edulis Sims.) is a serious disease, affecting all aerial organs of the plant, but also to reducing the visual quality of the fruits, which reduces export producti...The Scab of purple passion fruit or gulupa (Passiflora edulis Sims.) is a serious disease, affecting all aerial organs of the plant, but also to reducing the visual quality of the fruits, which reduces export production. To determine the primary causal agent of disease in Colombia, associated microorganisms were isolated from scab lesions on stems, leaves, flowers and fruits, recovering in PDA. Obtaining Cladosporium, Colletotrichum and Botrytis. Cladosporium had the highest frequency of fungal isolates. 15 strains were evaluated for their pathogenicity on fruits and leaves of gulupa in laboratory and greenhouse conditions. 9 strains caused symptom expression at 95% humidity and 17°C - 20°C, validating Cladosporium as causal agent of gulupa scab. For those pathogenic strains, were determinate incubation and latency periodsin leaves (7 - 11 and 8 - 12 DPI) and fruits (6 - 10 and 8 - 12 DPI) revealing differences or virulence levels in the pathogen populations.展开更多
This study aimed to determine structural characteristics, physical properties and binding effects of polysaccharide and its fractions from Passiflora edulis peel on lipid, cholesterol and bile acids. The crude polysac...This study aimed to determine structural characteristics, physical properties and binding effects of polysaccharide and its fractions from Passiflora edulis peel on lipid, cholesterol and bile acids. The crude polysaccharide (WPEP) was extracted using hot water extraction method, fractionated using DEAE-52 cellulose column, and purified by Sepharose CL-6B column to obtain four polysaccharide fractions (WPEP-F1, WPEP-F2, WPEP-F3, and WPEP-F4). The physicochemical properties and structural characteristics were analyzed using several methods. The chemical-binding effects were also determined. Among all polysaccharide samples, WPEP-F2 had the highest total sugar content (77.63%). The viscosity of all polysaccharide samples was increased. Among the four polysaccharide fractions, WPEP-F2 showed the best emulsifying activity. WPEP-F1 was mainly composed of glucose, galactose, mannose, and arabinose, whereas WPEP-F2, WPEP-F3, and WPEP-F4 were principally composed of galacturonic acid. SEM analysis showed that the four fractions had different microstructures. Among the polysaccharide components, WPEP-F2 had the highest lipid-binding effect (9.3 g/g). It also had the best binding effect on taurocholic acid, deoxycholic acid, glycocholic acid, and cholesterol (50.87%, 69.12%, 30.22%, and 78.68%, respectively), but WPEP-F3 had the best binding effect on cholic acid (53.99%). The lipid, cholesterol, and bile acid-binding activities of WPEP-F2 could be attributed to its high viscosity, porous appearance structure, and sugar content in addition to its triple helix structure. The findings lay a theoretical foundation for developing Passiflora edulis peel polysaccharide-related foods and promoting application of the peel polysaccharide in functional food industries.展开更多
The present report describes the development of quantitative and qualitative foliar micromorphological and architectural features in the field environment which elucidated the adaptation of micropropagated plants of P...The present report describes the development of quantitative and qualitative foliar micromorphological and architectural features in the field environment which elucidated the adaptation of micropropagated plants of Passiflora foetida L. in the natural soil conditions. The field environment(high light intensity in comparison to in vitro culture conditions) promotes the autotrophy through decrease in stomatal index(from 23.2 ± 0.15 to 21.0 ± 0.19), increased vein-islets(from 10.0 ± 0.14 to 15.6 ± 0.24 per square millimeters) and veinlet terminations(from 1.6 ± 0.14 to 5.0 ± 0.20 per square millimeters), and trichome density in P. foetida plantlets. The in vitro and field grown leaves mostly possessed anomocytic and anisocytic types of stomata. Two types of trichomes were observed on the surface of leaves of in vitro as well as field transferred plants of P. foetida: the unicellular hairy trichomes(non-glandular), and the multicellular(glandular) trichomes. The trichomes density was less under in vitro conditions as compared to the in vivo environments. The new leaves formed during the ex vitro rooting stage(in greenhouse) and after transplantation of plantlets to the field exhibited the development of adaptive micromorphological features in micropropagated plants, which enabled them to survive under field conditions.展开更多
Passiflora is a plant genus known for its extremely distinctive and colorful flowers and a wide range of genome size variation.However,how genome characteristics are related to flower traits among Passiflora species r...Passiflora is a plant genus known for its extremely distinctive and colorful flowers and a wide range of genome size variation.However,how genome characteristics are related to flower traits among Passiflora species remains poorly understood.Here,we assembled a chromosome-scale genome of P.foetida,which belongs to the same subgenus as the commercial passionfruit P.edulis.The genome of P.foetida is smaller(424.16 Mb)and contains fewer copies of long terminal repeat retrotransposons(LTR-RTs).The disparity in LTR-RTs is one of the main contributors to the differences in genome sizes between these two species and possibly in floral traits.Additionally,we observed variation in insertion times and copy numbers of LTR-RTs across different transposable element(TE)lineages.Then,by integrating transcriptomic data from 33 samples(eight floral organs and flower buds at three developmental stages)with phylogenomic and metabolomic data,we conducted an in-depth analysis of the expression,phylogeny,and copy number of MIKC-type MADS-box genes and identified essential biosynthetic genes responsible for flower color and scent from glandular bracts and other floral organs.Our study pinpoints LRT-RTs as an important player in genome size variation in Passiflora species and provides insights into future genetic improvement.展开更多
文摘Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive compounds such as flavonoids and alkaloids,which influence multiple biological pathways.This review aims to summarise and critically analyse recent findings on the pharmacological properties of Passiflora incarnata L.,focusing on its neuropsychiatric,antioxidant,antimicrobial,and anticancer activities.A targeted literature search was conducted in PubMed,Scopus,Web of Science,and Google Scholar for peer-reviewed publications between 2000 to 2025.Relevant articles were screened,and a more appropriate article related to the objective of the review was selected.Some classical papers are also cited as per the requirement of the topic.Passiflora incarnata L.showed multifunctional medicinal properties with various applications in neuropsychiatry,oxidative stress management,antimicrobial agent,and as an anticancer agent.The U.S.Food and Drug Administration categorizes passionflower extracts as“generally recognized as safe”.However,most evidence remains preclinical,with methodological variation limiting generalisation.Standardised formulation,robust clinical trials,and in-depth in vivo studies are essential to establish its therapeutic relevance and safety in modern medicine.
基金Supported by the Key Laboratory of Resource Biology and Biotechnology in Western China of Ministry of Education (KH09030)the Special Fund for the Science and Technology of Science and Technology Department of Tibet Autonomous Region (20091012)the Scientific Research Program Funded by Shaanxi Provincial Education Department (2010JK862)~~
文摘Based on extensive literature search,the composition,pharmacology,clini cal applications of Passiflora foetida L,ecological characteristics were summarized with the aim to provide scientific data for further research and exploitation.
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq 131813/2008-0).
文摘Passiflora species presents a coevolutive relationship with Heliconiini butterflies, their primary herbivores. The Heliconiini caterpillars are able to detoxify toxic compounds produced by Passiflora, thus morphological defense strategies stand out over chemical innovations. In this framework, we highlight the presence of mimetic structures and extrafloral nectaries (EFN) as morphological strategies. Heliconian butterflies oviposit only on leaves that do not possess previous eggs, so the presence of egg mimics could prevent the oviposition. EFN are glands that offer nectar to territorial and aggressive ants, establishing mutualistic relationships. Here, we present a structural and chemical analysis of petiolar EFN and nectar from Passiflora alata and P. edulis in order to have insights about the implications of these features in deterring heliconian caterpillars. P. alata have one to four stipitate-crateriform EFN while P. edulis possess a pair of convex glands. Butterflies lay their eggs isolatedly or in up to three on leaves of both species. Our morphological results suggest that EFN from P. alata may act as egg mimics. Ontogenetic data suggest that the variation in the number of glands observed in this species is a serial homology, wherein the selection pressure for this variation is possibly the oviposition pattern. P. alata retain alkaloids, flavonoids and terpenoids inside nectariferous cells;sugars and flavonoids are found in the nectar of both species, while alkaloids are also detected in P. edulis. There is a selective retention/release of secondary metabolites from the EFN tissues to nectar. Knowing that these compounds can be dissuasive to some herbivores and inoffensive to others, we plotted this relationship in a consumer growth versus secondary metabolite concentration diagram. Our results suggest a more active role in the modulation of the gland defense from plants besides the establishment of a mutualistic relationship with ants, an important response in a coevolutive scenario.
基金supported by University Grants Commission New Delhi(UGC letter No:F.No.37-95/2009(SR)
文摘Objective:To investigate the analgesic and anti-inflammatory activities of ethanol extract of Passiflora foetida(P.foetida) leaves.Methods:Ethanol extract of P.foetida leaf was evaluated for analgesic action by acetic acid-induced writhing and hot plate method in albino mice.The anti-inflammatory property of ethanolic leaf extract was tested by carrageenan induced acute paw edema and histamine induced acute paw edema in rats.Results:The dose 200 mg/kg of P. foetida leaf extract exhibited highest significant analgesic activity[(13.50±0.43) min]at a reaction time of 20 min in hot plate method in mice.The ethanol extract of leaf dose 100 mg/kg produced a highly significant anti inflammatory effect[(1.302±0.079) mL]in rats.Conclusions:It is very clear that P.foetida also has analgesic and anti-inflammatory activities for the pharmaceuticals.
文摘OBJECTIVE： To investigate analgesic, antidiarrhoeal and cytotoxic activities of the ethanol extract of Passiflora foetida L. （Passifloraceae） by three experimental methods. METHODS： Analgesic activity of the ethanol extract of Passiflora foetida L. （EEPF） was carried out using acetic acid-induced writhing inhibition in mice. The method of castor oil-induced diarrhoea in mice was utilized to evaluate antidiarrhoeal activity. The cytotoxic activity of EEPF was explored with a brine shrimp lethality bioassay. RESULTS： The extract showed 68.75% and 30.00% inhibition of writhe at the doses of 500 and 250 mg/kg body weight, respectively. The extract increased the mean latent period prior to diarrhoeal onset to about 1.55 h and 1.17 h, and decreased the mean number of stools to 4.4 and 5.6 at the doses of 500 and 250 mg/kg body weight. The extract also demonstrated cytotoxic activity in the brine shrimp lethality assay, and the median lethal concentration for brine shrimp nauplii was 80 pg/mL. CONCLUSION： The results suggest that the plant extract has analgesic and antidiarrhoeal activities, supporting its uses in traditional medicine. The results also demonstrate that the plant extract possesses cytotoxic activities.
文摘Banana passion fruit (P. tripartita var. Mollissima) is one of the most promising tropical fruits giving its antioxidant activity (AOA) to replace synthetic additives. Despite this property, there are no studies about the metabolites responsible for its biological function or proposals for the application of technologies, such as microencapsulation by spray drying, to improve its properties and ease its incorporation in several food matrices. The aim of this study is to microencapsulate the pulp of banana passion fruit with several mixtures of encapsulants and identify which one of these mixtures is better to preserve its AOA. The antioxidant activity values for the banana passion fruit pulp were as follows: DPPH: 6630.2 ± 91 μMtrolox/100g;ABTS: 18764.3 ± 270.4 μMtrolox/100g;FRAP: 1703.6± 938.2 mgAA/100g, ORAC: 8105.4 ± 424.2 μmol TEAC/100g of sample;Total phenols: 8862.2 ± 451.4 gallic ac. mg/100g. The concentrations of the bioactive compounds expressed in mg of gallic acid per 100 g of the pulp on a dry base were 13.9 ± 0.004;5.9 ± 0.001 and 126.3 ± 0.004 for caffeic, p-coumaric and ferulic acids, respectively. The best shelf-life followed by ABTS in eight assays was between 28.8 and 31.5 weeks using maltodextrin and modified starch, MD:MS (1/4:3/4) and MD:MS (0:1), respectively. In conclusion, ABTS is the best method to measure the AOA in banana passion fruit because it correlated with the phenolic compounds better than DPPH and FRAP methods. Additionally, two options were found to protect the AOA and to extent the shelf-life of the passion fruit by spray-drying, with mixtures of encapsulants widely used in the food industry.
文摘A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5 α-cholestane-3 β ,6 a -diol 2. The structure of passionsterone 1 was elucidated as 24R-ethyl-5 α -cholestane-3 β,6 α-diol-23-one by spectroscopic means.
文摘Objective: To establish a simple, rapid, precise and accurate high performance thin layer chromatography(HPTLC) method with densitometric detection for the determination of vitexin in Passiflora foetida Linn.(P. foetida).Methods: Ethanolic extract of the plant leaf powder was used for the experimental work.Separation was performed on silica gel 60 F254 HPTLC plates with ethyl acetate:methanol: distilled water: formic acid in the proportion of 50:2:3:6(v/v), as the mobile phase. The determination was carried out using the densitometric absorbance mode at340 nm.Results: Vitexin response was linear over the range of 2.5–17.5 mg/m L with a correlation coefficient of 0.996. Intraday and interday precision studies showed the relative SD was< 3%. Accuracy of the method was determined and the average recovery was 100.3%.The limit of quantitation and limit of detection were 0.879 and 0.290 mg/m L, respectively.The contents of vitexin in P. foetida leaf extracts were within the range of 0.030%–0.310%.Conclusions: The method was evaluated for sensitivity, accuracy, precision and reproducibility. Each analysis by HPTLC is less expensive than current methods. This method is suitable for routine quality control of raw material of the leaves of P. foetida extract and its products.
基金finacially supported by GBtech(GreenBean Biotecnologia)
文摘Objective:To investigate the effect of administration of Passiflora glandulosa(P.glandulosa) fruit rinds flour on streptozotocin(STZ)-induced diabetic mice.Methods:The preliminary phytochemical screening and parameters such as centesimal composition and brine shrimp toxicity were evaluated.For in vivo study Swiss female mice were divided into four groups:NC-normal control;DC-diabetic control animals receiving saline;MET-diabetic animals receiving metformin(200 mg/kg);PFRF-diabetic animals receiving P.glandulosa fruit rinds flour(200 mg/kg).All of them were treated for 28 d.STZ was used in a single dose of 120 mg/kg to establish diabetic models.Body weight,water and food intake,fasting blood glucose were measured.Histopathological analysis of pancreas and liver were performed to evaluate STZ-induced tissue injuries.Results:Phytochemical screening showed the presence of flavanones and triterpenoids.The P.glandulosa fruit rinds flour was non-toxic by the brine shrimp test.The fruit rinds flour also reduced the loss of body weight and significantly decreased food intake in the diabetic mice.Additionally,a significant reduction in blood glucose was observed for 15 d and this was maintained on 21 d and 28 d when compared with diabetic mice.Furthermore,the P.glandulosa fruit rinds flour has a favourable effect on the histopathological changes of the pancreas in STZ induced diabetes.Conclusions:It is concluded that P.glandulosa fruit rinds flour is a natural product that contains potent antioxidant compounds and presents good prospects for the improvement of diabetic mellitus by reducing serum glucose levels.
基金Supported by Science and Technology Pioneer"Strengthening Farmers and Enriching People"and"Six Ones"Special Action Project(GNKM 202104)Grassroots Agricultural Technology Extension Service Ability Improvement Project of Agriculture and Rural Affairs Department,Guangxi Zhuang Autonomous Region。
文摘In recent years,the development trend of passion fruit seedling industry in Qinzhou,Guangxi is good,but on the whole,it is still in its infancy and the development of the industry is fragile.This paper briefly described the development status and existing problems of the passion fruit seedling industry in Qinzhou,Guangxi,and put forward reasonable suggestions in order to promote the healthy development of the passion fruit seedling industry,increase farmers’income and prosper rural economy.
文摘The Scab of purple passion fruit or gulupa (Passiflora edulis Sims.) is a serious disease, affecting all aerial organs of the plant, but also to reducing the visual quality of the fruits, which reduces export production. To determine the primary causal agent of disease in Colombia, associated microorganisms were isolated from scab lesions on stems, leaves, flowers and fruits, recovering in PDA. Obtaining Cladosporium, Colletotrichum and Botrytis. Cladosporium had the highest frequency of fungal isolates. 15 strains were evaluated for their pathogenicity on fruits and leaves of gulupa in laboratory and greenhouse conditions. 9 strains caused symptom expression at 95% humidity and 17°C - 20°C, validating Cladosporium as causal agent of gulupa scab. For those pathogenic strains, were determinate incubation and latency periodsin leaves (7 - 11 and 8 - 12 DPI) and fruits (6 - 10 and 8 - 12 DPI) revealing differences or virulence levels in the pathogen populations.
基金supported by the National Natural Science Foundation of China(31860251)the Natural Science Foundation of Guangxi Province(2021GXNSFAA220072)+1 种基金the Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials(EMFM20211104)the Guangxi Science&Technology Program(AD20297088).
文摘This study aimed to determine structural characteristics, physical properties and binding effects of polysaccharide and its fractions from Passiflora edulis peel on lipid, cholesterol and bile acids. The crude polysaccharide (WPEP) was extracted using hot water extraction method, fractionated using DEAE-52 cellulose column, and purified by Sepharose CL-6B column to obtain four polysaccharide fractions (WPEP-F1, WPEP-F2, WPEP-F3, and WPEP-F4). The physicochemical properties and structural characteristics were analyzed using several methods. The chemical-binding effects were also determined. Among all polysaccharide samples, WPEP-F2 had the highest total sugar content (77.63%). The viscosity of all polysaccharide samples was increased. Among the four polysaccharide fractions, WPEP-F2 showed the best emulsifying activity. WPEP-F1 was mainly composed of glucose, galactose, mannose, and arabinose, whereas WPEP-F2, WPEP-F3, and WPEP-F4 were principally composed of galacturonic acid. SEM analysis showed that the four fractions had different microstructures. Among the polysaccharide components, WPEP-F2 had the highest lipid-binding effect (9.3 g/g). It also had the best binding effect on taurocholic acid, deoxycholic acid, glycocholic acid, and cholesterol (50.87%, 69.12%, 30.22%, and 78.68%, respectively), but WPEP-F3 had the best binding effect on cholic acid (53.99%). The lipid, cholesterol, and bile acid-binding activities of WPEP-F2 could be attributed to its high viscosity, porous appearance structure, and sugar content in addition to its triple helix structure. The findings lay a theoretical foundation for developing Passiflora edulis peel polysaccharide-related foods and promoting application of the peel polysaccharide in functional food industries.
基金financial support to our laboratory as Major Research Project and Grant-In-Aid Scheme (No. 10/DSTE/GIA/RP/JSA-I/2013/213)
文摘The present report describes the development of quantitative and qualitative foliar micromorphological and architectural features in the field environment which elucidated the adaptation of micropropagated plants of Passiflora foetida L. in the natural soil conditions. The field environment(high light intensity in comparison to in vitro culture conditions) promotes the autotrophy through decrease in stomatal index(from 23.2 ± 0.15 to 21.0 ± 0.19), increased vein-islets(from 10.0 ± 0.14 to 15.6 ± 0.24 per square millimeters) and veinlet terminations(from 1.6 ± 0.14 to 5.0 ± 0.20 per square millimeters), and trichome density in P. foetida plantlets. The in vitro and field grown leaves mostly possessed anomocytic and anisocytic types of stomata. Two types of trichomes were observed on the surface of leaves of in vitro as well as field transferred plants of P. foetida: the unicellular hairy trichomes(non-glandular), and the multicellular(glandular) trichomes. The trichomes density was less under in vitro conditions as compared to the in vivo environments. The new leaves formed during the ex vitro rooting stage(in greenhouse) and after transplantation of plantlets to the field exhibited the development of adaptive micromorphological features in micropropagated plants, which enabled them to survive under field conditions.
基金funded by the Guangdong Pearl River Talent Program(grants 2021QN02N792)the Shenzhen Fundamental Research Program(grants JCYJ20220818103212025)+4 种基金the Science,Technology and Innovation Commission of Shenzhen Municipality(STIC:RCYX20200714114538196)the Scientific Research Foundation for Principle Investigator from Kunpeng Institute of Modern Agriculture at Foshan(grants KIMA-QD2022004)the Chinese Academy of Agricultural Sciences Elite Youth Program(grants 110243160001007)the National Natural Science Foundation of China(32100184)the Grants from Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology(YNPRAEC-2023001).
文摘Passiflora is a plant genus known for its extremely distinctive and colorful flowers and a wide range of genome size variation.However,how genome characteristics are related to flower traits among Passiflora species remains poorly understood.Here,we assembled a chromosome-scale genome of P.foetida,which belongs to the same subgenus as the commercial passionfruit P.edulis.The genome of P.foetida is smaller(424.16 Mb)and contains fewer copies of long terminal repeat retrotransposons(LTR-RTs).The disparity in LTR-RTs is one of the main contributors to the differences in genome sizes between these two species and possibly in floral traits.Additionally,we observed variation in insertion times and copy numbers of LTR-RTs across different transposable element(TE)lineages.Then,by integrating transcriptomic data from 33 samples(eight floral organs and flower buds at three developmental stages)with phylogenomic and metabolomic data,we conducted an in-depth analysis of the expression,phylogeny,and copy number of MIKC-type MADS-box genes and identified essential biosynthetic genes responsible for flower color and scent from glandular bracts and other floral organs.Our study pinpoints LRT-RTs as an important player in genome size variation in Passiflora species and provides insights into future genetic improvement.
