Objective:To evaluate the in vivo trypanocidal activity of the methanol extract and fractions of Abrus precatorius seeds in mice.Methods:Parasiteamia was induced unto mice by intraperitoneal injection of 1.25×10&...Objective:To evaluate the in vivo trypanocidal activity of the methanol extract and fractions of Abrus precatorius seeds in mice.Methods:Parasiteamia was induced unto mice by intraperitoneal injection of 1.25×10<sup>5</sup> Trypanosoma in normal saline.Five days when a high level of parasiteamia was established treatment commenced until ten days.The mice were treated with 10,20 and 40 mg/kg bt.of the extract and 5 and 10 mg/kg bt.of the fraction（F<sub>2</sub>）,respectively for 5 days.Diminazene acelurate at the dose of 3.5 mg/kg bt.for two days was used as the reference drug.The level of parasitaemia and packed cell volume（PCV） of the animals estimated. Results:At doses of 10,20 and 40 mg/kg the crude extract showed a sharp reduction in the level of parasitaemia in mice compared with the untreated group.The mice treated with F,at doses of 5 and 10 mg/kg showed a sharp reduction in the level of parasitamia to zero in day 9,and a gradual recovery from the 12th day of treatment.This effect is comparable to that of the mice treated with 7 mg/kg of standard drug diminazene aceturate.The PCV of the treated showed a gradual decrease with time,but not as much as the untreated group.Phytochemical screening revealed the presence of glycosides,alkaloids,carbohydrates,tannins and proteins in the Abrus precatorius powder while F<sub>2</sub> was rich in alkaloids.Conclusions:This study shows that both the extract and the fractions of Abrus precatorius seeds exhibited a promising trypanocidal property.Alkaloids may be responsible for the observed activity.展开更多
Objective:To investigate the relationship between malaria parasite status and cholesterol level of 110 consenting subjects(55 patients and 55 controls) in parts of the Imo River Basin of Nigeria. Methods:Giemsa staini...Objective:To investigate the relationship between malaria parasite status and cholesterol level of 110 consenting subjects(55 patients and 55 controls) in parts of the Imo River Basin of Nigeria. Methods:Giemsa staining was used for malaria parasite examination while Randox cholesterol kit was used for cholesterol level estimation.Results:About 49 persons(90%) with malaria had low cholesterol(【180 mg/dL).Highest mean cholesterol levels were 274 mg/dL for study patients and 220 mg/dL for controls respectively;Lowest mean cholesterol levels were 168 mg/dL (patienls) and 138 mg/dL(controls) respectively.Low cholesterol levels(【 180 mg/dL) were found in patients(84%) and controls(6%) respectively.However.16.4%of controls and 6%of patients had borderline cholesterol level(200-239 mg/dL).This study establishes a significant correlation(12.9%.P【0.01) between malaria parasite status and cholesterol level.Conclusions: These findings imply that choleslerol level estimation may be a potential concurrent and valuable diagnosis for malaria status.展开更多
文摘Objective:To evaluate the in vivo trypanocidal activity of the methanol extract and fractions of Abrus precatorius seeds in mice.Methods:Parasiteamia was induced unto mice by intraperitoneal injection of 1.25×10<sup>5</sup> Trypanosoma in normal saline.Five days when a high level of parasiteamia was established treatment commenced until ten days.The mice were treated with 10,20 and 40 mg/kg bt.of the extract and 5 and 10 mg/kg bt.of the fraction（F<sub>2</sub>）,respectively for 5 days.Diminazene acelurate at the dose of 3.5 mg/kg bt.for two days was used as the reference drug.The level of parasitaemia and packed cell volume（PCV） of the animals estimated. Results:At doses of 10,20 and 40 mg/kg the crude extract showed a sharp reduction in the level of parasitaemia in mice compared with the untreated group.The mice treated with F,at doses of 5 and 10 mg/kg showed a sharp reduction in the level of parasitamia to zero in day 9,and a gradual recovery from the 12th day of treatment.This effect is comparable to that of the mice treated with 7 mg/kg of standard drug diminazene aceturate.The PCV of the treated showed a gradual decrease with time,but not as much as the untreated group.Phytochemical screening revealed the presence of glycosides,alkaloids,carbohydrates,tannins and proteins in the Abrus precatorius powder while F<sub>2</sub> was rich in alkaloids.Conclusions:This study shows that both the extract and the fractions of Abrus precatorius seeds exhibited a promising trypanocidal property.Alkaloids may be responsible for the observed activity.
文摘Objective:To investigate the relationship between malaria parasite status and cholesterol level of 110 consenting subjects(55 patients and 55 controls) in parts of the Imo River Basin of Nigeria. Methods:Giemsa staining was used for malaria parasite examination while Randox cholesterol kit was used for cholesterol level estimation.Results:About 49 persons(90%) with malaria had low cholesterol(【180 mg/dL).Highest mean cholesterol levels were 274 mg/dL for study patients and 220 mg/dL for controls respectively;Lowest mean cholesterol levels were 168 mg/dL (patienls) and 138 mg/dL(controls) respectively.Low cholesterol levels(【 180 mg/dL) were found in patients(84%) and controls(6%) respectively.However.16.4%of controls and 6%of patients had borderline cholesterol level(200-239 mg/dL).This study establishes a significant correlation(12.9%.P【0.01) between malaria parasite status and cholesterol level.Conclusions: These findings imply that choleslerol level estimation may be a potential concurrent and valuable diagnosis for malaria status.
