[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and ...[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and a new method termed＆quot;opening a window on cornea and refixation＆quot; was used to prepare paraffin sections for cattle eyebal s in the treatment group. [Result] After the prepared specimens in the treatment group were fixed, it could be macroscopical y observed that retina and choroid were closely connected, with detachment occurring at a smal portion be-tween the two. According to the paraffin sections, it was microscopical y observed that the continuity of trabecular meshwork was intact, as wel as the continuity be-tween different layers of eyebal wal , without detachment between them, no retinal detachment, no shrinkage of each layer of tissue cells. [Conclusion] This study pro-vides a foundation for the basic research and pathological study of eyebal s.展开更多
Chimonanthus praecox is an important ornamental plant and cut flower material in China.It blooms in the freezing winter and its flower emits charming fragrance.However,in different region the flowering time is variabl...Chimonanthus praecox is an important ornamental plant and cut flower material in China.It blooms in the freezing winter and its flower emits charming fragrance.However,in different region the flowering time is variable.In order to understand the flowering mechanism of Ch.praecox in the winter,we studied the flower bud differentiation in Spring City-Kunming using paraffin sectioning method in the present study.Meanwhile we compared the differentiation process difference from different regions.It was found that the temperature is the key factor for its flower bud differentiation and blossom of Ch.praecox.In the process of bud differentiation,the temperature 20℃was the optimum for inducing changes from vegetative axillary buds to reproductive buds and subsequent morphological differentiation in Ch.praecox.Furthermore in the first three differentiation periods—tepal primordial stage,staminal primordial stage and pistil primordial stage,Kunming took the shortest time to finish the process due to very rapid temperature rise to 20℃,whereas,in Zhengzhou the time for these differentiations was the longest,which may be caused by the slow temperature rise.After May,the high temperature stress forced the flower buds into the first long dormant period in all regions except Kunming.In Kunming,the average temperature was only 20–25℃,so the flower bud continued to differentiate.In all regions,Kunming is the first to complete whole flower bud differentiation even on the early August,and started the second dormancy very early but very long.In the other regions,the plants went through a shorter dormancy and the low temperature broke the dormancy rapidly.Contrarily the plants of Kunming spent a longer period for the low temperature.Thus,the low temperature less than 10℃is a key factor to breaking the second dormancy.Surely the regular effects of temperature on flower bud differentiation and blossom is very helpful for florescence regulation of Ch.praecox.展开更多
[Objective] Taking Pinus sylvestris needles as materials, a set of test con-ditions suitable for the preparation of paraffin sections of Pinus sylvestris needles was determined. [Method] Based on the traditional metho...[Objective] Taking Pinus sylvestris needles as materials, a set of test con-ditions suitable for the preparation of paraffin sections of Pinus sylvestris needles was determined. [Method] Based on the traditional method for paraffin sections preparation, steps including fixation, dehydration, adhering to slides and staining were investigated taking the structural characteristics of pine needles into considera-tion. [Result] 70% ethanol was used in the FAA fixative; before affixed on slides, the cut sections were first expanded in a 40 ℃ water bath and taken out by using Su-perfrost Plus slides, fol owed by drying in a 35 ℃ oven for 24 h; pine needles were cut into 8 μm thick sections; staining was achieved by immersion in eosin for 5 min and in hematoxylin for 1 min. [Conclusion] The conditions obtained above can en-hance the effect of fixation and dehydration; adhering to slides is easy to operate and the sections are not easy to drop; the dyeing effect is relatively preferable.展开更多
[Objective] The purpose was to introduce a preparation method for paraffin section of Bemisia tabaci, so as to lay the foundation for the studies on changes of organizational structure of B. tabaci. [ Method] The tech...[Objective] The purpose was to introduce a preparation method for paraffin section of Bemisia tabaci, so as to lay the foundation for the studies on changes of organizational structure of B. tabaci. [ Method] The technique of paraffin section and the method of H-E staining were adopted to study the organizational structure of B. tabaci. The slices were examined and photographed under fluorescence microscope. [ Result] The coloring of H-E staining cells was good. Under 400 x conditions, the fat body, compound eyes, nucleus and muscle of B. tabaci were clear. [ Conclusion] The production method for paraffin section of B. tabaci was reliable, and the quality of slices was high.展开更多
Gene amplification is a common mechanism of oncogene activation and contributes to tumor progression. Analysis of such genetic alterations are relevant to the understanding of tumor genetics and could provide prognost...Gene amplification is a common mechanism of oncogene activation and contributes to tumor progression. Analysis of such genetic alterations are relevant to the understanding of tumor genetics and could provide prognostic information for the individual patient. Standard analytical approaches using Southern blot and slot blot require a large amount of good展开更多
基金Supported by China Agriculture Research System(CARS-38)~~
文摘[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and a new method termed＆quot;opening a window on cornea and refixation＆quot; was used to prepare paraffin sections for cattle eyebal s in the treatment group. [Result] After the prepared specimens in the treatment group were fixed, it could be macroscopical y observed that retina and choroid were closely connected, with detachment occurring at a smal portion be-tween the two. According to the paraffin sections, it was microscopical y observed that the continuity of trabecular meshwork was intact, as wel as the continuity be-tween different layers of eyebal wal , without detachment between them, no retinal detachment, no shrinkage of each layer of tissue cells. [Conclusion] This study pro-vides a foundation for the basic research and pathological study of eyebal s.
基金funded by Talents Introduction Plan of Yunnan Province-"High-End Foreign Experts"Program(Grant No.000019)。
文摘Chimonanthus praecox is an important ornamental plant and cut flower material in China.It blooms in the freezing winter and its flower emits charming fragrance.However,in different region the flowering time is variable.In order to understand the flowering mechanism of Ch.praecox in the winter,we studied the flower bud differentiation in Spring City-Kunming using paraffin sectioning method in the present study.Meanwhile we compared the differentiation process difference from different regions.It was found that the temperature is the key factor for its flower bud differentiation and blossom of Ch.praecox.In the process of bud differentiation,the temperature 20℃was the optimum for inducing changes from vegetative axillary buds to reproductive buds and subsequent morphological differentiation in Ch.praecox.Furthermore in the first three differentiation periods—tepal primordial stage,staminal primordial stage and pistil primordial stage,Kunming took the shortest time to finish the process due to very rapid temperature rise to 20℃,whereas,in Zhengzhou the time for these differentiations was the longest,which may be caused by the slow temperature rise.After May,the high temperature stress forced the flower buds into the first long dormant period in all regions except Kunming.In Kunming,the average temperature was only 20–25℃,so the flower bud continued to differentiate.In all regions,Kunming is the first to complete whole flower bud differentiation even on the early August,and started the second dormancy very early but very long.In the other regions,the plants went through a shorter dormancy and the low temperature broke the dormancy rapidly.Contrarily the plants of Kunming spent a longer period for the low temperature.Thus,the low temperature less than 10℃is a key factor to breaking the second dormancy.Surely the regular effects of temperature on flower bud differentiation and blossom is very helpful for florescence regulation of Ch.praecox.
基金Supported by the 2012 Innovation and Business Start-up Training Program for the National College Students of Jilin Agricultural University(2012093025)~~
文摘[Objective] Taking Pinus sylvestris needles as materials, a set of test con-ditions suitable for the preparation of paraffin sections of Pinus sylvestris needles was determined. [Method] Based on the traditional method for paraffin sections preparation, steps including fixation, dehydration, adhering to slides and staining were investigated taking the structural characteristics of pine needles into considera-tion. [Result] 70% ethanol was used in the FAA fixative; before affixed on slides, the cut sections were first expanded in a 40 ℃ water bath and taken out by using Su-perfrost Plus slides, fol owed by drying in a 35 ℃ oven for 24 h; pine needles were cut into 8 μm thick sections; staining was achieved by immersion in eosin for 5 min and in hematoxylin for 1 min. [Conclusion] The conditions obtained above can en-hance the effect of fixation and dehydration; adhering to slides is easy to operate and the sections are not easy to drop; the dyeing effect is relatively preferable.
基金Supported by National Natural Science Foundation of China ( NSFC )( 30970438,31071708)
文摘[Objective] The purpose was to introduce a preparation method for paraffin section of Bemisia tabaci, so as to lay the foundation for the studies on changes of organizational structure of B. tabaci. [ Method] The technique of paraffin section and the method of H-E staining were adopted to study the organizational structure of B. tabaci. The slices were examined and photographed under fluorescence microscope. [ Result] The coloring of H-E staining cells was good. Under 400 x conditions, the fat body, compound eyes, nucleus and muscle of B. tabaci were clear. [ Conclusion] The production method for paraffin section of B. tabaci was reliable, and the quality of slices was high.
文摘Gene amplification is a common mechanism of oncogene activation and contributes to tumor progression. Analysis of such genetic alterations are relevant to the understanding of tumor genetics and could provide prognostic information for the individual patient. Standard analytical approaches using Southern blot and slot blot require a large amount of good
