More recently,the concepts of cell-free supernatants(CFS,so-called postbiotics)and whole-cell postbiotics(WCP)of probiotics have gained increasing attention for the development of bio-based films.However,reports on CF...More recently,the concepts of cell-free supernatants(CFS,so-called postbiotics)and whole-cell postbiotics(WCP)of probiotics have gained increasing attention for the development of bio-based films.However,reports on CFS and WCP of Lactobacillus gasseri remain scarce.In this study,pea protein isolate/psyllium mucilage was incorporated with CFS and WCP of L.gasseri to produce bio-based edible films.pH,total soluble solid content,antioxidant activity,total phenolic content,antimicrobial activity,and structural analyses were conducted to characterize the CFS and WCP of L.gasseri.Furthermore,the effects of CFS(10%and 20%,v/v)and WCP(10%and 20%,v/v)on the physicochemical,mechanical,barrier,optical,microstructural,and antimicrobial prop-erties of the bio-based films were investigated.The results showed that CFS and WCP exhibited antioxidant and antibacterial activities and were significant sources of phenolic compounds.The incorporation of 20%WCP caused a significant increase in the thickness and water vapor permeability of films,while a significant increase in moisture content was observed with 10%WCP.The control and 10%WCP films had the highest tensile strength values.Furthermore,the addition of 10%WCP resulted in a significant increase in elasticity.Films incorporating CFS and WCP exhibited lower opacity values and darker,yellow,and red color intensities.The addition of CFS and WCP caused a rough texture and cracked structure on the surface of the film.In conclusion,pea protein/psyllium mucilage-based films containing CFS and WCP of L.gasseri can be considered as a new alternative for environmentally friendly bio-based films.展开更多
文摘More recently,the concepts of cell-free supernatants(CFS,so-called postbiotics)and whole-cell postbiotics(WCP)of probiotics have gained increasing attention for the development of bio-based films.However,reports on CFS and WCP of Lactobacillus gasseri remain scarce.In this study,pea protein isolate/psyllium mucilage was incorporated with CFS and WCP of L.gasseri to produce bio-based edible films.pH,total soluble solid content,antioxidant activity,total phenolic content,antimicrobial activity,and structural analyses were conducted to characterize the CFS and WCP of L.gasseri.Furthermore,the effects of CFS(10%and 20%,v/v)and WCP(10%and 20%,v/v)on the physicochemical,mechanical,barrier,optical,microstructural,and antimicrobial prop-erties of the bio-based films were investigated.The results showed that CFS and WCP exhibited antioxidant and antibacterial activities and were significant sources of phenolic compounds.The incorporation of 20%WCP caused a significant increase in the thickness and water vapor permeability of films,while a significant increase in moisture content was observed with 10%WCP.The control and 10%WCP films had the highest tensile strength values.Furthermore,the addition of 10%WCP resulted in a significant increase in elasticity.Films incorporating CFS and WCP exhibited lower opacity values and darker,yellow,and red color intensities.The addition of CFS and WCP caused a rough texture and cracked structure on the surface of the film.In conclusion,pea protein/psyllium mucilage-based films containing CFS and WCP of L.gasseri can be considered as a new alternative for environmentally friendly bio-based films.
