The Tibetan antelope(Pantholops hodgsonii),blue sheep(Pseudois nayaur),and Tibetan sheep(Ovis aries)are the dominant small ruminants in the Three-River-Source National Park(TRSNP).However,knowledge about the associati...The Tibetan antelope(Pantholops hodgsonii),blue sheep(Pseudois nayaur),and Tibetan sheep(Ovis aries)are the dominant small ruminants in the Three-River-Source National Park(TRSNP).However,knowledge about the association between gut microbiota and host adaptability remains poorly understood.Herein,multi-omics sequencing approaches were employed to investigate the gut microbiota-mediated forage adaption in these ruminants.The results revealed that although wild ruminants(WR)of P.hodgsoni and P.nayaur were faced with severe foraging environments with significantly low vegetation coverage and nutrition,the apparent forage digestibility of dry matter,crude protein,and acid detergent fiber was significantly higher than that of O.aries.The 16s rRNA sequencing showed that the gut microbiota in WR underwent convergent evolution,and alpha diversity in these two groups was significantly higher than that in O.aries.Moreover,indicator species,including Bacteroidetes and Firmicutes,exhibited positive relationships with apparent forage digestibility,and their relative abundances were enriched in the gut of WR.Enterotype analysis further revealed that enterotype 1 belonged to WR,and the abundance of fatty acid synthesis metabolic pathway-related enzyme genes was significantly higher than enterotype 2,represented by O.aries.Besides,the metagenomic analysis identified 14 pathogenic bacterial species,among which 10 potentially pathogenic bacteria were significantly enriched in the gut microbiota of O.aries.Furthermore,the cellulolytic strains and genes encoding cellulase and hemicellulase were significantly enriched in WR.In conclusion,our results provide new evidence of gut microbiota to facilitate wildlife adaption in severe foraging environments of the TRSNP,China.展开更多
Background:The Tibetan antelope(Pantholops hodgsonii)is a migratory ungulate species that inhabits the Qinghai-Tibet Plateau.In recent years,the population of Tibetan antelope has steadily increased owing to rigorous ...Background:The Tibetan antelope(Pantholops hodgsonii)is a migratory ungulate species that inhabits the Qinghai-Tibet Plateau.In recent years,the population of Tibetan antelope has steadily increased owing to rigorous protection measures to safeguard the species from commercial poaching.Currently,infrastructure development,competition with domestic livestock,and predation are the principal factors threatening the survival of Tibetan antelope.Result:Our study found that migratory Tibetan antelope can adapt to the development of infrastructure over time,decreasing the potential negative impacts of such developments.Conclusion:We,therefore,urge infrastructure development companies to incorporate wildlife corridors that enable free movement of wildlife populations,particularly for migratory species.展开更多
Background:The Tibetan antelope(Pantholops hodgsonii)is a migratory ungulate species that inhabits the Qinghai-Tibet Plateau.In recent years,the population of Tibetan antelope has steadily increased owing to rigorous ...Background:The Tibetan antelope(Pantholops hodgsonii)is a migratory ungulate species that inhabits the Qinghai-Tibet Plateau.In recent years,the population of Tibetan antelope has steadily increased owing to rigorous protection measures to safeguard the species from commercial poaching.Currently,infrastructure development,competition with domestic livestock,and predation are the principal factors threatening the survival of Tibetan antelope.Result:Our study found that migratory Tibetan antelope can adapt to the development of infrastructure over time,decreasing the potential negative impacts of such developments.Conclusion:We,therefore,urge infrastructure development companies to incorporate wildlife corridors that enable free movement of wildlife populations,particularly for migratory species.展开更多
基金supported by the Youth Fund Project of the National Natural Science Foundation of China(32100100)the Joint Grant from the Chinese Academy of Sciences-People’s Government of Qinghai Province on Sanjiangyuan National Park(LHZX-2022-02)+1 种基金the Youth Innovation Promotion Association,Chinese Academy of Sciencesthe National Science Foundation of Qinghai Province(2022-ZJ-943Q).
文摘The Tibetan antelope(Pantholops hodgsonii),blue sheep(Pseudois nayaur),and Tibetan sheep(Ovis aries)are the dominant small ruminants in the Three-River-Source National Park(TRSNP).However,knowledge about the association between gut microbiota and host adaptability remains poorly understood.Herein,multi-omics sequencing approaches were employed to investigate the gut microbiota-mediated forage adaption in these ruminants.The results revealed that although wild ruminants(WR)of P.hodgsoni and P.nayaur were faced with severe foraging environments with significantly low vegetation coverage and nutrition,the apparent forage digestibility of dry matter,crude protein,and acid detergent fiber was significantly higher than that of O.aries.The 16s rRNA sequencing showed that the gut microbiota in WR underwent convergent evolution,and alpha diversity in these two groups was significantly higher than that in O.aries.Moreover,indicator species,including Bacteroidetes and Firmicutes,exhibited positive relationships with apparent forage digestibility,and their relative abundances were enriched in the gut of WR.Enterotype analysis further revealed that enterotype 1 belonged to WR,and the abundance of fatty acid synthesis metabolic pathway-related enzyme genes was significantly higher than enterotype 2,represented by O.aries.Besides,the metagenomic analysis identified 14 pathogenic bacterial species,among which 10 potentially pathogenic bacteria were significantly enriched in the gut microbiota of O.aries.Furthermore,the cellulolytic strains and genes encoding cellulase and hemicellulase were significantly enriched in WR.In conclusion,our results provide new evidence of gut microbiota to facilitate wildlife adaption in severe foraging environments of the TRSNP,China.
基金This work was supported by the Science and Technology Department of Qinghai Province[2017-ZJ-724]the Ministry of Science and Technology of the People’s Republic of China[2019QZKK040104].
文摘Background:The Tibetan antelope(Pantholops hodgsonii)is a migratory ungulate species that inhabits the Qinghai-Tibet Plateau.In recent years,the population of Tibetan antelope has steadily increased owing to rigorous protection measures to safeguard the species from commercial poaching.Currently,infrastructure development,competition with domestic livestock,and predation are the principal factors threatening the survival of Tibetan antelope.Result:Our study found that migratory Tibetan antelope can adapt to the development of infrastructure over time,decreasing the potential negative impacts of such developments.Conclusion:We,therefore,urge infrastructure development companies to incorporate wildlife corridors that enable free movement of wildlife populations,particularly for migratory species.
基金supported by the Science and Technology Department of Qinghai Province[2017-ZJ-724]the Ministry of Science and Technology of the People's Republic of China[2019QZKK040104]
文摘Background:The Tibetan antelope(Pantholops hodgsonii)is a migratory ungulate species that inhabits the Qinghai-Tibet Plateau.In recent years,the population of Tibetan antelope has steadily increased owing to rigorous protection measures to safeguard the species from commercial poaching.Currently,infrastructure development,competition with domestic livestock,and predation are the principal factors threatening the survival of Tibetan antelope.Result:Our study found that migratory Tibetan antelope can adapt to the development of infrastructure over time,decreasing the potential negative impacts of such developments.Conclusion:We,therefore,urge infrastructure development companies to incorporate wildlife corridors that enable free movement of wildlife populations,particularly for migratory species.
