The study was conducted to identify Aeromonas spp.and Vibrio spp.from fresh Pangasius fish(n=153)in Cambodia and test their antimicrobial susceptibility to antibiotics.The samples were collected from different wet mar...The study was conducted to identify Aeromonas spp.and Vibrio spp.from fresh Pangasius fish(n=153)in Cambodia and test their antimicrobial susceptibility to antibiotics.The samples were collected from different wet markets of Phnom Penh city and Kampong Thom,and Siem Reap provinces.The bacteria were isolated by using selective medium and their AMR(Antimicrobial Resistance)profile was tested by API 20E technique,respectively.Susceptibility profile was determined for seven antibiotics commonly used.The Vibrio spp.(34.64%,n=53)was found to be higher than Aeromonas spp.(24.83%,n=38).Four Vibrio and four Aeromonas species were identified where V.parahaemolyticus(57%,n=30)was the highest,followed by V.cholerae(38%,n=20),V.fluvialis(3.8%,n=2)and V.aglinolyticus(1.9%,n=1),whereas A.hydrophila(47%,n=18)was the highest,followed by A.hydrophila/caviae(45%,n=17),A.sobria(5%,n=2),A.caviae(2.6%,n=1).All the species presented high multi-resistance to the tested antibiotics.The antibiotic susceptibility profile to ampicillin(74%-100%),ciprofloxacin(7%-100%),sulfamethoxazole/trimethoprim(14%-100%),florfenicol(14%-100%),oxytetracycline(7%-100%),erythromycin(10%-100%)and colistin sulphate(33%-100%)was revealed resistance level in Aeromonas spp.whereas few species of Vibrio spp.resistant to ampicillin(43%-100%),ciprofloxacin(14%-100%),sulfamethoxazole/trimethoprim(14%-100%),florfenicol(14%-100%),oxytetracycline(20%-100%),erythromycin(29%-100%),colistin sulphate(33%-100%)were also identified.The results revealed these Aeromonas spp.and Vibrio spp.are potentially reservoirs of antibiotic resistance genes.MDR(Multidrug Resistance)was widespread among the samples isolated.That is a high-risk source of contamination since those pathogens and antimicrobials are often used.Our findings highlight that the aquatic environment and fresh Pangasius fish act as reservoirs of AMR Aeromonas spp.and Vibrio spp.which underline the need for a judicious use of antimicrobials and timely surveillance of AMR in aquaculture.Overall,the findings of our study indicated the presence of A.hydrophila,A.hydrophila/caviae,A.caviae,A.sobria,V.parahaemolyticus,V.cholerae,V.alginolyticus and V.fluvialis and high MDR.This result will allow us to identify the potential risk over circulating isolates in animal health and public health and the spread through the food chain offering supports for appropriate sanitary actions.展开更多
为改善巴沙鱼糜品质,将金花茶多糖(Camellia nitidissima Chi polysaccharide,CNCP)加入巴沙鱼糜,通过两段加热法制成CNCP-巴沙鱼糜复合凝胶。考察不同CNCP添加量(0%、0.2%、0.4%、0.6%、0.8%、1.0%)对CNCP-巴沙鱼糜复合凝胶的白度值、...为改善巴沙鱼糜品质,将金花茶多糖(Camellia nitidissima Chi polysaccharide,CNCP)加入巴沙鱼糜,通过两段加热法制成CNCP-巴沙鱼糜复合凝胶。考察不同CNCP添加量(0%、0.2%、0.4%、0.6%、0.8%、1.0%)对CNCP-巴沙鱼糜复合凝胶的白度值、凝胶强度、质构特性、感官评价、蒸煮损失率、持水性、水分分布、动态流变学特性、凝胶形成作用力、蛋白质二级结构、微观结构的影响。结果表明:随着CNCP添加量的增多,凝胶性能呈先升后降的趋势,并在添加量为0.4%时达到峰值。此时,鱼糜凝胶强度为202.59 g·cm,硬度、感官评分、持水性相比对照组分别提升20.1%、35.02%、23.2%,具有最高的储能模量(G′),自由水含量仅为2.3%,不易流动水的弛豫时间显著缩短,蒸煮损失率显著降低,二硫键含量显著增加(P<0.05),α-螺旋向更稳定的β-折叠结构转变,此时微观结构孔隙最小,分形维数最大,凝胶网状结构最为致密。综上所述,CNCP的添加有助于与巴沙鱼糜形成致密的复合凝胶网络,从而改善鱼糜凝胶品质,为进一步开发优质健康的鱼糜产品提供新的思路。展开更多
[ Objective] The aim of this study was to determine the effects of dietary phospholipids on Na＋ - K＋ - ATPase activity and cell mem- brane fatty acid composition in the gill of catfish （Pangasius sutchi）. [Method]...[ Objective] The aim of this study was to determine the effects of dietary phospholipids on Na＋ - K＋ - ATPase activity and cell mem- brane fatty acid composition in the gill of catfish （Pangasius sutchi）. [Method] 900 healthy catfish （1.4 ±0.08 g of average weight） were randomly divided into five groups. Dietary phospholipids level of group PL0 was 0%, and 1%, 2%, 3%, 4% for group PL1, PL2, PL3, PL4, respectively. The experiment lasted for 56 days. [Result] The results showed that the Na＋ -K＋ -ATPase activity in gill decreased gradually with the increase of the dietary phospholipids level （ P 〈0.05）. Cell membrane fatty acid composition was affected significantly by dietary phospholipids. The content of saturated fatty acids and monounsaturated fatty acids of group PLO was the highest （ P 〈 0.05）, however, it had the lowest content of highlyunsatu- rated fatty acids （ P 〈 0.05）. The content of polyunsaturated fatty acids of group 3 was higher than group 0 and group 1 （ P 〈 0.05）. E Conclusion] The results indicated that there is obvious compensation effects on Na＋ -K＋ -ATPase activity with phospholipids content changes in gills of catfish （ Pangasius sutchi） to maintain stable physiological level and normal basal metabolism and the increase of HUFA and ∑PUFA is necessary for gill cell membrane to better maintain and perform normal physiological function.展开更多
Snail eater pangasius （Pangasius conchophilus） is a freshwater fish species indigenous to Mekong-Chaophraya Basin, and is an economic fish in Thailand. This paper reviews some Thai research papers for referable bene...Snail eater pangasius （Pangasius conchophilus） is a freshwater fish species indigenous to Mekong-Chaophraya Basin, and is an economic fish in Thailand. This paper reviews some Thai research papers for referable benefit of international countries. The relationships of fecundity with body length and weight of wild population are related （F = 0.54L3339, F = 435.41W^S399）. Length-weight relationships of both sexes are W = 0.0059L3044s. Sex ratio is 1：1 and spawning season starts during March to June, the egg is adhesive demersal. Average mature size of both sexes are from 40-60 cm TL and 1,100-2,900 g. Effect of 3 types of hormone on induced ovulation, including HCG, pituitary gland, and buserelin in combination with domperidone are not signiticantly different （P 〉 0.05）. Hatching occurs in 24-25 hours after fertilization. The hatching percentage is 80%. Nursing and feeding strategies are also reviewed in this paper.展开更多
基金supported by grants from HEIP(Higher Education Improvement Project)Project and Royal University of Agriculture.
文摘The study was conducted to identify Aeromonas spp.and Vibrio spp.from fresh Pangasius fish(n=153)in Cambodia and test their antimicrobial susceptibility to antibiotics.The samples were collected from different wet markets of Phnom Penh city and Kampong Thom,and Siem Reap provinces.The bacteria were isolated by using selective medium and their AMR(Antimicrobial Resistance)profile was tested by API 20E technique,respectively.Susceptibility profile was determined for seven antibiotics commonly used.The Vibrio spp.(34.64%,n=53)was found to be higher than Aeromonas spp.(24.83%,n=38).Four Vibrio and four Aeromonas species were identified where V.parahaemolyticus(57%,n=30)was the highest,followed by V.cholerae(38%,n=20),V.fluvialis(3.8%,n=2)and V.aglinolyticus(1.9%,n=1),whereas A.hydrophila(47%,n=18)was the highest,followed by A.hydrophila/caviae(45%,n=17),A.sobria(5%,n=2),A.caviae(2.6%,n=1).All the species presented high multi-resistance to the tested antibiotics.The antibiotic susceptibility profile to ampicillin(74%-100%),ciprofloxacin(7%-100%),sulfamethoxazole/trimethoprim(14%-100%),florfenicol(14%-100%),oxytetracycline(7%-100%),erythromycin(10%-100%)and colistin sulphate(33%-100%)was revealed resistance level in Aeromonas spp.whereas few species of Vibrio spp.resistant to ampicillin(43%-100%),ciprofloxacin(14%-100%),sulfamethoxazole/trimethoprim(14%-100%),florfenicol(14%-100%),oxytetracycline(20%-100%),erythromycin(29%-100%),colistin sulphate(33%-100%)were also identified.The results revealed these Aeromonas spp.and Vibrio spp.are potentially reservoirs of antibiotic resistance genes.MDR(Multidrug Resistance)was widespread among the samples isolated.That is a high-risk source of contamination since those pathogens and antimicrobials are often used.Our findings highlight that the aquatic environment and fresh Pangasius fish act as reservoirs of AMR Aeromonas spp.and Vibrio spp.which underline the need for a judicious use of antimicrobials and timely surveillance of AMR in aquaculture.Overall,the findings of our study indicated the presence of A.hydrophila,A.hydrophila/caviae,A.caviae,A.sobria,V.parahaemolyticus,V.cholerae,V.alginolyticus and V.fluvialis and high MDR.This result will allow us to identify the potential risk over circulating isolates in animal health and public health and the spread through the food chain offering supports for appropriate sanitary actions.
文摘为改善巴沙鱼糜品质,将金花茶多糖(Camellia nitidissima Chi polysaccharide,CNCP)加入巴沙鱼糜,通过两段加热法制成CNCP-巴沙鱼糜复合凝胶。考察不同CNCP添加量(0%、0.2%、0.4%、0.6%、0.8%、1.0%)对CNCP-巴沙鱼糜复合凝胶的白度值、凝胶强度、质构特性、感官评价、蒸煮损失率、持水性、水分分布、动态流变学特性、凝胶形成作用力、蛋白质二级结构、微观结构的影响。结果表明:随着CNCP添加量的增多,凝胶性能呈先升后降的趋势,并在添加量为0.4%时达到峰值。此时,鱼糜凝胶强度为202.59 g·cm,硬度、感官评分、持水性相比对照组分别提升20.1%、35.02%、23.2%,具有最高的储能模量(G′),自由水含量仅为2.3%,不易流动水的弛豫时间显著缩短,蒸煮损失率显著降低,二硫键含量显著增加(P<0.05),α-螺旋向更稳定的β-折叠结构转变,此时微观结构孔隙最小,分形维数最大,凝胶网状结构最为致密。综上所述,CNCP的添加有助于与巴沙鱼糜形成致密的复合凝胶网络,从而改善鱼糜凝胶品质,为进一步开发优质健康的鱼糜产品提供新的思路。
基金supported by Guangxi Natural Science Foundation(2012GXNSFBA053053)The"Twelfth Five-Year"National Science and Technology Support Program ( 2012BAD25B04)Guangxi University"Student Innovation Training Program"(201152)
文摘[ Objective] The aim of this study was to determine the effects of dietary phospholipids on Na＋ - K＋ - ATPase activity and cell mem- brane fatty acid composition in the gill of catfish （Pangasius sutchi）. [Method] 900 healthy catfish （1.4 ±0.08 g of average weight） were randomly divided into five groups. Dietary phospholipids level of group PL0 was 0%, and 1%, 2%, 3%, 4% for group PL1, PL2, PL3, PL4, respectively. The experiment lasted for 56 days. [Result] The results showed that the Na＋ -K＋ -ATPase activity in gill decreased gradually with the increase of the dietary phospholipids level （ P 〈0.05）. Cell membrane fatty acid composition was affected significantly by dietary phospholipids. The content of saturated fatty acids and monounsaturated fatty acids of group PLO was the highest （ P 〈 0.05）, however, it had the lowest content of highlyunsatu- rated fatty acids （ P 〈 0.05）. The content of polyunsaturated fatty acids of group 3 was higher than group 0 and group 1 （ P 〈 0.05）. E Conclusion] The results indicated that there is obvious compensation effects on Na＋ -K＋ -ATPase activity with phospholipids content changes in gills of catfish （ Pangasius sutchi） to maintain stable physiological level and normal basal metabolism and the increase of HUFA and ∑PUFA is necessary for gill cell membrane to better maintain and perform normal physiological function.
文摘Snail eater pangasius （Pangasius conchophilus） is a freshwater fish species indigenous to Mekong-Chaophraya Basin, and is an economic fish in Thailand. This paper reviews some Thai research papers for referable benefit of international countries. The relationships of fecundity with body length and weight of wild population are related （F = 0.54L3339, F = 435.41W^S399）. Length-weight relationships of both sexes are W = 0.0059L3044s. Sex ratio is 1：1 and spawning season starts during March to June, the egg is adhesive demersal. Average mature size of both sexes are from 40-60 cm TL and 1,100-2,900 g. Effect of 3 types of hormone on induced ovulation, including HCG, pituitary gland, and buserelin in combination with domperidone are not signiticantly different （P 〉 0.05）. Hatching occurs in 24-25 hours after fertilization. The hatching percentage is 80%. Nursing and feeding strategies are also reviewed in this paper.
