Pampus argenteus is a commercially valuable species in many countries.Its artificial cultivation has been impacted by intestinal flatulence,leading to increased mortality.However,the role of gut microbiota in this pro...Pampus argenteus is a commercially valuable species in many countries.Its artificial cultivation has been impacted by intestinal flatulence,leading to increased mortality.However,the role of gut microbiota in this process remains unclear.Therefore,this study utilized high-throughput sequencing of 16S rRNA genes to investigate the gut microbiota in both flatulent and healthy P.argenteus.A total of 227571 high-quality and classifiable reads,representing 312 Amplicon Sequence Variants(ASVs),were obtained.Proteobacteria emerged as the predominant phylum in all samples,with the flatulent group displaying the highest relative abundance(85.3%).Microbial diversity was significantly greater in samples from water than those from tissues.Composition analysis using Bray-Curtis distance via CPCoA revealed distinct gut microbiota compositions between the samples with flatulence and the healthy samples.Co-occurrence network analysis demonstrated that the bacterial community in the flatulent group exhibited disorder due to the high abundance of Vibrio.Functional predictive analysis(PICRUSt2)indicated that flatulence in P.argenteus was associated with impaired methane and lipid metabolism,potentially resulting in excessive methane synthesis utilizing hydrogen and acetic acid as substrates produced by Clostridium sensu stricto 1,Cetobacterium,and Peptoclostridium.This study holds significant implications for early diagnosis and prevention of intestinal flatulence through the regulation of gut microbiota.展开更多
Juvenile silver pomfret, Pampus argenteus, was grown in culture tanks for 9 weeks on four different diets, and their effects on fish growth, digestive enzyme activity, and body composition were assessed. The feeding r...Juvenile silver pomfret, Pampus argenteus, was grown in culture tanks for 9 weeks on four different diets, and their effects on fish growth, digestive enzyme activity, and body composition were assessed. The feeding regime was as follows: Diet 1: fish meat; Diet 2: fish meat+artificial feed; Diet 3: fish meat+artificial feed+Agamaki clam meat; Diet 4: fish meat+artificial feed+Agamaki clam+copepods. The greatest weight gain was associated with Diet 4, while the lowest weight gain was associated with Diet 1. No significant difference was observed in weight gain between fish receiving Diet 2 and Diet 3. Specific growth rate followed similar trends as weight gain. The feed conversion ratio (FCR) of fish fed Diet 1 was significantly higher than the other fish groups, but no significant differences were observed in FCRs of fish fed Diet 2, Diet 3 or Diet 4. There was also no significant difference in the hepatosomatic index (HSI) between the four diets. For fish that received Diets 2-4, containing artificial feed, higher protease activities were detected. A higher lipid content of the experimental diets also significantly increased lipase activities and body lipid content. No significant differences in amylase activity or body protein content were found between Diets 1-4. In conclusion, a variety of food components, including copepods and artificial feed, in the diet of silver pomfret significantly increased digestive enzyme activity and could improve growth performance.展开更多
Although Pampus minor has been classified as a new species, it still remains controversial. Was used a DNA barcoding technique based on homologous sequence analysis of the16S and CO1 genes to clarify the confusion ove...Although Pampus minor has been classified as a new species, it still remains controversial. Was used a DNA barcoding technique based on homologous sequence analysis of the16S and CO1 genes to clarify the confusion over the identification of this species. Among 12 individuals whose genetic distance was 0.002, two haplotypes were found. According to the 16S sequences, the genetic distances ranged from 0.121 to 0.133 between P. minor and other Pampus species. Although the same the genetic distance between the two P minor haplotypes was generated using CO1 sequences, the haplotype of Pm22-23, Pm28, and Pm32-33 was the same as that of Pci EF607462 and EF607466, while the haplotype of Pm24-27 and Pm29-31 was the same as that of Pci EF607461 and EF607463-65. In addition, the genetic distance ranged only from 0.002 to 0.005 between P minor and Pa EF607460 and EF607458. Apart from this, the interspecies genetic distances varied from 0.135 to 0.143 between P minor and other t＇ampus species according to the C01 sequences. Phylogenetic trees, using combined 16S and CO1 data, strongly support the viewpoint that all the P. minor individuals form one clade that is in a sister position to Pampus sp. individuals （EU357803, FJ434342-FJ434343, and FJ652423-FJ652427）.展开更多
The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±...The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±0.1 g actively preyed on both the species. Their daily A. aurita consumption was 11.6 times their own body weights regardless of the size of A. aurita medusae. Their daily R. esculent um consumption was 13, 9.1, 5, and 4.1 times their own body weights when the R. esculentum medusae were 10, 20, 30, and 40 mm in bell diameter, respectively. The survival rates of the R. esculent um were higher than those of the A. aurita. When the R. esculent um medusae were more than 30 mm in bell diameter, their survival rate exceeded 92%. Silver pomfrets serve as a type of potential predators on A. aurita in coastal waters, and they have little infl uence on R. esculent um with a size exceeding 30 mm. Besides, A. aurita may be able to be used as fi sh prey in silver pomfret artifi cial breeding.展开更多
The contamination of fish type products such as silver pomfret fish fillets by potentially toxic elements(PTEs)has raised global health concerns.Related studies regarding the concentration of PTEs in fillets of silver...The contamination of fish type products such as silver pomfret fish fillets by potentially toxic elements(PTEs)has raised global health concerns.Related studies regarding the concentration of PTEs in fillets of silver pomfret fish were retrieved among some international databases such as Scopus,Pub Med and Embase between 1 January 1983 and 10 March 2020.The pooled(mean)concentration of PTEs in fillets of silver pomfret fish was meta-analyzed with the aid of a random-effect model(REM).Also,the non-carcinogenic risk was estimated via calculating the 95 th percentile of the total target hazard quotient(TTHQ).The meta-analysis of 21 articles(containing 25 studies or data reports)indicated that the ranking of PTEs in fillets of silver pomfret fish was Fe(11,414.81μg/kg wet weight,ww)>Zn(6055.72μg/kg ww)>Cr(1825.79μg/kg ww)>Pb(1486.44μg/kg ww)>Se(1053.47μg/kg ww)>Cd(992.50μg/kg ww)>Ni(745.23μg/kg ww)>Cu(669.71μg/kg ww)>total As(408.24μg/kg ww)>Co(87.03μg/kg ww)>methyl Hg(46.58μg/kg ww).The rank order of health risk assessment by country based on the TTHQ for adult consumers was Malaysia(2.500)>Bangladesh(0.886)>Iran(0.144)>China(0.045)>Pakistan(0.020)>India(0.015),while the corresponding values for child consumers was Malaysia(11.790)>Bangladesh(4.146)>Iran(0.675)>China(0.206)>Pakistan(0.096)>India(0.077).The adult consumers in Malaysia and children in Malaysia and Bangladesh were at considerable non-carcinogenic risk.Therefore,following the recommended control plans in order to reduce the health risk associated with the ingestion of PTEs via consumption of silver pomfret fish fillets is crucial.展开更多
Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous...Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus argenteus.The qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then down.The expression levels of vgr in the testis were low and only 1%-3%of that in ovary.ELISA results show that during the ovarian development of P.argenteus,VTG in liver,serum,and ovary all showed a trend from increasing to decreasing.However,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA expression.The VGR content in the testis was extremely low and stable,consistent with vgr mRNA.Immunohistochemistry results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the oocyte.Therefore,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the development of P.argenteus was revealed preliminarily.展开更多
Pampus cinereus （Bloch, 1795） （Stromateidae）, a species believed to be widely distributed throughout the Indo-Western Pacific region, was redescribed and a neotype was designated. The designation of a neotype was ...Pampus cinereus （Bloch, 1795） （Stromateidae）, a species believed to be widely distributed throughout the Indo-Western Pacific region, was redescribed and a neotype was designated. The designation of a neotype was necessary because of ambiguous data in Bloch＇s original description and the loss of the original type specimen. Morphological data indicated that 10 recently-collected specimens from the coasts of southem China agreed well with Bloch＇s original description and figure ofP. cinereus. A neotype for this species was selected from among the 10 specimens, and a detailed description is presented in this paper.展开更多
Sagittal otoliths morphology were compared to identify five Pampus species of the Chinese coast（Chinese pomfret Pampus chinensis, grey pomfret P. cinereus, southern lesser pomfret P. minor, plumage pomfret P.punctati...Sagittal otoliths morphology were compared to identify five Pampus species of the Chinese coast（Chinese pomfret Pampus chinensis, grey pomfret P. cinereus, southern lesser pomfret P. minor, plumage pomfret P.punctatissmus, and Pampus sp.）. Otoliths demonstrated species-specific characteristics with noticeable differences in overall shape, margin and anterior region among species. With species divergence, otoliths get thinner following further developed rostrum and anti-rostrum. Interspecies variation in otolith could associate with experienced different temperature and water column. The discriminant analysis presented a high classification accuracy of 96.6%, which confirms the inter-specific distinction of otolith shape and enable the species identification.展开更多
Pampus minor is an important commercial fish.Due to the similarity of external morphological characteristics among the genus Pampus species,P.minor has often been identified as the juvenile group of both P.cinereus an...Pampus minor is an important commercial fish.Due to the similarity of external morphological characteristics among the genus Pampus species,P.minor has often been identified as the juvenile group of both P.cinereus and P.argenteus.While little genetic background on this species is known,this study was based on control region sequences and provided the first evaluation of the genetic signature of 264 individuals of P.minor from 11 populations along the coasts of China and Malaysia.The results indicate high genetic haplotype diversity and low nucleotide diversity in this species.Additionally,two differentiated haplotype lineages were identified in the P.minor populations.However,phylogenetic structures corresponding to the geographical locations were unable to be established.Analysis of molecular variance identified a vast majority of the genetic variation occurring within populations.F-statistic test value(FST)of pairwise indicated that great differences existed between the Chinese and Malaysian P.minor populations.For the Chinese populations,the genetic differences were insignificant with the exception of the Xiamen population,which is a marginal population.During the late Pleistocene,a population expansion of P.minor occurred.These expanded populations originated from the glacial refugium in the South China Sea and then rapidly occupied and adapted to their new habitat.The results of this study provide genetic information for ensuring the protection and management of P.minor resources.展开更多
Pampus punctatissimus (Temminck et Schlegel , 1845) and Pampus argenteus (Euphrasen , 1788) are two important species of the genus pampus and widely distributed in Chinese coastal waters . Their classification has bee...Pampus punctatissimus (Temminck et Schlegel , 1845) and Pampus argenteus (Euphrasen , 1788) are two important species of the genus pampus and widely distributed in Chinese coastal waters . Their classification has been controversial for many years . Due to the similarities of their overall appearance , many workers regarded P punctatissimus as a synonym of P argenteus Even though a few authors recognized the differences between them ,there has been great confusion on the nomenclature . Morpholoically , these two species have obvious differences in some aspects , in particular, the number of gill rakers and vertebrae, the lateral line branches in the head, the fin formula , etc.. As these differences comprised sufficient differentiation of these two species, Pampus punctatissimus (Temminck et Schlegel, 1845) was recognized as a species distinct from P argenteus (Euphrasen, 1788). A redescription of it is given in the present study.展开更多
Thirty-two 63 .0 - 134.4 mm standard length specimens collected during 1954 - 1990 from the coastal waters of the South China Sea and the continental coast of the Taiwan Strait were identified as a new species, Pampus...Thirty-two 63 .0 - 134.4 mm standard length specimens collected during 1954 - 1990 from the coastal waters of the South China Sea and the continental coast of the Taiwan Strait were identified as a new species, Pampus minor Liu et Li, sp. nov..展开更多
The morphological similarities of Pampus fishes have led to considerable confusion in species-level identification,and no accurate information on neotype or DNA barcoding of Pampus echinogaster is available. Two hundr...The morphological similarities of Pampus fishes have led to considerable confusion in species-level identification,and no accurate information on neotype or DNA barcoding of Pampus echinogaster is available. Two hundred and seven specimens of P. echinogaster were collected from the coastal waters of Dandong, Dongying, Qingdao,Nantong, Zhoushan, Wenzhou, Changle, Taiwan, and Wakayama（Japan）, from June 2010 to April 2013. The diagnostic characteristics of P. echinogaster are as follows： dorsal fin VIII-XI-43–51, anal fin V-VIII-43–49, pectoral fin 22–27, caudal fin 19–22, pelvic fin absent; first gill rakers sparse, slender（pointed）, 3–4＋12–16=15–20; vertebrae39–41; transverse occipital canal on top of head moderately small, wavy ridges not reaching upper origin of pectoral fin; ventral branch of lateral line canal spare, shorter than dorsal branch of lateral line canal. By combining congener sequences of the cytochrome oxidase I（COI） gene from Gen Bank, two absolute groups were detected among all specimens, which further indicated that two valid species were present based on genetic differences in amino acid sequences and the distance between the groups. The sequences of Group 1 can be regarded as DNA barcoding of P. echinogaster. The correct morphological redescription and DNA barcoding of P.echinogaster are presented here to provide a guarantee for efficient and accurate studies, a theoretical basis for classification, and enable appropriate fishery management and conservation strategies for the genus Pampus in the future.展开更多
The distribution of Pampus argenteus(Euphrasen,1788)spans a pronounced latitudinal-environmental gradient from the subtropical to the subpolar zones.The species is reported to have multiple stocks along coastal China,...The distribution of Pampus argenteus(Euphrasen,1788)spans a pronounced latitudinal-environmental gradient from the subtropical to the subpolar zones.The species is reported to have multiple stocks along coastal China,exhibiting different spawning behaviors and habitat preferences.Such ecological variations might imply potential genetic divergence and local adaptation.We resequenced 117 genomes from six coastal stocks of P.argenteus in China.Although no hierarchical genetic structure was identified,over 50%of the single-nucleotide polymorphisms(SNPs)indicated moderate to strong divergence in at least two stocks.The Mantel test identified 21100-kb sliding windows with significant isolation by distance and environ-ment,while a majority did not.Given the lack of genome-wide isolation by distance,the 21 windows may be under selec-tion pressure from the latitudinal-environmental variations.Among the 21 windows,certain genes were linked to circadian clock regulation and thermal stress response,suggesting sea surface temperature and sunshine duration as selective forces.A total of 17 genes regulated neuron activity;variations near these genes might subsequently shape the different spawning and migratory behaviors among the stocks.Additionally,1204 SNPs were mapped to non-coding regions;14 transcriptional and translational factors were identified in the 21 windows.These findings imply that alterations in gene expression might contribute to the local adaptation of the P.argenteus stocks.展开更多
基金funded by the National Key R&D Program of China(No.2022YFD2400100)the Ningbo 2025 Major Project of Science Technology and Innovation(No.2021Z003)+5 种基金the Public Welfare Program of Ningbo City(Science and Technology Special Commissioner Project)(No.2022S204)the National Natural Science Foundation of China(Nos.31872195,42076118 and 42306114)the China Postdoctoral Science Foundation(No.2022M721729)the General Scientific Research Projects of Zhejiang Provincial Department of Education(No.Y202249062)the Ningbo Public Welfare Science and Technology Project(No.2021S061)the Ningbo Yongjiang Talent Introduction Programme(No.2021B-029-C)。
文摘Pampus argenteus is a commercially valuable species in many countries.Its artificial cultivation has been impacted by intestinal flatulence,leading to increased mortality.However,the role of gut microbiota in this process remains unclear.Therefore,this study utilized high-throughput sequencing of 16S rRNA genes to investigate the gut microbiota in both flatulent and healthy P.argenteus.A total of 227571 high-quality and classifiable reads,representing 312 Amplicon Sequence Variants(ASVs),were obtained.Proteobacteria emerged as the predominant phylum in all samples,with the flatulent group displaying the highest relative abundance(85.3%).Microbial diversity was significantly greater in samples from water than those from tissues.Composition analysis using Bray-Curtis distance via CPCoA revealed distinct gut microbiota compositions between the samples with flatulence and the healthy samples.Co-occurrence network analysis demonstrated that the bacterial community in the flatulent group exhibited disorder due to the high abundance of Vibrio.Functional predictive analysis(PICRUSt2)indicated that flatulence in P.argenteus was associated with impaired methane and lipid metabolism,potentially resulting in excessive methane synthesis utilizing hydrogen and acetic acid as substrates produced by Clostridium sensu stricto 1,Cetobacterium,and Peptoclostridium.This study holds significant implications for early diagnosis and prevention of intestinal flatulence through the regulation of gut microbiota.
基金Supported by the National Key Technology Research and Development Program during the Twelfth Five-Year Plan Period(No.2011BAD13B01)the Central Nonprofit Basic Scientific Research Project for the Scientific Research Institutes of China(No.East-2011M09)the Research Project Commissioned by the Shanghai Agricultural Commission(No.2007-4-1)
文摘Juvenile silver pomfret, Pampus argenteus, was grown in culture tanks for 9 weeks on four different diets, and their effects on fish growth, digestive enzyme activity, and body composition were assessed. The feeding regime was as follows: Diet 1: fish meat; Diet 2: fish meat+artificial feed; Diet 3: fish meat+artificial feed+Agamaki clam meat; Diet 4: fish meat+artificial feed+Agamaki clam+copepods. The greatest weight gain was associated with Diet 4, while the lowest weight gain was associated with Diet 1. No significant difference was observed in weight gain between fish receiving Diet 2 and Diet 3. Specific growth rate followed similar trends as weight gain. The feed conversion ratio (FCR) of fish fed Diet 1 was significantly higher than the other fish groups, but no significant differences were observed in FCRs of fish fed Diet 2, Diet 3 or Diet 4. There was also no significant difference in the hepatosomatic index (HSI) between the four diets. For fish that received Diets 2-4, containing artificial feed, higher protease activities were detected. A higher lipid content of the experimental diets also significantly increased lipase activities and body lipid content. No significant differences in amylase activity or body protein content were found between Diets 1-4. In conclusion, a variety of food components, including copepods and artificial feed, in the diet of silver pomfret significantly increased digestive enzyme activity and could improve growth performance.
基金Supported by National Natural Science Foundation of China (No. 40676085)
文摘Although Pampus minor has been classified as a new species, it still remains controversial. Was used a DNA barcoding technique based on homologous sequence analysis of the16S and CO1 genes to clarify the confusion over the identification of this species. Among 12 individuals whose genetic distance was 0.002, two haplotypes were found. According to the 16S sequences, the genetic distances ranged from 0.121 to 0.133 between P. minor and other Pampus species. Although the same the genetic distance between the two P minor haplotypes was generated using CO1 sequences, the haplotype of Pm22-23, Pm28, and Pm32-33 was the same as that of Pci EF607462 and EF607466, while the haplotype of Pm24-27 and Pm29-31 was the same as that of Pci EF607461 and EF607463-65. In addition, the genetic distance ranged only from 0.002 to 0.005 between P minor and Pa EF607460 and EF607458. Apart from this, the interspecies genetic distances varied from 0.135 to 0.143 between P minor and other t＇ampus species according to the C01 sequences. Phylogenetic trees, using combined 16S and CO1 data, strongly support the viewpoint that all the P. minor individuals form one clade that is in a sister position to Pampus sp. individuals （EU357803, FJ434342-FJ434343, and FJ652423-FJ652427）.
基金Supported by National Basic Research Program of China(973 Program)(No.2011CB403605)the Key Science and Technology Program of Qingdao(No.11-3-1-6-hy)+1 种基金the Special Funds for the Basic R&D Programin the Central Non-Profit Research Institutes(No.20603022011008)the National Key Technology R&D Program during the Twelfth Five-Year Plan Period(No.2011BAD13B01)
文摘The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±0.1 g actively preyed on both the species. Their daily A. aurita consumption was 11.6 times their own body weights regardless of the size of A. aurita medusae. Their daily R. esculent um consumption was 13, 9.1, 5, and 4.1 times their own body weights when the R. esculentum medusae were 10, 20, 30, and 40 mm in bell diameter, respectively. The survival rates of the R. esculent um were higher than those of the A. aurita. When the R. esculent um medusae were more than 30 mm in bell diameter, their survival rate exceeded 92%. Silver pomfrets serve as a type of potential predators on A. aurita in coastal waters, and they have little infl uence on R. esculent um with a size exceeding 30 mm. Besides, A. aurita may be able to be used as fi sh prey in silver pomfret artifi cial breeding.
基金the student research committee at Shahid Beheshti University of Medical Sciences for the financial grants supporting this study(1398/9920)。
文摘The contamination of fish type products such as silver pomfret fish fillets by potentially toxic elements(PTEs)has raised global health concerns.Related studies regarding the concentration of PTEs in fillets of silver pomfret fish were retrieved among some international databases such as Scopus,Pub Med and Embase between 1 January 1983 and 10 March 2020.The pooled(mean)concentration of PTEs in fillets of silver pomfret fish was meta-analyzed with the aid of a random-effect model(REM).Also,the non-carcinogenic risk was estimated via calculating the 95 th percentile of the total target hazard quotient(TTHQ).The meta-analysis of 21 articles(containing 25 studies or data reports)indicated that the ranking of PTEs in fillets of silver pomfret fish was Fe(11,414.81μg/kg wet weight,ww)>Zn(6055.72μg/kg ww)>Cr(1825.79μg/kg ww)>Pb(1486.44μg/kg ww)>Se(1053.47μg/kg ww)>Cd(992.50μg/kg ww)>Ni(745.23μg/kg ww)>Cu(669.71μg/kg ww)>total As(408.24μg/kg ww)>Co(87.03μg/kg ww)>methyl Hg(46.58μg/kg ww).The rank order of health risk assessment by country based on the TTHQ for adult consumers was Malaysia(2.500)>Bangladesh(0.886)>Iran(0.144)>China(0.045)>Pakistan(0.020)>India(0.015),while the corresponding values for child consumers was Malaysia(11.790)>Bangladesh(4.146)>Iran(0.675)>China(0.206)>Pakistan(0.096)>India(0.077).The adult consumers in Malaysia and children in Malaysia and Bangladesh were at considerable non-carcinogenic risk.Therefore,following the recommended control plans in order to reduce the health risk associated with the ingestion of PTEs via consumption of silver pomfret fish fillets is crucial.
基金Supported by the National Natural Science Foundation of China(Nos.31872586,42076118)the Major Project of Science,Technology and Innovation 2025 in Ningbo City(No.2021Z003)the K.C.Wong Magna Fund in Ningbo University。
文摘Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus argenteus.The qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then down.The expression levels of vgr in the testis were low and only 1%-3%of that in ovary.ELISA results show that during the ovarian development of P.argenteus,VTG in liver,serum,and ovary all showed a trend from increasing to decreasing.However,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA expression.The VGR content in the testis was extremely low and stable,consistent with vgr mRNA.Immunohistochemistry results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the oocyte.Therefore,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the development of P.argenteus was revealed preliminarily.
基金Supported by the National Natural Science Foundation of China (Nos.31172053, 41276166)
文摘Pampus cinereus （Bloch, 1795） （Stromateidae）, a species believed to be widely distributed throughout the Indo-Western Pacific region, was redescribed and a neotype was designated. The designation of a neotype was necessary because of ambiguous data in Bloch＇s original description and the loss of the original type specimen. Morphological data indicated that 10 recently-collected specimens from the coasts of southem China agreed well with Bloch＇s original description and figure ofP. cinereus. A neotype for this species was selected from among the 10 specimens, and a detailed description is presented in this paper.
基金The Public Science and Technology Research Funds Projects of Ocean under contract No.201305030the Specialized Research Fund for the Doctoral Program of Higher Education under contract No.20120132130001the Fundamental Research Funds for the Central Universities under contract No.201262004
文摘Sagittal otoliths morphology were compared to identify five Pampus species of the Chinese coast（Chinese pomfret Pampus chinensis, grey pomfret P. cinereus, southern lesser pomfret P. minor, plumage pomfret P.punctatissmus, and Pampus sp.）. Otoliths demonstrated species-specific characteristics with noticeable differences in overall shape, margin and anterior region among species. With species divergence, otoliths get thinner following further developed rostrum and anti-rostrum. Interspecies variation in otolith could associate with experienced different temperature and water column. The discriminant analysis presented a high classification accuracy of 96.6%, which confirms the inter-specific distinction of otolith shape and enable the species identification.
基金The National Key Research and Development Program of China under contract No.2018YFC1406302the National Programme on Global Change and Air-Sea Interaction under contract No.GASI-02-SCS-YDsum+1 种基金the Fond of Fujian Provincial Research Institutes of Basic Research and Public Service Special Operations under contract No.2017R1006-5the Fond of Bilateral Cooperation of Maritime Affairs under contract No.2200207。
文摘Pampus minor is an important commercial fish.Due to the similarity of external morphological characteristics among the genus Pampus species,P.minor has often been identified as the juvenile group of both P.cinereus and P.argenteus.While little genetic background on this species is known,this study was based on control region sequences and provided the first evaluation of the genetic signature of 264 individuals of P.minor from 11 populations along the coasts of China and Malaysia.The results indicate high genetic haplotype diversity and low nucleotide diversity in this species.Additionally,two differentiated haplotype lineages were identified in the P.minor populations.However,phylogenetic structures corresponding to the geographical locations were unable to be established.Analysis of molecular variance identified a vast majority of the genetic variation occurring within populations.F-statistic test value(FST)of pairwise indicated that great differences existed between the Chinese and Malaysian P.minor populations.For the Chinese populations,the genetic differences were insignificant with the exception of the Xiamen population,which is a marginal population.During the late Pleistocene,a population expansion of P.minor occurred.These expanded populations originated from the glacial refugium in the South China Sea and then rapidly occupied and adapted to their new habitat.The results of this study provide genetic information for ensuring the protection and management of P.minor resources.
文摘Pampus punctatissimus (Temminck et Schlegel , 1845) and Pampus argenteus (Euphrasen , 1788) are two important species of the genus pampus and widely distributed in Chinese coastal waters . Their classification has been controversial for many years . Due to the similarities of their overall appearance , many workers regarded P punctatissimus as a synonym of P argenteus Even though a few authors recognized the differences between them ,there has been great confusion on the nomenclature . Morpholoically , these two species have obvious differences in some aspects , in particular, the number of gill rakers and vertebrae, the lateral line branches in the head, the fin formula , etc.. As these differences comprised sufficient differentiation of these two species, Pampus punctatissimus (Temminck et Schlegel, 1845) was recognized as a species distinct from P argenteus (Euphrasen, 1788). A redescription of it is given in the present study.
文摘Thirty-two 63 .0 - 134.4 mm standard length specimens collected during 1954 - 1990 from the coastal waters of the South China Sea and the continental coast of the Taiwan Strait were identified as a new species, Pampus minor Liu et Li, sp. nov..
基金The National Natural Science Foundation of China under contract No.41776171the National Programme on Global Change and Air-Sea Interaction under contract Nos GASI-02-SCS-YSWspr/aut and GASI-02-PAC-YDsum/aut+1 种基金the Scientific Research Foundation of TIO,SOA under contract No.2016010the Bilateral Cooperation of Maritime Affairs under contract No.2200207
文摘The morphological similarities of Pampus fishes have led to considerable confusion in species-level identification,and no accurate information on neotype or DNA barcoding of Pampus echinogaster is available. Two hundred and seven specimens of P. echinogaster were collected from the coastal waters of Dandong, Dongying, Qingdao,Nantong, Zhoushan, Wenzhou, Changle, Taiwan, and Wakayama（Japan）, from June 2010 to April 2013. The diagnostic characteristics of P. echinogaster are as follows： dorsal fin VIII-XI-43–51, anal fin V-VIII-43–49, pectoral fin 22–27, caudal fin 19–22, pelvic fin absent; first gill rakers sparse, slender（pointed）, 3–4＋12–16=15–20; vertebrae39–41; transverse occipital canal on top of head moderately small, wavy ridges not reaching upper origin of pectoral fin; ventral branch of lateral line canal spare, shorter than dorsal branch of lateral line canal. By combining congener sequences of the cytochrome oxidase I（COI） gene from Gen Bank, two absolute groups were detected among all specimens, which further indicated that two valid species were present based on genetic differences in amino acid sequences and the distance between the groups. The sequences of Group 1 can be regarded as DNA barcoding of P. echinogaster. The correct morphological redescription and DNA barcoding of P.echinogaster are presented here to provide a guarantee for efficient and accurate studies, a theoretical basis for classification, and enable appropriate fishery management and conservation strategies for the genus Pampus in the future.
基金supported by the National Natural Science Foundation of China(grant number 32270472)the Strategic Priority Research Program of the Chinese Academy of Sciences(grant number XDB42000000).
文摘The distribution of Pampus argenteus(Euphrasen,1788)spans a pronounced latitudinal-environmental gradient from the subtropical to the subpolar zones.The species is reported to have multiple stocks along coastal China,exhibiting different spawning behaviors and habitat preferences.Such ecological variations might imply potential genetic divergence and local adaptation.We resequenced 117 genomes from six coastal stocks of P.argenteus in China.Although no hierarchical genetic structure was identified,over 50%of the single-nucleotide polymorphisms(SNPs)indicated moderate to strong divergence in at least two stocks.The Mantel test identified 21100-kb sliding windows with significant isolation by distance and environ-ment,while a majority did not.Given the lack of genome-wide isolation by distance,the 21 windows may be under selec-tion pressure from the latitudinal-environmental variations.Among the 21 windows,certain genes were linked to circadian clock regulation and thermal stress response,suggesting sea surface temperature and sunshine duration as selective forces.A total of 17 genes regulated neuron activity;variations near these genes might subsequently shape the different spawning and migratory behaviors among the stocks.Additionally,1204 SNPs were mapped to non-coding regions;14 transcriptional and translational factors were identified in the 21 windows.These findings imply that alterations in gene expression might contribute to the local adaptation of the P.argenteus stocks.
