Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species f...Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species from the Gen Bank nucleotide database.The results of sequence comparisons showed the cox1 gene is 1 534 bp in length,including 326 varible sites and 211 parsimonious information sites.The percentage of A ＋T is 67.1% in the nucleotide sequences,showing a strong AT bias.Genetic distances among subfamilies are 0.08.Using Locusta migratoria as outgroup,the phylogenetic trees were reconstructed with NJ,MP,ML and Bayesian inferences,and the results showed that the clustering results were approximately identical to that of classical morphological classification.Thrinchinae and Pamphaginae were a monophyletic group,respectively.Two species of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae,classification position of Asiotmethis should be further discussed by both genetic markers and morphological features.The current genus Filchnerella of Prionotropisinae was not a monophyletic group.展开更多
Two new species of the genus Eotmethis Bey-Bienko, 1948 are described in this paper, namely E. daqingshanensis sp. nov. and E. gansuensis sp. nov. from the Inner Mongolia Autonomous Region and Gansu Province, respecti...Two new species of the genus Eotmethis Bey-Bienko, 1948 are described in this paper, namely E. daqingshanensis sp. nov. and E. gansuensis sp. nov. from the Inner Mongolia Autonomous Region and Gansu Province, respectively. The type specimens of the new species are deposited in College of Life Sciences, Hebei University, Baoding, China.展开更多
Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II(COIl)mtDNA sequences(684 bp).Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data,along with...Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II(COIl)mtDNA sequences(684 bp).Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data,along with four species from the GenBank nucleotide database.The purpose of this study was analyzing the phylogenetic relationships among subfamilies within Pamphagidae and interpreting the phylogenetic position of this family within the Acridoidea superfamily.Phylogenetic trees were reconstructed using neighbor-joining(NJ),maximurn parsimony(MP)and Bayesian inference(BI)methods.The 684 bp analyzed fragment included 126 parsimony informative sites.Sequences diverged 1.0%-11.1%between genera within subfamilies,and 8.8%-12.3%between subfamilies.Amino acid sequence diverged 0-6.1%between genera within subfamilies,and 0.4%-7.5%between subfamilies.Our phylogenetic trees revealed the monophyly of Pamphagidae and three distinct major groups within this family.Moreover,several well supported and stable clades were found in Pamphagidae.The global clustering results were similar to that obtained through classical morphological classification:Prionotropisinae,Thrinchinae and Pamphaginae were monophyletic groups.However,the current genus Filchnerella(Prionotropisinae)was not a monophyletic group and the genus Asiotmethis(Prionotropisinae)was a sister group of the genus Thrinchus(Thrinchinae).Further molec-ular and morphological studies are required to clarify the phylogenetic relationships of the genera Filchnerella and Asiotmethis.展开更多
基金Supported by National Natural Science Foundation of China(31272342)the 2ndGroup Innovative Foundation Project for Students in China in 2012(201210075004)~~
文摘Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species from the Gen Bank nucleotide database.The results of sequence comparisons showed the cox1 gene is 1 534 bp in length,including 326 varible sites and 211 parsimonious information sites.The percentage of A ＋T is 67.1% in the nucleotide sequences,showing a strong AT bias.Genetic distances among subfamilies are 0.08.Using Locusta migratoria as outgroup,the phylogenetic trees were reconstructed with NJ,MP,ML and Bayesian inferences,and the results showed that the clustering results were approximately identical to that of classical morphological classification.Thrinchinae and Pamphaginae were a monophyletic group,respectively.Two species of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae,classification position of Asiotmethis should be further discussed by both genetic markers and morphological features.The current genus Filchnerella of Prionotropisinae was not a monophyletic group.
基金supported by the National Natural Science Foundation of China(31372153)the Natural Foundation of Hebei Province(C2013201148)
文摘Two new species of the genus Eotmethis Bey-Bienko, 1948 are described in this paper, namely E. daqingshanensis sp. nov. and E. gansuensis sp. nov. from the Inner Mongolia Autonomous Region and Gansu Province, respectively. The type specimens of the new species are deposited in College of Life Sciences, Hebei University, Baoding, China.
文摘Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II(COIl)mtDNA sequences(684 bp).Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data,along with four species from the GenBank nucleotide database.The purpose of this study was analyzing the phylogenetic relationships among subfamilies within Pamphagidae and interpreting the phylogenetic position of this family within the Acridoidea superfamily.Phylogenetic trees were reconstructed using neighbor-joining(NJ),maximurn parsimony(MP)and Bayesian inference(BI)methods.The 684 bp analyzed fragment included 126 parsimony informative sites.Sequences diverged 1.0%-11.1%between genera within subfamilies,and 8.8%-12.3%between subfamilies.Amino acid sequence diverged 0-6.1%between genera within subfamilies,and 0.4%-7.5%between subfamilies.Our phylogenetic trees revealed the monophyly of Pamphagidae and three distinct major groups within this family.Moreover,several well supported and stable clades were found in Pamphagidae.The global clustering results were similar to that obtained through classical morphological classification:Prionotropisinae,Thrinchinae and Pamphaginae were monophyletic groups.However,the current genus Filchnerella(Prionotropisinae)was not a monophyletic group and the genus Asiotmethis(Prionotropisinae)was a sister group of the genus Thrinchus(Thrinchinae).Further molec-ular and morphological studies are required to clarify the phylogenetic relationships of the genera Filchnerella and Asiotmethis.
