Interest in the technology for producing alternative proteins is rapidly increasing,driven by the need to find new ways to produce and consume protein for the global population.This technology involves growing differe...Interest in the technology for producing alternative proteins is rapidly increasing,driven by the need to find new ways to produce and consume protein for the global population.This technology involves growing different microorganisms and animal cells under controlled conditions to ensure their viability and efficient growth.The cultivation process takes place in different types of bioreactors,from traditional models to innovative new designs,each offering unique features and capabilities.The most commonly used bioreactors are stirred tank reactors,which are mechanically agitated,and airlift or bubble column bioreactors,which are pneumatically agitated.These bioreactors are often adapted or modified to optimize the production of cultured meat.Essential to the process are microcarriers or scaffolds that support cell adhesion and proliferation.Other bioreactor models,such as hollow fiber and packed bed bioreactors,are also being explored.The trend towards single-use technology is growing due to benefits like easier cleaning and sterilization,and reduced operation times,though it does raise concerns about plastic waste.This review not only describes various bioreactor models but also discusses instrumentation and control systems.It aims to present the main bioreactor models currently in use for cultivated meat production,detailing their features,advantages,disadvantages,and the technological challenges that need to be addressed.展开更多
Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more effic...Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today＇s 2-dimensional（2D） standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef.展开更多
基金Conselho Nacional de Desenvolvimento Científico e Tecnológico,Process 440080/2022-3Luciana Porto de Souza Vandenberghe,409337/2023-4Luciana Porto de Souza Vandenberghe,Coordenação de Aperfeiçoamento de Pessoal de Nível Superior,CAPES-PROEX,Ariane Fátima Murawski de Mello。
文摘Interest in the technology for producing alternative proteins is rapidly increasing,driven by the need to find new ways to produce and consume protein for the global population.This technology involves growing different microorganisms and animal cells under controlled conditions to ensure their viability and efficient growth.The cultivation process takes place in different types of bioreactors,from traditional models to innovative new designs,each offering unique features and capabilities.The most commonly used bioreactors are stirred tank reactors,which are mechanically agitated,and airlift or bubble column bioreactors,which are pneumatically agitated.These bioreactors are often adapted or modified to optimize the production of cultured meat.Essential to the process are microcarriers or scaffolds that support cell adhesion and proliferation.Other bioreactor models,such as hollow fiber and packed bed bioreactors,are also being explored.The trend towards single-use technology is growing due to benefits like easier cleaning and sterilization,and reduced operation times,though it does raise concerns about plastic waste.This review not only describes various bioreactor models but also discusses instrumentation and control systems.It aims to present the main bioreactor models currently in use for cultivated meat production,detailing their features,advantages,disadvantages,and the technological challenges that need to be addressed.
文摘Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today＇s 2-dimensional（2D） standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef.
