Anthocyanins are plant pigments that play diverse roles in plant growth,adaptation,and stress tolerance.Anthocyanin biosynthesis is tightly regulated,but the underlying regulatory mechanisms remain unclear.Here,we ide...Anthocyanins are plant pigments that play diverse roles in plant growth,adaptation,and stress tolerance.Anthocyanin biosynthesis is tightly regulated,but the underlying regulatory mechanisms remain unclear.Here,we identify a regulatory module composed of the DNA-binding protein VAL1(VIVIPAROUS1/ABI3LIKE 1)and a SIN3(SWI-INDEPENDENT 3)-like histone deacetylase complex that dynamically regulates anthocyanin biosynthesis in Arabidopsis thaliana.Under normal growth conditions,VAL1 recruits the SNL(SIN3-Like)-HDA19(HISTONE DEACETYLASE 19)complex(SNL-HDA19c)to the PRODUCTION OF ANTHOCYANIN PIGMENT 1(PAP1)locus for histone deacetylation.Moreover,the negative regulators of jasmonic acid(JA)signaling,JASMONATE-ZIM DOMAIN(JAZ)proteins,interact with VAL1 and further stabilize the binding of VAL1 and SNL-HDA19c to PAP1 chromatin.These molecular interactions transcriptionally repress PAP1 and inhibit anthocyanin biosynthesis.Upon JA accumulation,JAZs are degraded,resulting in the release of both VAL1 and SNL-HDA19c from the PAP1 chromatin.This release leads to an immediate increase in histone acetylation,promoting transcriptional activation of PAP1 and anthocyanin production.These findings elucidate a regulatory module(VAL1-JAZ-SNL-HDA19c)that represses anthocyanin biosynthesis under normal growth conditions and further reveal how the stress hormone JA rapidly induces anthocyanin production,enabling plants to adapt to their growth conditions.展开更多
Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,b HLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of these MBW subunits remai...Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,b HLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of these MBW subunits remain largely elusive. In this study, we isolated an Arabidopsis mutant that displays a constitutive red color in aboveground tissues with retarded growth phenotypes. In the presence of sucrose, the mutant accumulates more than 3-fold anthocyanins of the wild type(WT), but cannot produce anthocyanins as WT in the absence of sucrose. Map-based cloning results demonstrated that the mutation occurs in the locus At4 G01000, which encodes a conserved nuclear-localized ubiquitin-like(UBL) superfamily protein, silencing defective 2(SDE2), in eukaryotes. SDE2 is ubiquitously expressed in various tissues. In the sucrose-induced anthocyanin biosynthesis, SDE2 expression was not responded to sucrose treatment at the early stage but was enhanced at the late stage. SDE2 mutations result in upregulation of anthocyanin biosynthetic and regulatory genes. Yeast-two hybrid analysis indicated that SDE2 has no direct interaction with the MYB transcription factor PAP1 and b HLH factor TT8, indicating that SDE2 is a indirect factor to affect anthocyanin accumulation. Taking together, our data suggest that SDE2 may play a role in finely coordinating anthocyanin biosynthesis with other biological processes.展开更多
基金supported in part by the National Key Research and Development Program of China(grant no.2024YFF1000301)National Natural Science Foundation of China(grant no.32330007)Peking-Tsinghua Center for Life Sciences,and the Chinese Academy of Sciences.
文摘Anthocyanins are plant pigments that play diverse roles in plant growth,adaptation,and stress tolerance.Anthocyanin biosynthesis is tightly regulated,but the underlying regulatory mechanisms remain unclear.Here,we identify a regulatory module composed of the DNA-binding protein VAL1(VIVIPAROUS1/ABI3LIKE 1)and a SIN3(SWI-INDEPENDENT 3)-like histone deacetylase complex that dynamically regulates anthocyanin biosynthesis in Arabidopsis thaliana.Under normal growth conditions,VAL1 recruits the SNL(SIN3-Like)-HDA19(HISTONE DEACETYLASE 19)complex(SNL-HDA19c)to the PRODUCTION OF ANTHOCYANIN PIGMENT 1(PAP1)locus for histone deacetylation.Moreover,the negative regulators of jasmonic acid(JA)signaling,JASMONATE-ZIM DOMAIN(JAZ)proteins,interact with VAL1 and further stabilize the binding of VAL1 and SNL-HDA19c to PAP1 chromatin.These molecular interactions transcriptionally repress PAP1 and inhibit anthocyanin biosynthesis.Upon JA accumulation,JAZs are degraded,resulting in the release of both VAL1 and SNL-HDA19c from the PAP1 chromatin.This release leads to an immediate increase in histone acetylation,promoting transcriptional activation of PAP1 and anthocyanin production.These findings elucidate a regulatory module(VAL1-JAZ-SNL-HDA19c)that represses anthocyanin biosynthesis under normal growth conditions and further reveal how the stress hormone JA rapidly induces anthocyanin production,enabling plants to adapt to their growth conditions.
基金supported by the National Basic Research Program of China (2013CB127000)the National Natural Science Foundation of China (31370326)
文摘Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,b HLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of these MBW subunits remain largely elusive. In this study, we isolated an Arabidopsis mutant that displays a constitutive red color in aboveground tissues with retarded growth phenotypes. In the presence of sucrose, the mutant accumulates more than 3-fold anthocyanins of the wild type(WT), but cannot produce anthocyanins as WT in the absence of sucrose. Map-based cloning results demonstrated that the mutation occurs in the locus At4 G01000, which encodes a conserved nuclear-localized ubiquitin-like(UBL) superfamily protein, silencing defective 2(SDE2), in eukaryotes. SDE2 is ubiquitously expressed in various tissues. In the sucrose-induced anthocyanin biosynthesis, SDE2 expression was not responded to sucrose treatment at the early stage but was enhanced at the late stage. SDE2 mutations result in upregulation of anthocyanin biosynthetic and regulatory genes. Yeast-two hybrid analysis indicated that SDE2 has no direct interaction with the MYB transcription factor PAP1 and b HLH factor TT8, indicating that SDE2 is a indirect factor to affect anthocyanin accumulation. Taking together, our data suggest that SDE2 may play a role in finely coordinating anthocyanin biosynthesis with other biological processes.
