Over the last decades and centuries,European mountain landscapes have experienced substantial transformations.Natural and anthropogenic LULC changes(land use and land cover changes), especially agro-pastoral activitie...Over the last decades and centuries,European mountain landscapes have experienced substantial transformations.Natural and anthropogenic LULC changes(land use and land cover changes), especially agro-pastoral activities,have directly influenced the spatial organization and composition of European mountain landscapes.For the past sixty years, natural reforestation has been occurring due to a decline in both agricultural production activities and rural population.Stakeholders, to better anticipate future changes,need spatially and temporally explicit models to identify areas at risk of land change and possible abandonment.This paper presents an integrated approach combining forecasting scenarios and a LULC changes simulation model to assess where LULC changes may occur in the Pyrenees Mountains,based on historical LULC trends and a range of future socio-economic drivers.The proposed methodologyconsiders local specificities of the Pyrenean valleys,sub-regional climate and topographical properties,and regional economic policies.Results indicate that some regions are projected to face strong abandonment, regardless of the scenario conditions.Overall, high rates of change are associated with administrative regions where land productivity is highly dependent on socio-economic drivers and climatic and environmental conditions limit intensive(agricultural and/or pastoral) production and profitability.The combination of the results for the four scenarios allows assessments of where encroachment(e.g.colonization by shrublands) and reforestation are the most probable.This assessment intends to provide insight into the potential future development of the Pyrenees to help identify areas that are the most sensitive to change and to guide decision makers to help their management decisions.展开更多
Seasonally frozen ground,mountain permafrost and cryogenic geomorphological processes are important components of the Pyrenean high mountains.This work presents the results of a study on the distribution of frozen gro...Seasonally frozen ground,mountain permafrost and cryogenic geomorphological processes are important components of the Pyrenean high mountains.This work presents the results of a study on the distribution of frozen ground in a marginal and paraglacial environment of temperate mountains.An inventory was made of landforms and indicators of frozen ground,and frozen ground was mapped accordingly.During 2014 and 2016 ground temperatures and thermal regimes were monitored,basal temperatures of snow-cover(BTS)were measured and a thermal map was drawn.Differential thermal behaviours were detected among different elevations and slope orientations.Periglacial processes are the most widespread,in which frost weathering and nivation,together with gelifluction and cryoturbation,are the most efficient processes;the latter two are generally linked to the presence of frozen ground.The fall in air and ground temperatures with altitude,slope orientations,and snowpack thickness and evolution determine ground thermal regimes.In the study area,three types of thermal regimes were established:climate-controlled,snowcover-controlled,and frozen ground-controlled.Seasonally frozen ground occurs across a broad range of elevation between 2650 and 3075 m asl,whereas possible permafrost only occurs above 2750 m asl.展开更多
Livestock behaviour in the Pyrenees includes free grazing and a long resting period that provokes the accumulation of dung and urine in some places,so-called camping areas. The aims of this study were (i) to analyze a...Livestock behaviour in the Pyrenees includes free grazing and a long resting period that provokes the accumulation of dung and urine in some places,so-called camping areas. The aims of this study were (i) to analyze any change in floral composition,and in nutritional and chemical contents of plants in a livestock camping area; and (ii) to relate the floral composition with soil chemical properties. In a linear transect,five sampling zones were established,from the centre of the camping area to the surrounding Nardus stricta-dominant pasture. The above ground plant biomass and the topsoil were sampled in each zone with 6 replicates per zone. Plant species were classified and weighed to calculate above ground biomass,nutritional and chemical contents,and Shannon diversity and evenness indices. Additionally,soils were sampled in two periods,at the beginning and at the end of grazing period. Soil available nutrients (nitrate,ammonium,phosphorus,potassium,calcium and magnesium),total nitrogen,organic carbon and pH were measured. Plant chemical contents (protein,lignin and others) were significantly related to the proportions of grasses,legumes and other plants; so,the protein content is positively correlated with legumes plant biomass while lignin content is negatively correlated with grasses. Both plant and soil nutrients increased linearly towards the centre of the camping area. However,the relationship among plant species richness,diversity and evenness relative to its position along the studied transect was bell-shaped. From the outskirts to the centre of the camping area,plants with low nutrient demand were progressively replaced by those with medium and high nutrients demand and by pioneers. Nardus stricta-dominant pasture has low plant diversity and plant nutrient content as well as a poor soil nutrient availability. The presence of the camping area introduced patches with more soil nutrients and new species in the large spatial scale. However at a small spatial scale,the strong soil nutrient concentration into the centre of the camping area reduced floral composition again. To ensure positive effects of camping areas on plant diversity,and to manage more effectively the nutrients returned to the soil,a reduction in the stocking rate should be pursued.展开更多
Glacial cirques are typical landscape features of mid-latitude mountain environments like the Central Pyrenees. Their morphology as well as their spatial distribution provides insights about past glaciers and climates...Glacial cirques are typical landscape features of mid-latitude mountain environments like the Central Pyrenees. Their morphology as well as their spatial distribution provides insights about past glaciers and climates. In this study, we examine the distribution, morphometrical and topographical characteristics of glacial cirques in two U-shaped glacial valleys located in the Central Pyrenees – the Aran and the Boí valleys. They are located in different aspects of this mountain range(north vs south) under different climatic influences that promoted distinct glaciation patterns during the late Pleistocene. The spatial mapping of these landforms was carried out using high-resolution imagery and field observations. We analysed the data of the morphometrical and topographical variables of the glacial cirques by using different statistical and geospatial methods in order tounveil the factors controlling their formation and development. A total of 186 glacial cirques were mapped in the study area, including 119 in the Aran and 67 in the Boí valleys. The local topography and microclimate conditions lead to substantial differences in both areas in terms of the morphology and dimensions of the cirques. Glacial cirques in Boí are distributed at slightly higher elevations than in Aran and they are also larger, though their dimensions decrease with elevation in both valleys. Aran cirques are mostly oriented NE, while Boí landforms do not show any prevailing aspect. Even though lithology does not control the distribution of the glacial cirques, some specific lithological settings may favour the development of larger cirques. In general, glacial cirques in the Aran and the Boí valleys show morphometrical properties similar to those reported in other mid-latitude mountain ranges.展开更多
The function and conservation of many forest ecosystems depend on the distribution and diversity of the community of rodents that consume and disperse seeds.The habitat preferences and interactions are especially rele...The function and conservation of many forest ecosystems depend on the distribution and diversity of the community of rodents that consume and disperse seeds.The habitat preferences and interactions are especially relevant in alpine systems where such granivorous rodents reach the southernmost limit of their distribution and are especially sensitive to global warming.We analyzed the community of granivorous rodents in the Pyrenees,one of the southernmost mountain ranges of Europe.Rodent species were identified by DNA with particular attention to the Apodemus species,which are prominent seed-dispersing rodents in Europe.We confirmed for the first time the presence of the yellow-necked mouse,Apodemus flavicollis,in central Pyrenees,a typical Eurosiberian species that reaches its southernmost distribution limit in this area.We also found the wood mouse,Apodemus sylvaticus,a related species more tolerant to Mediterranean environments.Both rodents were spatially segregated by altitude.A.sylvaticus was rare at high altitudes,which might cause the genetic differentiation between populations of the different valleys reported here.We also found other seed consumers like dormice,Elyomis quercinus,and voles,Myodes glareolus,with marked habitat preferences.We suggest that population isolation among valleys may increase the genetic diversity of rodents,like A.sylvaticus.We also highlight the potential threat that global warming may represent for species linked to high-altitude refuges at the southern edge of its distribution,like Apodemus flavicollis.Finally,we discuss how this threat may have a dimension in the conservation of alpine forests dispersed by these rodent populations.展开更多
The purpose of this study was to characterize mountain river regimes in the Spanish Pyrenees and to assess the importance of snow accumulation and snowmelt on the timing of river flows. Daily streamflow data from 9 ga...The purpose of this study was to characterize mountain river regimes in the Spanish Pyrenees and to assess the importance of snow accumulation and snowmelt on the timing of river flows. Daily streamflow data from 9 gauging stations in the Pyrenees were used to characterize river regimes. These data were analyzed by hydrological indices, with a focus on periods when snow accumulation and snowmelt occurred. These results were combined with data on Snow Water Equivalent （SWE） （from measure- ments of depth and density of snow in the main river basins and also simulated by a process-based hydrological model）, snowmelting （simulated by a process-based hydrological model）, precipitation （from observations）, and temperature （from observations）. Longitude. and elevation gradients in the Pyrenees explain the transition of river regimes from those that mostly had low nival signals （in the west and at low elevations） to those that mostly had high nival signals （low winter runoff and late spring peakflow, in the east and at high elevations）. Although trend analyses indicated no statistically signifi- cant changes, there was a trend of decreased nival signal over time in most of the analyzed rivers. Our results also demonstrated that snow processes cannot explain all of the interannual variability of river regimes, because the temporal distribution of liquid precipitation and tempera- ture play key roles in hydrography.展开更多
Benzo[a]pyrene(B[a]P)is a carcinogenic environmental pollutant widely present in the environment and can enter the human body through the food chain.It is therefore essential to treat and remediate the B[a]P-contamina...Benzo[a]pyrene(B[a]P)is a carcinogenic environmental pollutant widely present in the environment and can enter the human body through the food chain.It is therefore essential to treat and remediate the B[a]P-contaminated environment.Microbial remediation of B[a]Pcontaminated environments is considered to be one of the most effective strategies,and the addition of biostimulants is a feasible method to further improve the effectiveness of microbial remediation.In this study,we used Bacillus subtilis MSC4 to screen for the stimulation of sodium gluconate,which promoted B[a]P degradation.Based on biochemical and transcriptomic analyses,Sodium gluconate was found to significantly increase the biomass of MSC4 and the expression of most genes involved in B[a]P degradation.Activities of central carbon metabolism,fatty acidβ-oxidation and oxidative phosphorylation were all promoted.The significant increase in acid-induced oxalate decarboxylase expression indicates a decrease in intracellular pH,which promoted the synthesis of acetoin and lactate.Genes involved in the nitrogen cycle,especially nitrification and denitrification,were significantly up-regulated,contributing to B[a]P degradation.Genes involved in the synthesis of enzyme cofactors,including thiamine,molybdenum cofactors,NAD and heme,were up-regulated,which contributes to increasing enzyme activity in metabolic pathways.Up-regulation of genes in flagella assembly,chemotaxis,and lipopeptide synthesis is beneficial for the dissolution and uptake of B[a]P.Genes related to the sugar transport system were upregulated,which facilitates the transport and absorption of monosaccharides and oligosaccharides by MSC4.This study provides a theoretical basis for the further application of sodium gluconate in the treatment of PAH-contaminated sites.展开更多
Six coordination polymers based on 9,10-di(pyridine-4-yl)-anthracene(DPA)and 1,6-di(1H-imidazol-1-yl)pyrene(DIP)were obtained by solvothermal reactions.{[Zn(DPA)Cl_(2)]·DMF·2H_(2)O}n(1)and{[Zn_(1.5)(DPA)_(1....Six coordination polymers based on 9,10-di(pyridine-4-yl)-anthracene(DPA)and 1,6-di(1H-imidazol-1-yl)pyrene(DIP)were obtained by solvothermal reactions.{[Zn(DPA)Cl_(2)]·DMF·2H_(2)O}n(1)and{[Zn_(1.5)(DPA)_(1.5)Cl_(3)]·5H_(2)O}n(2)are framework isomers,which both contain zigzag chains formed by DPA,Zn^(2+),and Cl-.The zigzag chains in 1 are further assembled by C—H…Cl interactions into layers,and these layers exhibit two different orientations,displaying a rare 2D to 3D interpenetration mode.The zigzag chains in 2 are parallelly arranged.{[Zn_(3)(DPA)_(3)Br_(6)]·2DMF·_(1.5)H_(2)O}n(3)is isostructural to 2.3 was obtained using ZnBr_(2)instead of ZnCl_(2).[M(DPA)(formate)_(2)(H_(2)O)_(2)]n[M=Co(4),Cu(5)]are isostructural,contain chain structures formed by DPA,Cu^(2+)/Co^(2+),and for-mate ions,which were formed in situ in the solvothermal reaction.{[Zn(DIP)_(2)Cl]ClO_(4)}n(6)contains a layer structure formed by DIP and Zn^(2+).Free DPA and DIP ligands exhibited high fluorescence at room temperature,and coordina-tion polymers 3 and 6 displayed enhanced fluorescent emissions.展开更多
We report five coordination polymers(CPs)based on fluorescent ligands[1,6-di(1H-imidazol-1-yl)pyrene(dip),9,10-di(1H-imidazol-1-yl)anthracene(dia)]and anionic ligands[cyclohexane-1,4-dicarboxylic acid(H_(2)cda),campho...We report five coordination polymers(CPs)based on fluorescent ligands[1,6-di(1H-imidazol-1-yl)pyrene(dip),9,10-di(1H-imidazol-1-yl)anthracene(dia)]and anionic ligands[cyclohexane-1,4-dicarboxylic acid(H_(2)cda),camphoric acid(H_(2)cpa)].In[Cd(dip)(cda)]·4H_(2)O}_(n)(1),the Cd^(2+)ions,acting as tetrahedral nodes,are linked by dipand cda^(2-)ligands with four Cd^(2+)ions into five-fold interpenetrating network array of topology of dia.In{[Cd(dip)(cpa)]·4H_(2)O}_(n)(2),the Cd^(2+)ions,acting as a 4-connector,are linked by cpa^(2-)and dip ligands into a 3D framework ofcds topology.In{[Ni(dia)_(2)Cl_(2)]·DMF}_(n)(3),the Ni^(2+)ion is linked by four dia ligands into a layer structure,and 1Dchannels of a cross-section of 1.35 nm×0.96 nm are formed.In{[Cd(dia)_(2)(H_(2)O)_(2)](NO_(3))_(2)·2DMSO}n(4),the dia ligandsconnected Cd^(2+)ions into a 2D layer,and 1D channels are formed between adjacent layers with a cross-section of0.87 nm×0.43 nm.In[Zn(dip)Cl_(2)]_(n)(5),the Zn^(2+)ion is linked by dip ligands into an infinite 1D chain.The infrared,thermal gravimetric,and fluorescent emission data were collected and analyzed for these coordination polymers.CCDC:2356055,1;2440075,2;2356057,3;2356057,4;2356059,5.展开更多
Epidemiological studies indicate that prostate cancer(PCa)is the second prevalent malignant tumor affecting men globally.Environmental pollution such as cigarette smoke is one of the important risk factors for the dev...Epidemiological studies indicate that prostate cancer(PCa)is the second prevalent malignant tumor affecting men globally.Environmental pollution such as cigarette smoke is one of the important risk factors for the development of prostate cancer.However,as one of the main carcinogens in cigarette smoke,the role of benzo[a]pyrene(BaP)in prostate cancer is still unclear.The current study aimed to investgate the impacts of BaP exposure on the progression of PCa towardmalignancy and the regulation of the immune microenvironment.We verified that BaP exposure can promote the proliferation,migration,and apoptosis of prostate cancer cells through in vitro experiments.We constructed a subcutaneous xenograft tumor model of BaP exposure mouse and found that can promote the proliferation of tumors in vivo.Organoids-driven by PCa patients showed higher growth rate under BaP exposure.Flow cytometric analysis demonstrated a remarkable decrease in CD4^(+)T and CD8^(+)T cell infiltration levels.Moreover,we identified four genes(Mdm2,Ar,Foxo1,Crebbp)were strongly associated with BaP exposure by combining mouse tumor RNA-seq and CTD database.Additionally,a nomogram integrating clinicopathological features was constructed to assess the prognosis of prostate cancer patients under BaP exposure.This study systematically proved that BaP exposure promotes malignant progression of PCa and suppresses the immune microenvironment,in which Mdm2,Ar,Foxo1,Crebbp may play a crucial role in inhibiting apoptosis.These findings offer novel insights into themechanisms via which BaP exposure contributes to PCa development.展开更多
Peanut is a globally important leguminous crop and one of the most important oil crops.In response to the growing demand for high-quality peanut oil,advancements in processing technologies have led to significant impr...Peanut is a globally important leguminous crop and one of the most important oil crops.In response to the growing demand for high-quality peanut oil,advancements in processing technologies have led to significant improvements in oil quality.However,ensuring consistent quality remains a complex and ongoing challenge due to the multifaceted factors influencing peanut oil’s properties.This review synthesizes key scientific studies addressing these factors and explores the associated risks to oil quality and safety.Special attention is given to harmful contaminants such as aflatoxin B1(AFB1),3-chloro-1,2-propanediol esters(3-MCPDE),Benzo[a]pyrene(BaP),and trans-fatty acids(TFAs),which pose significant health risks and quality concerns.The review critically examines current detection methods for these contaminants and evaluates innovative removal strategies,such as biodegradation,physical refining,chemical treatments,and advanced adsorption techniques.Moreover,insights into the effects of raw material quality,processing conditions,and storage on oil quality were discussed.In conclusion,the review underscores the importance of adopting integrated approaches to control harmful substances while optimizing processing parameters to enhance peanut oil quality.These findings aim to guide researchers and industry practitioners in improving production practices,minimizing health risks,and providing safer and higher-quality peanut oil products for consumers.展开更多
Marine pollution poses a critical threat to biodiversity.The synergistic effects of microplastics,specifically high-density polyethylene(HDPE),combined with benzo[a]pyrene(B[a]P)and heavy metals,on the physiology and ...Marine pollution poses a critical threat to biodiversity.The synergistic effects of microplastics,specifically high-density polyethylene(HDPE),combined with benzo[a]pyrene(B[a]P)and heavy metals,on the physiology and feeding behavior of the seahorse,Hippocampus erectus were studied.The growth parameters,pollutant bioaccumulation,enzymatic response,and feeding dynamics were extensively analyzed.Seahorses exposed to microplastics-borne B[a]P and heavy metals exhibited notable reductions in body length and weight,as well as in survival rates,indicating severe stress from these contaminants.The accumulation of pollutant particularly B[a]P in seahorse exceeded China’s national regulatory limit of 5μg/kg,and the B[a]P accumulation in seahorses was obviously aided by HDPE microplastics,posing a risk to marine organisms and human health via the food chain.The adaptive response of the seahorses was evident shown in the elevation of antioxidant enzyme activities,which is a biological mechanism to mitigate oxidative stress induced by the pollutants.However,this physiological adaptation comes at a cost,as evidenced by marked changes in the feeding behavior.Results show that the efficiency and frequency of feeding were greatly reduced,indicating a heavy impact on the ecological role and survival of this marine creature in polluted habitats.This research offers a crucial perspective on the compound threats of microplastics and associated pollutants in marine ecosystems.It highlights the urgent need develop comprehensive strategies to address these environmental issues.The findings are an important contribution to ecotoxicology,providing a deeper understanding of how microplastics act as vectors for other contaminants and their profound effects on marine life,especially species like seahorses.This study calls for action to strengthen environmental policies and practices aimed at mitigating the impact of marine pollution.展开更多
High molecular weight polycyclic aromatic hydrocarbons(HMW-PAHs)pose significant environmental challenges due to their complex structures and persistent toxicity,and are difficult to be degraded by bacteria.Fungi,howe...High molecular weight polycyclic aromatic hydrocarbons(HMW-PAHs)pose significant environmental challenges due to their complex structures and persistent toxicity,and are difficult to be degraded by bacteria.Fungi,however,possess the ability to overcome these challenges,primarily through various enzymes with broad substrate specificity,including cytochrome P450(CYP450),laccase,manganese peroxidase,lignin peroxidase.As a result,the development of efficient fungal strains capable of degrading HMW-PAHs is essential for advancing bioremediation strategies.In this study,Aspergillus fumigatus Z5 was isolated from a contaminated site and demonstrated a remarkable ability to degrade the highly recalcitrant benzo[a]pyrene,achieving a degradation rate of 75.43%.Transcriptomic analysis revealed significant upregulation of 34 CYP450 genes.Among these genes,CYP3A4(gene 8840)showed strong binding affinity for benzo[a]pyrene,as confirmed by molecular docking studies,indicating its key role in the biodegradable process.Furthermore,the metabolic network analysis showed that the electron transfer required for CYP450-mediated oxidative reactions enhanced mitochondrial oxidative phosphorylation and subsequently energy metabolism in A.fumigatus Z5.This metabolic coordination likely facilitates the complete degradation of benzo[a]pyrene.Our study illuminates the fundamental roles of CYP450 from A.fumigatus Z5 in benzo[a]pyrene degradation and provides novel insight into designing and implementing enhanced bioremediation strategies.展开更多
[Objective]The paper was to explore the effects of pyrene stress on growth and physiological indexes of maize seedling.[Method] The effects of different concentrations of pyrene（0,0.5,1.0,and 2.0 mg/L） on growth and...[Objective]The paper was to explore the effects of pyrene stress on growth and physiological indexes of maize seedling.[Method] The effects of different concentrations of pyrene（0,0.5,1.0,and 2.0 mg/L） on growth and several physiological indexes,such as chlorophyll content,malnodiadehyde（MDA） content of both leaves and roots of maize（Zea Mays L.） seedlings of Shixiang 1（sensitive variety） and Guangtian 3（tolerant variety） were studied by a hydroponic experiment.[Result]As the concentration of prene increasing within the test concentration range,the contents of chlorophyll a and chlorophyll b in Shixiang 1 first increased and then decreased;relative growth yield and absolute growth rate showed the decreasing trend;MDA contents in roots and leaves showed the increasing trend.Relative growth yield,absolute growth rate and chlorophyll a content in Guangtian 3 first increased and then decreased;chlorophyll b and MDA contents in roots showed the increasing trend;MDA contents in leaves showed the decreasing trend.Compared with control,chlorophyll a and chlorophyll b contents of Shixiang 1 significantly increased under pyrene concentration of 0.5-1.0 mg/L,which significantly decreased as the concentration of pyrene ≥2.0 mg/L;MDA contents in roots and leaves significantly increased as the concentration of pyrene ≥1.0 mg/L,while relative growth yield and absolute growth rate significantly decreased.Chlorophyll a and chlorophyll b contents in Guangtian 3 significantly increased as the concentration of pyrene ≥1.0 mg/L.Pyrene concentration had significantly negative correlation with relative growth yield and absolute growth rate of Shixiang 1,while it had significant positive correlation with MDA contents in roots.Pyrene concentration had significant positive correlation with chlorophyll b and MDA contents in roots of tolerant variety Guangtian 3.[Conclusion]The study provided basis for the management measures of maize during its seedling stage.展开更多
This study demonstrated the usefulness of very long-range terrestrial laser scanning(TLS) for analysis of the spatial distribution of a snowpack, to distances up to 3000 m, one of the longest measurement range reporte...This study demonstrated the usefulness of very long-range terrestrial laser scanning(TLS) for analysis of the spatial distribution of a snowpack, to distances up to 3000 m, one of the longest measurement range reported to date. Snow depth data were collected using a terrestrial laser scanner during 11 periods of snow accumulation and melting,over three snow seasons on a Pyrenean hillslopecharacterized by a large elevational gradient, steep slopes, and avalanche occurrence. The maximum and mean absolute snow depth error found was 0.5-0.6 and 0.2-0.3 m respectively, which may result problematic for areas with a shallow snowpack, but it is sufficiently accurate to determine snow distribution patterns in areas characterized by a thick snowpack. The results indicated that in most cases there was temporal consistency in the spatial distribution of thesnowpack, even in different years. The spatial patterns were particularly similar amongst thesurveys conducted during the period dominated by snow accumulation(generally until end of April), or amongst those conducted during the period dominated by melting processes(generally after mid of April or early May). Simple linear correlation analyses for the 11 survey dates, and the application of Random Forests analysis to two days representative of snow accumulation and melting periods indicated the importance of topography to the snow distribution. The results also highlight that elevation and the Topographic Position index(TPI) were the main variables explaining the snow distribution, especially during periods dominated by melting. The intra-and inter-annual spatial consistency of the snowpack distribution suggests that the geomorphological processes linked to presence/absence of snow cover act in a similar way in the long term, and that these spatial patternscan be easily identifiedthrough several years of adequate monitoring.展开更多
AIM To study the inhibitory effects of VES( RRR-α-tocopheryl Succinate, VES ), aderivative of natural Vitamin E, on benzo (a)pyrene (B (a) P)-induced forestomach tumor infemale mice.METHODS The model of B (a)P-induce...AIM To study the inhibitory effects of VES( RRR-α-tocopheryl Succinate, VES ), aderivative of natural Vitamin E, on benzo (a)pyrene (B (a) P)-induced forestomach tumor infemale mice.METHODS The model of B (a)P-inducedforestomach tumor was established according tothe methods of Wattenberg with slightmodifications. One hundred and eighty femalemice (6 weeks old) were divided into six groupsequally; negative control (Succinic acid),vehicle control ( Succinate + B (a) P), positivecontrol(B(a) P), high VES(2.5g/kg. b. w + B(a)P), Iow VES(1 .25 g/kg. b. w + B(a) P) ig as wellas VES by ip (20 mg/kg, b. w + B(a) P). Exceptthe negative control group, the mice wereadministrated with B(a)P ig. and correspondingtreatments for 4 weeks to study the anti-carcinogenetic effect of VES during the initiationperiod. The experiment lasted 29 weeks, inwhich the inhibitory effects of VES both ontumor incidence and tumor size were tested.RESULTS The models of B (a)P-inducedforestomach tumor in female mice wereestablished successfully. Some werecauliflower-like, others looked like papilla, evena few were formed into the ulcer cavities. VES at1.25 g/kg. b. w, 2.5 g/kg. b.w. by ig and 20 mg/kg. b. w. via ip could decrease the number oftumors per mouse (1.7 ± 0. 41, 1.6 ± 0.34 and 1.1±0.43), being lower than that of B(a)P group(5.4 ± 0.32, P＜0.05). The tumor incidence wasinhibited by 18.2%, 23.1% and 50.0%. VES at1.25g/kg.b.w., 2.5 g/ kg.b.w. by ig and20 mg/kg. b.w. via ip reduced the total volumeof tumors per mouse (54.8 ± 8.84, 28.4 ± 8.32and 23.9± 16.05), being significantly lower thanthat of B(a)P group (150.2±20.93, P＜0.01).The inhibitory rates were 63.5%, 81.1% and84.1%, respectively.CONCLUSION VES has inhibitory effects on B(a) P-induced forestomach carcinogenesis infemale mice, especially by ip and it may be apotential anti-cancer agent in vivo.展开更多
Two indigenous microorganisms, Bacillus sp. SB02 and Mucor sp. SF06, capable of degrading polycyclic aromatic hydrocarbons （PAHs） were co-immobilized on vermiculite by physical adsorption and used to degrade benzo[a...Two indigenous microorganisms, Bacillus sp. SB02 and Mucor sp. SF06, capable of degrading polycyclic aromatic hydrocarbons （PAHs） were co-immobilized on vermiculite by physical adsorption and used to degrade benzo[a] pyrene （BaP）. The characteristics of BaP degradation by both free and co-immobilized microorganism were then investigated and compared. The removal rate using the immobilized bacterial-fungal mixed consortium was higher than that of the freely mobile mixed consortium. 95.3% of BaP was degraded using the co-immobilized system within 42 d, which was remarkably higher than the removal rate of that by the free strains. The optimal amount of inoculated co-immobilized system for BaP degradation was 2%. The immobilized bacterial-fungal mixed consortium also showed better water stability than the free strains. Kinetics of BaP biodegradation by co-immobilized SF06 and SB02 were also studied. The results demonstrated that BaP degradation could be well described by a zero-order reaction rate equation when the initial BaP concentration was in the range of 10--200 mg/kg. The scanning electronic microscope （SEM） analysis showed that the co-immobilized microstructure was suitable for the growth of SF06 and SB02. The mass transmission process of co-immobilized system in soil is discussed. The results demonstrate the potential for employing the bacterial-fungal mixed consortium, co-immobilized on vermiculite, for in situ bioremediation of BaP.展开更多
基金supported by the MODE RESPYR project(ANR 2010 JCJC 1804-01)founded by the French National Science Agency(ANR)
文摘Over the last decades and centuries,European mountain landscapes have experienced substantial transformations.Natural and anthropogenic LULC changes(land use and land cover changes), especially agro-pastoral activities,have directly influenced the spatial organization and composition of European mountain landscapes.For the past sixty years, natural reforestation has been occurring due to a decline in both agricultural production activities and rural population.Stakeholders, to better anticipate future changes,need spatially and temporally explicit models to identify areas at risk of land change and possible abandonment.This paper presents an integrated approach combining forecasting scenarios and a LULC changes simulation model to assess where LULC changes may occur in the Pyrenees Mountains,based on historical LULC trends and a range of future socio-economic drivers.The proposed methodologyconsiders local specificities of the Pyrenean valleys,sub-regional climate and topographical properties,and regional economic policies.Results indicate that some regions are projected to face strong abandonment, regardless of the scenario conditions.Overall, high rates of change are associated with administrative regions where land productivity is highly dependent on socio-economic drivers and climatic and environmental conditions limit intensive(agricultural and/or pastoral) production and profitability.The combination of the results for the four scenarios allows assessments of where encroachment(e.g.colonization by shrublands) and reforestation are the most probable.This assessment intends to provide insight into the potential future development of the Pyrenees to help identify areas that are the most sensitive to change and to guide decision makers to help their management decisions.
基金funded by I+D+I projects CGL2015-68144-R and CGL2017-82216-R(MINECO of Spanish government-FEDER)Geoparque Sobrarbe-Comarca del Sobrabe(R-ADM15/57)
文摘Seasonally frozen ground,mountain permafrost and cryogenic geomorphological processes are important components of the Pyrenean high mountains.This work presents the results of a study on the distribution of frozen ground in a marginal and paraglacial environment of temperate mountains.An inventory was made of landforms and indicators of frozen ground,and frozen ground was mapped accordingly.During 2014 and 2016 ground temperatures and thermal regimes were monitored,basal temperatures of snow-cover(BTS)were measured and a thermal map was drawn.Differential thermal behaviours were detected among different elevations and slope orientations.Periglacial processes are the most widespread,in which frost weathering and nivation,together with gelifluction and cryoturbation,are the most efficient processes;the latter two are generally linked to the presence of frozen ground.The fall in air and ground temperatures with altitude,slope orientations,and snowpack thickness and evolution determine ground thermal regimes.In the study area,three types of thermal regimes were established:climate-controlled,snowcover-controlled,and frozen ground-controlled.Seasonally frozen ground occurs across a broad range of elevation between 2650 and 3075 m asl,whereas possible permafrost only occurs above 2750 m asl.
文摘Livestock behaviour in the Pyrenees includes free grazing and a long resting period that provokes the accumulation of dung and urine in some places,so-called camping areas. The aims of this study were (i) to analyze any change in floral composition,and in nutritional and chemical contents of plants in a livestock camping area; and (ii) to relate the floral composition with soil chemical properties. In a linear transect,five sampling zones were established,from the centre of the camping area to the surrounding Nardus stricta-dominant pasture. The above ground plant biomass and the topsoil were sampled in each zone with 6 replicates per zone. Plant species were classified and weighed to calculate above ground biomass,nutritional and chemical contents,and Shannon diversity and evenness indices. Additionally,soils were sampled in two periods,at the beginning and at the end of grazing period. Soil available nutrients (nitrate,ammonium,phosphorus,potassium,calcium and magnesium),total nitrogen,organic carbon and pH were measured. Plant chemical contents (protein,lignin and others) were significantly related to the proportions of grasses,legumes and other plants; so,the protein content is positively correlated with legumes plant biomass while lignin content is negatively correlated with grasses. Both plant and soil nutrients increased linearly towards the centre of the camping area. However,the relationship among plant species richness,diversity and evenness relative to its position along the studied transect was bell-shaped. From the outskirts to the centre of the camping area,plants with low nutrient demand were progressively replaced by those with medium and high nutrients demand and by pioneers. Nardus stricta-dominant pasture has low plant diversity and plant nutrient content as well as a poor soil nutrient availability. The presence of the camping area introduced patches with more soil nutrients and new species in the large spatial scale. However at a small spatial scale,the strong soil nutrient concentration into the centre of the camping area reduced floral composition again. To ensure positive effects of camping areas on plant diversity,and to manage more effectively the nutrients returned to the soil,a reduction in the stocking rate should be pursued.
基金supported by the Research Group Climate Change and Environmental Systems(ZEPHYRUS)of the Institute of GeographySpatial Planning of the University of Lisbon+4 种基金a grant from the Erasmus+LLP Programme Grant funding the research stay of Luis Lopes at the University of Barcelonasupported by the Ramón y Cajal Program of the Spanish Ministry of Economy and Competitiveness(RYC-2015-17597)Financial support was also provided by the research group ANTALP(Antarctic,Arctic and Alpine environments,2017-SGR-1102)the PALEOGREEN(CTM2017-87976-P)CRONOANTAR(CTM2016-77878-P)projects of the Spanish Ministry of Economy and Competitiveness
文摘Glacial cirques are typical landscape features of mid-latitude mountain environments like the Central Pyrenees. Their morphology as well as their spatial distribution provides insights about past glaciers and climates. In this study, we examine the distribution, morphometrical and topographical characteristics of glacial cirques in two U-shaped glacial valleys located in the Central Pyrenees – the Aran and the Boí valleys. They are located in different aspects of this mountain range(north vs south) under different climatic influences that promoted distinct glaciation patterns during the late Pleistocene. The spatial mapping of these landforms was carried out using high-resolution imagery and field observations. We analysed the data of the morphometrical and topographical variables of the glacial cirques by using different statistical and geospatial methods in order tounveil the factors controlling their formation and development. A total of 186 glacial cirques were mapped in the study area, including 119 in the Aran and 67 in the Boí valleys. The local topography and microclimate conditions lead to substantial differences in both areas in terms of the morphology and dimensions of the cirques. Glacial cirques in Boí are distributed at slightly higher elevations than in Aran and they are also larger, though their dimensions decrease with elevation in both valleys. Aran cirques are mostly oriented NE, while Boí landforms do not show any prevailing aspect. Even though lithology does not control the distribution of the glacial cirques, some specific lithological settings may favour the development of larger cirques. In general, glacial cirques in the Aran and the Boí valleys show morphometrical properties similar to those reported in other mid-latitude mountain ranges.
基金This work was supported by the projects IMPACTBOAR(394/2011)and PLAGANADO AGL2014-54739-9R.B.was funded by a contract of the Atracción de Talento Investigador Programme(Gobierno de Extremadura TA13032).
文摘The function and conservation of many forest ecosystems depend on the distribution and diversity of the community of rodents that consume and disperse seeds.The habitat preferences and interactions are especially relevant in alpine systems where such granivorous rodents reach the southernmost limit of their distribution and are especially sensitive to global warming.We analyzed the community of granivorous rodents in the Pyrenees,one of the southernmost mountain ranges of Europe.Rodent species were identified by DNA with particular attention to the Apodemus species,which are prominent seed-dispersing rodents in Europe.We confirmed for the first time the presence of the yellow-necked mouse,Apodemus flavicollis,in central Pyrenees,a typical Eurosiberian species that reaches its southernmost distribution limit in this area.We also found the wood mouse,Apodemus sylvaticus,a related species more tolerant to Mediterranean environments.Both rodents were spatially segregated by altitude.A.sylvaticus was rare at high altitudes,which might cause the genetic differentiation between populations of the different valleys reported here.We also found other seed consumers like dormice,Elyomis quercinus,and voles,Myodes glareolus,with marked habitat preferences.We suggest that population isolation among valleys may increase the genetic diversity of rodents,like A.sylvaticus.We also highlight the potential threat that global warming may represent for species linked to high-altitude refuges at the southern edge of its distribution,like Apodemus flavicollis.Finally,we discuss how this threat may have a dimension in the conservation of alpine forests dispersed by these rodent populations.
文摘The purpose of this study was to characterize mountain river regimes in the Spanish Pyrenees and to assess the importance of snow accumulation and snowmelt on the timing of river flows. Daily streamflow data from 9 gauging stations in the Pyrenees were used to characterize river regimes. These data were analyzed by hydrological indices, with a focus on periods when snow accumulation and snowmelt occurred. These results were combined with data on Snow Water Equivalent （SWE） （from measure- ments of depth and density of snow in the main river basins and also simulated by a process-based hydrological model）, snowmelting （simulated by a process-based hydrological model）, precipitation （from observations）, and temperature （from observations）. Longitude. and elevation gradients in the Pyrenees explain the transition of river regimes from those that mostly had low nival signals （in the west and at low elevations） to those that mostly had high nival signals （low winter runoff and late spring peakflow, in the east and at high elevations）. Although trend analyses indicated no statistically signifi- cant changes, there was a trend of decreased nival signal over time in most of the analyzed rivers. Our results also demonstrated that snow processes cannot explain all of the interannual variability of river regimes, because the temporal distribution of liquid precipitation and tempera- ture play key roles in hydrography.
基金supported by the National Key R&D Program of China(No.2020YFC1808803).
文摘Benzo[a]pyrene(B[a]P)is a carcinogenic environmental pollutant widely present in the environment and can enter the human body through the food chain.It is therefore essential to treat and remediate the B[a]P-contaminated environment.Microbial remediation of B[a]Pcontaminated environments is considered to be one of the most effective strategies,and the addition of biostimulants is a feasible method to further improve the effectiveness of microbial remediation.In this study,we used Bacillus subtilis MSC4 to screen for the stimulation of sodium gluconate,which promoted B[a]P degradation.Based on biochemical and transcriptomic analyses,Sodium gluconate was found to significantly increase the biomass of MSC4 and the expression of most genes involved in B[a]P degradation.Activities of central carbon metabolism,fatty acidβ-oxidation and oxidative phosphorylation were all promoted.The significant increase in acid-induced oxalate decarboxylase expression indicates a decrease in intracellular pH,which promoted the synthesis of acetoin and lactate.Genes involved in the nitrogen cycle,especially nitrification and denitrification,were significantly up-regulated,contributing to B[a]P degradation.Genes involved in the synthesis of enzyme cofactors,including thiamine,molybdenum cofactors,NAD and heme,were up-regulated,which contributes to increasing enzyme activity in metabolic pathways.Up-regulation of genes in flagella assembly,chemotaxis,and lipopeptide synthesis is beneficial for the dissolution and uptake of B[a]P.Genes related to the sugar transport system were upregulated,which facilitates the transport and absorption of monosaccharides and oligosaccharides by MSC4.This study provides a theoretical basis for the further application of sodium gluconate in the treatment of PAH-contaminated sites.
文摘Six coordination polymers based on 9,10-di(pyridine-4-yl)-anthracene(DPA)and 1,6-di(1H-imidazol-1-yl)pyrene(DIP)were obtained by solvothermal reactions.{[Zn(DPA)Cl_(2)]·DMF·2H_(2)O}n(1)and{[Zn_(1.5)(DPA)_(1.5)Cl_(3)]·5H_(2)O}n(2)are framework isomers,which both contain zigzag chains formed by DPA,Zn^(2+),and Cl-.The zigzag chains in 1 are further assembled by C—H…Cl interactions into layers,and these layers exhibit two different orientations,displaying a rare 2D to 3D interpenetration mode.The zigzag chains in 2 are parallelly arranged.{[Zn_(3)(DPA)_(3)Br_(6)]·2DMF·_(1.5)H_(2)O}n(3)is isostructural to 2.3 was obtained using ZnBr_(2)instead of ZnCl_(2).[M(DPA)(formate)_(2)(H_(2)O)_(2)]n[M=Co(4),Cu(5)]are isostructural,contain chain structures formed by DPA,Cu^(2+)/Co^(2+),and for-mate ions,which were formed in situ in the solvothermal reaction.{[Zn(DIP)_(2)Cl]ClO_(4)}n(6)contains a layer structure formed by DIP and Zn^(2+).Free DPA and DIP ligands exhibited high fluorescence at room temperature,and coordina-tion polymers 3 and 6 displayed enhanced fluorescent emissions.
文摘We report five coordination polymers(CPs)based on fluorescent ligands[1,6-di(1H-imidazol-1-yl)pyrene(dip),9,10-di(1H-imidazol-1-yl)anthracene(dia)]and anionic ligands[cyclohexane-1,4-dicarboxylic acid(H_(2)cda),camphoric acid(H_(2)cpa)].In[Cd(dip)(cda)]·4H_(2)O}_(n)(1),the Cd^(2+)ions,acting as tetrahedral nodes,are linked by dipand cda^(2-)ligands with four Cd^(2+)ions into five-fold interpenetrating network array of topology of dia.In{[Cd(dip)(cpa)]·4H_(2)O}_(n)(2),the Cd^(2+)ions,acting as a 4-connector,are linked by cpa^(2-)and dip ligands into a 3D framework ofcds topology.In{[Ni(dia)_(2)Cl_(2)]·DMF}_(n)(3),the Ni^(2+)ion is linked by four dia ligands into a layer structure,and 1Dchannels of a cross-section of 1.35 nm×0.96 nm are formed.In{[Cd(dia)_(2)(H_(2)O)_(2)](NO_(3))_(2)·2DMSO}n(4),the dia ligandsconnected Cd^(2+)ions into a 2D layer,and 1D channels are formed between adjacent layers with a cross-section of0.87 nm×0.43 nm.In[Zn(dip)Cl_(2)]_(n)(5),the Zn^(2+)ion is linked by dip ligands into an infinite 1D chain.The infrared,thermal gravimetric,and fluorescent emission data were collected and analyzed for these coordination polymers.CCDC:2356055,1;2440075,2;2356057,3;2356057,4;2356059,5.
基金supported by the National Key Research and Development Program of China(No.2022YFB3804504)the National Natural Science Foundation of China,Youth Project(Nos.82101838,82203367,82203505,and 82203403)the Experimental Animal Fund of Shanghai Science and Technology Commission(No.22140903800).
文摘Epidemiological studies indicate that prostate cancer(PCa)is the second prevalent malignant tumor affecting men globally.Environmental pollution such as cigarette smoke is one of the important risk factors for the development of prostate cancer.However,as one of the main carcinogens in cigarette smoke,the role of benzo[a]pyrene(BaP)in prostate cancer is still unclear.The current study aimed to investgate the impacts of BaP exposure on the progression of PCa towardmalignancy and the regulation of the immune microenvironment.We verified that BaP exposure can promote the proliferation,migration,and apoptosis of prostate cancer cells through in vitro experiments.We constructed a subcutaneous xenograft tumor model of BaP exposure mouse and found that can promote the proliferation of tumors in vivo.Organoids-driven by PCa patients showed higher growth rate under BaP exposure.Flow cytometric analysis demonstrated a remarkable decrease in CD4^(+)T and CD8^(+)T cell infiltration levels.Moreover,we identified four genes(Mdm2,Ar,Foxo1,Crebbp)were strongly associated with BaP exposure by combining mouse tumor RNA-seq and CTD database.Additionally,a nomogram integrating clinicopathological features was constructed to assess the prognosis of prostate cancer patients under BaP exposure.This study systematically proved that BaP exposure promotes malignant progression of PCa and suppresses the immune microenvironment,in which Mdm2,Ar,Foxo1,Crebbp may play a crucial role in inhibiting apoptosis.These findings offer novel insights into themechanisms via which BaP exposure contributes to PCa development.
基金support from National Natural Science Foundation of China(22401080)the Start-up Grant of Henan University of Technology(2023BS006)+1 种基金the Scientific and Technological Research Project of Henan Provincial Science and Technology Department(252102310394)The Youth Found of the Natural Science Foundation of Henan Province(242300420463).
文摘Peanut is a globally important leguminous crop and one of the most important oil crops.In response to the growing demand for high-quality peanut oil,advancements in processing technologies have led to significant improvements in oil quality.However,ensuring consistent quality remains a complex and ongoing challenge due to the multifaceted factors influencing peanut oil’s properties.This review synthesizes key scientific studies addressing these factors and explores the associated risks to oil quality and safety.Special attention is given to harmful contaminants such as aflatoxin B1(AFB1),3-chloro-1,2-propanediol esters(3-MCPDE),Benzo[a]pyrene(BaP),and trans-fatty acids(TFAs),which pose significant health risks and quality concerns.The review critically examines current detection methods for these contaminants and evaluates innovative removal strategies,such as biodegradation,physical refining,chemical treatments,and advanced adsorption techniques.Moreover,insights into the effects of raw material quality,processing conditions,and storage on oil quality were discussed.In conclusion,the review underscores the importance of adopting integrated approaches to control harmful substances while optimizing processing parameters to enhance peanut oil quality.These findings aim to guide researchers and industry practitioners in improving production practices,minimizing health risks,and providing safer and higher-quality peanut oil products for consumers.
基金Supported by the Marine Economic Development Project(No.GDNRC[2022]36)the National Key Research and Development Program of China(No.2018YFD0900206)the Tianjin Science and Technology Program Project(No.21YDTPJC00340)。
文摘Marine pollution poses a critical threat to biodiversity.The synergistic effects of microplastics,specifically high-density polyethylene(HDPE),combined with benzo[a]pyrene(B[a]P)and heavy metals,on the physiology and feeding behavior of the seahorse,Hippocampus erectus were studied.The growth parameters,pollutant bioaccumulation,enzymatic response,and feeding dynamics were extensively analyzed.Seahorses exposed to microplastics-borne B[a]P and heavy metals exhibited notable reductions in body length and weight,as well as in survival rates,indicating severe stress from these contaminants.The accumulation of pollutant particularly B[a]P in seahorse exceeded China’s national regulatory limit of 5μg/kg,and the B[a]P accumulation in seahorses was obviously aided by HDPE microplastics,posing a risk to marine organisms and human health via the food chain.The adaptive response of the seahorses was evident shown in the elevation of antioxidant enzyme activities,which is a biological mechanism to mitigate oxidative stress induced by the pollutants.However,this physiological adaptation comes at a cost,as evidenced by marked changes in the feeding behavior.Results show that the efficiency and frequency of feeding were greatly reduced,indicating a heavy impact on the ecological role and survival of this marine creature in polluted habitats.This research offers a crucial perspective on the compound threats of microplastics and associated pollutants in marine ecosystems.It highlights the urgent need develop comprehensive strategies to address these environmental issues.The findings are an important contribution to ecotoxicology,providing a deeper understanding of how microplastics act as vectors for other contaminants and their profound effects on marine life,especially species like seahorses.This study calls for action to strengthen environmental policies and practices aimed at mitigating the impact of marine pollution.
基金supported by the National Key R&D Program of China(No.2021YFC1808902)the National Natural Science Foundation of China(No.31770152)+1 种基金Shaanxi Fundamental Science Research Project for Chemistry&Biology(No.22JHZ008)the Natural Science Foundation of Gansu Province of China(No.23JRRM738).
文摘High molecular weight polycyclic aromatic hydrocarbons(HMW-PAHs)pose significant environmental challenges due to their complex structures and persistent toxicity,and are difficult to be degraded by bacteria.Fungi,however,possess the ability to overcome these challenges,primarily through various enzymes with broad substrate specificity,including cytochrome P450(CYP450),laccase,manganese peroxidase,lignin peroxidase.As a result,the development of efficient fungal strains capable of degrading HMW-PAHs is essential for advancing bioremediation strategies.In this study,Aspergillus fumigatus Z5 was isolated from a contaminated site and demonstrated a remarkable ability to degrade the highly recalcitrant benzo[a]pyrene,achieving a degradation rate of 75.43%.Transcriptomic analysis revealed significant upregulation of 34 CYP450 genes.Among these genes,CYP3A4(gene 8840)showed strong binding affinity for benzo[a]pyrene,as confirmed by molecular docking studies,indicating its key role in the biodegradable process.Furthermore,the metabolic network analysis showed that the electron transfer required for CYP450-mediated oxidative reactions enhanced mitochondrial oxidative phosphorylation and subsequently energy metabolism in A.fumigatus Z5.This metabolic coordination likely facilitates the complete degradation of benzo[a]pyrene.Our study illuminates the fundamental roles of CYP450 from A.fumigatus Z5 in benzo[a]pyrene degradation and provides novel insight into designing and implementing enhanced bioremediation strategies.
基金The project supported by the State Key Develoment Program for Basic Research of China(2003CB716204)the Key Project of the Education Department of China(2005360563)+1 种基金the Key Laboratory of Advanced Photonic and Electronic Materials of Jiangsuthe State Key Laboratory of Solid State Microstructures in Nanjing University.
基金Supported Natural Science Foundation of Guangdong Province(9451064201003780)President Fund in South China Agricultural University(K09219)Students Technology Innovation Project in South China Agricultural University(L09131)~~
文摘[Objective]The paper was to explore the effects of pyrene stress on growth and physiological indexes of maize seedling.[Method] The effects of different concentrations of pyrene（0,0.5,1.0,and 2.0 mg/L） on growth and several physiological indexes,such as chlorophyll content,malnodiadehyde（MDA） content of both leaves and roots of maize（Zea Mays L.） seedlings of Shixiang 1（sensitive variety） and Guangtian 3（tolerant variety） were studied by a hydroponic experiment.[Result]As the concentration of prene increasing within the test concentration range,the contents of chlorophyll a and chlorophyll b in Shixiang 1 first increased and then decreased;relative growth yield and absolute growth rate showed the decreasing trend;MDA contents in roots and leaves showed the increasing trend.Relative growth yield,absolute growth rate and chlorophyll a content in Guangtian 3 first increased and then decreased;chlorophyll b and MDA contents in roots showed the increasing trend;MDA contents in leaves showed the decreasing trend.Compared with control,chlorophyll a and chlorophyll b contents of Shixiang 1 significantly increased under pyrene concentration of 0.5-1.0 mg/L,which significantly decreased as the concentration of pyrene ≥2.0 mg/L;MDA contents in roots and leaves significantly increased as the concentration of pyrene ≥1.0 mg/L,while relative growth yield and absolute growth rate significantly decreased.Chlorophyll a and chlorophyll b contents in Guangtian 3 significantly increased as the concentration of pyrene ≥1.0 mg/L.Pyrene concentration had significantly negative correlation with relative growth yield and absolute growth rate of Shixiang 1,while it had significant positive correlation with MDA contents in roots.Pyrene concentration had significant positive correlation with chlorophyll b and MDA contents in roots of tolerant variety Guangtian 3.[Conclusion]The study provided basis for the management measures of maize during its seedling stage.
基金CGL2014-52599-P “Estudio del manto de nieve enla montana espanola y su respuesta a la variabilidad y cambio climatico” funded by the Spanish Ministry of Economy and CompetitivenessEl glaciar de Monte Perdido: estudio de su dinámica actual y procesos criosféricos asociados como indicadores de procesos de cambio global” (MAGRAMA 844/2013).
文摘This study demonstrated the usefulness of very long-range terrestrial laser scanning(TLS) for analysis of the spatial distribution of a snowpack, to distances up to 3000 m, one of the longest measurement range reported to date. Snow depth data were collected using a terrestrial laser scanner during 11 periods of snow accumulation and melting,over three snow seasons on a Pyrenean hillslopecharacterized by a large elevational gradient, steep slopes, and avalanche occurrence. The maximum and mean absolute snow depth error found was 0.5-0.6 and 0.2-0.3 m respectively, which may result problematic for areas with a shallow snowpack, but it is sufficiently accurate to determine snow distribution patterns in areas characterized by a thick snowpack. The results indicated that in most cases there was temporal consistency in the spatial distribution of thesnowpack, even in different years. The spatial patterns were particularly similar amongst thesurveys conducted during the period dominated by snow accumulation(generally until end of April), or amongst those conducted during the period dominated by melting processes(generally after mid of April or early May). Simple linear correlation analyses for the 11 survey dates, and the application of Random Forests analysis to two days representative of snow accumulation and melting periods indicated the importance of topography to the snow distribution. The results also highlight that elevation and the Topographic Position index(TPI) were the main variables explaining the snow distribution, especially during periods dominated by melting. The intra-and inter-annual spatial consistency of the snowpack distribution suggests that the geomorphological processes linked to presence/absence of snow cover act in a similar way in the long term, and that these spatial patternscan be easily identifiedthrough several years of adequate monitoring.
基金Project Supported by National Natural Science Foundation of China, No. 39870662
文摘AIM To study the inhibitory effects of VES( RRR-α-tocopheryl Succinate, VES ), aderivative of natural Vitamin E, on benzo (a)pyrene (B (a) P)-induced forestomach tumor infemale mice.METHODS The model of B (a)P-inducedforestomach tumor was established according tothe methods of Wattenberg with slightmodifications. One hundred and eighty femalemice (6 weeks old) were divided into six groupsequally; negative control (Succinic acid),vehicle control ( Succinate + B (a) P), positivecontrol(B(a) P), high VES(2.5g/kg. b. w + B(a)P), Iow VES(1 .25 g/kg. b. w + B(a) P) ig as wellas VES by ip (20 mg/kg, b. w + B(a) P). Exceptthe negative control group, the mice wereadministrated with B(a)P ig. and correspondingtreatments for 4 weeks to study the anti-carcinogenetic effect of VES during the initiationperiod. The experiment lasted 29 weeks, inwhich the inhibitory effects of VES both ontumor incidence and tumor size were tested.RESULTS The models of B (a)P-inducedforestomach tumor in female mice wereestablished successfully. Some werecauliflower-like, others looked like papilla, evena few were formed into the ulcer cavities. VES at1.25 g/kg. b. w, 2.5 g/kg. b.w. by ig and 20 mg/kg. b. w. via ip could decrease the number oftumors per mouse (1.7 ± 0. 41, 1.6 ± 0.34 and 1.1±0.43), being lower than that of B(a)P group(5.4 ± 0.32, P＜0.05). The tumor incidence wasinhibited by 18.2%, 23.1% and 50.0%. VES at1.25g/kg.b.w., 2.5 g/ kg.b.w. by ig and20 mg/kg. b.w. via ip reduced the total volumeof tumors per mouse (54.8 ± 8.84, 28.4 ± 8.32and 23.9± 16.05), being significantly lower thanthat of B(a)P group (150.2±20.93, P＜0.01).The inhibitory rates were 63.5%, 81.1% and84.1%, respectively.CONCLUSION VES has inhibitory effects on B(a) P-induced forestomach carcinogenesis infemale mice, especially by ip and it may be apotential anti-cancer agent in vivo.
基金The National Basic Research Program (973) of China (No. 2004CB418506)the National Natural Science Foundation of China (No.20337010) the Hi-Tech Research and Development Program (863) of China (No. 2004AA649060)
文摘Two indigenous microorganisms, Bacillus sp. SB02 and Mucor sp. SF06, capable of degrading polycyclic aromatic hydrocarbons （PAHs） were co-immobilized on vermiculite by physical adsorption and used to degrade benzo[a] pyrene （BaP）. The characteristics of BaP degradation by both free and co-immobilized microorganism were then investigated and compared. The removal rate using the immobilized bacterial-fungal mixed consortium was higher than that of the freely mobile mixed consortium. 95.3% of BaP was degraded using the co-immobilized system within 42 d, which was remarkably higher than the removal rate of that by the free strains. The optimal amount of inoculated co-immobilized system for BaP degradation was 2%. The immobilized bacterial-fungal mixed consortium also showed better water stability than the free strains. Kinetics of BaP biodegradation by co-immobilized SF06 and SB02 were also studied. The results demonstrated that BaP degradation could be well described by a zero-order reaction rate equation when the initial BaP concentration was in the range of 10--200 mg/kg. The scanning electronic microscope （SEM） analysis showed that the co-immobilized microstructure was suitable for the growth of SF06 and SB02. The mass transmission process of co-immobilized system in soil is discussed. The results demonstrate the potential for employing the bacterial-fungal mixed consortium, co-immobilized on vermiculite, for in situ bioremediation of BaP.
