Abscisic acid(ABA)is a phytohormone that not only important for plant growth,but also mediating the stress response.The roles of ABA in plant immunity are especially multifaceted.Recently,the ABA functional analogues ...Abscisic acid(ABA)is a phytohormone that not only important for plant growth,but also mediating the stress response.The roles of ABA in plant immunity are especially multifaceted.Recently,the ABA functional analogues are of great significance to promote its application.Here,we reported an ABA functional analogue named 167A.167A inhibits plant growth and seeds germinating of Arabidopsis.Meanwhile,the 167A enhanced the plant immunity,which is opposite of ABA.We further investigated the PTI-response after 167A treatment,and the results show that the ROS burst,callose deposition accumulate with 167A treatment.Moreover,167A also influence the degree of stomal closed.RNA-seq assays show that the 167A down-regulated the ABA associated genes and upregulated the JA/SA/ET associated genes.Through genetic analysis,the 167A modulating the plant resistance through the PYR/PYL Receptors.Together,these results demonstrate that a novel ABA analogue 167A positive regulated plant immunity and has great potential for agricultural applications.展开更多
As abscisic acid(ABA)receptor,the pyrabactin resistance 1-like(PYR/PYL)protein(named PYL for simplicity)plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms.Glycyrrhiza uralen...As abscisic acid(ABA)receptor,the pyrabactin resistance 1-like(PYR/PYL)protein(named PYL for simplicity)plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms.Glycyrrhiza uralensis,a drought-tolerant medicinal plant,is a good model for the mechanism analysis of ABA response and active compound biosynthesis.However,knowledge about PYL family in G.uralensis remains largely unknown.Here,10 PYLs were identified in G.uralensis genome.Characterization analysis indicated that PYLs in G.uralensis(Gu PYLs)are relatively conserved.Phylogenetic analysis showed that Gu PYL1-3 belongs to subfamily I,Gu PYL4-6 and Gu PYL10 belong to subfamily II and Gu PYL7-9 belongs to subfamily III.In addition,transcriptome data presented various expression levels of Gu PYLs under different exogenous ABA stresses.The expression pattern of Gu PYLs was verified by Quantitative real-time polymerase chain reaction(q RT-PCR).The study proved that Gu PYL4,Gu PYL5,Gu PYL8 and Gu PYL9 genes are significantly up-regulated by ABA stress and the response process is dynamic.This study paves the way for elucidating the regulation mechanism of ABA signal to secondary metabolites and improving the cultivation and quality of G.uralensis using agricultural strategies.展开更多
Seed germination and seedling establishment are important for the reproductive success of plants,but seeds and seedlings typically encounter constantly changing environmental conditions.By inhibiting seed germination ...Seed germination and seedling establishment are important for the reproductive success of plants,but seeds and seedlings typically encounter constantly changing environmental conditions.By inhibiting seed germination and post-germinative growth through the PYR1/PYL/RCAR ABA receptors and PP2C co-receptors,the phytohormone abscisic acid(ABA)prevents premature germination and seedling growth under unfavorable conditions.However,little is known about how the ABA-mediated inhibition of seed germination and seedling establishment is thwarted.Here,we report that ABA Signaling Terminator(ABT),a WD40 protein,efficiently switches off ABA signaling and is critical for seed germination and seedling establishment.ABT is induced by ABA in a PYR1/PYL/RCAR-PP2C-dependent manner.Overexpression of ABT promotes seed germination and seedling greening in the presence of ABA,whereas knockout of ABT has the opposite effect.We found that ABT interacts with the PYR1/PYL/RCAR and PP2C proteins,interferes with the interaction between PYR1/PYL4 and ABI1/ABI2,and hampers the inhibition of ABI1/ABI2 by ABA-bound PYR1/PYL4,thereby terminating ABA signaling.Taken together,our results reveal a core mechanism of ABA signaling termination that is critical for seed germination and seedling establishment in Arabidopsis.展开更多
基金supported by the National Key Research and Development Program(2022YFD1402100,2022YFD1401500)National Natural Science Foundation(32072500,32272557)+1 种基金Major Basic Research Project of Natural Science Foundation of Shandong Province(ZR2022ZD23)Shandong Province Key Research and Development Plan(2022TZXD0025,2021TZXD007-04-4),Taishan Scholar Program of Shandong Province.
文摘Abscisic acid(ABA)is a phytohormone that not only important for plant growth,but also mediating the stress response.The roles of ABA in plant immunity are especially multifaceted.Recently,the ABA functional analogues are of great significance to promote its application.Here,we reported an ABA functional analogue named 167A.167A inhibits plant growth and seeds germinating of Arabidopsis.Meanwhile,the 167A enhanced the plant immunity,which is opposite of ABA.We further investigated the PTI-response after 167A treatment,and the results show that the ROS burst,callose deposition accumulate with 167A treatment.Moreover,167A also influence the degree of stomal closed.RNA-seq assays show that the 167A down-regulated the ABA associated genes and upregulated the JA/SA/ET associated genes.Through genetic analysis,the 167A modulating the plant resistance through the PYR/PYL Receptors.Together,these results demonstrate that a novel ABA analogue 167A positive regulated plant immunity and has great potential for agricultural applications.
基金supported by the National Science and Technology Major Project for“Significant New Drugs Development”(No.2019ZX09201005-006-003)the ChinesAcademy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(CIFMS)(No.2016-I2M-3-016)。
文摘As abscisic acid(ABA)receptor,the pyrabactin resistance 1-like(PYR/PYL)protein(named PYL for simplicity)plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms.Glycyrrhiza uralensis,a drought-tolerant medicinal plant,is a good model for the mechanism analysis of ABA response and active compound biosynthesis.However,knowledge about PYL family in G.uralensis remains largely unknown.Here,10 PYLs were identified in G.uralensis genome.Characterization analysis indicated that PYLs in G.uralensis(Gu PYLs)are relatively conserved.Phylogenetic analysis showed that Gu PYL1-3 belongs to subfamily I,Gu PYL4-6 and Gu PYL10 belong to subfamily II and Gu PYL7-9 belongs to subfamily III.In addition,transcriptome data presented various expression levels of Gu PYLs under different exogenous ABA stresses.The expression pattern of Gu PYLs was verified by Quantitative real-time polymerase chain reaction(q RT-PCR).The study proved that Gu PYL4,Gu PYL5,Gu PYL8 and Gu PYL9 genes are significantly up-regulated by ABA stress and the response process is dynamic.This study paves the way for elucidating the regulation mechanism of ABA signal to secondary metabolites and improving the cultivation and quality of G.uralensis using agricultural strategies.
基金supported by the National Key Research and Development Program of China(2016YFA0500503)the Fundamental Research Funds for the Central Universities(2662018PY075)+1 种基金the National Natural Science Foundation of China(31730066,91540112)the Huazhong Agricultural University's Scientific and Technological Self-innovation Foundation(2015RC014).
文摘Seed germination and seedling establishment are important for the reproductive success of plants,but seeds and seedlings typically encounter constantly changing environmental conditions.By inhibiting seed germination and post-germinative growth through the PYR1/PYL/RCAR ABA receptors and PP2C co-receptors,the phytohormone abscisic acid(ABA)prevents premature germination and seedling growth under unfavorable conditions.However,little is known about how the ABA-mediated inhibition of seed germination and seedling establishment is thwarted.Here,we report that ABA Signaling Terminator(ABT),a WD40 protein,efficiently switches off ABA signaling and is critical for seed germination and seedling establishment.ABT is induced by ABA in a PYR1/PYL/RCAR-PP2C-dependent manner.Overexpression of ABT promotes seed germination and seedling greening in the presence of ABA,whereas knockout of ABT has the opposite effect.We found that ABT interacts with the PYR1/PYL/RCAR and PP2C proteins,interferes with the interaction between PYR1/PYL4 and ABI1/ABI2,and hampers the inhibition of ABI1/ABI2 by ABA-bound PYR1/PYL4,thereby terminating ABA signaling.Taken together,our results reveal a core mechanism of ABA signaling termination that is critical for seed germination and seedling establishment in Arabidopsis.
