Postbiotics are preparations made from inanimate microorganisms and/or their components that are beneficial to host health,but the underlying specific modes of action in relieving intestinal inflammation remain poorly...Postbiotics are preparations made from inanimate microorganisms and/or their components that are beneficial to host health,but the underlying specific modes of action in relieving intestinal inflammation remain poorly understood.This study aims to investigate the anti-inflammatory activity,intestinal barrier recovery,antioxidant activity,and correction of microbial dysbiosis of PWE(postbiotics water extract),a novel postbiotics derived from Bacillus amyloliquefaciens J and Lactobacillus plantarum SN4 named PWE.In this study,C57BL/6 male mice were administered PWE by oral gavage for seven days,followed by a three-day K88 challenge to evaluate its protective effects against enteritis.Our results showed that PWE exerted an obvious protective effect on ETECchallenged enteritis mice.Specifically,PWE improved overall health status and colon length,restored jejunal and colonic villi and crypt morphology,and reduced inflammatory cell infiltration.Moreover,PWE enhanced the expression of tight junction proteins,including Occludin,and upregulated the mRNA expression of ZO-1 and Claudin,modulated apoptosis-related genes(BAX,Bcl2,and Caspase-3),and strengthened liver antioxidant capacity.Gut microbiota analysis revealed that PWE rebalanced the microbial composition through decreasing Escherichia-Shigella abundance and increasing Lactobacillaceae and Lachnospiraceae abundance that promote SCFA production.Furthermore,correlation analysis demonstrated that these increases in abundance of Lactobacillaceae were associated with reduced intestinal inflammation and improved barrier function.Abundance of Ligilactobacillus was positively correlated to anti-apoptotic effects and antioxidant properties.In conclusion,our findings highlight the modulatory effects of the postbiotics PWE in alleviating intestinal inflammation,repairing barrier dysfunction,and restoring microbial imbalance through microbiota regulation and SCFA enhancement.These results provide new insights and strategies for combating bacterial infections diarrhea and improving intestinal health.展开更多
目前以航空航天为代表的极端环境领域对板结构的静态和动态力学性质都提出了更高的要求。在保证结构承载能力的同时,还需要实现轻质化以及使其具有多频带减隔振功能。针对上述要求,设计了一种拉扭耦合超结构板,利用由两个自由度耦合谐...目前以航空航天为代表的极端环境领域对板结构的静态和动态力学性质都提出了更高的要求。在保证结构承载能力的同时,还需要实现轻质化以及使其具有多频带减隔振功能。针对上述要求,设计了一种拉扭耦合超结构板,利用由两个自由度耦合谐振产生的两个低频弯曲波带隙,实现超结构板的低频双频带减振。首先提出一种具有拉扭双自由度的单胞等效模型,并在此基础上利用平面波展开(Plane Wave Expansion,PWE)法进行超结构板带隙特性的计算,最后通过有限元分析(Finite Element Analysis,FEA)验证了带隙计算方法的正确性。研究发现,相比于拉压平动单自由度谐振子,拉扭耦合双自由度谐振子会在板结构中打开一个新的弯曲波带隙。进一步分析归一化等效刚度系数对两个带隙位置和带宽的影响。最后,利用有限元方法计算10'5阵列超结构板的振动传递率,得出的振动衰减区域与由理论计算所得的带隙位置一致。有关结论可为轻质超结构板的多频带减振设计提供参考。展开更多
随着电力通信业务IP化趋势的不断发展,分组传送网(Packet Transfer Network,PTN)逐渐被广泛采用,但是对于分组传送网对E1业务的承载性能,目前仍存在较大争议。文章以分析、评估PTN网络对E1业务的承载能力为出发点,通过原理分析、实验室...随着电力通信业务IP化趋势的不断发展,分组传送网(Packet Transfer Network,PTN)逐渐被广泛采用,但是对于分组传送网对E1业务的承载性能,目前仍存在较大争议。文章以分析、评估PTN网络对E1业务的承载能力为出发点,通过原理分析、实验室测试等方式,验证了PTN网络对E1业务的优良承载性能,为未来电力通信网络建设和工程设计提供了新的参考和可靠的依据。展开更多
基金supported by the National Key R&D projects with No.2022YFD1300900the Beijing Science and Technology Plan with No.Z201100008020018.
文摘Postbiotics are preparations made from inanimate microorganisms and/or their components that are beneficial to host health,but the underlying specific modes of action in relieving intestinal inflammation remain poorly understood.This study aims to investigate the anti-inflammatory activity,intestinal barrier recovery,antioxidant activity,and correction of microbial dysbiosis of PWE(postbiotics water extract),a novel postbiotics derived from Bacillus amyloliquefaciens J and Lactobacillus plantarum SN4 named PWE.In this study,C57BL/6 male mice were administered PWE by oral gavage for seven days,followed by a three-day K88 challenge to evaluate its protective effects against enteritis.Our results showed that PWE exerted an obvious protective effect on ETECchallenged enteritis mice.Specifically,PWE improved overall health status and colon length,restored jejunal and colonic villi and crypt morphology,and reduced inflammatory cell infiltration.Moreover,PWE enhanced the expression of tight junction proteins,including Occludin,and upregulated the mRNA expression of ZO-1 and Claudin,modulated apoptosis-related genes(BAX,Bcl2,and Caspase-3),and strengthened liver antioxidant capacity.Gut microbiota analysis revealed that PWE rebalanced the microbial composition through decreasing Escherichia-Shigella abundance and increasing Lactobacillaceae and Lachnospiraceae abundance that promote SCFA production.Furthermore,correlation analysis demonstrated that these increases in abundance of Lactobacillaceae were associated with reduced intestinal inflammation and improved barrier function.Abundance of Ligilactobacillus was positively correlated to anti-apoptotic effects and antioxidant properties.In conclusion,our findings highlight the modulatory effects of the postbiotics PWE in alleviating intestinal inflammation,repairing barrier dysfunction,and restoring microbial imbalance through microbiota regulation and SCFA enhancement.These results provide new insights and strategies for combating bacterial infections diarrhea and improving intestinal health.
文摘目前以航空航天为代表的极端环境领域对板结构的静态和动态力学性质都提出了更高的要求。在保证结构承载能力的同时,还需要实现轻质化以及使其具有多频带减隔振功能。针对上述要求,设计了一种拉扭耦合超结构板,利用由两个自由度耦合谐振产生的两个低频弯曲波带隙,实现超结构板的低频双频带减振。首先提出一种具有拉扭双自由度的单胞等效模型,并在此基础上利用平面波展开(Plane Wave Expansion,PWE)法进行超结构板带隙特性的计算,最后通过有限元分析(Finite Element Analysis,FEA)验证了带隙计算方法的正确性。研究发现,相比于拉压平动单自由度谐振子,拉扭耦合双自由度谐振子会在板结构中打开一个新的弯曲波带隙。进一步分析归一化等效刚度系数对两个带隙位置和带宽的影响。最后,利用有限元方法计算10'5阵列超结构板的振动传递率,得出的振动衰减区域与由理论计算所得的带隙位置一致。有关结论可为轻质超结构板的多频带减振设计提供参考。
文摘随着电力通信业务IP化趋势的不断发展,分组传送网(Packet Transfer Network,PTN)逐渐被广泛采用,但是对于分组传送网对E1业务的承载性能,目前仍存在较大争议。文章以分析、评估PTN网络对E1业务的承载能力为出发点,通过原理分析、实验室测试等方式,验证了PTN网络对E1业务的优良承载性能,为未来电力通信网络建设和工程设计提供了新的参考和可靠的依据。
