Objective Sepsis,a life-threatening condition caused by a dysregulated host response to infection,continues to be a major cause of mortality in critical care despite medical advancements.This study aimed to investigat...Objective Sepsis,a life-threatening condition caused by a dysregulated host response to infection,continues to be a major cause of mortality in critical care despite medical advancements.This study aimed to investigate the therapeutic effects and neuroimmune mechanisms of electroacupuncture(EA)at the Shenshu(BL23)acupoint combined with antibiotic therapy in sepsis management.Methods A cecal ligation and puncture(CLP)-induced murine sepsis model was used to evaluate the combined therapy.The study employed enzyme-linked immunosorbent assays(ELISA)and histological analysis to assess systemic inflammation and intestinal damage.Three-dimensional immunolabeling of c-Fos neurons and chemogenetic modulation of hypothalamic paraventricular nucleus(PVN)neurons were performed to investigate neural mechanisms.Additionally,serum catecholamine levels were measured using ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Results The combination of EA and antibiotics significantly improved survival rates and reduced sepsis-induced weight loss.The therapy lowered systemic levels of proinflammatory cytokines(TNF-αand IL-6)and mitigated intestinal inflammatory infiltration.EA at BL23 suppressed neuronal activation in the PVN by reducing c-Fos expression.Chemogenetic inhibition of corticotropin-releasing hormone(CRH)neurons replicated the anti-inflammatory effects of the therapy,while their activation diminished therapeutic benefits.Conversely,activation of oxytocin(OXT)neurons reproduced the anti-inflammatory effects,and their inhibition reversed these benefits.The combined therapy also elevated serum catecholamine levels,indicating sympathetic-mediated immunomodulation.Conclusion This study demonstrates that EA at BL23 enhances antibiotic efficacy in sepsis by modulating PVN activity—suppressing CRH neurons and activating OXT neurons—leading to increased catecholamine secretion and systemic inflammation control.These findings reveal a novel neuroimmunological pathway for acupuncture’s therapeutic role in sepsis,supporting its potential as a noninvasive adjunctive therapy in critical care.展开更多
A better understanding is needed to explain the mechanism of therapeutic effect of combined use of tetradrine-PVNO and tetradrine-QOHP which play very important roles in treatment of silicosis. Blood prolidase (PLD), ...A better understanding is needed to explain the mechanism of therapeutic effect of combined use of tetradrine-PVNO and tetradrine-QOHP which play very important roles in treatment of silicosis. Blood prolidase (PLD), monoamine oxidase (MAO) and plasminogen (PLG) in silicotic rats after treatment with tetradrine-PVNO or tetradrine-QOHP were measured. The values obtained were compared with the untreated silicotic rats. It was found that the silicotic rats that received tetradrine-PVNO showed significant increase in PLD and decrease in PLG, but no significant change in MAO. The PLD in plasma of silicotic rats that received tetradrine-QOHP were elevated significantly, but PLG and MAO did not change appreciably. These findings suggest that the combined use of tetradrine-PVNO and tetradrine-QOHP can accelerate the degradation of collagen in silicotic rats展开更多
Objective: To observe the influence of electrolytic destruction of nucleus soli tary tract (NTS) and hypothalamic paraventricular nucleus (PVN) on the effect of electroacupuncture (EA) in improving ischemic myocardia ...Objective: To observe the influence of electrolytic destruction of nucleus soli tary tract (NTS) and hypothalamic paraventricular nucleus (PVN) on the effect of electroacupuncture (EA) in improving ischemic myocardia cellular transmembrane action potential (TMAP). Methods: 38 Japanese breed big-ear wh ite rabbits (ane sthetized with 20% Urethane, 4 mL/kg) were randomly divided into acute myocardia l ischemia (AMI) group (n=8), PVN destruction group ( n=12) and PVN+NTS destructio n group (n=18). AMI model was established by occlusion of the descending anterio r branch (DAB) of the coronary artery. TMAP of myocytes was recorded by using a glass microelectrode which was fixed to a suspending spring silver wire. Bilater al "Neiguan"(PC 6) in all the 3 groups were punctured and stimulated electri call y by using parameters of continuous waves, frequency of 7 Hz, intensity of 6 mA a nd duration of 30 minutes. Results: After AMI, ECG-ST elevated significantly whil e APA lowered, APD50 and APD90 shortened clearly in comparison with those of pre -AMI in the 3 groups. Compared with AMI group, ECG-ST values of PVN destructi on group and PVN+NTS destruction group were significantly higher (P <0.05～0.01), whi le APA, APD50 and APD90 all significantly lower in all the recording time course s(P<0.05). The facts displayed that electrolytic destru ction of PVN and PVN+NT S could produce ischemic myocardial injury and reduce the protective effect of E A on ischemic myocardial cells. Comparison between PVN destruction and PVN+NTS g roups showed that all the 4 indexes of the later group were evidently worse than those of the former group (P<0.05), suggesting after des truction of these two n uclei, the effect of EA was worsened further. Conclusion: Electrolytic destru ction of PVN and NTS weakens the protective effect of EA on ischemic myocardial cells, both NTS and PVN take part in the effect of EA of "Neiguan"(PC 6) Point i n improving ischemic myocardium.展开更多
基金the National Key Research and Development Program of China(2022YFC3500704).
文摘Objective Sepsis,a life-threatening condition caused by a dysregulated host response to infection,continues to be a major cause of mortality in critical care despite medical advancements.This study aimed to investigate the therapeutic effects and neuroimmune mechanisms of electroacupuncture(EA)at the Shenshu(BL23)acupoint combined with antibiotic therapy in sepsis management.Methods A cecal ligation and puncture(CLP)-induced murine sepsis model was used to evaluate the combined therapy.The study employed enzyme-linked immunosorbent assays(ELISA)and histological analysis to assess systemic inflammation and intestinal damage.Three-dimensional immunolabeling of c-Fos neurons and chemogenetic modulation of hypothalamic paraventricular nucleus(PVN)neurons were performed to investigate neural mechanisms.Additionally,serum catecholamine levels were measured using ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Results The combination of EA and antibiotics significantly improved survival rates and reduced sepsis-induced weight loss.The therapy lowered systemic levels of proinflammatory cytokines(TNF-αand IL-6)and mitigated intestinal inflammatory infiltration.EA at BL23 suppressed neuronal activation in the PVN by reducing c-Fos expression.Chemogenetic inhibition of corticotropin-releasing hormone(CRH)neurons replicated the anti-inflammatory effects of the therapy,while their activation diminished therapeutic benefits.Conversely,activation of oxytocin(OXT)neurons reproduced the anti-inflammatory effects,and their inhibition reversed these benefits.The combined therapy also elevated serum catecholamine levels,indicating sympathetic-mediated immunomodulation.Conclusion This study demonstrates that EA at BL23 enhances antibiotic efficacy in sepsis by modulating PVN activity—suppressing CRH neurons and activating OXT neurons—leading to increased catecholamine secretion and systemic inflammation control.These findings reveal a novel neuroimmunological pathway for acupuncture’s therapeutic role in sepsis,supporting its potential as a noninvasive adjunctive therapy in critical care.
文摘A better understanding is needed to explain the mechanism of therapeutic effect of combined use of tetradrine-PVNO and tetradrine-QOHP which play very important roles in treatment of silicosis. Blood prolidase (PLD), monoamine oxidase (MAO) and plasminogen (PLG) in silicotic rats after treatment with tetradrine-PVNO or tetradrine-QOHP were measured. The values obtained were compared with the untreated silicotic rats. It was found that the silicotic rats that received tetradrine-PVNO showed significant increase in PLD and decrease in PLG, but no significant change in MAO. The PLD in plasma of silicotic rats that received tetradrine-QOHP were elevated significantly, but PLG and MAO did not change appreciably. These findings suggest that the combined use of tetradrine-PVNO and tetradrine-QOHP can accelerate the degradation of collagen in silicotic rats
基金This studyis subsidized by National Science Foundation of China (No .30171179) .
文摘Objective: To observe the influence of electrolytic destruction of nucleus soli tary tract (NTS) and hypothalamic paraventricular nucleus (PVN) on the effect of electroacupuncture (EA) in improving ischemic myocardia cellular transmembrane action potential (TMAP). Methods: 38 Japanese breed big-ear wh ite rabbits (ane sthetized with 20% Urethane, 4 mL/kg) were randomly divided into acute myocardia l ischemia (AMI) group (n=8), PVN destruction group ( n=12) and PVN+NTS destructio n group (n=18). AMI model was established by occlusion of the descending anterio r branch (DAB) of the coronary artery. TMAP of myocytes was recorded by using a glass microelectrode which was fixed to a suspending spring silver wire. Bilater al "Neiguan"(PC 6) in all the 3 groups were punctured and stimulated electri call y by using parameters of continuous waves, frequency of 7 Hz, intensity of 6 mA a nd duration of 30 minutes. Results: After AMI, ECG-ST elevated significantly whil e APA lowered, APD50 and APD90 shortened clearly in comparison with those of pre -AMI in the 3 groups. Compared with AMI group, ECG-ST values of PVN destructi on group and PVN+NTS destruction group were significantly higher (P <0.05～0.01), whi le APA, APD50 and APD90 all significantly lower in all the recording time course s(P<0.05). The facts displayed that electrolytic destru ction of PVN and PVN+NT S could produce ischemic myocardial injury and reduce the protective effect of E A on ischemic myocardial cells. Comparison between PVN destruction and PVN+NTS g roups showed that all the 4 indexes of the later group were evidently worse than those of the former group (P<0.05), suggesting after des truction of these two n uclei, the effect of EA was worsened further. Conclusion: Electrolytic destru ction of PVN and NTS weakens the protective effect of EA on ischemic myocardial cells, both NTS and PVN take part in the effect of EA of "Neiguan"(PC 6) Point i n improving ischemic myocardium.
