The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on the dopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densities for the growth of DA neuron culture...The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on the dopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densities for the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as com- pared to those without CPe(p<0.05).Rhodamine retrograde-prelabelled DA neurons were cultured on a Phastgel containing all the electrophoretically separated protein bands from CPe,and able to fish out from it their own trophic factor,a 12.5-kD protein band.The survived neurons at the 12.5-kD protein band were immunostained positive with anti-dopamine antibody.Co-culturing the 12.5 kD-containing gel strip with ex- plants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neurites outgrowing from the explants only towards the 12.5-kD gel strip.These results indicate that a 12.5-kD pro- tein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promoting their neurite outgrowth.展开更多
基金the University of Hong Kong and the Growth Factor
文摘The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on the dopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densities for the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as com- pared to those without CPe(p<0.05).Rhodamine retrograde-prelabelled DA neurons were cultured on a Phastgel containing all the electrophoretically separated protein bands from CPe,and able to fish out from it their own trophic factor,a 12.5-kD protein band.The survived neurons at the 12.5-kD protein band were immunostained positive with anti-dopamine antibody.Co-culturing the 12.5 kD-containing gel strip with ex- plants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neurites outgrowing from the explants only towards the 12.5-kD gel strip.These results indicate that a 12.5-kD pro- tein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promoting their neurite outgrowth.
