目的探究PTTG1基因表达与肝细胞癌的相关性。方法基于癌症基因组图谱(The Cancer Genome Atlsa,TCGA)数据库分析PTTG1基因的mRNA表达水平及其与肝细胞癌患者临床病理特征的相关性;基于Kaplan-Meier Plotter数据库研究PTTG1基因表达与肝...目的探究PTTG1基因表达与肝细胞癌的相关性。方法基于癌症基因组图谱(The Cancer Genome Atlsa,TCGA)数据库分析PTTG1基因的mRNA表达水平及其与肝细胞癌患者临床病理特征的相关性;基于Kaplan-Meier Plotter数据库研究PTTG1基因表达与肝细胞癌患者生存之间的关系;借助STRING数据库构建PTTG1及其相互作用因子交互网络;基于LinkedOmics平台挖掘PTTG1在肝细胞癌中的共表达网络,并进行基因本体论(Gene Ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路分析;利用c-BioPortal平台结合TCGA数据库分析PTTG1在肝细胞癌中的基因变异情况。采用R 4.4.1软件进行统计分析与数据可视化。结果PTTG1基因在肝细胞癌组织中的表达水平显著升高,且存在变异,其表达水平与肝细胞癌患者的总体生存期相关(P<0.05)。在PTTG1相互作用网络节点中,ESPL1和PTTG2的节点度更高。在肝细胞癌中,PTTG1主要参与调控细胞周期的转换、物质代谢等。结论PTTG1基因的表达、变异及其调控机制可能与肝细胞癌的发生发展及其预后具有一定的关联性,可为深入探究PTTG1基因在肝细胞癌中的功能角色提供参考。展开更多
BACKGROUND Few studies have investigated the expression of GLI1 and PTTG1 in patients undergoing radical surgery for colorectal carcinoma(CRC)and their association with lymph node metastasis(LNM).Therefore,more releva...BACKGROUND Few studies have investigated the expression of GLI1 and PTTG1 in patients undergoing radical surgery for colorectal carcinoma(CRC)and their association with lymph node metastasis(LNM).Therefore,more relevant studies and analyses need to be conducted.AIM To explore GLI1 and PTTG1 expression in patients undergoing radical surgery for CRC and their correlation with LNM.METHODS This study selected 103 patients with CRC admitted to our hospital between April 2020 and April 2023.Sample specimens of CRC and adjacent tissues were collected to determine the positive rates and expression levels of GLI1 and PTTG1.The correlation of the two genes with patients’clinicopathological data(e.g.,LNM)was explored,and differences in GLI1 and PTTG1 expression between patients with LNM and those without were analyzed.Receiver operating characteristic(ROC)curves were plotted to evaluate the predictive potential of the two genes for LNM in patients with CRC.RESULTS Significantly higher positive rates and expression levels of GLI1 and PTTG1 wereobserved in CRC tissue samples compared with adjacent tissues.GLI1 and PTTG1 were strongly linked to LNM in patients undergoing radical surgery for CRC,with higher GLI1 and PTTG1 levels found in patients with LNM than in those without.The areas under the ROC curve of GLI1 and PTTG1 in assessing LNM in patients with CRC were 0.824 and 0.811,respectively.CONCLUSION GLI1 and PTTG1 expression was upregulated in patients undergoing radical surgery for CRC and are significantly related to LNM in these patients.Moreover,high GLI1 and PTTG1 expression can indicate LNM in patients with CRC undergoing radical surgery.The expression of both genes has certain diagnostic and therapeutic significance.展开更多
文摘目的探究PTTG1基因表达与肝细胞癌的相关性。方法基于癌症基因组图谱(The Cancer Genome Atlsa,TCGA)数据库分析PTTG1基因的mRNA表达水平及其与肝细胞癌患者临床病理特征的相关性;基于Kaplan-Meier Plotter数据库研究PTTG1基因表达与肝细胞癌患者生存之间的关系;借助STRING数据库构建PTTG1及其相互作用因子交互网络;基于LinkedOmics平台挖掘PTTG1在肝细胞癌中的共表达网络,并进行基因本体论(Gene Ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路分析;利用c-BioPortal平台结合TCGA数据库分析PTTG1在肝细胞癌中的基因变异情况。采用R 4.4.1软件进行统计分析与数据可视化。结果PTTG1基因在肝细胞癌组织中的表达水平显著升高,且存在变异,其表达水平与肝细胞癌患者的总体生存期相关(P<0.05)。在PTTG1相互作用网络节点中,ESPL1和PTTG2的节点度更高。在肝细胞癌中,PTTG1主要参与调控细胞周期的转换、物质代谢等。结论PTTG1基因的表达、变异及其调控机制可能与肝细胞癌的发生发展及其预后具有一定的关联性,可为深入探究PTTG1基因在肝细胞癌中的功能角色提供参考。
文摘BACKGROUND Few studies have investigated the expression of GLI1 and PTTG1 in patients undergoing radical surgery for colorectal carcinoma(CRC)and their association with lymph node metastasis(LNM).Therefore,more relevant studies and analyses need to be conducted.AIM To explore GLI1 and PTTG1 expression in patients undergoing radical surgery for CRC and their correlation with LNM.METHODS This study selected 103 patients with CRC admitted to our hospital between April 2020 and April 2023.Sample specimens of CRC and adjacent tissues were collected to determine the positive rates and expression levels of GLI1 and PTTG1.The correlation of the two genes with patients’clinicopathological data(e.g.,LNM)was explored,and differences in GLI1 and PTTG1 expression between patients with LNM and those without were analyzed.Receiver operating characteristic(ROC)curves were plotted to evaluate the predictive potential of the two genes for LNM in patients with CRC.RESULTS Significantly higher positive rates and expression levels of GLI1 and PTTG1 wereobserved in CRC tissue samples compared with adjacent tissues.GLI1 and PTTG1 were strongly linked to LNM in patients undergoing radical surgery for CRC,with higher GLI1 and PTTG1 levels found in patients with LNM than in those without.The areas under the ROC curve of GLI1 and PTTG1 in assessing LNM in patients with CRC were 0.824 and 0.811,respectively.CONCLUSION GLI1 and PTTG1 expression was upregulated in patients undergoing radical surgery for CRC and are significantly related to LNM in these patients.Moreover,high GLI1 and PTTG1 expression can indicate LNM in patients with CRC undergoing radical surgery.The expression of both genes has certain diagnostic and therapeutic significance.
文摘目的 阐明垂体瘤转化基因(PTTG1)与肾透明细胞癌(KIRC)预后及肿瘤免疫细胞浸润的关系。方法 用TCGA数据库获取KIRC样本的相关数据;分析PTTG1在KIRC中的表达水平及临床分期和预后的相关性;用qPCR检测KIRC细胞和肾小管上皮细胞中PTTG1的表达水平;在The Human Protein Atlas数据库中分析PTTG1的蛋白表达水平;分析PTTG1基因和肿瘤免疫浸润的关系;用GSEA进行通路富集分析。结果 KIRC组织中PTTG1 mRNA的转录水平较正常组织显著升高(P<0.01),具有较高的诊断价值。PTTG1与TNM分期和病理分期均存在相关性。PTTG1高表达组的患者总生存期(OS)、疾病特异生存率(DSS)和无进展间隔期(PFS)均显著短于低表达组(P<0.01),PTTG1高表达是影响患者预后的独立危险因素。PTTG1基因表达与Th2、Treg和Th1细胞的浸润程度呈显著正相关(P<0.01)。GSEA结果证实PTTG1主要参与细胞周期及免疫相关的信号通路。结论 PTTG1有望成为KIRC潜在的诊断和预后生物标志物及免疫治疗靶点。
