联合运用NF-κB小分子抑制剂小白菊内酯(parthenolide,PTL)和携带TRAIL的溶瘤腺病毒ZD55-TRAIL在体外杀伤人肝癌细胞株Hep3B,以评估两者联用的实验疗效。首先用Adeasy系统包装ZD55-TRAIL病毒;分别用MTT、结晶紫和Hoechst33342荧光染色...联合运用NF-κB小分子抑制剂小白菊内酯(parthenolide,PTL)和携带TRAIL的溶瘤腺病毒ZD55-TRAIL在体外杀伤人肝癌细胞株Hep3B,以评估两者联用的实验疗效。首先用Adeasy系统包装ZD55-TRAIL病毒;分别用MTT、结晶紫和Hoechst33342荧光染色实验检测ZD55-TRAIL、ZD55-TRAIL和PTL联合运用对肝癌细胞株Hep3B和正常肝细胞QSG-7701的增殖抑制作用。结果表明:10 MOI ZD55-TRAIL联合PTL处理72 h后,Hep3B细胞的存活率仅为44%,但对QSG-7701细胞无明显损伤;而10 MOI的ZD55-TRAIL和PTL分别单独处理后细胞存活率为70%和66%;结晶紫实验及Hoechst33342染色结果表明联合处理组的Hep3B细胞凋亡特征更加明显。溶瘤腺病毒ZD55-TRAIL联合PTL在体外对肝癌细胞Hep3B具有显著的杀伤性,而对QSG-7701细胞无明显毒副作用。展开更多
While unitized regenerative fuel cells(URFCs)are promising for renewable energy storage,their efficient operation requires simultaneous water management and gas transport,which is challenging from the standpoint of wa...While unitized regenerative fuel cells(URFCs)are promising for renewable energy storage,their efficient operation requires simultaneous water management and gas transport,which is challenging from the standpoint of water management.Herein,a novel approach is introduced for examining the alignment hydrophilic pattern of a Ti porous transport layer(PTL)with the flow field of a bipolar plate(BP).UV/ozone patterning and is employed to impart amphiphilic characteristics to the hydrophobic silanized Ti PTL,enabling low-cost and scalable fabrication.The hydrophilic pattern and its alignment with the BP are comprehensively analyzed using electrochemical methods and computational simulations.Notably,the serpentine-patterned(SP)Ti PTL,wherein the hydrophilic channel is directly aligned with the serpentine flow field of the BP,effectively enhances oxygen removal in the water electrolyzer(WE)mode and mitigates water flooding in the fuel cell(FC)mode,ensuring uninterrupted water and gas flow.Further,URFCs with SP configuration exhibit remarkable performance in the WE and FC modes,achieving a significantly improved round-trip efficiency of 25.7%at 2 A cm^(−2).展开更多
文摘联合运用NF-κB小分子抑制剂小白菊内酯(parthenolide,PTL)和携带TRAIL的溶瘤腺病毒ZD55-TRAIL在体外杀伤人肝癌细胞株Hep3B,以评估两者联用的实验疗效。首先用Adeasy系统包装ZD55-TRAIL病毒;分别用MTT、结晶紫和Hoechst33342荧光染色实验检测ZD55-TRAIL、ZD55-TRAIL和PTL联合运用对肝癌细胞株Hep3B和正常肝细胞QSG-7701的增殖抑制作用。结果表明:10 MOI ZD55-TRAIL联合PTL处理72 h后,Hep3B细胞的存活率仅为44%,但对QSG-7701细胞无明显损伤;而10 MOI的ZD55-TRAIL和PTL分别单独处理后细胞存活率为70%和66%;结晶紫实验及Hoechst33342染色结果表明联合处理组的Hep3B细胞凋亡特征更加明显。溶瘤腺病毒ZD55-TRAIL联合PTL在体外对肝癌细胞Hep3B具有显著的杀伤性,而对QSG-7701细胞无明显毒副作用。
基金supported by the Korea Research Institute of Chemical Technology Core Research Program funded by the Ministry of Science&ICT(MSIT,Republic of Korea)(No.KS2522-20)This work was also supported by the Program of the National Research Foundation(NRF)funded by the MSIT(No.2022M3H4A3A01083536 and RS-2024-00466554).
文摘While unitized regenerative fuel cells(URFCs)are promising for renewable energy storage,their efficient operation requires simultaneous water management and gas transport,which is challenging from the standpoint of water management.Herein,a novel approach is introduced for examining the alignment hydrophilic pattern of a Ti porous transport layer(PTL)with the flow field of a bipolar plate(BP).UV/ozone patterning and is employed to impart amphiphilic characteristics to the hydrophobic silanized Ti PTL,enabling low-cost and scalable fabrication.The hydrophilic pattern and its alignment with the BP are comprehensively analyzed using electrochemical methods and computational simulations.Notably,the serpentine-patterned(SP)Ti PTL,wherein the hydrophilic channel is directly aligned with the serpentine flow field of the BP,effectively enhances oxygen removal in the water electrolyzer(WE)mode and mitigates water flooding in the fuel cell(FC)mode,ensuring uninterrupted water and gas flow.Further,URFCs with SP configuration exhibit remarkable performance in the WE and FC modes,achieving a significantly improved round-trip efficiency of 25.7%at 2 A cm^(−2).
