A new compound Ce_(2)PtGe_(3) was synthesized using arc-melting process.The compound crystallizes in an orthorhombic Cmce crystal structure with lattice parameters a=8.5157(17)Å,b=14.7496(29)Å and c=4.2511(9...A new compound Ce_(2)PtGe_(3) was synthesized using arc-melting process.The compound crystallizes in an orthorhombic Cmce crystal structure with lattice parameters a=8.5157(17)Å,b=14.7496(29)Å and c=4.2511(9)Å.The structure of Ce_(2)PtGe_(3) can be derived from the structure of YAlGe,which is another superstructure derivative of the AlB_(2) prototype crystallizing in the Cmcm space group.The crystal structure of Ce_(2)PtGe_(3) is composed of infinite arrays of hexagonal[Pt_(3)Ge_(3)]units stacked along the b-axis and the Ce sites are sandwiched between these parallel hexagonal networks,which is closely related to the other known orthorhombic superstructures Ca_(2)AgSi_(3)(SG:Fmmm)and Ba_(2)LiSi_(3)(SG:Fddd)in the AlB_(2) family.Both DC and AC susceptibility measurements reveal a spin glass state probably arising from nonmagnetic atomic disorder(NMAD)from positional disorder from a part of Pt and Ge crystallographic sites.This was further supported by specific heat capacity measurements with a high value of γ=125 mJ mol^(-1) K^(-2).The modified Curie-Weiss fitting yielded a residual magnetic moment(χ_(0))0.0011 emu mol^(-1),and Curie paramagnetic temperature,θ_(p)=^(-2)1.5 K suggesting antiferromagnetic coupling and an effective magnetic moment of 3.09μB per Ce which indicates the presence of Ce^(3+).A thorough analysis of the temperature dependent resistivity data revealed that the compound might be a Kondo semiconductor at low temperature.展开更多
Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient trans...Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene expression.We established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection efficiency.Furthermore,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and turnip.Finally,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.展开更多
基金the Jawaharlal Nehru Centre for Advanced Scientific Research,Sheikh Saqr Laboratory and Department of Science and Technology,India(DST),Council of Scientific and Industrial Research(CSIR)(01(2787)/14/EMR-Ⅱ)for financial supportthe CSIR for a research fellowship+1 种基金a UGC fellowshipthe UGC-DAE CSR,Mumbai center for a project fellowship(Project No.CRS-M-166).
文摘A new compound Ce_(2)PtGe_(3) was synthesized using arc-melting process.The compound crystallizes in an orthorhombic Cmce crystal structure with lattice parameters a=8.5157(17)Å,b=14.7496(29)Å and c=4.2511(9)Å.The structure of Ce_(2)PtGe_(3) can be derived from the structure of YAlGe,which is another superstructure derivative of the AlB_(2) prototype crystallizing in the Cmcm space group.The crystal structure of Ce_(2)PtGe_(3) is composed of infinite arrays of hexagonal[Pt_(3)Ge_(3)]units stacked along the b-axis and the Ce sites are sandwiched between these parallel hexagonal networks,which is closely related to the other known orthorhombic superstructures Ca_(2)AgSi_(3)(SG:Fmmm)and Ba_(2)LiSi_(3)(SG:Fddd)in the AlB_(2) family.Both DC and AC susceptibility measurements reveal a spin glass state probably arising from nonmagnetic atomic disorder(NMAD)from positional disorder from a part of Pt and Ge crystallographic sites.This was further supported by specific heat capacity measurements with a high value of γ=125 mJ mol^(-1) K^(-2).The modified Curie-Weiss fitting yielded a residual magnetic moment(χ_(0))0.0011 emu mol^(-1),and Curie paramagnetic temperature,θ_(p)=^(-2)1.5 K suggesting antiferromagnetic coupling and an effective magnetic moment of 3.09μB per Ce which indicates the presence of Ce^(3+).A thorough analysis of the temperature dependent resistivity data revealed that the compound might be a Kondo semiconductor at low temperature.
基金financially supported by the Key project of National Natural Science Foundation of China (Grant No.32330096)Innovative Research Group Project of Hebei Natural Science Foundation (Grant No.C2024204246)+3 种基金S&T Program of Hebei (Grant Nos.21372901D23567601H)Natural Science Foundation of Hebei (Grant No.C2023204119)the Starting Grant from Hebei Agricultural University (Grant No.YJ201958)。
文摘Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene expression.We established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection efficiency.Furthermore,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and turnip.Finally,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.
