Pea seed-borne mosaic virus(PSbMV)is a significant pathogen that affects the yield and quality of leguminous crops.In recent years,PSbMV has emerged as the dominant viral threat to legume cultivation in Yunnan Provinc...Pea seed-borne mosaic virus(PSbMV)is a significant pathogen that affects the yield and quality of leguminous crops.In recent years,PSbMV has emerged as the dominant viral threat to legume cultivation in Yunnan Province,China.In this study,the complete genome of PSbMV-YN isolate(GenBank accession:PP746839)with a length of 9919 nucleotides excluding the poly(A)tail was obtained by segmented amplification combined with rapid amplification of cDNA ends(RACE).Phylogenomic analysis of full-length polyprotein-encoding nucleotide and predicted amino acid sequences revealed that PSbMV-YN clusters within the P1 pathotype clade.Genetic mapping of local pea varieties demonstrated eIF4E,a gene tightly linked to the sbm1 locus,as the susceptibility genotype,revealing the mechanistic basis for the prevalence of the P1 pathotype in Yunnan pea populations.Further analysis of the highly variable region of the P1 cistron in PSbMV-YN indicates that the P1 protein could be classified as Type A,which generally lacks RNA silencing suppressor activity.Using a GFP suppression assay,we further validated the RNA silencing suppressor activity of HC-Pro in both Nicotiana benthamiana and its natural host—Pisum sativum L.Additionally,immunoprecipitation sequencing(IP-seq)revealed that HC-Pro binds to small RNAs of variable lengths,thereby effectively inhibiting both local and systemic RNA silencing to promote viral infection.This study reports the first genomic sequence of a Chinese isolate of PSbMV and provides a comprehensive analysis of interactions between HC-Pro with endogenous small RNAs.展开更多
基金supported by the National Natural Science Foundation of China(32025031 and 32170167)the Yunnan Provincial Science and Technology Talent and Platform Program(Expert Workstation)(202405AF140083).
文摘Pea seed-borne mosaic virus(PSbMV)is a significant pathogen that affects the yield and quality of leguminous crops.In recent years,PSbMV has emerged as the dominant viral threat to legume cultivation in Yunnan Province,China.In this study,the complete genome of PSbMV-YN isolate(GenBank accession:PP746839)with a length of 9919 nucleotides excluding the poly(A)tail was obtained by segmented amplification combined with rapid amplification of cDNA ends(RACE).Phylogenomic analysis of full-length polyprotein-encoding nucleotide and predicted amino acid sequences revealed that PSbMV-YN clusters within the P1 pathotype clade.Genetic mapping of local pea varieties demonstrated eIF4E,a gene tightly linked to the sbm1 locus,as the susceptibility genotype,revealing the mechanistic basis for the prevalence of the P1 pathotype in Yunnan pea populations.Further analysis of the highly variable region of the P1 cistron in PSbMV-YN indicates that the P1 protein could be classified as Type A,which generally lacks RNA silencing suppressor activity.Using a GFP suppression assay,we further validated the RNA silencing suppressor activity of HC-Pro in both Nicotiana benthamiana and its natural host—Pisum sativum L.Additionally,immunoprecipitation sequencing(IP-seq)revealed that HC-Pro binds to small RNAs of variable lengths,thereby effectively inhibiting both local and systemic RNA silencing to promote viral infection.This study reports the first genomic sequence of a Chinese isolate of PSbMV and provides a comprehensive analysis of interactions between HC-Pro with endogenous small RNAs.
