Prostate-specific membrane antigen(PSMA)is known to be overexpressed in prostate cancer(PCa).The development of precise and rapid imaging technologies to monitor PSMA is crucial for early diagnosis and therapy.Fluores...Prostate-specific membrane antigen(PSMA)is known to be overexpressed in prostate cancer(PCa).The development of precise and rapid imaging technologies to monitor PSMA is crucial for early diagnosis and therapy.Fluorescence imaging in the second near-infrared window(NIR-II)has emerged as a powerful tool for real-time tracking and in vivo visualization,offering high sensitivity and resolu-tion.However,there is a lack of stable,bright and easy-to-implement NIR-II fluorescent probes for PSMA targeting.Herein,we presented a PSMA-targeting NIR-II fluorescent probe FC-PSMA based onπ-conjugated crossbreeding dyed strategy that affords high stability,large extinction coefficient,and good bright-ness.As demonstrated,FC-PSMA displayed a high fluorescence quantum yield in fetal bovine serum(FBS).Following intravenous injection of FC-PSMA,the tumor-to-normal ratio of fluorescence intensity steadily increased over time,reaching a peak at 48 h(tumor-to-leg ratio=12.16±0.90).This advancement enables precise identification of PC through NIR-II fluorescence imaging,facili-tating high-performance guidance for prostate cancer resection surgery.展开更多
基金supported by NSFC/China(22225805,21878087,22378122,32394001,and 32121005)National Key Research and Development Program(2021YFA0910000),Shanghai Science and Technology Innovation Action Plan(No.23J21901600)+4 种基金Innovation Program of Shanghai Municipal Education Commission,Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism(Shanghai Municipal Education Commission,grant 2021 Sci&Tech 03-28)Shanghai Municipal Science and Technology Major Project(Grant 2018SHZDZX03)Shanghai Science and Technology Committee Rising-Star Program(22QC1400400)Programme of Introducing Talents of Discipline to Universities(B16017)Natural Science Foundation of Shanghai(23ZR1416600).
文摘Prostate-specific membrane antigen(PSMA)is known to be overexpressed in prostate cancer(PCa).The development of precise and rapid imaging technologies to monitor PSMA is crucial for early diagnosis and therapy.Fluorescence imaging in the second near-infrared window(NIR-II)has emerged as a powerful tool for real-time tracking and in vivo visualization,offering high sensitivity and resolu-tion.However,there is a lack of stable,bright and easy-to-implement NIR-II fluorescent probes for PSMA targeting.Herein,we presented a PSMA-targeting NIR-II fluorescent probe FC-PSMA based onπ-conjugated crossbreeding dyed strategy that affords high stability,large extinction coefficient,and good bright-ness.As demonstrated,FC-PSMA displayed a high fluorescence quantum yield in fetal bovine serum(FBS).Following intravenous injection of FC-PSMA,the tumor-to-normal ratio of fluorescence intensity steadily increased over time,reaching a peak at 48 h(tumor-to-leg ratio=12.16±0.90).This advancement enables precise identification of PC through NIR-II fluorescence imaging,facili-tating high-performance guidance for prostate cancer resection surgery.
