Objective:Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders.Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties.We investigated the protective effect...Objective:Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders.Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties.We investigated the protective effects of aqueous extract(AE)and methanolic extract(ME)of A.melegueta on the ejaculatory function of hypothyroid male rats.Methods:Forty sexually experienced male rats were partitioned into 8 groups(5 rats per group)and treated for 28 d as follows:Group 1,Control;Group 2,propylthiouracil(PTU,10 mg/kg)+distilled water(DW,10 m L/kg);Group 3,PTU+5%Tween 80(10 m L/kg);Group 4,PTU+bromocriptine(6 mg/kg);Group 5,PTU+AE(20 mg/kg);Group 6,PTU+AE(100 mg/kg);Group 7,PTU+ME(20 mg/kg),and Group 8,PTU+ME(100 mg/kg).On days 0,7,14 and 28 of treatment,each male rat was paired with primed receptive female for measurement of ejaculatory latency time(ELT)and post-ejaculatory interval(PEI)for1.5 h.On day 29,each male rat was urethane-anesthetized and the spinal cord was transected.Thereafter,following urethral/penile stimulations and intravenous injection of dopamine,contractions of the bulbospongiosus muscles and the intraseminal pressure were registered.After these recordings,blood was collected through the catheterization of abdominal artery and plasma was used for thyroidstimulating hormone(TSH),prolactin and testosterone assays.Results:PTU-induced hypothyroidism was characterized by a significant elevation(P<0.001)of plasmatic TSH and prolactin levels,but a decline(P<0.001)in plasmatic testosterone,compared to untreated group.ELT,PEI,contractions of the bulbospongiosus muscles and the intraseminal pressure were also altered by PTU treatment.On the contrary,A.melegueta extracts elevated testosterone(AE,100 mg/kg,P<0.01;ME,100 mg/kg,P<0.05)and decreased prolactin(AE,100 mg/kg,P<0.05;ME,20 mg/kg,P<0.05)levels,compared to corresponding controls.With regard to DW+PTU group,prolactin concentration was lowered(P<0.05)in rats administered with bromocriptine.Treatment with A.melegueta extracts significantly prevented the lengthening of ELT(P<0.05)and PEI(P<0.001).Hypothyroid state also altered the fictive ejaculation by increasing the latency and decreasing the number and frequency of bulbospongiosus muscle contractions.There was also a decrease in the intraseminal pressure.These alterations were significantly(P<0.05)alleviated in plant extract-treated groups.Conclusion:This study highlighted the ejaculatory disturbance of hypothyroidism in male rats and its prevention with A.melegueta extracts.展开更多
Antithyroid drugs are molecules known as thionamides that inhibit thyroid hormone synthesis by interfering with thyroid peroxidase mediated iodination of tyrosine residues in thyroglobulin. These extensively used drug...Antithyroid drugs are molecules known as thionamides that inhibit thyroid hormone synthesis by interfering with thyroid peroxidase mediated iodination of tyrosine residues in thyroglobulin. These extensively used drugs are associated with a variety of well-known side effects such as anti-neutrophil cytoplasmic antibody （ANCA）-positive vasculitis, granulocytopenia and aplastic anemia. Recently, an atypical hematological finding -- bone marrow plasmacytosis, related to the use of methimazole -- was reported twice in English literatures, but bone marrow plasmacytosis with the use of propylthiouracil （PTU） has hardly been reported so far. Herein we present a case of a patient with Graves＇ disease who was initially investigated for plasma cell dyscrasia but finally diagnosed as PTU-induced bone marrow plasmacytosis with granulocytopenia and ANCA-associated vasculitis.展开更多
目的比较2种不同抗甲状腺药物治疗甲状腺功能亢进症的疗效,为临床用药提供参考。方法 120例初诊的甲亢患者按随机分组的原则随机分成甲巯咪唑(M M I,商品名:赛治)组和丙硫氧嘧啶(PTU)组,每组60例,随诊观察2年后比较各自的临床改善情况...目的比较2种不同抗甲状腺药物治疗甲状腺功能亢进症的疗效,为临床用药提供参考。方法 120例初诊的甲亢患者按随机分组的原则随机分成甲巯咪唑(M M I,商品名:赛治)组和丙硫氧嘧啶(PTU)组,每组60例,随诊观察2年后比较各自的临床改善情况。结果与PTU组比较,MMI组临床症状控制时间较短[(6.9±3.7)周vs.(9.8±3.5)周,P<0.05],TSH恢复正常较快[(7.5±4.1)个月vs.(10.3±5.2)个月,P<0.05],且依从性好,治疗2年后的停药人数较多(26 vs.18,P<0.05)。两组白细胞减少、皮疹、甲状腺功能减退、胃肠道反应发生率比较差异无统计学意义(P>0.05);MMI组的ALT、AST分别为(38.2±7.9)、(37.8±6.5)U/L,且均低于PTU组[(73.6±12.5)、(69.6±10.3)U/L],差异有统计学意义(P<0.05)。结论 M M I、PTU对甲亢的治疗均有效,但MMI组临床症状控制快,TSH恢复正常快,依从性好,停药早,肝功能损害小,整体疗效较好。展开更多
目的观察甲巯咪唑与丙基硫氧嘧啶对甲亢继发骨质疏松患者骨密度的影响,为临床甲亢继发骨质疏松的治疗提供参考。方法选取2012年12月至2013年12月来我科住院治疗的46例甲亢继发骨质疏松患者,采用随机数字法平均分为观察组和对照组。在常...目的观察甲巯咪唑与丙基硫氧嘧啶对甲亢继发骨质疏松患者骨密度的影响,为临床甲亢继发骨质疏松的治疗提供参考。方法选取2012年12月至2013年12月来我科住院治疗的46例甲亢继发骨质疏松患者,采用随机数字法平均分为观察组和对照组。在常规治疗基础上,观察组采用甲巯咪唑进行治疗,对照组使用丙基硫氧嘧啶进行治疗。比较两组治疗前后的血钙(Ca)、血镁(Mg)、血磷(P)、血碱性磷酸酶(ALP)、24 h尿钙(UCa)变化;于治疗前后测量患者腰椎、股骨颈骨密度(BMD),分析甲巯咪唑与丙基硫氧嘧啶对甲亢继发骨质疏松患者骨密度的影响;并比较两组药物不良反应的发生情况。结果观察组治疗后24 h UCa水平明显低于对照组,差异有统计学意义(P<0.05);观察组治疗后Mg水平明显高于对照组,差异有统计学意义(P<0.05)。观察组治疗后Ca、P、ALP水平与对照组比较差异无统计学意义(P>0.05)。观察组治疗后腰椎、股骨颈骨密度均略高于对照组,但差异无统计学意义(P>0.05)。对照组肝功能损害的发生率明显高于观察组,差异有统计学意义(P<0.05)。结论甲巯咪唑和丙基硫氧嘧啶均可以改善甲亢继发骨质疏松患者的骨代谢状态,但甲巯咪唑升高患者骨密度的作用更明显,且不良反应相对较轻,值得临床借鉴使用。展开更多
目的通过观察海藻与甘草不同比例配伍的海藻玉壶汤对丙硫氧嘧啶造成的甲状腺肿大大鼠模型生物效应的影响,筛选出海藻与甘草配比起效的适宜条件,并对其作用机制进行初步探讨。方法将海藻与甘草按照2因素7水平的均匀设计实验原则设置不同...目的通过观察海藻与甘草不同比例配伍的海藻玉壶汤对丙硫氧嘧啶造成的甲状腺肿大大鼠模型生物效应的影响,筛选出海藻与甘草配比起效的适宜条件,并对其作用机制进行初步探讨。方法将海藻与甘草按照2因素7水平的均匀设计实验原则设置不同配伍比例的海藻玉壶汤,Wistar大鼠120只,随机分为10组,采用ig丙硫氧嘧啶14 d复制动物模型,造模后各组ig给药28 d,实验结束后测定大鼠甲状腺质量,测定血清中三碘甲腺原氨酸(T3)、四碘甲腺原氨酸(T4)、促甲状腺激素(TSH)等甲状腺激素水平,RT-PCR法测定大鼠甲状腺中甲状腺过氧化物酶(TPO)和甲状腺球蛋白(Tg)的m RNA表达。结果海藻玉壶汤中海藻与甘草不同配比在不同程度上均有调节甲状腺激素水平、改善丙硫氧嘧啶所致的甲状腺肿大作用。与模型组比较,配比5组(0.135∶8.32)较模型组TPO m RNA的表达显著升高。海藻玉壶汤配比1组(14.522∶6.24)、5组、6组(29.040∶4.16)的Tg m RNA水平较对照组显著升高。结论海藻玉壶汤中海藻与甘草不同配比在不同程度上均能抑制丙硫氧嘧啶所导致的甲状腺肿大,以配比1组、3组(19.363∶0.54)、6组表现最为明显,其作用机制可能涉及刺激TPO与Tg基因转录的增强。配比1组与配比5组具有促进这种代偿增强的作用,在某种程度上对甲状腺激素水平的恢复起到积极的作用。展开更多
文摘Objective:Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders.Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties.We investigated the protective effects of aqueous extract(AE)and methanolic extract(ME)of A.melegueta on the ejaculatory function of hypothyroid male rats.Methods:Forty sexually experienced male rats were partitioned into 8 groups(5 rats per group)and treated for 28 d as follows:Group 1,Control;Group 2,propylthiouracil(PTU,10 mg/kg)+distilled water(DW,10 m L/kg);Group 3,PTU+5%Tween 80(10 m L/kg);Group 4,PTU+bromocriptine(6 mg/kg);Group 5,PTU+AE(20 mg/kg);Group 6,PTU+AE(100 mg/kg);Group 7,PTU+ME(20 mg/kg),and Group 8,PTU+ME(100 mg/kg).On days 0,7,14 and 28 of treatment,each male rat was paired with primed receptive female for measurement of ejaculatory latency time(ELT)and post-ejaculatory interval(PEI)for1.5 h.On day 29,each male rat was urethane-anesthetized and the spinal cord was transected.Thereafter,following urethral/penile stimulations and intravenous injection of dopamine,contractions of the bulbospongiosus muscles and the intraseminal pressure were registered.After these recordings,blood was collected through the catheterization of abdominal artery and plasma was used for thyroidstimulating hormone(TSH),prolactin and testosterone assays.Results:PTU-induced hypothyroidism was characterized by a significant elevation(P<0.001)of plasmatic TSH and prolactin levels,but a decline(P<0.001)in plasmatic testosterone,compared to untreated group.ELT,PEI,contractions of the bulbospongiosus muscles and the intraseminal pressure were also altered by PTU treatment.On the contrary,A.melegueta extracts elevated testosterone(AE,100 mg/kg,P<0.01;ME,100 mg/kg,P<0.05)and decreased prolactin(AE,100 mg/kg,P<0.05;ME,20 mg/kg,P<0.05)levels,compared to corresponding controls.With regard to DW+PTU group,prolactin concentration was lowered(P<0.05)in rats administered with bromocriptine.Treatment with A.melegueta extracts significantly prevented the lengthening of ELT(P<0.05)and PEI(P<0.001).Hypothyroid state also altered the fictive ejaculation by increasing the latency and decreasing the number and frequency of bulbospongiosus muscle contractions.There was also a decrease in the intraseminal pressure.These alterations were significantly(P<0.05)alleviated in plant extract-treated groups.Conclusion:This study highlighted the ejaculatory disturbance of hypothyroidism in male rats and its prevention with A.melegueta extracts.
文摘Antithyroid drugs are molecules known as thionamides that inhibit thyroid hormone synthesis by interfering with thyroid peroxidase mediated iodination of tyrosine residues in thyroglobulin. These extensively used drugs are associated with a variety of well-known side effects such as anti-neutrophil cytoplasmic antibody （ANCA）-positive vasculitis, granulocytopenia and aplastic anemia. Recently, an atypical hematological finding -- bone marrow plasmacytosis, related to the use of methimazole -- was reported twice in English literatures, but bone marrow plasmacytosis with the use of propylthiouracil （PTU） has hardly been reported so far. Herein we present a case of a patient with Graves＇ disease who was initially investigated for plasma cell dyscrasia but finally diagnosed as PTU-induced bone marrow plasmacytosis with granulocytopenia and ANCA-associated vasculitis.
文摘目的比较2种不同抗甲状腺药物治疗甲状腺功能亢进症的疗效,为临床用药提供参考。方法 120例初诊的甲亢患者按随机分组的原则随机分成甲巯咪唑(M M I,商品名:赛治)组和丙硫氧嘧啶(PTU)组,每组60例,随诊观察2年后比较各自的临床改善情况。结果与PTU组比较,MMI组临床症状控制时间较短[(6.9±3.7)周vs.(9.8±3.5)周,P<0.05],TSH恢复正常较快[(7.5±4.1)个月vs.(10.3±5.2)个月,P<0.05],且依从性好,治疗2年后的停药人数较多(26 vs.18,P<0.05)。两组白细胞减少、皮疹、甲状腺功能减退、胃肠道反应发生率比较差异无统计学意义(P>0.05);MMI组的ALT、AST分别为(38.2±7.9)、(37.8±6.5)U/L,且均低于PTU组[(73.6±12.5)、(69.6±10.3)U/L],差异有统计学意义(P<0.05)。结论 M M I、PTU对甲亢的治疗均有效,但MMI组临床症状控制快,TSH恢复正常快,依从性好,停药早,肝功能损害小,整体疗效较好。
文摘目的观察甲巯咪唑与丙基硫氧嘧啶对甲亢继发骨质疏松患者骨密度的影响,为临床甲亢继发骨质疏松的治疗提供参考。方法选取2012年12月至2013年12月来我科住院治疗的46例甲亢继发骨质疏松患者,采用随机数字法平均分为观察组和对照组。在常规治疗基础上,观察组采用甲巯咪唑进行治疗,对照组使用丙基硫氧嘧啶进行治疗。比较两组治疗前后的血钙(Ca)、血镁(Mg)、血磷(P)、血碱性磷酸酶(ALP)、24 h尿钙(UCa)变化;于治疗前后测量患者腰椎、股骨颈骨密度(BMD),分析甲巯咪唑与丙基硫氧嘧啶对甲亢继发骨质疏松患者骨密度的影响;并比较两组药物不良反应的发生情况。结果观察组治疗后24 h UCa水平明显低于对照组,差异有统计学意义(P<0.05);观察组治疗后Mg水平明显高于对照组,差异有统计学意义(P<0.05)。观察组治疗后Ca、P、ALP水平与对照组比较差异无统计学意义(P>0.05)。观察组治疗后腰椎、股骨颈骨密度均略高于对照组,但差异无统计学意义(P>0.05)。对照组肝功能损害的发生率明显高于观察组,差异有统计学意义(P<0.05)。结论甲巯咪唑和丙基硫氧嘧啶均可以改善甲亢继发骨质疏松患者的骨代谢状态,但甲巯咪唑升高患者骨密度的作用更明显,且不良反应相对较轻,值得临床借鉴使用。
文摘目的通过观察海藻与甘草不同比例配伍的海藻玉壶汤对丙硫氧嘧啶造成的甲状腺肿大大鼠模型生物效应的影响,筛选出海藻与甘草配比起效的适宜条件,并对其作用机制进行初步探讨。方法将海藻与甘草按照2因素7水平的均匀设计实验原则设置不同配伍比例的海藻玉壶汤,Wistar大鼠120只,随机分为10组,采用ig丙硫氧嘧啶14 d复制动物模型,造模后各组ig给药28 d,实验结束后测定大鼠甲状腺质量,测定血清中三碘甲腺原氨酸(T3)、四碘甲腺原氨酸(T4)、促甲状腺激素(TSH)等甲状腺激素水平,RT-PCR法测定大鼠甲状腺中甲状腺过氧化物酶(TPO)和甲状腺球蛋白(Tg)的m RNA表达。结果海藻玉壶汤中海藻与甘草不同配比在不同程度上均有调节甲状腺激素水平、改善丙硫氧嘧啶所致的甲状腺肿大作用。与模型组比较,配比5组(0.135∶8.32)较模型组TPO m RNA的表达显著升高。海藻玉壶汤配比1组(14.522∶6.24)、5组、6组(29.040∶4.16)的Tg m RNA水平较对照组显著升高。结论海藻玉壶汤中海藻与甘草不同配比在不同程度上均能抑制丙硫氧嘧啶所导致的甲状腺肿大,以配比1组、3组(19.363∶0.54)、6组表现最为明显,其作用机制可能涉及刺激TPO与Tg基因转录的增强。配比1组与配比5组具有促进这种代偿增强的作用,在某种程度上对甲状腺激素水平的恢复起到积极的作用。
